Candida glabrata strain relatedness by new microsatellite markers
We investigated six microsatellite markers to type 85 unrelated and 118 related isolates of Candida glabrata from 36 patients. Three new markers were selected from the complete sequence of CBS138 and three previously described markers, RPM2, MTI and ERG3 were used. We found a genetic diversity of 0....
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2012-01, Vol.31 (1), p.83-91 |
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| creator | Abbes, S. Sellami, H. Sellami, A. Hadrich, I. Amouri, I. Mahfoudh, N. Neji, S. Makni, F. Makni, H. Ayadi, A. |
| description | We investigated six microsatellite markers to type 85 unrelated and 118 related isolates of
Candida glabrata
from 36 patients. Three new markers were selected from the complete sequence of CBS138 and three previously described markers, RPM2, MTI and ERG3 were used. We found a genetic diversity of 0.949 by combining four of them. By applying the new microsatellite markers GLM4, GLM5 and GLM6 we were able to discriminate 29 isolates, originally identified by the more established markers, RPM2, MTI and ERG3. When epidemiologically closely related isolates from 36 patients were typed, 25 patients (72%) exhibited identical or highly related multilocus genotypes. We noted a microvariation in 4 of the patients. This minor change of one locus could be explained by a single step mutation. Since one of these patients had not received antifungal treatment; thus, the relationship between genome variation and antifungal therapy remains controversial. We can conclude from our analysis of these new microsatellite markers that they are highly selective and therefore should be considered as a useful typing system for differentiating related and unrelated isolates of
C. glabrata,
as well as being able to detect microvariation. |
| doi_str_mv | 10.1007/s10096-011-1280-4 |
| format | Article |
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Candida glabrata
from 36 patients. Three new markers were selected from the complete sequence of CBS138 and three previously described markers, RPM2, MTI and ERG3 were used. We found a genetic diversity of 0.949 by combining four of them. By applying the new microsatellite markers GLM4, GLM5 and GLM6 we were able to discriminate 29 isolates, originally identified by the more established markers, RPM2, MTI and ERG3. When epidemiologically closely related isolates from 36 patients were typed, 25 patients (72%) exhibited identical or highly related multilocus genotypes. We noted a microvariation in 4 of the patients. This minor change of one locus could be explained by a single step mutation. Since one of these patients had not received antifungal treatment; thus, the relationship between genome variation and antifungal therapy remains controversial. We can conclude from our analysis of these new microsatellite markers that they are highly selective and therefore should be considered as a useful typing system for differentiating related and unrelated isolates of
C. glabrata,
as well as being able to detect microvariation.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s10096-011-1280-4</identifier><identifier>PMID: 21598073</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Abscesses ; Antifungal Agents - pharmacology ; Base Sequence ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Candida glabrata ; Candida glabrata - classification ; Candida glabrata - drug effects ; Candida glabrata - genetics ; Candida glabrata - isolation & purification ; Candidiasis - drug therapy ; Candidiasis - microbiology ; Chromosomes ; DNA, Fungal - genetics ; Female ; Fluconazole - pharmacology ; Genetic diversity ; Genetic Markers ; Genetic Variation ; Genomes ; Genotype ; Genotypes ; Humans ; Infectious diseases ; Internal Medicine ; Male ; Medical Microbiology ; Medical sciences ; Microsatellite Repeats ; Microsatellites ; Mutation ; Mycological Typing Techniques ; Patients ; Polymorphism ; Sequence Analysis, DNA ; Vagina</subject><ispartof>European journal of clinical microbiology & infectious diseases, 2012-01, Vol.31 (1), p.83-91</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><rights>Springer-Verlag 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-af2dd28d023ea6c541b757a40997ec813a110a64f2377f0d3220b684e89ad1a13</citedby><cites>FETCH-LOGICAL-c466t-af2dd28d023ea6c541b757a40997ec813a110a64f2377f0d3220b684e89ad1a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10096-011-1280-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10096-011-1280-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25595215$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21598073$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abbes, S.</creatorcontrib><creatorcontrib>Sellami, H.</creatorcontrib><creatorcontrib>Sellami, A.</creatorcontrib><creatorcontrib>Hadrich, I.</creatorcontrib><creatorcontrib>Amouri, I.</creatorcontrib><creatorcontrib>Mahfoudh, N.</creatorcontrib><creatorcontrib>Neji, S.</creatorcontrib><creatorcontrib>Makni, F.</creatorcontrib><creatorcontrib>Makni, H.</creatorcontrib><creatorcontrib>Ayadi, A.</creatorcontrib><title>Candida glabrata strain relatedness by new microsatellite markers</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>We investigated six microsatellite markers to type 85 unrelated and 118 related isolates of
Candida glabrata
from 36 patients. Three new markers were selected from the complete sequence of CBS138 and three previously described markers, RPM2, MTI and ERG3 were used. We found a genetic diversity of 0.949 by combining four of them. By applying the new microsatellite markers GLM4, GLM5 and GLM6 we were able to discriminate 29 isolates, originally identified by the more established markers, RPM2, MTI and ERG3. When epidemiologically closely related isolates from 36 patients were typed, 25 patients (72%) exhibited identical or highly related multilocus genotypes. We noted a microvariation in 4 of the patients. This minor change of one locus could be explained by a single step mutation. Since one of these patients had not received antifungal treatment; thus, the relationship between genome variation and antifungal therapy remains controversial. We can conclude from our analysis of these new microsatellite markers that they are highly selective and therefore should be considered as a useful typing system for differentiating related and unrelated isolates of
C. glabrata,
as well as being able to detect microvariation.</description><subject>Abscesses</subject><subject>Antifungal Agents - pharmacology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Candida glabrata</subject><subject>Candida glabrata - classification</subject><subject>Candida glabrata - drug effects</subject><subject>Candida glabrata - genetics</subject><subject>Candida glabrata - isolation & purification</subject><subject>Candidiasis - drug therapy</subject><subject>Candidiasis - microbiology</subject><subject>Chromosomes</subject><subject>DNA, Fungal - genetics</subject><subject>Female</subject><subject>Fluconazole - pharmacology</subject><subject>Genetic diversity</subject><subject>Genetic Markers</subject><subject>Genetic Variation</subject><subject>Genomes</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>Medical Microbiology</subject><subject>Medical sciences</subject><subject>Microsatellite Repeats</subject><subject>Microsatellites</subject><subject>Mutation</subject><subject>Mycological Typing Techniques</subject><subject>Patients</subject><subject>Polymorphism</subject><subject>Sequence Analysis, DNA</subject><subject>Vagina</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1LAzEQhoMotlZ_gBdZBNHLaj43ybEUv6DgRc_L7CZbtm63Ndki_fdO2WpB0EsCyTPvzDsvIeeM3jJK9V3E02YpZSxl3NBUHpAhk0KlUmhxSIbUCplazcWAnMQ4p1hjtD4mA86UNVSLIRlPoHW1g2TWQBGggyR2Aeo2Cb6BzrvWx5gUm6T1n8miLsMy4mvT1J1PFhDefYin5KiCJvqz3T0ibw_3r5OndPry-DwZT9NSZlmXQsWd48ZRLjxkpZKs0EqDpNZqXxomgDEKmay40LqiTnBOi8xIbyw4BkyMyHWvuwrLj7WPXb6oY4mzQOuX65hbmjElqZFI3vxLMlwYp6htEL38hc6X69CiD9RTNtMKtzQirIe29mPwVb4KNbrfoFK-DSLvg8hRN98GkW9nuNgJr4uFdz8V35tH4GoHQCyhqQK0ZR33nFJWIYwc77mIX-3Mh_2Ef3f_AjEFnfY</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Abbes, S.</creator><creator>Sellami, H.</creator><creator>Sellami, A.</creator><creator>Hadrich, I.</creator><creator>Amouri, I.</creator><creator>Mahfoudh, N.</creator><creator>Neji, S.</creator><creator>Makni, F.</creator><creator>Makni, H.</creator><creator>Ayadi, A.</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20120101</creationdate><title>Candida glabrata strain relatedness by new microsatellite markers</title><author>Abbes, S. ; Sellami, H. ; Sellami, A. ; Hadrich, I. ; Amouri, I. ; Mahfoudh, N. ; Neji, S. ; Makni, F. ; Makni, H. ; Ayadi, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-af2dd28d023ea6c541b757a40997ec813a110a64f2377f0d3220b684e89ad1a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Abscesses</topic><topic>Antifungal Agents - 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Academic</collection><jtitle>European journal of clinical microbiology & infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abbes, S.</au><au>Sellami, H.</au><au>Sellami, A.</au><au>Hadrich, I.</au><au>Amouri, I.</au><au>Mahfoudh, N.</au><au>Neji, S.</au><au>Makni, F.</au><au>Makni, H.</au><au>Ayadi, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Candida glabrata strain relatedness by new microsatellite markers</atitle><jtitle>European journal of clinical microbiology & infectious diseases</jtitle><stitle>Eur J Clin Microbiol Infect Dis</stitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>31</volume><issue>1</issue><spage>83</spage><epage>91</epage><pages>83-91</pages><issn>0934-9723</issn><eissn>1435-4373</eissn><abstract>We investigated six microsatellite markers to type 85 unrelated and 118 related isolates of
Candida glabrata
from 36 patients. Three new markers were selected from the complete sequence of CBS138 and three previously described markers, RPM2, MTI and ERG3 were used. We found a genetic diversity of 0.949 by combining four of them. By applying the new microsatellite markers GLM4, GLM5 and GLM6 we were able to discriminate 29 isolates, originally identified by the more established markers, RPM2, MTI and ERG3. When epidemiologically closely related isolates from 36 patients were typed, 25 patients (72%) exhibited identical or highly related multilocus genotypes. We noted a microvariation in 4 of the patients. This minor change of one locus could be explained by a single step mutation. Since one of these patients had not received antifungal treatment; thus, the relationship between genome variation and antifungal therapy remains controversial. We can conclude from our analysis of these new microsatellite markers that they are highly selective and therefore should be considered as a useful typing system for differentiating related and unrelated isolates of
C. glabrata,
as well as being able to detect microvariation.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21598073</pmid><doi>10.1007/s10096-011-1280-4</doi><tpages>9</tpages></addata></record> |
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| subjects | Abscesses Antifungal Agents - pharmacology Base Sequence Biological and medical sciences Biomedical and Life Sciences Biomedicine Candida glabrata Candida glabrata - classification Candida glabrata - drug effects Candida glabrata - genetics Candida glabrata - isolation & purification Candidiasis - drug therapy Candidiasis - microbiology Chromosomes DNA, Fungal - genetics Female Fluconazole - pharmacology Genetic diversity Genetic Markers Genetic Variation Genomes Genotype Genotypes Humans Infectious diseases Internal Medicine Male Medical Microbiology Medical sciences Microsatellite Repeats Microsatellites Mutation Mycological Typing Techniques Patients Polymorphism Sequence Analysis, DNA Vagina |
| title | Candida glabrata strain relatedness by new microsatellite markers |
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