Some biomolecules and a partially O-acetylated exo-galactomannan containing β-Galf units from pathogenic Exophiala jeanselmei, having a pronounced immunogenic response
The pathogenic fungus Exophiala jeanselmei (Ej4) was grown in submerged MM medium, glucose being consumed after six days with maximum biomass and EPS production. Cells were extracted with CHCl3-MeOH (2:1, v/v) yielding a product containing 10% lipid, with high levels of unsaturated C(18:1) (43.6%) a...
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| Veröffentlicht in: | International journal of biological macromolecules 2011-01, Vol.48 (1), p.177-182 |
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| creator | Sassaki, G L Czelusniak, P A Vicente, V A Zanata, S M Souza, L M Gorin, P A J Iacomini, M |
| description | The pathogenic fungus Exophiala jeanselmei (Ej4) was grown in submerged MM medium, glucose being consumed after six days with maximum biomass and EPS production. Cells were extracted with CHCl3-MeOH (2:1, v/v) yielding a product containing 10% lipid, with high levels of unsaturated C(18:1) (43.6%) and C(18:2) (21.0%), 2D-TLC showed the presence of PE (17.7%), PS (11.6%), PC (35.8%), PI (1.2%) and lyso-phospholipids, LPE (10.7%), LPC (2.0%), PA (10.4%), cardiolipin (10.5%) and glucosyl-ceramide. Analysis of EPS-1 (120 kDa) showed a galactomanan, containing a main chain of Manp-(1→2) (24.2%), substituted by side chains containing terminal Galf (16.8%) and Manp (3.5%) and acetyl groups attached at O-6 of terminal Galf. An immune response against antigens was obtained using Balb/C mice. Anti-EPS-1 antibodies recognized purified fraction containing cellular walls very titer and higher than 1:20,000 for EPS. The studied biomolecules showed biotechnological potential and point to important perspectives in diagnosis of fungi and immunomodulatory products. |
| doi_str_mv | 10.1016/j.ijbiomac.2010.10.017 |
| format | Article |
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Cells were extracted with CHCl3-MeOH (2:1, v/v) yielding a product containing 10% lipid, with high levels of unsaturated C(18:1) (43.6%) and C(18:2) (21.0%), 2D-TLC showed the presence of PE (17.7%), PS (11.6%), PC (35.8%), PI (1.2%) and lyso-phospholipids, LPE (10.7%), LPC (2.0%), PA (10.4%), cardiolipin (10.5%) and glucosyl-ceramide. Analysis of EPS-1 (120 kDa) showed a galactomanan, containing a main chain of Manp-(1→2) (24.2%), substituted by side chains containing terminal Galf (16.8%) and Manp (3.5%) and acetyl groups attached at O-6 of terminal Galf. An immune response against antigens was obtained using Balb/C mice. Anti-EPS-1 antibodies recognized purified fraction containing cellular walls very titer and higher than 1:20,000 for EPS. The studied biomolecules showed biotechnological potential and point to important perspectives in diagnosis of fungi and immunomodulatory products.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2010.10.017</identifier><identifier>PMID: 21070807</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Acetylation ; Animals ; Antibodies ; Biomass ; cardiolipin ; Chromatography, Gel ; Exophiala - immunology ; Exophiala - pathogenicity ; Exophiala jeanselmei ; Fatty Acids - analysis ; Fungi ; Galactose - chemistry ; Galactose - immunology ; Glucose ; Glycolipids - analysis ; Immune response ; Immunogenicity ; Immunomodulation ; Lipids ; Macromolecules ; Magnetic Resonance Spectroscopy ; Mannans - immunology ; Methylation ; Mice ; Mice, Inbred BALB C ; Phospholipids - analysis ; Polysaccharides - immunology ; Polysaccharides - isolation & purification</subject><ispartof>International journal of biological macromolecules, 2011-01, Vol.48 (1), p.177-182</ispartof><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-5b8e0f6adbfe6c9cdf5bb8c9e38891faf0c0164cd038f51df3938ef37d2221dc3</citedby><cites>FETCH-LOGICAL-c342t-5b8e0f6adbfe6c9cdf5bb8c9e38891faf0c0164cd038f51df3938ef37d2221dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21070807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sassaki, G L</creatorcontrib><creatorcontrib>Czelusniak, P A</creatorcontrib><creatorcontrib>Vicente, V A</creatorcontrib><creatorcontrib>Zanata, S M</creatorcontrib><creatorcontrib>Souza, L M</creatorcontrib><creatorcontrib>Gorin, P A J</creatorcontrib><creatorcontrib>Iacomini, M</creatorcontrib><title>Some biomolecules and a partially O-acetylated exo-galactomannan containing β-Galf units from pathogenic Exophiala jeanselmei, having a pronounced immunogenic response</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>The pathogenic fungus Exophiala jeanselmei (Ej4) was grown in submerged MM medium, glucose being consumed after six days with maximum biomass and EPS production. Cells were extracted with CHCl3-MeOH (2:1, v/v) yielding a product containing 10% lipid, with high levels of unsaturated C(18:1) (43.6%) and C(18:2) (21.0%), 2D-TLC showed the presence of PE (17.7%), PS (11.6%), PC (35.8%), PI (1.2%) and lyso-phospholipids, LPE (10.7%), LPC (2.0%), PA (10.4%), cardiolipin (10.5%) and glucosyl-ceramide. Analysis of EPS-1 (120 kDa) showed a galactomanan, containing a main chain of Manp-(1→2) (24.2%), substituted by side chains containing terminal Galf (16.8%) and Manp (3.5%) and acetyl groups attached at O-6 of terminal Galf. An immune response against antigens was obtained using Balb/C mice. Anti-EPS-1 antibodies recognized purified fraction containing cellular walls very titer and higher than 1:20,000 for EPS. The studied biomolecules showed biotechnological potential and point to important perspectives in diagnosis of fungi and immunomodulatory products.</description><subject>Acetylation</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biomass</subject><subject>cardiolipin</subject><subject>Chromatography, Gel</subject><subject>Exophiala - immunology</subject><subject>Exophiala - pathogenicity</subject><subject>Exophiala jeanselmei</subject><subject>Fatty Acids - analysis</subject><subject>Fungi</subject><subject>Galactose - chemistry</subject><subject>Galactose - immunology</subject><subject>Glucose</subject><subject>Glycolipids - analysis</subject><subject>Immune response</subject><subject>Immunogenicity</subject><subject>Immunomodulation</subject><subject>Lipids</subject><subject>Macromolecules</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mannans - immunology</subject><subject>Methylation</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Phospholipids - analysis</subject><subject>Polysaccharides - immunology</subject><subject>Polysaccharides - isolation & purification</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EotOfV6i8Y0OG63iSOEtUlbZSpS6AdXTjXM84cuwhTlDnjbrmQXgmnHbaLStLR-ecz7qHsUsBawGi_NKvbd_aMKBe5_AsrkFU79hKqKrOAEC-ZysQG5EpIeGEncbYJ7UshPrITnIBFSioVuzpexiIL03BkZ4dRY6-48j3OE4WnTvwhww1TQeHE3WcHkO2RYd6Smzv0XMd_ITWW7_lf_9kN-gMn72dIjdjGFLNtAtb8lbz68ew36VK5D2hj-QGsp_5Dn8v0QQcgw-z1wlih2H2x9RIcR-S-5x9MOgiXRzfM_bz2_WPq9vs_uHm7urrfablJp-yolUEpsSuNVTqWnemaFula5JK1cKgAZ3Ot9EdSGUK0RlZS0VGVl2e56LT8ox9eulN__k1U5yawUZNzqGnMMemhkoUshbVf50qh6quBJTJWb449RhiHMk0-9EOOB4aAc0yZ9M3r3M2y5yLDs-IyyNibgfq3mKv-8l_pBSkPA</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Sassaki, G L</creator><creator>Czelusniak, P A</creator><creator>Vicente, V A</creator><creator>Zanata, S M</creator><creator>Souza, L M</creator><creator>Gorin, P A J</creator><creator>Iacomini, M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope></search><sort><creationdate>20110101</creationdate><title>Some biomolecules and a partially O-acetylated exo-galactomannan containing β-Galf units from pathogenic Exophiala jeanselmei, having a pronounced immunogenic response</title><author>Sassaki, G L ; Czelusniak, P A ; Vicente, V A ; Zanata, S M ; Souza, L M ; Gorin, P A J ; Iacomini, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-5b8e0f6adbfe6c9cdf5bb8c9e38891faf0c0164cd038f51df3938ef37d2221dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acetylation</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Biomass</topic><topic>cardiolipin</topic><topic>Chromatography, Gel</topic><topic>Exophiala - immunology</topic><topic>Exophiala - pathogenicity</topic><topic>Exophiala jeanselmei</topic><topic>Fatty Acids - analysis</topic><topic>Fungi</topic><topic>Galactose - chemistry</topic><topic>Galactose - immunology</topic><topic>Glucose</topic><topic>Glycolipids - analysis</topic><topic>Immune response</topic><topic>Immunogenicity</topic><topic>Immunomodulation</topic><topic>Lipids</topic><topic>Macromolecules</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Mannans - immunology</topic><topic>Methylation</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Phospholipids - analysis</topic><topic>Polysaccharides - immunology</topic><topic>Polysaccharides - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sassaki, G L</creatorcontrib><creatorcontrib>Czelusniak, P A</creatorcontrib><creatorcontrib>Vicente, V A</creatorcontrib><creatorcontrib>Zanata, S M</creatorcontrib><creatorcontrib>Souza, L M</creatorcontrib><creatorcontrib>Gorin, P A J</creatorcontrib><creatorcontrib>Iacomini, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sassaki, G L</au><au>Czelusniak, P A</au><au>Vicente, V A</au><au>Zanata, S M</au><au>Souza, L M</au><au>Gorin, P A J</au><au>Iacomini, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Some biomolecules and a partially O-acetylated exo-galactomannan containing β-Galf units from pathogenic Exophiala jeanselmei, having a pronounced immunogenic response</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>48</volume><issue>1</issue><spage>177</spage><epage>182</epage><pages>177-182</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>The pathogenic fungus Exophiala jeanselmei (Ej4) was grown in submerged MM medium, glucose being consumed after six days with maximum biomass and EPS production. Cells were extracted with CHCl3-MeOH (2:1, v/v) yielding a product containing 10% lipid, with high levels of unsaturated C(18:1) (43.6%) and C(18:2) (21.0%), 2D-TLC showed the presence of PE (17.7%), PS (11.6%), PC (35.8%), PI (1.2%) and lyso-phospholipids, LPE (10.7%), LPC (2.0%), PA (10.4%), cardiolipin (10.5%) and glucosyl-ceramide. Analysis of EPS-1 (120 kDa) showed a galactomanan, containing a main chain of Manp-(1→2) (24.2%), substituted by side chains containing terminal Galf (16.8%) and Manp (3.5%) and acetyl groups attached at O-6 of terminal Galf. An immune response against antigens was obtained using Balb/C mice. Anti-EPS-1 antibodies recognized purified fraction containing cellular walls very titer and higher than 1:20,000 for EPS. The studied biomolecules showed biotechnological potential and point to important perspectives in diagnosis of fungi and immunomodulatory products.</abstract><cop>Netherlands</cop><pmid>21070807</pmid><doi>10.1016/j.ijbiomac.2010.10.017</doi><tpages>6</tpages></addata></record> |
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| subjects | Acetylation Animals Antibodies Biomass cardiolipin Chromatography, Gel Exophiala - immunology Exophiala - pathogenicity Exophiala jeanselmei Fatty Acids - analysis Fungi Galactose - chemistry Galactose - immunology Glucose Glycolipids - analysis Immune response Immunogenicity Immunomodulation Lipids Macromolecules Magnetic Resonance Spectroscopy Mannans - immunology Methylation Mice Mice, Inbred BALB C Phospholipids - analysis Polysaccharides - immunology Polysaccharides - isolation & purification |
| title | Some biomolecules and a partially O-acetylated exo-galactomannan containing β-Galf units from pathogenic Exophiala jeanselmei, having a pronounced immunogenic response |
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