Calcium Homeostasis in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Rationale Cardiomyocytes generated from human induced pluripotent stem cells (hiPSCs) are suggested as the most promising candidate to replenish cardiomyocyte loss in regenerative medicine. Little is known about their calcium homeostasis, the key process underlying excitation-contraction coupling. O...
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| Veröffentlicht in: | Stem cell reviews 2011-11, Vol.7 (4), p.976-986 |
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| creator | Lee, Yee-Ki Ng, Kwong-Man Lai, Wing-Hon Chan, Yau-Chi Lau, Yee-Man Lian, Qizhou Tse, Hung-Fat Siu, Chung-Wah |
| description | Rationale
Cardiomyocytes generated from human induced pluripotent stem cells (hiPSCs) are suggested as the most promising candidate to replenish cardiomyocyte loss in regenerative medicine. Little is known about their calcium homeostasis, the key process underlying excitation-contraction coupling.
Objective
We investigated the calcium handling properties of hiPSC-derived cardiomyocytes and compared with those from human embryonic stem cells (hESCs).
Methods and Results
We differentiated cardiomyocytes from hiPSCs (IMR90 and KS1) and hESCs (H7 and HES3) with established protocols. Beating outgrowths from embryoid bodies were typically observed 2 weeks after induction. Cells in these outgrowths were stained positively for tropomyosin and sarcomeric alpha-actinin. Reverse-transcription polymerase chain reaction studies demonstrated the expressions of cardiac-specific markers in both hiPSC- and hESC-derived cardiomyocytes. Calcium handling properties of 20-day-old hiPSC- and hESC-derived cardiomyocytes were investigated using fluorescence confocal microscopy. Compared with hESC-derived cardiomyocytes, spontaneous calcium transients from both lines of hiPSC-derived cardiomyocytes were of significantly smaller amplitude and with slower maximal upstroke velocity. Better caffeine-induced calcium handling kinetics in hESC-CMs indicates a higher sacroplasmic recticulum calcium store. Furthermore, in contrast with hESC-derived cardiomyocytes, ryanodine did not reduce the amplitudes, maximal upstroke and decay velocity of calcium transients of hiPSC-derived cardiomyocytes. In addition, spatial inhomogeneity in temporal properties of calcium transients across the width of cardiomyocytes was more pronounced in hiPSC-derived cardiomyocytes than their hESC counterpart as revealed line-scan calcium imaging. Expressions of the key calcium-handling proteins including ryanodine recptor-2 (RyR2), sacroplasmic recticulum calcium-ATPase (SERCA), junction (Jun) and triadin (TRDN), were significantly lower in hiPSC than in hESCs.
Conclusions
The results indicate the calcium handling properties of hiPSC-derived cardiomyocytes are relatively immature to hESC counterparts. |
| doi_str_mv | 10.1007/s12015-011-9273-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_907146207</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2523010291</sourcerecordid><originalsourceid>FETCH-LOGICAL-c468t-6a3772c1abb557f3856a1a5139e0de425535048c091c2dd192ae7cf2ce29b3973</originalsourceid><addsrcrecordid>eNp1kd1rFDEUxYNY7Jd_gC8y-OJTbG6-ZvIiyNS6hUIr1ueQzdytKTOTNZkp7H_fbLfWVvApgfO7597DIeQdsE_AWH2SgTNQlAFQw2tBxStyAEo1VDdMvn74M9oYKfbJYc63jIlGNvCG7HPQIBXoA_K9db0P81At4oAxTy6HXIWxWsyDG6vzsZs9dtVVP6ewjhOOU_VjwqFqse_pKaZwV9TWpS7EYRP9ZsJ8TPZWrs_49vE9Ij_Pvl63C3px-e28_XJBvdTNRLUTdc09uOVSqXolGqUdOAXCIOtQcqWEYrLxzIDnXQeGO6z9invkZilMLY7I553vel4O2PlyW3K9XacwuLSx0QX7UhnDL3sT76zgXGujisHHR4MUf8-YJzuE7EswN2KcszWsBqk526768A95G-c0lnQF0lqKQhUIdpBPMeeEq6dTgNltXXZXly112W1dVpSZ988zPE386acAfAfkIo03mP5u_r_rPaFhoFY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>906643620</pqid></control><display><type>article</type><title>Calcium Homeostasis in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Lee, Yee-Ki ; Ng, Kwong-Man ; Lai, Wing-Hon ; Chan, Yau-Chi ; Lau, Yee-Man ; Lian, Qizhou ; Tse, Hung-Fat ; Siu, Chung-Wah</creator><creatorcontrib>Lee, Yee-Ki ; Ng, Kwong-Man ; Lai, Wing-Hon ; Chan, Yau-Chi ; Lau, Yee-Man ; Lian, Qizhou ; Tse, Hung-Fat ; Siu, Chung-Wah</creatorcontrib><description>Rationale
Cardiomyocytes generated from human induced pluripotent stem cells (hiPSCs) are suggested as the most promising candidate to replenish cardiomyocyte loss in regenerative medicine. Little is known about their calcium homeostasis, the key process underlying excitation-contraction coupling.
Objective
We investigated the calcium handling properties of hiPSC-derived cardiomyocytes and compared with those from human embryonic stem cells (hESCs).
Methods and Results
We differentiated cardiomyocytes from hiPSCs (IMR90 and KS1) and hESCs (H7 and HES3) with established protocols. Beating outgrowths from embryoid bodies were typically observed 2 weeks after induction. Cells in these outgrowths were stained positively for tropomyosin and sarcomeric alpha-actinin. Reverse-transcription polymerase chain reaction studies demonstrated the expressions of cardiac-specific markers in both hiPSC- and hESC-derived cardiomyocytes. Calcium handling properties of 20-day-old hiPSC- and hESC-derived cardiomyocytes were investigated using fluorescence confocal microscopy. Compared with hESC-derived cardiomyocytes, spontaneous calcium transients from both lines of hiPSC-derived cardiomyocytes were of significantly smaller amplitude and with slower maximal upstroke velocity. Better caffeine-induced calcium handling kinetics in hESC-CMs indicates a higher sacroplasmic recticulum calcium store. Furthermore, in contrast with hESC-derived cardiomyocytes, ryanodine did not reduce the amplitudes, maximal upstroke and decay velocity of calcium transients of hiPSC-derived cardiomyocytes. In addition, spatial inhomogeneity in temporal properties of calcium transients across the width of cardiomyocytes was more pronounced in hiPSC-derived cardiomyocytes than their hESC counterpart as revealed line-scan calcium imaging. Expressions of the key calcium-handling proteins including ryanodine recptor-2 (RyR2), sacroplasmic recticulum calcium-ATPase (SERCA), junction (Jun) and triadin (TRDN), were significantly lower in hiPSC than in hESCs.
Conclusions
The results indicate the calcium handling properties of hiPSC-derived cardiomyocytes are relatively immature to hESC counterparts.</description><identifier>ISSN: 1550-8943</identifier><identifier>ISSN: 2629-3269</identifier><identifier>EISSN: 1558-6804</identifier><identifier>EISSN: 2629-3277</identifier><identifier>DOI: 10.1007/s12015-011-9273-3</identifier><identifier>PMID: 21614516</identifier><language>eng</language><publisher>New York: Humana Press Inc</publisher><subject>Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Caffeine - pharmacology ; Calcium - metabolism ; Carrier Proteins - metabolism ; Cell Biology ; Cell Culture Techniques ; Cell Differentiation ; Cell Line ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - metabolism ; Homeostasis ; Humans ; Induced Pluripotent Stem Cells - cytology ; Life Sciences ; Muscle Proteins - metabolism ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - drug effects ; Myocytes, Cardiac - metabolism ; Regenerative Medicine/Tissue Engineering ; Reverse Transcriptase Polymerase Chain Reaction ; Ryanodine - pharmacology ; Ryanodine Receptor Calcium Release Channel - metabolism ; Sarcoplasmic Reticulum - metabolism ; Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism ; Stem Cells ; Time Factors</subject><ispartof>Stem cell reviews, 2011-11, Vol.7 (4), p.976-986</ispartof><rights>The Author(s) 2011</rights><rights>Springer Science+Business Media, LLC 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-6a3772c1abb557f3856a1a5139e0de425535048c091c2dd192ae7cf2ce29b3973</citedby><cites>FETCH-LOGICAL-c468t-6a3772c1abb557f3856a1a5139e0de425535048c091c2dd192ae7cf2ce29b3973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21614516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Yee-Ki</creatorcontrib><creatorcontrib>Ng, Kwong-Man</creatorcontrib><creatorcontrib>Lai, Wing-Hon</creatorcontrib><creatorcontrib>Chan, Yau-Chi</creatorcontrib><creatorcontrib>Lau, Yee-Man</creatorcontrib><creatorcontrib>Lian, Qizhou</creatorcontrib><creatorcontrib>Tse, Hung-Fat</creatorcontrib><creatorcontrib>Siu, Chung-Wah</creatorcontrib><title>Calcium Homeostasis in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes</title><title>Stem cell reviews</title><addtitle>Stem Cell Rev and Rep</addtitle><addtitle>Stem Cell Rev Rep</addtitle><description>Rationale
Cardiomyocytes generated from human induced pluripotent stem cells (hiPSCs) are suggested as the most promising candidate to replenish cardiomyocyte loss in regenerative medicine. Little is known about their calcium homeostasis, the key process underlying excitation-contraction coupling.
Objective
We investigated the calcium handling properties of hiPSC-derived cardiomyocytes and compared with those from human embryonic stem cells (hESCs).
Methods and Results
We differentiated cardiomyocytes from hiPSCs (IMR90 and KS1) and hESCs (H7 and HES3) with established protocols. Beating outgrowths from embryoid bodies were typically observed 2 weeks after induction. Cells in these outgrowths were stained positively for tropomyosin and sarcomeric alpha-actinin. Reverse-transcription polymerase chain reaction studies demonstrated the expressions of cardiac-specific markers in both hiPSC- and hESC-derived cardiomyocytes. Calcium handling properties of 20-day-old hiPSC- and hESC-derived cardiomyocytes were investigated using fluorescence confocal microscopy. Compared with hESC-derived cardiomyocytes, spontaneous calcium transients from both lines of hiPSC-derived cardiomyocytes were of significantly smaller amplitude and with slower maximal upstroke velocity. Better caffeine-induced calcium handling kinetics in hESC-CMs indicates a higher sacroplasmic recticulum calcium store. Furthermore, in contrast with hESC-derived cardiomyocytes, ryanodine did not reduce the amplitudes, maximal upstroke and decay velocity of calcium transients of hiPSC-derived cardiomyocytes. In addition, spatial inhomogeneity in temporal properties of calcium transients across the width of cardiomyocytes was more pronounced in hiPSC-derived cardiomyocytes than their hESC counterpart as revealed line-scan calcium imaging. Expressions of the key calcium-handling proteins including ryanodine recptor-2 (RyR2), sacroplasmic recticulum calcium-ATPase (SERCA), junction (Jun) and triadin (TRDN), were significantly lower in hiPSC than in hESCs.
Conclusions
The results indicate the calcium handling properties of hiPSC-derived cardiomyocytes are relatively immature to hESC counterparts.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Caffeine - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Biology</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Life Sciences</subject><subject>Muscle Proteins - metabolism</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - drug effects</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Ryanodine - pharmacology</subject><subject>Ryanodine Receptor Calcium Release Channel - metabolism</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism</subject><subject>Stem Cells</subject><subject>Time Factors</subject><issn>1550-8943</issn><issn>2629-3269</issn><issn>1558-6804</issn><issn>2629-3277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kd1rFDEUxYNY7Jd_gC8y-OJTbG6-ZvIiyNS6hUIr1ueQzdytKTOTNZkp7H_fbLfWVvApgfO7597DIeQdsE_AWH2SgTNQlAFQw2tBxStyAEo1VDdMvn74M9oYKfbJYc63jIlGNvCG7HPQIBXoA_K9db0P81At4oAxTy6HXIWxWsyDG6vzsZs9dtVVP6ewjhOOU_VjwqFqse_pKaZwV9TWpS7EYRP9ZsJ8TPZWrs_49vE9Ij_Pvl63C3px-e28_XJBvdTNRLUTdc09uOVSqXolGqUdOAXCIOtQcqWEYrLxzIDnXQeGO6z9invkZilMLY7I553vel4O2PlyW3K9XacwuLSx0QX7UhnDL3sT76zgXGujisHHR4MUf8-YJzuE7EswN2KcszWsBqk526768A95G-c0lnQF0lqKQhUIdpBPMeeEq6dTgNltXXZXly112W1dVpSZ988zPE386acAfAfkIo03mP5u_r_rPaFhoFY</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Lee, Yee-Ki</creator><creator>Ng, Kwong-Man</creator><creator>Lai, Wing-Hon</creator><creator>Chan, Yau-Chi</creator><creator>Lau, Yee-Man</creator><creator>Lian, Qizhou</creator><creator>Tse, Hung-Fat</creator><creator>Siu, Chung-Wah</creator><general>Humana Press Inc</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20111101</creationdate><title>Calcium Homeostasis in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes</title><author>Lee, Yee-Ki ; Ng, Kwong-Man ; Lai, Wing-Hon ; Chan, Yau-Chi ; Lau, Yee-Man ; Lian, Qizhou ; Tse, Hung-Fat ; Siu, Chung-Wah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-6a3772c1abb557f3856a1a5139e0de425535048c091c2dd192ae7cf2ce29b3973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Caffeine - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Biology</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - metabolism</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Induced Pluripotent Stem Cells - cytology</topic><topic>Life Sciences</topic><topic>Muscle Proteins - metabolism</topic><topic>Myocytes, Cardiac - cytology</topic><topic>Myocytes, Cardiac - drug effects</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Ryanodine - pharmacology</topic><topic>Ryanodine Receptor Calcium Release Channel - metabolism</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism</topic><topic>Stem Cells</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Yee-Ki</creatorcontrib><creatorcontrib>Ng, Kwong-Man</creatorcontrib><creatorcontrib>Lai, Wing-Hon</creatorcontrib><creatorcontrib>Chan, Yau-Chi</creatorcontrib><creatorcontrib>Lau, Yee-Man</creatorcontrib><creatorcontrib>Lian, Qizhou</creatorcontrib><creatorcontrib>Tse, Hung-Fat</creatorcontrib><creatorcontrib>Siu, Chung-Wah</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Stem cell reviews</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Yee-Ki</au><au>Ng, Kwong-Man</au><au>Lai, Wing-Hon</au><au>Chan, Yau-Chi</au><au>Lau, Yee-Man</au><au>Lian, Qizhou</au><au>Tse, Hung-Fat</au><au>Siu, Chung-Wah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium Homeostasis in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes</atitle><jtitle>Stem cell reviews</jtitle><stitle>Stem Cell Rev and Rep</stitle><addtitle>Stem Cell Rev Rep</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>7</volume><issue>4</issue><spage>976</spage><epage>986</epage><pages>976-986</pages><issn>1550-8943</issn><issn>2629-3269</issn><eissn>1558-6804</eissn><eissn>2629-3277</eissn><abstract>Rationale
Cardiomyocytes generated from human induced pluripotent stem cells (hiPSCs) are suggested as the most promising candidate to replenish cardiomyocyte loss in regenerative medicine. Little is known about their calcium homeostasis, the key process underlying excitation-contraction coupling.
Objective
We investigated the calcium handling properties of hiPSC-derived cardiomyocytes and compared with those from human embryonic stem cells (hESCs).
Methods and Results
We differentiated cardiomyocytes from hiPSCs (IMR90 and KS1) and hESCs (H7 and HES3) with established protocols. Beating outgrowths from embryoid bodies were typically observed 2 weeks after induction. Cells in these outgrowths were stained positively for tropomyosin and sarcomeric alpha-actinin. Reverse-transcription polymerase chain reaction studies demonstrated the expressions of cardiac-specific markers in both hiPSC- and hESC-derived cardiomyocytes. Calcium handling properties of 20-day-old hiPSC- and hESC-derived cardiomyocytes were investigated using fluorescence confocal microscopy. Compared with hESC-derived cardiomyocytes, spontaneous calcium transients from both lines of hiPSC-derived cardiomyocytes were of significantly smaller amplitude and with slower maximal upstroke velocity. Better caffeine-induced calcium handling kinetics in hESC-CMs indicates a higher sacroplasmic recticulum calcium store. Furthermore, in contrast with hESC-derived cardiomyocytes, ryanodine did not reduce the amplitudes, maximal upstroke and decay velocity of calcium transients of hiPSC-derived cardiomyocytes. In addition, spatial inhomogeneity in temporal properties of calcium transients across the width of cardiomyocytes was more pronounced in hiPSC-derived cardiomyocytes than their hESC counterpart as revealed line-scan calcium imaging. Expressions of the key calcium-handling proteins including ryanodine recptor-2 (RyR2), sacroplasmic recticulum calcium-ATPase (SERCA), junction (Jun) and triadin (TRDN), were significantly lower in hiPSC than in hESCs.
Conclusions
The results indicate the calcium handling properties of hiPSC-derived cardiomyocytes are relatively immature to hESC counterparts.</abstract><cop>New York</cop><pub>Humana Press Inc</pub><pmid>21614516</pmid><doi>10.1007/s12015-011-9273-3</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Stem cell reviews, 2011-11, Vol.7 (4), p.976-986 |
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| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Biomedical and Life Sciences Biomedical Engineering and Bioengineering Caffeine - pharmacology Calcium - metabolism Carrier Proteins - metabolism Cell Biology Cell Culture Techniques Cell Differentiation Cell Line Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Homeostasis Humans Induced Pluripotent Stem Cells - cytology Life Sciences Muscle Proteins - metabolism Myocytes, Cardiac - cytology Myocytes, Cardiac - drug effects Myocytes, Cardiac - metabolism Regenerative Medicine/Tissue Engineering Reverse Transcriptase Polymerase Chain Reaction Ryanodine - pharmacology Ryanodine Receptor Calcium Release Channel - metabolism Sarcoplasmic Reticulum - metabolism Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism Stem Cells Time Factors |
| title | Calcium Homeostasis in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes |
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