Decolorization of indigo carmine by laccase displayed on Bacillus subtilis spores

Blue multicopper oxidases, laccases displayed on the surface of Bacillus spores were used to decolorize a widely used textile dyestuff, indigo carmine. The laccase-encoding gene of Bacillus subtilis, cotA, was cloned and expressed in B. subtilis DB104, and the expressed enzyme was spontaneously loca...

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Veröffentlicht in:	Enzyme and microbial technology 2011-06, Vol.49 (1), p.100-104
Hauptverfasser:	Cho, Eun-Ah, Seo, Jiyoung, Lee, Dong-Woo, Pan, Jae-Gu
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetonitrile Bacillus Bacillus subtilis Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Benzothiazoles - metabolism Biodegradation, Environmental Biological and medical sciences Bioreactors - microbiology Biotechnology Coloring Agents - metabolism Decolorization DNA, Bacterial - genetics Effluents Enzyme Stability Enzymes Enzymes, Immobilized - genetics Enzymes, Immobilized - metabolism fabrics Fundamental and applied biological sciences. Psychology genes Genes, Bacterial hexane Indigo Indigo carmine Indigo Carmine - metabolism Kinetics Laccase Laccase - genetics Laccase - metabolism multicopper oxidase n-Hexane Oxidation pH effects pretreatment Recombinant Proteins - genetics Recombinant Proteins - metabolism Recycling Solvents Spore display Spores Spores, Bacterial - enzymology Substrate Specificity Sulfonic Acids - metabolism Textile Industry Textiles Waste Disposal, Fluid - methods
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