Isolation, molecular cloning and antimicrobial activity of novel defensins from common chickweed (Stellaria media L.) seeds
Two novel highly homologous defensins, Sm-AMP-D1 and Sm-AMP-D2, were isolated from seeds of common chickweed Stellaria media L. (family Cariophyllaceae). They show sequence homology to defensins of the Brassicaceae plants and display strong inhibitory activity against phytopathogenic fungi and oomyc...
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| Veröffentlicht in: | Biochimie 2011-03, Vol.93 (3), p.450-456 |
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| creator | Slavokhotova, Anna A. Odintsova, Tatyana I. Rogozhin, Eugene A. Musolyamov, Alexander K. Andreev, Yaroslav A. Grishin, Eugene V. Egorov, Tsezi A. |
| description | Two novel highly homologous defensins, Sm-AMP-D1 and Sm-AMP-D2, were isolated from seeds of common chickweed Stellaria media L. (family Cariophyllaceae). They show sequence homology to defensins of the Brassicaceae plants and display strong inhibitory activity against phytopathogenic fungi and oomycetes in the micromolar range (IC50≤1μM). The cDNA sequences coding for Sm-AMP-D1 and Sm-AMP-D2 were obtained. They code for highly homologous precursor proteins, consisting of a signal peptide of 32 amino acid residues and the mature peptide domain of 50 amino acid residues. The Sm-AMP-D1 and Sm-AMP-D2 precursors differ by two amino acids: one in the signal peptide region, and the other, in the mature peptide domain. Two Sm-D1-encoding genes were identified in S. media genome by PCR amplification from the genomic DNA using Sm-D1-specific primers. They contain a single 599-bp intron in the signal peptide domain and differ from each other by nucleotide substitutions in the intron and 3′-untranslated regions, while the coding sequences are well conserved. One of the genes matched perfectly the sm-D1 cDNA sequence. The sm-D genes show promise for engineering pathogen resistance in crops and expand our knowledge on weed genomics.
► Two highly homologous defensins from common chickweed were isolated and sequenced. ► Defensins exhibit strong antifungal activity against important plant pathogens. ► Three defensin-encoding genes were identified, two of which are expressed in seeds. ► Defensins precursors consist of a signal peptide and a mature peptide domains. ► The genomic DNA sequences contain an intron in the signal peptide region. |
| doi_str_mv | 10.1016/j.biochi.2010.10.019 |
| format | Article |
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► Two highly homologous defensins from common chickweed were isolated and sequenced. ► Defensins exhibit strong antifungal activity against important plant pathogens. ► Three defensin-encoding genes were identified, two of which are expressed in seeds. ► Defensins precursors consist of a signal peptide and a mature peptide domains. ► The genomic DNA sequences contain an intron in the signal peptide region.</description><identifier>ISSN: 0300-9084</identifier><identifier>EISSN: 1638-6183</identifier><identifier>DOI: 10.1016/j.biochi.2010.10.019</identifier><identifier>PMID: 21056078</identifier><language>eng</language><publisher>France: Elsevier B.V</publisher><subject>3' Untranslated regions ; Amino Acid Sequence ; Amino acids ; Antifungal activity ; Antifungal Agents - chemistry ; Antifungal Agents - isolation & purification ; Antifungal Agents - pharmacology ; Antimicrobial activity ; Base Sequence ; Brassicaceae ; cDNA cloning ; Cloning, Molecular ; Crops ; Defensin ; Defensins ; Defensins - chemistry ; Defensins - genetics ; Defensins - isolation & purification ; Defensins - pharmacology ; DNA, Complementary - genetics ; Fungi - drug effects ; Gene structure ; Genome, Plant - genetics ; Genomics ; Homology ; Introns ; Media (isolation) ; Molecular Sequence Data ; Nucleotide sequence ; Oomycetes ; Pathogens ; Phytopathogenic fungi ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - isolation & purification ; Plant Proteins - pharmacology ; Polymerase chain reaction ; Primers ; Seeds ; Seeds - chemistry ; Seeds - genetics ; Sequence Analysis, DNA ; Signal peptides ; Stellaria - chemistry ; Stellaria - genetics ; Stellaria media ; Stellaria media (L.) Vill ; Weeds</subject><ispartof>Biochimie, 2011-03, Vol.93 (3), p.450-456</ispartof><rights>2010 Elsevier Masson SAS</rights><rights>Copyright © 2010 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-b12e9698eadc7b76b4b04432a1da4bd6ab5691457ad7b634ff220e6ba38f70f93</citedby><cites>FETCH-LOGICAL-c459t-b12e9698eadc7b76b4b04432a1da4bd6ab5691457ad7b634ff220e6ba38f70f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biochi.2010.10.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21056078$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Slavokhotova, Anna A.</creatorcontrib><creatorcontrib>Odintsova, Tatyana I.</creatorcontrib><creatorcontrib>Rogozhin, Eugene A.</creatorcontrib><creatorcontrib>Musolyamov, Alexander K.</creatorcontrib><creatorcontrib>Andreev, Yaroslav A.</creatorcontrib><creatorcontrib>Grishin, Eugene V.</creatorcontrib><creatorcontrib>Egorov, Tsezi A.</creatorcontrib><title>Isolation, molecular cloning and antimicrobial activity of novel defensins from common chickweed (Stellaria media L.) seeds</title><title>Biochimie</title><addtitle>Biochimie</addtitle><description>Two novel highly homologous defensins, Sm-AMP-D1 and Sm-AMP-D2, were isolated from seeds of common chickweed Stellaria media L. (family Cariophyllaceae). They show sequence homology to defensins of the Brassicaceae plants and display strong inhibitory activity against phytopathogenic fungi and oomycetes in the micromolar range (IC50≤1μM). The cDNA sequences coding for Sm-AMP-D1 and Sm-AMP-D2 were obtained. They code for highly homologous precursor proteins, consisting of a signal peptide of 32 amino acid residues and the mature peptide domain of 50 amino acid residues. The Sm-AMP-D1 and Sm-AMP-D2 precursors differ by two amino acids: one in the signal peptide region, and the other, in the mature peptide domain. Two Sm-D1-encoding genes were identified in S. media genome by PCR amplification from the genomic DNA using Sm-D1-specific primers. They contain a single 599-bp intron in the signal peptide domain and differ from each other by nucleotide substitutions in the intron and 3′-untranslated regions, while the coding sequences are well conserved. One of the genes matched perfectly the sm-D1 cDNA sequence. The sm-D genes show promise for engineering pathogen resistance in crops and expand our knowledge on weed genomics.
► Two highly homologous defensins from common chickweed were isolated and sequenced. ► Defensins exhibit strong antifungal activity against important plant pathogens. ► Three defensin-encoding genes were identified, two of which are expressed in seeds. ► Defensins precursors consist of a signal peptide and a mature peptide domains. ► The genomic DNA sequences contain an intron in the signal peptide region.</description><subject>3' Untranslated regions</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Antifungal activity</subject><subject>Antifungal Agents - chemistry</subject><subject>Antifungal Agents - isolation & purification</subject><subject>Antifungal Agents - pharmacology</subject><subject>Antimicrobial activity</subject><subject>Base Sequence</subject><subject>Brassicaceae</subject><subject>cDNA cloning</subject><subject>Cloning, Molecular</subject><subject>Crops</subject><subject>Defensin</subject><subject>Defensins</subject><subject>Defensins - chemistry</subject><subject>Defensins - genetics</subject><subject>Defensins - isolation & purification</subject><subject>Defensins - pharmacology</subject><subject>DNA, Complementary - genetics</subject><subject>Fungi - drug effects</subject><subject>Gene structure</subject><subject>Genome, Plant - genetics</subject><subject>Genomics</subject><subject>Homology</subject><subject>Introns</subject><subject>Media (isolation)</subject><subject>Molecular Sequence Data</subject><subject>Nucleotide sequence</subject><subject>Oomycetes</subject><subject>Pathogens</subject><subject>Phytopathogenic fungi</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - isolation & purification</subject><subject>Plant Proteins - pharmacology</subject><subject>Polymerase chain reaction</subject><subject>Primers</subject><subject>Seeds</subject><subject>Seeds - chemistry</subject><subject>Seeds - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Signal peptides</subject><subject>Stellaria - chemistry</subject><subject>Stellaria - genetics</subject><subject>Stellaria media</subject><subject>Stellaria media (L.) Vill</subject><subject>Weeds</subject><issn>0300-9084</issn><issn>1638-6183</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EotvCP0DIN0AiYZw4TnxBQhWFSiv1AJwt2xmDl8QudnZR1T9fhy0c6cG2NH7zZvQ-Ql4wqBkw8W5XGx_tD1838KdUA5OPyIaJdqgEG9rHZAMtQCVh4CfkNOcdAHTQyKfkpGHQCeiHDbm9zHHSi4_hLZ3jhHY_6UTtFIMP36kOYzmLn71N0Xg9UW0Xf_DLDY2OhnjAiY7oMGQfMnUpztTGeY6BlsXsz9-II339ZcGpmHpNZxzLva3f0Fx-8jPyxOkp4_P794x8u_j49fxztb36dHn-YVtZ3smlMqxBKeSAerS96YXhBjhvG81Gzc0otOmEZLzr9dgb0XLnmgZQGN0Orgcn2zPy6uh7neKvPeZFzT7bdamAcZ-VhJ51Enj3oHLoWM8ll6snPypLMDkndOo6-VmnG8VArXzUTh35qJXPWi18StvL-wF7U9L41_QXSBG8PwqwBHLwmFS2HoMtySW0ixqj__-EO4N2pF4</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Slavokhotova, Anna A.</creator><creator>Odintsova, Tatyana I.</creator><creator>Rogozhin, Eugene A.</creator><creator>Musolyamov, Alexander K.</creator><creator>Andreev, Yaroslav A.</creator><creator>Grishin, Eugene V.</creator><creator>Egorov, Tsezi A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7T5</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20110301</creationdate><title>Isolation, molecular cloning and antimicrobial activity of novel defensins from common chickweed (Stellaria media L.) seeds</title><author>Slavokhotova, Anna A. ; Odintsova, Tatyana I. ; Rogozhin, Eugene A. ; Musolyamov, Alexander K. ; Andreev, Yaroslav A. ; Grishin, Eugene V. ; Egorov, Tsezi A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-b12e9698eadc7b76b4b04432a1da4bd6ab5691457ad7b634ff220e6ba38f70f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>3' Untranslated regions</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Antifungal activity</topic><topic>Antifungal Agents - chemistry</topic><topic>Antifungal Agents - isolation & purification</topic><topic>Antifungal Agents - pharmacology</topic><topic>Antimicrobial activity</topic><topic>Base Sequence</topic><topic>Brassicaceae</topic><topic>cDNA cloning</topic><topic>Cloning, Molecular</topic><topic>Crops</topic><topic>Defensin</topic><topic>Defensins</topic><topic>Defensins - chemistry</topic><topic>Defensins - genetics</topic><topic>Defensins - isolation & purification</topic><topic>Defensins - pharmacology</topic><topic>DNA, Complementary - genetics</topic><topic>Fungi - drug effects</topic><topic>Gene structure</topic><topic>Genome, Plant - genetics</topic><topic>Genomics</topic><topic>Homology</topic><topic>Introns</topic><topic>Media (isolation)</topic><topic>Molecular Sequence Data</topic><topic>Nucleotide sequence</topic><topic>Oomycetes</topic><topic>Pathogens</topic><topic>Phytopathogenic fungi</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - isolation & purification</topic><topic>Plant Proteins - pharmacology</topic><topic>Polymerase chain reaction</topic><topic>Primers</topic><topic>Seeds</topic><topic>Seeds - chemistry</topic><topic>Seeds - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Signal peptides</topic><topic>Stellaria - chemistry</topic><topic>Stellaria - genetics</topic><topic>Stellaria media</topic><topic>Stellaria media (L.) Vill</topic><topic>Weeds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Slavokhotova, Anna A.</creatorcontrib><creatorcontrib>Odintsova, Tatyana I.</creatorcontrib><creatorcontrib>Rogozhin, Eugene A.</creatorcontrib><creatorcontrib>Musolyamov, Alexander K.</creatorcontrib><creatorcontrib>Andreev, Yaroslav A.</creatorcontrib><creatorcontrib>Grishin, Eugene V.</creatorcontrib><creatorcontrib>Egorov, Tsezi A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Slavokhotova, Anna A.</au><au>Odintsova, Tatyana I.</au><au>Rogozhin, Eugene A.</au><au>Musolyamov, Alexander K.</au><au>Andreev, Yaroslav A.</au><au>Grishin, Eugene V.</au><au>Egorov, Tsezi A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation, molecular cloning and antimicrobial activity of novel defensins from common chickweed (Stellaria media L.) seeds</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>93</volume><issue>3</issue><spage>450</spage><epage>456</epage><pages>450-456</pages><issn>0300-9084</issn><eissn>1638-6183</eissn><abstract>Two novel highly homologous defensins, Sm-AMP-D1 and Sm-AMP-D2, were isolated from seeds of common chickweed Stellaria media L. (family Cariophyllaceae). They show sequence homology to defensins of the Brassicaceae plants and display strong inhibitory activity against phytopathogenic fungi and oomycetes in the micromolar range (IC50≤1μM). The cDNA sequences coding for Sm-AMP-D1 and Sm-AMP-D2 were obtained. They code for highly homologous precursor proteins, consisting of a signal peptide of 32 amino acid residues and the mature peptide domain of 50 amino acid residues. The Sm-AMP-D1 and Sm-AMP-D2 precursors differ by two amino acids: one in the signal peptide region, and the other, in the mature peptide domain. Two Sm-D1-encoding genes were identified in S. media genome by PCR amplification from the genomic DNA using Sm-D1-specific primers. They contain a single 599-bp intron in the signal peptide domain and differ from each other by nucleotide substitutions in the intron and 3′-untranslated regions, while the coding sequences are well conserved. One of the genes matched perfectly the sm-D1 cDNA sequence. The sm-D genes show promise for engineering pathogen resistance in crops and expand our knowledge on weed genomics.
► Two highly homologous defensins from common chickweed were isolated and sequenced. ► Defensins exhibit strong antifungal activity against important plant pathogens. ► Three defensin-encoding genes were identified, two of which are expressed in seeds. ► Defensins precursors consist of a signal peptide and a mature peptide domains. ► The genomic DNA sequences contain an intron in the signal peptide region.</abstract><cop>France</cop><pub>Elsevier B.V</pub><pmid>21056078</pmid><doi>10.1016/j.biochi.2010.10.019</doi><tpages>7</tpages></addata></record> |
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| subjects | 3' Untranslated regions Amino Acid Sequence Amino acids Antifungal activity Antifungal Agents - chemistry Antifungal Agents - isolation & purification Antifungal Agents - pharmacology Antimicrobial activity Base Sequence Brassicaceae cDNA cloning Cloning, Molecular Crops Defensin Defensins Defensins - chemistry Defensins - genetics Defensins - isolation & purification Defensins - pharmacology DNA, Complementary - genetics Fungi - drug effects Gene structure Genome, Plant - genetics Genomics Homology Introns Media (isolation) Molecular Sequence Data Nucleotide sequence Oomycetes Pathogens Phytopathogenic fungi Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - isolation & purification Plant Proteins - pharmacology Polymerase chain reaction Primers Seeds Seeds - chemistry Seeds - genetics Sequence Analysis, DNA Signal peptides Stellaria - chemistry Stellaria - genetics Stellaria media Stellaria media (L.) Vill Weeds |
| title | Isolation, molecular cloning and antimicrobial activity of novel defensins from common chickweed (Stellaria media L.) seeds |
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