Characterization and distribution of the gene cluster encoding RumC, an anti‐Clostridium perfringens bacteriocin produced in the gut
Ruminococcin C (RumC) is a trypsin‐dependent bacteriocin produced by Ruminococcus gnavus E1, a gram‐positive strict anaerobic strain isolated from human feces. It consists of at least three similar peptides active against Clostridium perfringens. In this article, a 15‐kb region from R. gnavus E1 chr...
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| Veröffentlicht in: | FEMS microbiology ecology 2011-11, Vol.78 (2), p.405-415 |
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| creator | Pujol, Ange Crost, Emmanuelle H. Simon, Gwenola Barbe, Valerie Vallenet, David Gomez, Ana Fons, Michel |
| description | Ruminococcin C (RumC) is a trypsin‐dependent bacteriocin produced by Ruminococcus gnavus E1, a gram‐positive strict anaerobic strain isolated from human feces. It consists of at least three similar peptides active against Clostridium perfringens. In this article, a 15‐kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumC was characterized. It harbored 17 open reading frames (called rumc genes) with predicted functions in bacteriocin biosynthesis and post‐translational modification, signal transduction regulation, and immunity. An unusual feature of the locus is the presence of five genes encoding highly homologous, but nonidentical RumC precursors. The transcription levels of the rumc genes were quantified. The rumC genes were found to be highly expressed in vivo, when R. gnavus E1 colonized the digestive tract of mono‐contaminated rats, whereas the amount of corresponding transcripts was below detection level when it grew in liquid culture medium. Moreover, the rumC‐like genes were disseminated among 10 strains (R. gnavus or related species) previously isolated from human fecal samples and selected for their capability to produce a trypsin‐dependant anti‐C. perfringens compound. All harbored at least a rumC1‐like copy, four exhibited rumC1–5 genes identical to those of strain E1. |
| doi_str_mv | 10.1111/j.1574-6941.2011.01176.x |
| format | Article |
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It consists of at least three similar peptides active against Clostridium perfringens. In this article, a 15‐kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumC was characterized. It harbored 17 open reading frames (called rumc genes) with predicted functions in bacteriocin biosynthesis and post‐translational modification, signal transduction regulation, and immunity. An unusual feature of the locus is the presence of five genes encoding highly homologous, but nonidentical RumC precursors. The transcription levels of the rumc genes were quantified. The rumC genes were found to be highly expressed in vivo, when R. gnavus E1 colonized the digestive tract of mono‐contaminated rats, whereas the amount of corresponding transcripts was below detection level when it grew in liquid culture medium. Moreover, the rumC‐like genes were disseminated among 10 strains (R. gnavus or related species) previously isolated from human fecal samples and selected for their capability to produce a trypsin‐dependant anti‐C. perfringens compound. All harbored at least a rumC1‐like copy, four exhibited rumC1–5 genes identical to those of strain E1.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2011.01176.x</identifier><identifier>PMID: 22092178</identifier><identifier>CODEN: FMECEZ</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Animal, plant and microbial ecology ; Animals ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriocins - genetics ; Bacteriocins - metabolism ; Bacteriocins - toxicity ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Biosynthesis ; Clostridium perfringens - drug effects ; Clostridium perfringens - genetics ; Clostridium perfringens - metabolism ; colonization resistance ; dissemination ; Ecology ; Feces ; Feces - microbiology ; Fundamental and applied biological sciences. Psychology ; Gastrointestinal Tract - metabolism ; Gastrointestinal Tract - microbiology ; gut microbiota ; in vivo expression ; Microbial ecology ; Microbiology ; Molecular Sequence Data ; Multigene Family ; Open Reading Frames ; Peptides ; Protein Processing, Post-Translational ; Rats ; Ruminococcus - genetics ; Ruminococcus - growth & development ; Ruminococcus - metabolism ; Ruminococcus gnavus ; Trypsin - genetics ; Trypsin - metabolism</subject><ispartof>FEMS microbiology ecology, 2011-11, Vol.78 (2), p.405-415</ispartof><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2015 INIST-CNRS</rights><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</rights><rights>Copyright © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4766-971a6c778cf1126940ac5071a40c39c20ed667a4eb22e8ad349213a7e329bd913</citedby><cites>FETCH-LOGICAL-c4766-971a6c778cf1126940ac5071a40c39c20ed667a4eb22e8ad349213a7e329bd913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6941.2011.01176.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6941.2011.01176.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24622869$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22092178$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pujol, Ange</creatorcontrib><creatorcontrib>Crost, Emmanuelle H.</creatorcontrib><creatorcontrib>Simon, Gwenola</creatorcontrib><creatorcontrib>Barbe, Valerie</creatorcontrib><creatorcontrib>Vallenet, David</creatorcontrib><creatorcontrib>Gomez, Ana</creatorcontrib><creatorcontrib>Fons, Michel</creatorcontrib><title>Characterization and distribution of the gene cluster encoding RumC, an anti‐Clostridium perfringens bacteriocin produced in the gut</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Ruminococcin C (RumC) is a trypsin‐dependent bacteriocin produced by Ruminococcus gnavus E1, a gram‐positive strict anaerobic strain isolated from human feces. It consists of at least three similar peptides active against Clostridium perfringens. In this article, a 15‐kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumC was characterized. It harbored 17 open reading frames (called rumc genes) with predicted functions in bacteriocin biosynthesis and post‐translational modification, signal transduction regulation, and immunity. An unusual feature of the locus is the presence of five genes encoding highly homologous, but nonidentical RumC precursors. The transcription levels of the rumc genes were quantified. The rumC genes were found to be highly expressed in vivo, when R. gnavus E1 colonized the digestive tract of mono‐contaminated rats, whereas the amount of corresponding transcripts was below detection level when it grew in liquid culture medium. Moreover, the rumC‐like genes were disseminated among 10 strains (R. gnavus or related species) previously isolated from human fecal samples and selected for their capability to produce a trypsin‐dependant anti‐C. perfringens compound. All harbored at least a rumC1‐like copy, four exhibited rumC1–5 genes identical to those of strain E1.</description><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriocins - genetics</subject><subject>Bacteriocins - metabolism</subject><subject>Bacteriocins - toxicity</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Clostridium perfringens - drug effects</subject><subject>Clostridium perfringens - genetics</subject><subject>Clostridium perfringens - metabolism</subject><subject>colonization resistance</subject><subject>dissemination</subject><subject>Ecology</subject><subject>Feces</subject><subject>Feces - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastrointestinal Tract - metabolism</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>gut microbiota</subject><subject>in vivo expression</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Open Reading Frames</subject><subject>Peptides</subject><subject>Protein Processing, Post-Translational</subject><subject>Rats</subject><subject>Ruminococcus - genetics</subject><subject>Ruminococcus - growth & development</subject><subject>Ruminococcus - metabolism</subject><subject>Ruminococcus gnavus</subject><subject>Trypsin - genetics</subject><subject>Trypsin - metabolism</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-K1TAUxoMoznX0FSQg4sbWJG2TZuFiKDMqjAii65AmpzO5tOk1aXDGlSvXPqNPYnrvdQRXhoQckt93_vAhhCkpaV6vtiVtRF1wWdOSEUrLfAQvb-6hzd3HfbQhlLcFryU_QY9i3BJCm6omD9EJY0QyKtoN-tFd66DNAsF904ubPdbeYuviElyf9g_zgJdrwFfgAZsxxcxi8Ga2zl_hj2nqXmZN3ov79f1nN86r1Lo04R2EIWQIfMT9ocZsnMe7MNtkwOIc7zOn5TF6MOgxwpPjfYo-X5x_6t4Wlx_evOvOLgtTC84LKajmRojWDJSyPCXRpiH5sSamkoYRsJwLXUPPGLTaVnUes9ICKiZ7K2l1il4c8uYeviSIi5pcNDCO2sOcopKk4UIIKTP57B9yO6fgc3OKNlQw2ZKKZao9UCbMMQYY1C64SYdbRYlarVJbtTqiVkfUapXaW6VusvTpsUDqJ7B3wj_eZOD5EdDR6HEI2hsX_3I1Z6zla6evD9xXN8LtfzegLs7fr1H1GwoHsOk</recordid><startdate>201111</startdate><enddate>201111</enddate><creator>Pujol, Ange</creator><creator>Crost, Emmanuelle H.</creator><creator>Simon, Gwenola</creator><creator>Barbe, Valerie</creator><creator>Vallenet, David</creator><creator>Gomez, Ana</creator><creator>Fons, Michel</creator><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201111</creationdate><title>Characterization and distribution of the gene cluster encoding RumC, an anti‐Clostridium perfringens bacteriocin produced in the gut</title><author>Pujol, Ange ; Crost, Emmanuelle H. ; Simon, Gwenola ; Barbe, Valerie ; Vallenet, David ; Gomez, Ana ; Fons, Michel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4766-971a6c778cf1126940ac5071a40c39c20ed667a4eb22e8ad349213a7e329bd913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriocins - genetics</topic><topic>Bacteriocins - metabolism</topic><topic>Bacteriocins - toxicity</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Clostridium perfringens - drug effects</topic><topic>Clostridium perfringens - genetics</topic><topic>Clostridium perfringens - metabolism</topic><topic>colonization resistance</topic><topic>dissemination</topic><topic>Ecology</topic><topic>Feces</topic><topic>Feces - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastrointestinal Tract - metabolism</topic><topic>Gastrointestinal Tract - microbiology</topic><topic>gut microbiota</topic><topic>in vivo expression</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Open Reading Frames</topic><topic>Peptides</topic><topic>Protein Processing, Post-Translational</topic><topic>Rats</topic><topic>Ruminococcus - genetics</topic><topic>Ruminococcus - growth & development</topic><topic>Ruminococcus - metabolism</topic><topic>Ruminococcus gnavus</topic><topic>Trypsin - genetics</topic><topic>Trypsin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pujol, Ange</creatorcontrib><creatorcontrib>Crost, Emmanuelle H.</creatorcontrib><creatorcontrib>Simon, Gwenola</creatorcontrib><creatorcontrib>Barbe, Valerie</creatorcontrib><creatorcontrib>Vallenet, David</creatorcontrib><creatorcontrib>Gomez, Ana</creatorcontrib><creatorcontrib>Fons, Michel</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pujol, Ange</au><au>Crost, Emmanuelle H.</au><au>Simon, Gwenola</au><au>Barbe, Valerie</au><au>Vallenet, David</au><au>Gomez, Ana</au><au>Fons, Michel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and distribution of the gene cluster encoding RumC, an anti‐Clostridium perfringens bacteriocin produced in the gut</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2011-11</date><risdate>2011</risdate><volume>78</volume><issue>2</issue><spage>405</spage><epage>415</epage><pages>405-415</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><coden>FMECEZ</coden><abstract>Ruminococcin C (RumC) is a trypsin‐dependent bacteriocin produced by Ruminococcus gnavus E1, a gram‐positive strict anaerobic strain isolated from human feces. It consists of at least three similar peptides active against Clostridium perfringens. In this article, a 15‐kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumC was characterized. It harbored 17 open reading frames (called rumc genes) with predicted functions in bacteriocin biosynthesis and post‐translational modification, signal transduction regulation, and immunity. An unusual feature of the locus is the presence of five genes encoding highly homologous, but nonidentical RumC precursors. The transcription levels of the rumc genes were quantified. The rumC genes were found to be highly expressed in vivo, when R. gnavus E1 colonized the digestive tract of mono‐contaminated rats, whereas the amount of corresponding transcripts was below detection level when it grew in liquid culture medium. Moreover, the rumC‐like genes were disseminated among 10 strains (R. gnavus or related species) previously isolated from human fecal samples and selected for their capability to produce a trypsin‐dependant anti‐C. perfringens compound. All harbored at least a rumC1‐like copy, four exhibited rumC1–5 genes identical to those of strain E1.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>22092178</pmid><doi>10.1111/j.1574-6941.2011.01176.x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animal, plant and microbial ecology Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriocins - genetics Bacteriocins - metabolism Bacteriocins - toxicity Bacteriology Base Sequence Biological and medical sciences Biosynthesis Clostridium perfringens - drug effects Clostridium perfringens - genetics Clostridium perfringens - metabolism colonization resistance dissemination Ecology Feces Feces - microbiology Fundamental and applied biological sciences. Psychology Gastrointestinal Tract - metabolism Gastrointestinal Tract - microbiology gut microbiota in vivo expression Microbial ecology Microbiology Molecular Sequence Data Multigene Family Open Reading Frames Peptides Protein Processing, Post-Translational Rats Ruminococcus - genetics Ruminococcus - growth & development Ruminococcus - metabolism Ruminococcus gnavus Trypsin - genetics Trypsin - metabolism |
| title | Characterization and distribution of the gene cluster encoding RumC, an anti‐Clostridium perfringens bacteriocin produced in the gut |
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