Mycoplasma ovis Detected in Free-Living Japanese Serows, Capricornis crispus
Nineteen blood samples collected from free-ranging wild Japanese serows, Capricornis crispus, between 2006 and 2008 in Iwate prefecture were examined for the hemoplasma infection by real-time PCR targeting the 16S rRNA gene. Five (26.3%) out of the 19 samples were positive in real-time PCR with an a...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2011, Vol.73(3), pp.371-373 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | OHTAKE, Yoshihiro NISHIZAWA, Ikuo SATO, Makoto WATANABE, Yusaku NISHIMURA, Takashi MATSUBARA, Kazuei NAGAI, Kazuya HARASAWA, Ryô |
| description | Nineteen blood samples collected from free-ranging wild Japanese serows, Capricornis crispus, between 2006 and 2008 in Iwate prefecture were examined for the hemoplasma infection by real-time PCR targeting the 16S rRNA gene. Five (26.3%) out of the 19 samples were positive in real-time PCR with an average melting temperature at 85.18°C. The positive samples in the real-time PCR were reconfirmed by conventional PCR, and one of them was successful for direct DNA sequencing. The nucleotide sequence of the 16S rRNA gene of the representative stain was identical to that of Mycoplasma ovis. This was the first demonstration of hemotropic mycoplasma infections among the free-living Japanese serows in Japan. |
| doi_str_mv | 10.1292/jvms.10-0383 |
| format | Article |
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Five (26.3%) out of the 19 samples were positive in real-time PCR with an average melting temperature at 85.18°C. The positive samples in the real-time PCR were reconfirmed by conventional PCR, and one of them was successful for direct DNA sequencing. The nucleotide sequence of the 16S rRNA gene of the representative stain was identical to that of Mycoplasma ovis. 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Vet. Med. Sci.</addtitle><description>Nineteen blood samples collected from free-ranging wild Japanese serows, Capricornis crispus, between 2006 and 2008 in Iwate prefecture were examined for the hemoplasma infection by real-time PCR targeting the 16S rRNA gene. Five (26.3%) out of the 19 samples were positive in real-time PCR with an average melting temperature at 85.18°C. The positive samples in the real-time PCR were reconfirmed by conventional PCR, and one of them was successful for direct DNA sequencing. The nucleotide sequence of the 16S rRNA gene of the representative stain was identical to that of Mycoplasma ovis. This was the first demonstration of hemotropic mycoplasma infections among the free-living Japanese serows in Japan.</description><subject>Animals</subject><subject>Capricornis crispus</subject><subject>hemoplasma</subject><subject>Japan - epidemiology</subject><subject>Japanese serow</subject><subject>Mycoplasma</subject><subject>Mycoplasma - classification</subject><subject>Mycoplasma - genetics</subject><subject>Mycoplasma Infections - epidemiology</subject><subject>Mycoplasma Infections - veterinary</subject><subject>Ovis</subject><subject>Phylogeny</subject><subject>Ruminants</subject><issn>0916-7250</issn><issn>1347-7439</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTlv3DAQhYkgQbx20rkOBKRIYznDQzxKZ30GG6RIUhMUd-RoocuktIb_vSnsegs3bjgg5puHmfcIOaVwTplh3zfbNp5TyIFr_o4sKBcqV4Kb92QBhspcsQKOyHGMGwBGhTQfyREDI5mQsCCrX0--HxoXW5f12zpmlziiH3Gd1V12HRDzVb2tu_vspxtchxGzPxj6x3iWLd0Qat-HLg35UMdhip_Ih8o1ET_v6wn5d331d3mbr37f3C0vVrmXVIy5R-4qJ9E7rLBcQ1pZcwW68topKUoNhhVooFBszR0KdCWaNMSQYVlQwU_It53uEPqHCeNo2zp6bJq0YT9Fa0DRQpvibVJL0JopbRL59RW56afQpTNsMk0rBlLIRJ3tKB_6GANWNpnQuvBkKdg5DjvHMX_mOBL-ZS86lS2uD_CL_wn4sQM2cXT3eABcGGvf4E5NccvnZ696aPr_Lljs-DNcz53E</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>OHTAKE, Yoshihiro</creator><creator>NISHIZAWA, Ikuo</creator><creator>SATO, Makoto</creator><creator>WATANABE, Yusaku</creator><creator>NISHIMURA, Takashi</creator><creator>MATSUBARA, Kazuei</creator><creator>NAGAI, Kazuya</creator><creator>HARASAWA, Ryô</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>2011</creationdate><title>Mycoplasma ovis Detected in Free-Living Japanese Serows, Capricornis crispus</title><author>OHTAKE, Yoshihiro ; NISHIZAWA, Ikuo ; SATO, Makoto ; WATANABE, Yusaku ; NISHIMURA, Takashi ; MATSUBARA, Kazuei ; NAGAI, Kazuya ; HARASAWA, Ryô</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c614t-ce3afa6ecaefebd013483708fc8a764b80925e90572d3ae4eabe9ce32e2eb5143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Capricornis crispus</topic><topic>hemoplasma</topic><topic>Japan - epidemiology</topic><topic>Japanese serow</topic><topic>Mycoplasma</topic><topic>Mycoplasma - classification</topic><topic>Mycoplasma - genetics</topic><topic>Mycoplasma Infections - epidemiology</topic><topic>Mycoplasma Infections - veterinary</topic><topic>Ovis</topic><topic>Phylogeny</topic><topic>Ruminants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OHTAKE, Yoshihiro</creatorcontrib><creatorcontrib>NISHIZAWA, Ikuo</creatorcontrib><creatorcontrib>SATO, Makoto</creatorcontrib><creatorcontrib>WATANABE, Yusaku</creatorcontrib><creatorcontrib>NISHIMURA, Takashi</creatorcontrib><creatorcontrib>MATSUBARA, Kazuei</creatorcontrib><creatorcontrib>NAGAI, Kazuya</creatorcontrib><creatorcontrib>HARASAWA, Ryô</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OHTAKE, Yoshihiro</au><au>NISHIZAWA, Ikuo</au><au>SATO, Makoto</au><au>WATANABE, Yusaku</au><au>NISHIMURA, Takashi</au><au>MATSUBARA, Kazuei</au><au>NAGAI, Kazuya</au><au>HARASAWA, Ryô</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mycoplasma ovis Detected in Free-Living Japanese Serows, Capricornis crispus</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2011</date><risdate>2011</risdate><volume>73</volume><issue>3</issue><spage>371</spage><epage>373</epage><pages>371-373</pages><issn>0916-7250</issn><issn>1347-7439</issn><eissn>1347-7439</eissn><abstract>Nineteen blood samples collected from free-ranging wild Japanese serows, Capricornis crispus, between 2006 and 2008 in Iwate prefecture were examined for the hemoplasma infection by real-time PCR targeting the 16S rRNA gene. Five (26.3%) out of the 19 samples were positive in real-time PCR with an average melting temperature at 85.18°C. The positive samples in the real-time PCR were reconfirmed by conventional PCR, and one of them was successful for direct DNA sequencing. The nucleotide sequence of the 16S rRNA gene of the representative stain was identical to that of Mycoplasma ovis. 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| subjects | Animals Capricornis crispus hemoplasma Japan - epidemiology Japanese serow Mycoplasma Mycoplasma - classification Mycoplasma - genetics Mycoplasma Infections - epidemiology Mycoplasma Infections - veterinary Ovis Phylogeny Ruminants |
| title | Mycoplasma ovis Detected in Free-Living Japanese Serows, Capricornis crispus |
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