Soluble expression, purification, and characterization of recombinant human flotillin-2 (reggie-1) in Escherichia coli

Large scale production of recombinant human flotillin-2 (reggie-1) is desirable for structural and biochemical studies. However, as the major lipid rafts specific hydrophobic protein, flotillin-2 was difficult to be expressed as soluble and functional form in prokaryotic system. In this study, we fi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Molecular biology reports 2011-03, Vol.38 (3), p.2091-2098
Hauptverfasser:	Song, Jiaping, Chen, Wentao, Lu, Zhisheng, Hu, Xiaojian, Ding, Yu
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity chromatography Animal Anatomy Animal Biochemistry Anions Biomedical and Life Sciences Blotting, Western C.D Circular Dichroism Cloning, Molecular E coli Electrophoresis, Polyacrylamide Gel Escherichia coli Escherichia coli - metabolism Flotillin-1 (reggie 2) Flotillin-2 (reggie 1) Fusion protein Gene expression Genetic recombination Glutathione transferase Histidine - metabolism Histology Humans Hydrophobicity Life Sciences Lipid rafts Lipids maltose-binding protein MBP tag Membrane Proteins - isolation & purification Membrane Proteins - metabolism Molecular biology Morphology Oligopeptides - metabolism Protein structure Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Secondary structure Solubility Spectroscopy Thioredoxin
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!