Enhanced Immune Response Against Pertussis Toxoid by IgA-Loaded Chitosan–Dextran Sulfate Nanoparticles
The objective of the present study was to evaluate immunological activities of chitosan–dextran sulfate (CS–DS) nanoparticle formulation of pertussis toxoid (PTXd) and its combination with a potential immunological adjuvant, immunoglobulin A (IgA). CS–DS nanoparticles were prepared using a complex c...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 2012-01, Vol.101 (1), p.233-244 |
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| creator | Sharma, Sameer Mukkur, Trilochan K.S. Benson, Heather A.E. Chen, Yan |
| description | The objective of the present study was to evaluate immunological activities of chitosan–dextran sulfate (CS–DS) nanoparticle formulation of pertussis toxoid (PTXd) and its combination with a potential immunological adjuvant, immunoglobulin A (IgA). CS–DS nanoparticles were prepared using a complex coacervation (polyelectrolyte complexation) technique. CS–DS nanoparticle formulations with size and zeta potential in a range of 300–350nm and +40–+55mV, respectively, were obtained. An entrapment efficiency of more than 90% was obtained for pertussis toxin and IgA in CS–DS nanoparticles. All loaded nanoparticle formulations showed less than 20% of release within 24h in in vitro release studies. The immunological evaluation of developed formulations in female Balb/c mice groups showed that the CS–DS nanoparticles formulations induced significantly higher serum IgG and IgG1 titers (p < 0.05) as compared with conventional alum-adjuvanted PTXd formulation administered by subcutaneous route. This study indicated the potential of CS–DS nanoparticles to be a simple and effective particulate delivery system with in-built immunological adjuvant property for acellular protein antigens. The study also revealed the potential important role of IgA-loaded CS–DS nanoparticles as a novel immunological adjuvant for vaccine delivery. © 2011 Wiley Periodicals, Inc. and the American Pharmacists Association. |
| doi_str_mv | 10.1002/jps.22763 |
| format | Article |
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CS–DS nanoparticles were prepared using a complex coacervation (polyelectrolyte complexation) technique. CS–DS nanoparticle formulations with size and zeta potential in a range of 300–350nm and +40–+55mV, respectively, were obtained. An entrapment efficiency of more than 90% was obtained for pertussis toxin and IgA in CS–DS nanoparticles. All loaded nanoparticle formulations showed less than 20% of release within 24h in in vitro release studies. The immunological evaluation of developed formulations in female Balb/c mice groups showed that the CS–DS nanoparticles formulations induced significantly higher serum IgG and IgG1 titers (p < 0.05) as compared with conventional alum-adjuvanted PTXd formulation administered by subcutaneous route. This study indicated the potential of CS–DS nanoparticles to be a simple and effective particulate delivery system with in-built immunological adjuvant property for acellular protein antigens. The study also revealed the potential important role of IgA-loaded CS–DS nanoparticles as a novel immunological adjuvant for vaccine delivery. © 2011 Wiley Periodicals, Inc. and the American Pharmacists Association.</description><identifier>ISSN: 0022-3549</identifier><identifier>ISSN: 1520-6017</identifier><identifier>EISSN: 1520-6017</identifier><identifier>DOI: 10.1002/jps.22763</identifier><identifier>PMID: 21953499</identifier><identifier>CODEN: JPMSAE</identifier><language>eng</language><publisher>Hoboken: Elsevier Inc</publisher><subject><![CDATA[Adjuvants ; Adjuvants, Immunologic - administration & dosage ; Adjuvants, Immunologic - chemistry ; Alum Compounds - administration & dosage ; Alum Compounds - chemistry ; Animals ; Biological and medical sciences ; Chemistry, Pharmaceutical - methods ; chitosan ; Chitosan - administration & dosage ; Chitosan - chemistry ; Chitosan - immunology ; Coacervation ; dextran sulfate ; Dextran Sulfate - administration & dosage ; Dextran Sulfate - chemistry ; Dextran Sulfate - immunology ; Drug Carriers - administration & dosage ; Drug Carriers - chemistry ; Drug Delivery Systems - methods ; Female ; General pharmacology ; Immune response ; Immunoglobulin A ; Immunoglobulin A - administration & dosage ; Immunoglobulin A - chemistry ; Immunoglobulin A - immunology ; Immunoglobulin G ; Immunoglobulin G - blood ; Medical sciences ; Mice ; Mice, Inbred BALB C ; nanoparticles ; Nanoparticles - administration & dosage ; Nanoparticles - chemistry ; Pertussis ; pertussis toxin ; Pharmaceutical technology. 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Drug treatments ; Polyelectrolytes ; proteins ; Sulfate ; Toxoids ; Toxoids - antagonists & inhibitors ; Toxoids - immunology ; vaccine adjuvants ; vaccine delivery ; Vaccines ; Zeta potential]]></subject><ispartof>Journal of pharmaceutical sciences, 2012-01, Vol.101 (1), p.233-244</ispartof><rights>2012 Wiley-Liss, Inc.</rights><rights>Copyright © 2011 Wiley‐Liss, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4973-818b4b62b6f930e4ce30a21f2dcb8ca1b69d5101d873442792b1e66bf2784fa63</citedby><cites>FETCH-LOGICAL-c4973-818b4b62b6f930e4ce30a21f2dcb8ca1b69d5101d873442792b1e66bf2784fa63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjps.22763$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjps.22763$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25505708$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21953499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sharma, Sameer</creatorcontrib><creatorcontrib>Mukkur, Trilochan K.S.</creatorcontrib><creatorcontrib>Benson, Heather A.E.</creatorcontrib><creatorcontrib>Chen, Yan</creatorcontrib><title>Enhanced Immune Response Against Pertussis Toxoid by IgA-Loaded Chitosan–Dextran Sulfate Nanoparticles</title><title>Journal of pharmaceutical sciences</title><addtitle>J. Pharm. Sci</addtitle><description>The objective of the present study was to evaluate immunological activities of chitosan–dextran sulfate (CS–DS) nanoparticle formulation of pertussis toxoid (PTXd) and its combination with a potential immunological adjuvant, immunoglobulin A (IgA). CS–DS nanoparticles were prepared using a complex coacervation (polyelectrolyte complexation) technique. CS–DS nanoparticle formulations with size and zeta potential in a range of 300–350nm and +40–+55mV, respectively, were obtained. An entrapment efficiency of more than 90% was obtained for pertussis toxin and IgA in CS–DS nanoparticles. All loaded nanoparticle formulations showed less than 20% of release within 24h in in vitro release studies. The immunological evaluation of developed formulations in female Balb/c mice groups showed that the CS–DS nanoparticles formulations induced significantly higher serum IgG and IgG1 titers (p < 0.05) as compared with conventional alum-adjuvanted PTXd formulation administered by subcutaneous route. This study indicated the potential of CS–DS nanoparticles to be a simple and effective particulate delivery system with in-built immunological adjuvant property for acellular protein antigens. The study also revealed the potential important role of IgA-loaded CS–DS nanoparticles as a novel immunological adjuvant for vaccine delivery. © 2011 Wiley Periodicals, Inc. and the American Pharmacists Association.</description><subject>Adjuvants</subject><subject>Adjuvants, Immunologic - administration & dosage</subject><subject>Adjuvants, Immunologic - chemistry</subject><subject>Alum Compounds - administration & dosage</subject><subject>Alum Compounds - chemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chemistry, Pharmaceutical - methods</subject><subject>chitosan</subject><subject>Chitosan - administration & dosage</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - immunology</subject><subject>Coacervation</subject><subject>dextran sulfate</subject><subject>Dextran Sulfate - administration & dosage</subject><subject>Dextran Sulfate - chemistry</subject><subject>Dextran Sulfate - immunology</subject><subject>Drug Carriers - administration & dosage</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Delivery Systems - methods</subject><subject>Female</subject><subject>General pharmacology</subject><subject>Immune response</subject><subject>Immunoglobulin A</subject><subject>Immunoglobulin A - administration & dosage</subject><subject>Immunoglobulin A - chemistry</subject><subject>Immunoglobulin A - immunology</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>nanoparticles</subject><subject>Nanoparticles - administration & dosage</subject><subject>Nanoparticles - chemistry</subject><subject>Pertussis</subject><subject>pertussis toxin</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Polyelectrolytes</subject><subject>proteins</subject><subject>Sulfate</subject><subject>Toxoids</subject><subject>Toxoids - antagonists & inhibitors</subject><subject>Toxoids - immunology</subject><subject>vaccine adjuvants</subject><subject>vaccine delivery</subject><subject>Vaccines</subject><subject>Zeta potential</subject><issn>0022-3549</issn><issn>1520-6017</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90s1u1DAQB_AIgehSOPACKBJCwCGtP2I7Oa6WUrZalYotFHGxHGfS9TaJg53A7o134A15ElyyLRICTj74NzO2_46ixxgdYITI4brzB4QITu9EE8wISjjC4m40CXskoSzN96IH3q8RQhwxdj_aIzhnNM3zSbQ6aleq1VDG86YZWojfge9s6yGeXirT-j4-A9cP3hsfn9uNNWVcbOP55TRZWFWGstnK9Nar9se3769g0zvVxsuhrlQP8alqbadcb3QN_mF0r1K1h0e7dT96__rofPYmWbw9ns-mi0SnuaBJhrMiLTgpeJVTBKkGihTBFSl1kWmFC56XDCNcZoKmKRE5KTBwXlREZGmlON2Pno99O2c_D-B72Rivoa5VC3bwMkcs5ySjLMgX_5VhighH4jQN9OkfdG0H14Z7SMywoFmGUxzUy1FpZ713UMnOmUa5bWglr4OSISj5K6hgn-w6DkUD5a28SSaAZzugvFZ1FR5WG__bMYaYQFlwh6P7amrY_nuiPDlb3oxOxgrje9jcVih3JbmggsmL02O5uFieLD_iD_JT8HT0EGL7YsBJrw1c_xnjQPeytOYvF_wJo8jKlw</recordid><startdate>201201</startdate><enddate>201201</enddate><creator>Sharma, Sameer</creator><creator>Mukkur, Trilochan K.S.</creator><creator>Benson, Heather A.E.</creator><creator>Chen, Yan</creator><general>Elsevier Inc</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>American Pharmaceutical Association</general><general>Elsevier Limited</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>201201</creationdate><title>Enhanced Immune Response Against Pertussis Toxoid by IgA-Loaded Chitosan–Dextran Sulfate Nanoparticles</title><author>Sharma, Sameer ; Mukkur, Trilochan K.S. ; Benson, Heather A.E. ; Chen, Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4973-818b4b62b6f930e4ce30a21f2dcb8ca1b69d5101d873442792b1e66bf2784fa63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adjuvants</topic><topic>Adjuvants, Immunologic - administration & dosage</topic><topic>Adjuvants, Immunologic - chemistry</topic><topic>Alum Compounds - administration & dosage</topic><topic>Alum Compounds - chemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chemistry, Pharmaceutical - methods</topic><topic>chitosan</topic><topic>Chitosan - administration & dosage</topic><topic>Chitosan - chemistry</topic><topic>Chitosan - immunology</topic><topic>Coacervation</topic><topic>dextran sulfate</topic><topic>Dextran Sulfate - administration & dosage</topic><topic>Dextran Sulfate - chemistry</topic><topic>Dextran Sulfate - immunology</topic><topic>Drug Carriers - administration & dosage</topic><topic>Drug Carriers - chemistry</topic><topic>Drug Delivery Systems - methods</topic><topic>Female</topic><topic>General pharmacology</topic><topic>Immune response</topic><topic>Immunoglobulin A</topic><topic>Immunoglobulin A - administration & dosage</topic><topic>Immunoglobulin A - chemistry</topic><topic>Immunoglobulin A - immunology</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - blood</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>nanoparticles</topic><topic>Nanoparticles - administration & dosage</topic><topic>Nanoparticles - chemistry</topic><topic>Pertussis</topic><topic>pertussis toxin</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Polyelectrolytes</topic><topic>proteins</topic><topic>Sulfate</topic><topic>Toxoids</topic><topic>Toxoids - antagonists & inhibitors</topic><topic>Toxoids - immunology</topic><topic>vaccine adjuvants</topic><topic>vaccine delivery</topic><topic>Vaccines</topic><topic>Zeta potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharma, Sameer</creatorcontrib><creatorcontrib>Mukkur, Trilochan K.S.</creatorcontrib><creatorcontrib>Benson, Heather A.E.</creatorcontrib><creatorcontrib>Chen, Yan</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharma, Sameer</au><au>Mukkur, Trilochan K.S.</au><au>Benson, Heather A.E.</au><au>Chen, Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Immune Response Against Pertussis Toxoid by IgA-Loaded Chitosan–Dextran Sulfate Nanoparticles</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><addtitle>J. Pharm. Sci</addtitle><date>2012-01</date><risdate>2012</risdate><volume>101</volume><issue>1</issue><spage>233</spage><epage>244</epage><pages>233-244</pages><issn>0022-3549</issn><issn>1520-6017</issn><eissn>1520-6017</eissn><coden>JPMSAE</coden><abstract>The objective of the present study was to evaluate immunological activities of chitosan–dextran sulfate (CS–DS) nanoparticle formulation of pertussis toxoid (PTXd) and its combination with a potential immunological adjuvant, immunoglobulin A (IgA). CS–DS nanoparticles were prepared using a complex coacervation (polyelectrolyte complexation) technique. CS–DS nanoparticle formulations with size and zeta potential in a range of 300–350nm and +40–+55mV, respectively, were obtained. An entrapment efficiency of more than 90% was obtained for pertussis toxin and IgA in CS–DS nanoparticles. All loaded nanoparticle formulations showed less than 20% of release within 24h in in vitro release studies. The immunological evaluation of developed formulations in female Balb/c mice groups showed that the CS–DS nanoparticles formulations induced significantly higher serum IgG and IgG1 titers (p < 0.05) as compared with conventional alum-adjuvanted PTXd formulation administered by subcutaneous route. This study indicated the potential of CS–DS nanoparticles to be a simple and effective particulate delivery system with in-built immunological adjuvant property for acellular protein antigens. The study also revealed the potential important role of IgA-loaded CS–DS nanoparticles as a novel immunological adjuvant for vaccine delivery. © 2011 Wiley Periodicals, Inc. and the American Pharmacists Association.</abstract><cop>Hoboken</cop><pub>Elsevier Inc</pub><pmid>21953499</pmid><doi>10.1002/jps.22763</doi><tpages>12</tpages></addata></record> |
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| subjects | Adjuvants Adjuvants, Immunologic - administration & dosage Adjuvants, Immunologic - chemistry Alum Compounds - administration & dosage Alum Compounds - chemistry Animals Biological and medical sciences Chemistry, Pharmaceutical - methods chitosan Chitosan - administration & dosage Chitosan - chemistry Chitosan - immunology Coacervation dextran sulfate Dextran Sulfate - administration & dosage Dextran Sulfate - chemistry Dextran Sulfate - immunology Drug Carriers - administration & dosage Drug Carriers - chemistry Drug Delivery Systems - methods Female General pharmacology Immune response Immunoglobulin A Immunoglobulin A - administration & dosage Immunoglobulin A - chemistry Immunoglobulin A - immunology Immunoglobulin G Immunoglobulin G - blood Medical sciences Mice Mice, Inbred BALB C nanoparticles Nanoparticles - administration & dosage Nanoparticles - chemistry Pertussis pertussis toxin Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Polyelectrolytes proteins Sulfate Toxoids Toxoids - antagonists & inhibitors Toxoids - immunology vaccine adjuvants vaccine delivery Vaccines Zeta potential |
| title | Enhanced Immune Response Against Pertussis Toxoid by IgA-Loaded Chitosan–Dextran Sulfate Nanoparticles |
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