Effect of sire breed and genetic merit for carcass weight on the transcriptional regulation of the somatotropic axis in longissimus dorsi of crossbred steers
The somatotropic axis plays an important role in postnatal growth, development, and differentiation of skeletal muscle. The aim of this study was to examine the effect of sire breed and sire EPD for carcass weight (EPDcwt) on the expression of components of the somatotropic axis in LM of beef cattle...
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| description | The somatotropic axis plays an important role in postnatal growth, development, and differentiation of skeletal muscle. The aim of this study was to examine the effect of sire breed and sire EPD for carcass weight (EPDcwt) on the expression of components of the somatotropic axis in LM of beef cattle at slaughter. Crossbred Aberdeen Angus (AA; n = 17) and Belgian Blue (BB; n = 16) steers born to Holstein-Friesian dams and sired by bulls with either high (H) or low (L) EPDcwt were used in the study. Thus, there were 4 genetic groups [i.e., BBH (n = 8), BBL (n = 8), AAH (n = 8), and AAL (n = 9)]. Blood samples were collected via jugular venipuncture at regular intervals for analysis of plasma concentrations of IGF-1 and insulin. Total RNA was isolated from LM collected at slaughter, and the mRNA expression of IGF-1, IGF-2, their receptors (IGF-1R; IGF-2R), 6 IGFBP, acid labile subunit (ALS), and GH receptor (GHR) was measured by real-time reverse-transcription quantitative PCR. There was no effect of either sire breed or EPDcwt on concentrations of circulating IGF or insulin (P > 0.05). Gene expression of IGF-1R and IGFBP3 was upregulated in AA (P < 0.001) compared with BB, whereas IGF-1 was upregulated in H compared with L animals (P < 0.01). Correlation analysis indicated moderate positive associations between gene expression of IGFBP3 and IGF-1 (r = 0.54; P < 0.001) and IGF-1R (r = 0.48; P < 0.01). In addition, correlation analysis revealed that mRNA expression of IGFBP3 was moderately negatively associated with LM area per kilogram of carcass weight (r = –0.40; P < 0.05). Greater gene expression of IGF-1 and reduced transcript abundance of IGFBP3 in muscle may have a role in increased muscle growth potential in steers during the finishing period. These data will contribute to a better understanding of the molecular control of muscle growth at a tissue level in cattle. |
| doi_str_mv | 10.2527/jas.2011-4032 |
| format | Article |
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M ; Kenny, D. A ; Keane, M. G ; Waters, S. M</creator><creatorcontrib>Keady, S. M ; Kenny, D. A ; Keane, M. G ; Waters, S. M</creatorcontrib><description>The somatotropic axis plays an important role in postnatal growth, development, and differentiation of skeletal muscle. The aim of this study was to examine the effect of sire breed and sire EPD for carcass weight (EPDcwt) on the expression of components of the somatotropic axis in LM of beef cattle at slaughter. Crossbred Aberdeen Angus (AA; n = 17) and Belgian Blue (BB; n = 16) steers born to Holstein-Friesian dams and sired by bulls with either high (H) or low (L) EPDcwt were used in the study. Thus, there were 4 genetic groups [i.e., BBH (n = 8), BBL (n = 8), AAH (n = 8), and AAL (n = 9)]. Blood samples were collected via jugular venipuncture at regular intervals for analysis of plasma concentrations of IGF-1 and insulin. Total RNA was isolated from LM collected at slaughter, and the mRNA expression of IGF-1, IGF-2, their receptors (IGF-1R; IGF-2R), 6 IGFBP, acid labile subunit (ALS), and GH receptor (GHR) was measured by real-time reverse-transcription quantitative PCR. There was no effect of either sire breed or EPDcwt on concentrations of circulating IGF or insulin (P > 0.05). Gene expression of IGF-1R and IGFBP3 was upregulated in AA (P < 0.001) compared with BB, whereas IGF-1 was upregulated in H compared with L animals (P < 0.01). Correlation analysis indicated moderate positive associations between gene expression of IGFBP3 and IGF-1 (r = 0.54; P < 0.001) and IGF-1R (r = 0.48; P < 0.01). In addition, correlation analysis revealed that mRNA expression of IGFBP3 was moderately negatively associated with LM area per kilogram of carcass weight (r = –0.40; P < 0.05). Greater gene expression of IGF-1 and reduced transcript abundance of IGFBP3 in muscle may have a role in increased muscle growth potential in steers during the finishing period. These data will contribute to a better understanding of the molecular control of muscle growth at a tissue level in cattle.</description><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><identifier>DOI: 10.2527/jas.2011-4032</identifier><identifier>PMID: 21724946</identifier><language>eng</language><publisher>Champaign, IL: American Society of Animal Science</publisher><subject>Abundance ; Actins - genetics ; Actins - metabolism ; Amyotrophic lateral sclerosis ; Angus ; Animal productions ; Animals ; Beef ; beef cattle ; Belgian Blue ; Biological and medical sciences ; blood ; Body Weight - genetics ; bulls ; carcass weight ; Carcasses ; Cattle - genetics ; Cattle - physiology ; Correlation analysis ; Data processing ; Differentiation ; Food industries ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation - physiology ; Gene regulation ; genetic merit ; Growth hormone ; Holstein ; Hydroxymethylbilane Synthase - genetics ; Hydroxymethylbilane Synthase - metabolism ; Insulin ; Insulin - blood ; insulin-like growth factor binding proteins ; Insulin-like growth factor I ; insulin-like growth factor II ; Insulin-like growth factor-binding protein 3 ; Insulin-like growth factors ; longissimus muscle ; Male ; Meat and meat product industries ; messenger RNA ; Muscle, Skeletal - metabolism ; Muscles ; Peptide Elongation Factor 1 - genetics ; Peptide Elongation Factor 1 - metabolism ; Polymerase chain reaction ; Receptors, Somatotropin ; reverse transcription ; sires ; Skeletal muscle ; Slaughter ; Somatomedins - genetics ; Somatomedins - metabolism ; steers ; Terrestrial animal productions ; Transcription ; transcription (genetics) ; Vertebrates</subject><ispartof>Journal of animal science, 2011-12, Vol.89 (12), p.4007-4016</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25261972$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21724946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Keady, S. M</creatorcontrib><creatorcontrib>Kenny, D. A</creatorcontrib><creatorcontrib>Keane, M. G</creatorcontrib><creatorcontrib>Waters, S. M</creatorcontrib><title>Effect of sire breed and genetic merit for carcass weight on the transcriptional regulation of the somatotropic axis in longissimus dorsi of crossbred steers</title><title>Journal of animal science</title><addtitle>J Anim Sci</addtitle><description>The somatotropic axis plays an important role in postnatal growth, development, and differentiation of skeletal muscle. The aim of this study was to examine the effect of sire breed and sire EPD for carcass weight (EPDcwt) on the expression of components of the somatotropic axis in LM of beef cattle at slaughter. Crossbred Aberdeen Angus (AA; n = 17) and Belgian Blue (BB; n = 16) steers born to Holstein-Friesian dams and sired by bulls with either high (H) or low (L) EPDcwt were used in the study. Thus, there were 4 genetic groups [i.e., BBH (n = 8), BBL (n = 8), AAH (n = 8), and AAL (n = 9)]. Blood samples were collected via jugular venipuncture at regular intervals for analysis of plasma concentrations of IGF-1 and insulin. Total RNA was isolated from LM collected at slaughter, and the mRNA expression of IGF-1, IGF-2, their receptors (IGF-1R; IGF-2R), 6 IGFBP, acid labile subunit (ALS), and GH receptor (GHR) was measured by real-time reverse-transcription quantitative PCR. There was no effect of either sire breed or EPDcwt on concentrations of circulating IGF or insulin (P > 0.05). Gene expression of IGF-1R and IGFBP3 was upregulated in AA (P < 0.001) compared with BB, whereas IGF-1 was upregulated in H compared with L animals (P < 0.01). Correlation analysis indicated moderate positive associations between gene expression of IGFBP3 and IGF-1 (r = 0.54; P < 0.001) and IGF-1R (r = 0.48; P < 0.01). In addition, correlation analysis revealed that mRNA expression of IGFBP3 was moderately negatively associated with LM area per kilogram of carcass weight (r = –0.40; P < 0.05). Greater gene expression of IGF-1 and reduced transcript abundance of IGFBP3 in muscle may have a role in increased muscle growth potential in steers during the finishing period. These data will contribute to a better understanding of the molecular control of muscle growth at a tissue level in cattle.</description><subject>Abundance</subject><subject>Actins - genetics</subject><subject>Actins - metabolism</subject><subject>Amyotrophic lateral sclerosis</subject><subject>Angus</subject><subject>Animal productions</subject><subject>Animals</subject><subject>Beef</subject><subject>beef cattle</subject><subject>Belgian Blue</subject><subject>Biological and medical sciences</subject><subject>blood</subject><subject>Body Weight - genetics</subject><subject>bulls</subject><subject>carcass weight</subject><subject>Carcasses</subject><subject>Cattle - genetics</subject><subject>Cattle - physiology</subject><subject>Correlation analysis</subject><subject>Data processing</subject><subject>Differentiation</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - physiology</subject><subject>Gene regulation</subject><subject>genetic merit</subject><subject>Growth hormone</subject><subject>Holstein</subject><subject>Hydroxymethylbilane Synthase - genetics</subject><subject>Hydroxymethylbilane Synthase - metabolism</subject><subject>Insulin</subject><subject>Insulin - blood</subject><subject>insulin-like growth factor binding proteins</subject><subject>Insulin-like growth factor I</subject><subject>insulin-like growth factor II</subject><subject>Insulin-like growth factor-binding protein 3</subject><subject>Insulin-like growth factors</subject><subject>longissimus muscle</subject><subject>Male</subject><subject>Meat and meat product industries</subject><subject>messenger RNA</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscles</subject><subject>Peptide Elongation Factor 1 - genetics</subject><subject>Peptide Elongation Factor 1 - metabolism</subject><subject>Polymerase chain reaction</subject><subject>Receptors, Somatotropin</subject><subject>reverse transcription</subject><subject>sires</subject><subject>Skeletal muscle</subject><subject>Slaughter</subject><subject>Somatomedins - genetics</subject><subject>Somatomedins - metabolism</subject><subject>steers</subject><subject>Terrestrial animal productions</subject><subject>Transcription</subject><subject>transcription (genetics)</subject><subject>Vertebrates</subject><issn>0021-8812</issn><issn>1525-3163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90U1v1DAQBmALgehSOHIFXxBcUmzHdtbHqiofUiUO0HM0ccZbV0m8eBwBP4b_iqMu4sZcrJEev7JeM_ZSigtlVPf-HuhCCSkbLVr1iO2kUaZppW0fs50QSjb7vVRn7BnRvRBSGWeesjMlO6Wdtjv2-zoE9IWnwClm5ENGHDksIz_ggiV6PmOOhYeUuYfsgYj_wHi4q1cWXu6QlwwL-RyPJaYFJp7xsE6wLVvoJijNUFLJ6Vjj4GckHhc-peUQieK8Eh9TprhpnxNRfcLIqSBmes6eBJgIX5zOc3b74frb1afm5svHz1eXN01QriuNtADDHp1BY41Wo2nD0IpWj2EcnMBxL60Bo4UeArZgglUaOq8NhkFKCKE9Z28fco85fV-RSj9H8jhNsGBaqXfCOKuVdVW--6-UQnbOSu02-upE12HGsT_mOEP-1f8tv4I3JwDkYQq1SB_pnzPKStep6l4_uACph0Ou5vZr_XMt6jjbde0fIPygCg</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Keady, S. M</creator><creator>Kenny, D. A</creator><creator>Keane, M. G</creator><creator>Waters, S. M</creator><general>American Society of Animal Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20111201</creationdate><title>Effect of sire breed and genetic merit for carcass weight on the transcriptional regulation of the somatotropic axis in longissimus dorsi of crossbred steers</title><author>Keady, S. M ; Kenny, D. A ; Keane, M. G ; Waters, S. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f297t-16aab8e95e56542d53fb3034dfdb90ed8165a5404bfe3a5f624a7c45efb11aff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Abundance</topic><topic>Actins - genetics</topic><topic>Actins - metabolism</topic><topic>Amyotrophic lateral sclerosis</topic><topic>Angus</topic><topic>Animal productions</topic><topic>Animals</topic><topic>Beef</topic><topic>beef cattle</topic><topic>Belgian Blue</topic><topic>Biological and medical sciences</topic><topic>blood</topic><topic>Body Weight - genetics</topic><topic>bulls</topic><topic>carcass weight</topic><topic>Carcasses</topic><topic>Cattle - genetics</topic><topic>Cattle - physiology</topic><topic>Correlation analysis</topic><topic>Data processing</topic><topic>Differentiation</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - physiology</topic><topic>Gene regulation</topic><topic>genetic merit</topic><topic>Growth hormone</topic><topic>Holstein</topic><topic>Hydroxymethylbilane Synthase - genetics</topic><topic>Hydroxymethylbilane Synthase - metabolism</topic><topic>Insulin</topic><topic>Insulin - blood</topic><topic>insulin-like growth factor binding proteins</topic><topic>Insulin-like growth factor I</topic><topic>insulin-like growth factor II</topic><topic>Insulin-like growth factor-binding protein 3</topic><topic>Insulin-like growth factors</topic><topic>longissimus muscle</topic><topic>Male</topic><topic>Meat and meat product industries</topic><topic>messenger RNA</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscles</topic><topic>Peptide Elongation Factor 1 - genetics</topic><topic>Peptide Elongation Factor 1 - metabolism</topic><topic>Polymerase chain reaction</topic><topic>Receptors, Somatotropin</topic><topic>reverse transcription</topic><topic>sires</topic><topic>Skeletal muscle</topic><topic>Slaughter</topic><topic>Somatomedins - genetics</topic><topic>Somatomedins - metabolism</topic><topic>steers</topic><topic>Terrestrial animal productions</topic><topic>Transcription</topic><topic>transcription (genetics)</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Keady, S. M</creatorcontrib><creatorcontrib>Kenny, D. A</creatorcontrib><creatorcontrib>Keane, M. G</creatorcontrib><creatorcontrib>Waters, S. M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keady, S. M</au><au>Kenny, D. A</au><au>Keane, M. G</au><au>Waters, S. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of sire breed and genetic merit for carcass weight on the transcriptional regulation of the somatotropic axis in longissimus dorsi of crossbred steers</atitle><jtitle>Journal of animal science</jtitle><addtitle>J Anim Sci</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>89</volume><issue>12</issue><spage>4007</spage><epage>4016</epage><pages>4007-4016</pages><issn>0021-8812</issn><eissn>1525-3163</eissn><abstract>The somatotropic axis plays an important role in postnatal growth, development, and differentiation of skeletal muscle. The aim of this study was to examine the effect of sire breed and sire EPD for carcass weight (EPDcwt) on the expression of components of the somatotropic axis in LM of beef cattle at slaughter. Crossbred Aberdeen Angus (AA; n = 17) and Belgian Blue (BB; n = 16) steers born to Holstein-Friesian dams and sired by bulls with either high (H) or low (L) EPDcwt were used in the study. Thus, there were 4 genetic groups [i.e., BBH (n = 8), BBL (n = 8), AAH (n = 8), and AAL (n = 9)]. Blood samples were collected via jugular venipuncture at regular intervals for analysis of plasma concentrations of IGF-1 and insulin. Total RNA was isolated from LM collected at slaughter, and the mRNA expression of IGF-1, IGF-2, their receptors (IGF-1R; IGF-2R), 6 IGFBP, acid labile subunit (ALS), and GH receptor (GHR) was measured by real-time reverse-transcription quantitative PCR. There was no effect of either sire breed or EPDcwt on concentrations of circulating IGF or insulin (P > 0.05). Gene expression of IGF-1R and IGFBP3 was upregulated in AA (P < 0.001) compared with BB, whereas IGF-1 was upregulated in H compared with L animals (P < 0.01). Correlation analysis indicated moderate positive associations between gene expression of IGFBP3 and IGF-1 (r = 0.54; P < 0.001) and IGF-1R (r = 0.48; P < 0.01). In addition, correlation analysis revealed that mRNA expression of IGFBP3 was moderately negatively associated with LM area per kilogram of carcass weight (r = –0.40; P < 0.05). Greater gene expression of IGF-1 and reduced transcript abundance of IGFBP3 in muscle may have a role in increased muscle growth potential in steers during the finishing period. These data will contribute to a better understanding of the molecular control of muscle growth at a tissue level in cattle.</abstract><cop>Champaign, IL</cop><pub>American Society of Animal Science</pub><pmid>21724946</pmid><doi>10.2527/jas.2011-4032</doi><tpages>10</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Abundance Actins - genetics Actins - metabolism Amyotrophic lateral sclerosis Angus Animal productions Animals Beef beef cattle Belgian Blue Biological and medical sciences blood Body Weight - genetics bulls carcass weight Carcasses Cattle - genetics Cattle - physiology Correlation analysis Data processing Differentiation Food industries Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation - physiology Gene regulation genetic merit Growth hormone Holstein Hydroxymethylbilane Synthase - genetics Hydroxymethylbilane Synthase - metabolism Insulin Insulin - blood insulin-like growth factor binding proteins Insulin-like growth factor I insulin-like growth factor II Insulin-like growth factor-binding protein 3 Insulin-like growth factors longissimus muscle Male Meat and meat product industries messenger RNA Muscle, Skeletal - metabolism Muscles Peptide Elongation Factor 1 - genetics Peptide Elongation Factor 1 - metabolism Polymerase chain reaction Receptors, Somatotropin reverse transcription sires Skeletal muscle Slaughter Somatomedins - genetics Somatomedins - metabolism steers Terrestrial animal productions Transcription transcription (genetics) Vertebrates |
| title | Effect of sire breed and genetic merit for carcass weight on the transcriptional regulation of the somatotropic axis in longissimus dorsi of crossbred steers |
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