Molecular cloning, bioinformatics analysis and functional characterization of B-cell activating factor in goat ( Capra hircus)

► In this study, we first cloned the B-cell activating factor (BAFF) gene in goat ( Capra hircus). ► The sequence alignment and 3D structure were analyzed using bioinformatics methods. ► Real-time quantification PCR tested the tissue distribution. ► Protein was expressed in Escherichia coli and puri...

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Veröffentlicht in:	Developmental and comparative immunology 2012, Vol.36 (1), p.191-198
Hauptverfasser:	Shen, Yuefen, You, Fengtao, Li, Chunlan, He, Zhijuan, Liang, Zhenning, Ai, Hongxin, Wang, Shule, Zhang, Shuangquan
Format:	Artikel
Sprache:	eng
Schlagworte:	3D structure affinity chromatography Animals B-Cell Activating Factor - genetics B-Cell Activating Factor - immunology B-Cell Activating Factor - metabolism B-cell survival/proliferation B-lymphocytes B-Lymphocytes - immunology B-Lymphocytes - metabolism B-Lymphocytes - pathology Base Sequence bioinformatics Capra hircus Cell Proliferation Cell Separation cell viability Cloning, Molecular complementary DNA Computational Biology cysteine Escherichia coli Flow cytometric analysis Flow Cytometry Gene Expression Profiling genes Goat BAFF Goats immune response immunoglobulins Lymphocyte Activation Male messenger RNA molecular cloning Molecular Sequence Data open reading frames polyacrylamide gel electrophoresis Protein Conformation proteinases quantitative polymerase chain reaction Real-time quantitative PCR reverse transcriptase polymerase chain reaction secretion Sequence Alignment sequence analysis spleen SUMO-gsBAFF Transgenes - genetics tumor necrosis factors Western blotting
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