Isolation, molecular cloning and antimicrobial activity of novel defensins from common chickweed (Stellaria media L.) seeds

Two novel highly homologous defensins, Sm-AMP-D1 and Sm-AMP-D2, were isolated from seeds of common chickweed Stellaria media L. (family Cariophyllaceae). They show sequence homology to defensins of the Brassicaceae plants and display strong inhibitory activity against phytopathogenic fungi and oomyc...

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Veröffentlicht in:	Biochimie 2011-03, Vol.93 (3), p.450-456
Hauptverfasser:	Slavokhotova, Anna A., Odintsova, Tatyana I., Rogozhin, Eugene A., Musolyamov, Alexander K., Andreev, Yaroslav A., Grishin, Eugene V., Egorov, Tsezi A.
Format:	Artikel
Sprache:	eng
Schlagworte:	3' Untranslated regions Amino Acid Sequence Amino acids Antifungal activity Antifungal Agents - chemistry Antifungal Agents - isolation & purification Antifungal Agents - pharmacology Antimicrobial activity Base Sequence Brassicaceae cDNA cloning Cloning, Molecular Crops Defensin Defensins Defensins - chemistry Defensins - genetics Defensins - isolation & purification Defensins - pharmacology DNA, Complementary - genetics Fungi - drug effects Gene structure Genome, Plant - genetics Genomics Homology Introns Media (isolation) Molecular Sequence Data Nucleotide sequence Oomycetes Pathogens Phytopathogenic fungi Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - isolation & purification Plant Proteins - pharmacology Polymerase chain reaction Primers Seeds Seeds - chemistry Seeds - genetics Sequence Analysis, DNA Signal peptides Stellaria - chemistry Stellaria - genetics Stellaria media Stellaria media (L.) Vill Weeds
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