A simple fluorescent labeling technique to study virus adsorption in Newcastle disease virus infected cells

► This study demonstrates PKH67 and PKH26 labeling of enveloped NDV particles. ► Specificity of labeling was determined by three different techniques. ► Infectivity of the virus particles are not altered significantly by the labeling. ► The technique is fast, simple, convenient and easy to perform....

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Veröffentlicht in:	Enzyme and microbial technology 2011-08, Vol.49 (3), p.255-259
Hauptverfasser:	Balogh, András, Pap, Marianna, Markó, Lajos, Koloszár, Ibolya, Csatáry, László K., Szeberényi, József
Format:	Artikel
Sprache:	eng
Schlagworte:	3D imaging adenosine triphosphate Adsorption Animals Apoptosis Biological and medical sciences Biotechnology Blotting, Western Cell Line, Tumor - virology Cell Membrane - ultrastructure Cell Membrane - virology confocal laser scanning microscopy Confocal microscopy Cytopathogenic Effect, Viral Cytoplasm - virology cytotoxicity Detergents - pharmacology dyes Endocytosis Enveloped virus fluorescence Fluorescent Antibody Technique, Direct Fluorescent Dyes - analysis Fluorescent labeling Fundamental and applied biological sciences. Psychology Membrane Fusion Microscopy, Confocal Newcastle disease virus Newcastle disease virus - drug effects Newcastle disease virus - physiology Organic Chemicals - analysis Paramyxoviridae pathogenicity Pheochromocytoma - pathology PKH dyes quantitative polymerase chain reaction Rats RNA RNA, Viral - analysis Staining and Labeling - methods virion Virus Internalization viruses Western blotting
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