Characterization of testicular expression of P450 17α-hydroxylase, 17,20-lyase in zebrafish and its perturbation by the pharmaceutical fungicide clotrimazole
Highlights ► Cyp17-I enzyme was immunolocalized within the gonadal tissue in zebrafish. ► In vivo , clotrimazole increased cyp17a1 gene and protein expressions in testis. ► In testis explants, clotrimazole inhibited 11-ketotestosterone release. ► Clotrimazole affects differently testicular steroidog...
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| Veröffentlicht in: | General and comparative endocrinology 2011-12, Vol.174 (3), p.309-317 |
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| container_title | General and comparative endocrinology |
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| creator | Hinfray, Nathalie Baudiffier, Damien Leal, Marcelo C Porcher, Jean-Marc Aït-Aïssa, Sélim Le Gac, Florence Schulz, Rüdiger W Brion, François |
| description | Highlights ► Cyp17-I enzyme was immunolocalized within the gonadal tissue in zebrafish. ► In vivo , clotrimazole increased cyp17a1 gene and protein expressions in testis. ► In testis explants, clotrimazole inhibited 11-ketotestosterone release. ► Clotrimazole affects differently testicular steroidogenesis in vivo and ex vivo. |
| doi_str_mv | 10.1016/j.ygcen.2011.09.008 |
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| subjects | Animals Antifungal Agents - pharmacology Brain - drug effects Brain - enzymology Brain - metabolism Cells, Cultured Clotrimazole Clotrimazole - pharmacology cyp17a1 cytochrome P-450 Danio rerio endocrinology Endocrinology & Metabolism enzyme activity explants Female fish Freshwater fungicides gene expression Gene Expression Regulation, Enzymologic - drug effects genes Gonads - drug effects Gonads - enzymology Gonads - metabolism Male Pharmaceutical Primary Cell Culture protein synthesis Steroid 17-alpha-Hydroxylase - genetics Steroid 17-alpha-Hydroxylase - metabolism Steroidogenesis testes Testis - chemistry Testis - drug effects Testis - enzymology Testis - metabolism Testis culture Testosterone - analogs & derivatives Testosterone - metabolism Zebrafish Zebrafish - genetics Zebrafish - metabolism Zebrafish Proteins - genetics Zebrafish Proteins - metabolism |
| title | Characterization of testicular expression of P450 17α-hydroxylase, 17,20-lyase in zebrafish and its perturbation by the pharmaceutical fungicide clotrimazole |
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