Association of cytotoxic T lymphocyte associated antigena4 gene (rs60872763) polymorphism with Crohn's disease and high levels of serum sCTLAa4 in Crohn's disease

Association of cytotoxic T lymphocyte associated antigena4 gene (rs60872763) polymorphism with Crohn's disease and high levels of serum sCTLAa4 in Crohn's disease

Background and Aim: Our aim was to evaluate cytotoxic T lymphocyte associated antigen-4 (CTLA-4) gene polymorphisms in Crohn's disease (CD) and explore soluble CTLA-4 (sCTLA-4) levels in serum of CD patients in central China. Methods: A total of 126 Chinese CD patients and 300 healthy controls...

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Veröffentlicht in:Journal of gastroenterology and hepatology 2011-05, Vol.26 (5), p.924-930
Hauptverfasser: Chen, Zhitao, Zhou, Feng, Huang, Sha, Jiang, Ting, Chen, Liping, Ge, Liuqing, Xia, Bing
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container_issue 5
container_start_page 924
container_title Journal of gastroenterology and hepatology
container_volume 26
creator Chen, Zhitao
Zhou, Feng
Huang, Sha
Jiang, Ting
Chen, Liping
Ge, Liuqing
Xia, Bing
description Background and Aim: Our aim was to evaluate cytotoxic T lymphocyte associated antigen-4 (CTLA-4) gene polymorphisms in Crohn's disease (CD) and explore soluble CTLA-4 (sCTLA-4) levels in serum of CD patients in central China. Methods: A total of 126 Chinese CD patients and 300 healthy controls were enrolled in this study. CTLA-4 (AT)n repeat polymorphism was genotyped by a semiautomatic fluorescently labeled polymerase chain reaction (PCR) method, and CTLA-4 -1661A/G and -1722T/C polymorphisms were genotyped by DNA sequencing. Serum sCTLA-4 and C-reactive protein (CRP) levels were determined by enzyme linked immunosorbent assay (ELISA) and immunonephelometry, respectively. Results: The frequency of 84bp allele of CTLA-4 (AT)n repeats was lower in CD patients than in the healthy controls (22.2% vs 33.2%, P=0.001, odds ratio=0.58, 95% confidence interval: 0.41-0.81). The 84bp allele carriers of (AT)n repeats were associated with non-stricturing and non-penetrating disease behavior in CD patients (P=0.007). Serum sCTLA-4 levels were more elevated in CD patients than in the healthy controls (P
doi_str_mv 10.1111/j.1440-1746.2011.06662.x
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Methods: A total of 126 Chinese CD patients and 300 healthy controls were enrolled in this study. CTLA-4 (AT)n repeat polymorphism was genotyped by a semiautomatic fluorescently labeled polymerase chain reaction (PCR) method, and CTLA-4 -1661A/G and -1722T/C polymorphisms were genotyped by DNA sequencing. Serum sCTLA-4 and C-reactive protein (CRP) levels were determined by enzyme linked immunosorbent assay (ELISA) and immunonephelometry, respectively. Results: The frequency of 84bp allele of CTLA-4 (AT)n repeats was lower in CD patients than in the healthy controls (22.2% vs 33.2%, P=0.001, odds ratio=0.58, 95% confidence interval: 0.41-0.81). The 84bp allele carriers of (AT)n repeats were associated with non-stricturing and non-penetrating disease behavior in CD patients (P=0.007). Serum sCTLA-4 levels were more elevated in CD patients than in the healthy controls (P&lt;0.001). Among CD patients, serum sCTLA-4 levels were increased in active disease compared with inactive disease (P=0.015), and were correlated with CRP levels (r=0.524, P&lt;0.001). Serum sCTLA-4 levels were higher in CD patients with stricturing disease behavior than in patients with other disease behaviors (P=0.009). Conclusions: 84bp allele of CTLA-4 (AT)n repeat polymorphism was associated with CD in central China. sCTLA-4 levels were highly expressed in CD, especially in active disease, and were correlated with CRP levels and disease behavior in CD patients.</description><identifier>ISSN: 0815-9319</identifier><identifier>EISSN: 1440-1746</identifier><identifier>DOI: 10.1111/j.1440-1746.2011.06662.x</identifier><language>eng</language><ispartof>Journal of gastroenterology and hepatology, 2011-05, Vol.26 (5), p.924-930</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Chen, Zhitao</creatorcontrib><creatorcontrib>Zhou, Feng</creatorcontrib><creatorcontrib>Huang, Sha</creatorcontrib><creatorcontrib>Jiang, Ting</creatorcontrib><creatorcontrib>Chen, Liping</creatorcontrib><creatorcontrib>Ge, Liuqing</creatorcontrib><creatorcontrib>Xia, Bing</creatorcontrib><title>Association of cytotoxic T lymphocyte associated antigena4 gene (rs60872763) polymorphism with Crohn's disease and high levels of serum sCTLAa4 in Crohn's disease</title><title>Journal of gastroenterology and hepatology</title><description>Background and Aim: Our aim was to evaluate cytotoxic T lymphocyte associated antigen-4 (CTLA-4) gene polymorphisms in Crohn's disease (CD) and explore soluble CTLA-4 (sCTLA-4) levels in serum of CD patients in central China. Methods: A total of 126 Chinese CD patients and 300 healthy controls were enrolled in this study. CTLA-4 (AT)n repeat polymorphism was genotyped by a semiautomatic fluorescently labeled polymerase chain reaction (PCR) method, and CTLA-4 -1661A/G and -1722T/C polymorphisms were genotyped by DNA sequencing. Serum sCTLA-4 and C-reactive protein (CRP) levels were determined by enzyme linked immunosorbent assay (ELISA) and immunonephelometry, respectively. Results: The frequency of 84bp allele of CTLA-4 (AT)n repeats was lower in CD patients than in the healthy controls (22.2% vs 33.2%, P=0.001, odds ratio=0.58, 95% confidence interval: 0.41-0.81). The 84bp allele carriers of (AT)n repeats were associated with non-stricturing and non-penetrating disease behavior in CD patients (P=0.007). Serum sCTLA-4 levels were more elevated in CD patients than in the healthy controls (P&lt;0.001). Among CD patients, serum sCTLA-4 levels were increased in active disease compared with inactive disease (P=0.015), and were correlated with CRP levels (r=0.524, P&lt;0.001). Serum sCTLA-4 levels were higher in CD patients with stricturing disease behavior than in patients with other disease behaviors (P=0.009). 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Methods: A total of 126 Chinese CD patients and 300 healthy controls were enrolled in this study. CTLA-4 (AT)n repeat polymorphism was genotyped by a semiautomatic fluorescently labeled polymerase chain reaction (PCR) method, and CTLA-4 -1661A/G and -1722T/C polymorphisms were genotyped by DNA sequencing. Serum sCTLA-4 and C-reactive protein (CRP) levels were determined by enzyme linked immunosorbent assay (ELISA) and immunonephelometry, respectively. Results: The frequency of 84bp allele of CTLA-4 (AT)n repeats was lower in CD patients than in the healthy controls (22.2% vs 33.2%, P=0.001, odds ratio=0.58, 95% confidence interval: 0.41-0.81). The 84bp allele carriers of (AT)n repeats were associated with non-stricturing and non-penetrating disease behavior in CD patients (P=0.007). Serum sCTLA-4 levels were more elevated in CD patients than in the healthy controls (P&lt;0.001). Among CD patients, serum sCTLA-4 levels were increased in active disease compared with inactive disease (P=0.015), and were correlated with CRP levels (r=0.524, P&lt;0.001). Serum sCTLA-4 levels were higher in CD patients with stricturing disease behavior than in patients with other disease behaviors (P=0.009). Conclusions: 84bp allele of CTLA-4 (AT)n repeat polymorphism was associated with CD in central China. sCTLA-4 levels were highly expressed in CD, especially in active disease, and were correlated with CRP levels and disease behavior in CD patients.</abstract><doi>10.1111/j.1440-1746.2011.06662.x</doi></addata></record>
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title Association of cytotoxic T lymphocyte associated antigena4 gene (rs60872763) polymorphism with Crohn's disease and high levels of serum sCTLAa4 in Crohn's disease
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