Synthesis of FAEEs from glycerol in engineered Saccharomyces cerevisiae using endogenously produced ethanol by heterologous expression of an unspecific bacterial acyltransferase
The high price of petroleum‐based diesel fuel has led to the development of alternative fuels, such as ethanol. Saccharomyces cerevisiae was metabolically engineered to utilize glycerol as a substrate for ethanol production. For the synthesis of fatty acid ethyl esters (FAEEs) by engineered S. cerev...
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| Veröffentlicht in: | Biotechnology and bioengineering 2012-01, Vol.109 (1), p.110-115 |
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| creator | Yu, Kyung Ok Jung, Ju Kim, Seung Wook Park, Chul Hwan Han, Sung Ok |
| description | The high price of petroleum‐based diesel fuel has led to the development of alternative fuels, such as ethanol. Saccharomyces cerevisiae was metabolically engineered to utilize glycerol as a substrate for ethanol production. For the synthesis of fatty acid ethyl esters (FAEEs) by engineered S. cerevisiae that utilize glycerol as substrate, heterologous expression of an unspecific acyltransferase from Acinetobacter baylyi with glycerol utilizing genes was established. As a result, the engineered YPH499 (pGcyaDak, pGupWs‐DgaTCas) strain produced 0.24 g/L FAEEs using endogenous ethanol produced from glycerol. And this study also demonstrated the possibility of increasing FAEE production by enhancing ethanol production by minimizing the synthesis of glycerol. The overall FAEE production in strain YPH499 fps1Δ gpd2Δ (pGcyaDak, pGupWs‐DgaTCas) was 2.1‐fold more than in YPH499 (pGcyaDak, pGupWs‐DgaTCas), with approximately 0.52 g/L FAEEs produced, while nearly 17 g/L of glycerol was consumed. These results clearly indicated that FAEEs were synthesized in engineered S. cerevisiae by esterifying exogenous fatty acids with endogenously produced ethanol from glycerol. This microbial system acts as a platform in applying metabolic engineering that allows the production of FAEEs from cheap and abundant substrates specifically glycerol through the use of endogenous bioethanol. Biotechnol. Bioeng. 2012;109: 110–115. © 2011 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bit.23311 |
| format | Article |
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Saccharomyces cerevisiae was metabolically engineered to utilize glycerol as a substrate for ethanol production. For the synthesis of fatty acid ethyl esters (FAEEs) by engineered S. cerevisiae that utilize glycerol as substrate, heterologous expression of an unspecific acyltransferase from Acinetobacter baylyi with glycerol utilizing genes was established. As a result, the engineered YPH499 (pGcyaDak, pGupWs‐DgaTCas) strain produced 0.24 g/L FAEEs using endogenous ethanol produced from glycerol. And this study also demonstrated the possibility of increasing FAEE production by enhancing ethanol production by minimizing the synthesis of glycerol. The overall FAEE production in strain YPH499 fps1Δ gpd2Δ (pGcyaDak, pGupWs‐DgaTCas) was 2.1‐fold more than in YPH499 (pGcyaDak, pGupWs‐DgaTCas), with approximately 0.52 g/L FAEEs produced, while nearly 17 g/L of glycerol was consumed. These results clearly indicated that FAEEs were synthesized in engineered S. cerevisiae by esterifying exogenous fatty acids with endogenously produced ethanol from glycerol. This microbial system acts as a platform in applying metabolic engineering that allows the production of FAEEs from cheap and abundant substrates specifically glycerol through the use of endogenous bioethanol. Biotechnol. Bioeng. 2012;109: 110–115. © 2011 Wiley Periodicals, Inc.</description><identifier>ISSN: 0006-3592</identifier><identifier>ISSN: 1097-0290</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.23311</identifier><identifier>PMID: 21858787</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Acinetobacter ; Acinetobacter - enzymology ; Acinetobacter - genetics ; acyltransferase ; Acyltransferases - genetics ; Acyltransferases - metabolism ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bioreactors ; Chemical synthesis ; endogenously produced ethanol ; Ethanol ; Ethanol - metabolism ; fatty acid ethyl esters ; Fatty acids ; Fatty Acids - metabolism ; Fermentation ; glycerol ; Glycerol - metabolism ; Gram-negative bacteria ; Metabolic Engineering ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Yeast</subject><ispartof>Biotechnology and bioengineering, 2012-01, Vol.109 (1), p.110-115</ispartof><rights>Copyright © 2011 Wiley Periodicals, Inc.</rights><rights>Copyright John Wiley and Sons, Limited Jan 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4881-54eff49210c647c41c74e84998ad19bfc7bc256b91fe1fd8fe02eebb0b0f17553</citedby><cites>FETCH-LOGICAL-c4881-54eff49210c647c41c74e84998ad19bfc7bc256b91fe1fd8fe02eebb0b0f17553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.23311$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.23311$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21858787$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Kyung Ok</creatorcontrib><creatorcontrib>Jung, Ju</creatorcontrib><creatorcontrib>Kim, Seung Wook</creatorcontrib><creatorcontrib>Park, Chul Hwan</creatorcontrib><creatorcontrib>Han, Sung Ok</creatorcontrib><title>Synthesis of FAEEs from glycerol in engineered Saccharomyces cerevisiae using endogenously produced ethanol by heterologous expression of an unspecific bacterial acyltransferase</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>The high price of petroleum‐based diesel fuel has led to the development of alternative fuels, such as ethanol. Saccharomyces cerevisiae was metabolically engineered to utilize glycerol as a substrate for ethanol production. For the synthesis of fatty acid ethyl esters (FAEEs) by engineered S. cerevisiae that utilize glycerol as substrate, heterologous expression of an unspecific acyltransferase from Acinetobacter baylyi with glycerol utilizing genes was established. As a result, the engineered YPH499 (pGcyaDak, pGupWs‐DgaTCas) strain produced 0.24 g/L FAEEs using endogenous ethanol produced from glycerol. And this study also demonstrated the possibility of increasing FAEE production by enhancing ethanol production by minimizing the synthesis of glycerol. The overall FAEE production in strain YPH499 fps1Δ gpd2Δ (pGcyaDak, pGupWs‐DgaTCas) was 2.1‐fold more than in YPH499 (pGcyaDak, pGupWs‐DgaTCas), with approximately 0.52 g/L FAEEs produced, while nearly 17 g/L of glycerol was consumed. These results clearly indicated that FAEEs were synthesized in engineered S. cerevisiae by esterifying exogenous fatty acids with endogenously produced ethanol from glycerol. This microbial system acts as a platform in applying metabolic engineering that allows the production of FAEEs from cheap and abundant substrates specifically glycerol through the use of endogenous bioethanol. Biotechnol. Bioeng. 2012;109: 110–115. © 2011 Wiley Periodicals, Inc.</description><subject>Acinetobacter</subject><subject>Acinetobacter - enzymology</subject><subject>Acinetobacter - genetics</subject><subject>acyltransferase</subject><subject>Acyltransferases - genetics</subject><subject>Acyltransferases - metabolism</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bioreactors</subject><subject>Chemical synthesis</subject><subject>endogenously produced ethanol</subject><subject>Ethanol</subject><subject>Ethanol - metabolism</subject><subject>fatty acid ethyl esters</subject><subject>Fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Fermentation</subject><subject>glycerol</subject><subject>Glycerol - metabolism</subject><subject>Gram-negative bacteria</subject><subject>Metabolic Engineering</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Yeast</subject><issn>0006-3592</issn><issn>1097-0290</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90U1vFCEYB3BiNHatHvwChnhRD9PCvAHHbt2-mI3GbI1HAszDLHWW2cKMdj6W37Cs2_ZgoidC-D1_IH-EXlNyRAnJj7UbjvKioPQJmlEiWEZyQZ6iGSGkzopK5AfoRYzXact4XT9HBznlFWeczdDv1eSHNUQXcW_x2cliEbEN_Qa33WQg9B12HoNvnQcI0OCVMmatEkinEScBP110CvAYnW-TbPoWfD_GbsLb0DejSUMwrJVPUXrCaxh2qX2bCIbbbYAYXe93lyuPRx-3YJx1BmtlknSqw8pM3RCUjxaCivASPbOqi_Dqfj1E384WV6cX2fLL-eXpyTIzJec0q0qwthQ5JaYumSmpYSXwUgiuGiq0NUybvKq1oBaobbgFkgNoTTSxlFVVcYje7XPTN25GiIPcuGig65SH9HgpSFWzStA8yff_lZRQJmrCRZno27_odT8Gn_6xy2O0ELxO6MMemdDHGMDKbXAbFaaUJHeFy1S4_FN4sm_uA0e9geZRPjScwPEe_HIdTP9OkvPLq4fIbD_h4gC3jxMq_JA1K1glv38-l6s5_0iW80_ya3EHS4vIiw</recordid><startdate>201201</startdate><enddate>201201</enddate><creator>Yu, Kyung Ok</creator><creator>Jung, Ju</creator><creator>Kim, Seung Wook</creator><creator>Park, Chul Hwan</creator><creator>Han, Sung Ok</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7QL</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>201201</creationdate><title>Synthesis of FAEEs from glycerol in engineered Saccharomyces cerevisiae using endogenously produced ethanol by heterologous expression of an unspecific bacterial acyltransferase</title><author>Yu, Kyung Ok ; 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Bioeng</addtitle><date>2012-01</date><risdate>2012</risdate><volume>109</volume><issue>1</issue><spage>110</spage><epage>115</epage><pages>110-115</pages><issn>0006-3592</issn><issn>1097-0290</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>The high price of petroleum‐based diesel fuel has led to the development of alternative fuels, such as ethanol. Saccharomyces cerevisiae was metabolically engineered to utilize glycerol as a substrate for ethanol production. For the synthesis of fatty acid ethyl esters (FAEEs) by engineered S. cerevisiae that utilize glycerol as substrate, heterologous expression of an unspecific acyltransferase from Acinetobacter baylyi with glycerol utilizing genes was established. As a result, the engineered YPH499 (pGcyaDak, pGupWs‐DgaTCas) strain produced 0.24 g/L FAEEs using endogenous ethanol produced from glycerol. And this study also demonstrated the possibility of increasing FAEE production by enhancing ethanol production by minimizing the synthesis of glycerol. The overall FAEE production in strain YPH499 fps1Δ gpd2Δ (pGcyaDak, pGupWs‐DgaTCas) was 2.1‐fold more than in YPH499 (pGcyaDak, pGupWs‐DgaTCas), with approximately 0.52 g/L FAEEs produced, while nearly 17 g/L of glycerol was consumed. These results clearly indicated that FAEEs were synthesized in engineered S. cerevisiae by esterifying exogenous fatty acids with endogenously produced ethanol from glycerol. This microbial system acts as a platform in applying metabolic engineering that allows the production of FAEEs from cheap and abundant substrates specifically glycerol through the use of endogenous bioethanol. Biotechnol. Bioeng. 2012;109: 110–115. © 2011 Wiley Periodicals, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>21858787</pmid><doi>10.1002/bit.23311</doi><tpages>6</tpages></addata></record> |
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| subjects | Acinetobacter Acinetobacter - enzymology Acinetobacter - genetics acyltransferase Acyltransferases - genetics Acyltransferases - metabolism Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Bioreactors Chemical synthesis endogenously produced ethanol Ethanol Ethanol - metabolism fatty acid ethyl esters Fatty acids Fatty Acids - metabolism Fermentation glycerol Glycerol - metabolism Gram-negative bacteria Metabolic Engineering Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Yeast |
| title | Synthesis of FAEEs from glycerol in engineered Saccharomyces cerevisiae using endogenously produced ethanol by heterologous expression of an unspecific bacterial acyltransferase |
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