Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis
Comparative genomics of Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present signifi...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2011-09, Vol.143 (1), p.75-82 |
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| creator | Souza, Giliane S. Rodrigues, Ana Bárbara F. Gioffré, Andrea Romano, Maria I. Carvalho, Eulógio C.Q. Ventura, Thatiana L.B. Lasunskaia, Elena B. |
| description | Comparative genomics of
Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present significant variability in primary sequence length and composition between members of
M. avium and
M. tuberculosis complexes. In this study, the recombinant Apa protein encoded by the MAP1569/
ModD gene of
M. avium subsp.
paratuberculosis (Map) was used to generate a panel of monoclonal antibodies which were shown to recognize the most important veterinary pathogens of the
M. avium complex, specifically Map and
M. avium subsp.
hominissuis, and which did not cross-react with
M. bovis or
M. tuberculosis. The produced antibodies were demonstrated to be a useful tool for the species-specific immunofluorescence or immunohistochemical detection of Map in experimentally infected cell cultures or intestinal tissues from cattle with bovine paratuberculosis and, additionally, they may be employed for the discrimination of pathogenic
M. avium subspecies via Western blotting. |
| doi_str_mv | 10.1016/j.vetimm.2011.06.026 |
| format | Article |
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Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present significant variability in primary sequence length and composition between members of
M. avium and
M. tuberculosis complexes. In this study, the recombinant Apa protein encoded by the MAP1569/
ModD gene of
M. avium subsp.
paratuberculosis (Map) was used to generate a panel of monoclonal antibodies which were shown to recognize the most important veterinary pathogens of the
M. avium complex, specifically Map and
M. avium subsp.
hominissuis, and which did not cross-react with
M. bovis or
M. tuberculosis. The produced antibodies were demonstrated to be a useful tool for the species-specific immunofluorescence or immunohistochemical detection of Map in experimentally infected cell cultures or intestinal tissues from cattle with bovine paratuberculosis and, additionally, they may be employed for the discrimination of pathogenic
M. avium subspecies via Western blotting.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2011.06.026</identifier><identifier>PMID: 21752474</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies, Bacterial ; Antibodies, Monoclonal ; Antigen Apa ; Antigens, Bacterial - genetics ; Bacterial Proteins - genetics ; Bacterial Proteins - immunology ; Cattle ; Cattle Diseases - diagnosis ; Cattle Diseases - microbiology ; Fluorescent Antibody Technique ; Genes, Bacterial ; Immunodetection ; M. avium subsp. paratuberculosis ; Macrophages - microbiology ; Mice ; Monoclonal antibodies ; Mycobacterium avium ; Mycobacterium avium subsp. paratuberculosis - genetics ; Mycobacterium avium subsp. paratuberculosis - immunology ; Paratuberculosis ; Paratuberculosis - diagnosis ; Paratuberculosis - microbiology ; Species Specificity</subject><ispartof>Veterinary immunology and immunopathology, 2011-09, Vol.143 (1), p.75-82</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-569a51fea12cb63d51e4edf23b3d7bf183988de6377548ec7c3ed5b3f87f60aa3</citedby><cites>FETCH-LOGICAL-c439t-569a51fea12cb63d51e4edf23b3d7bf183988de6377548ec7c3ed5b3f87f60aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetimm.2011.06.026$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21752474$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Souza, Giliane S.</creatorcontrib><creatorcontrib>Rodrigues, Ana Bárbara F.</creatorcontrib><creatorcontrib>Gioffré, Andrea</creatorcontrib><creatorcontrib>Romano, Maria I.</creatorcontrib><creatorcontrib>Carvalho, Eulógio C.Q.</creatorcontrib><creatorcontrib>Ventura, Thatiana L.B.</creatorcontrib><creatorcontrib>Lasunskaia, Elena B.</creatorcontrib><title>Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>Comparative genomics of
Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present significant variability in primary sequence length and composition between members of
M. avium and
M. tuberculosis complexes. In this study, the recombinant Apa protein encoded by the MAP1569/
ModD gene of
M. avium subsp.
paratuberculosis (Map) was used to generate a panel of monoclonal antibodies which were shown to recognize the most important veterinary pathogens of the
M. avium complex, specifically Map and
M. avium subsp.
hominissuis, and which did not cross-react with
M. bovis or
M. tuberculosis. The produced antibodies were demonstrated to be a useful tool for the species-specific immunofluorescence or immunohistochemical detection of Map in experimentally infected cell cultures or intestinal tissues from cattle with bovine paratuberculosis and, additionally, they may be employed for the discrimination of pathogenic
M. avium subspecies via Western blotting.</description><subject>Animals</subject><subject>Antibodies, Bacterial</subject><subject>Antibodies, Monoclonal</subject><subject>Antigen Apa</subject><subject>Antigens, Bacterial - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - immunology</subject><subject>Cattle</subject><subject>Cattle Diseases - diagnosis</subject><subject>Cattle Diseases - microbiology</subject><subject>Fluorescent Antibody Technique</subject><subject>Genes, Bacterial</subject><subject>Immunodetection</subject><subject>M. avium subsp. paratuberculosis</subject><subject>Macrophages - microbiology</subject><subject>Mice</subject><subject>Monoclonal antibodies</subject><subject>Mycobacterium avium</subject><subject>Mycobacterium avium subsp. paratuberculosis - genetics</subject><subject>Mycobacterium avium subsp. paratuberculosis - immunology</subject><subject>Paratuberculosis</subject><subject>Paratuberculosis - diagnosis</subject><subject>Paratuberculosis - microbiology</subject><subject>Species Specificity</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2KFTEQhRtRnOvoG4hk56rb_Kd7IwyDfzDiRtchnVRmcunutEn6yjyVr2ju3KsbQaFILfJVneKcpnlJcEcwkW_23QFKmOeOYkI6LDtM5aNmR3rFWioYf9zsKiZayqm6aJ7lvMcYi6HvnzYXlChBueK75ufVapBZSriFBUWPPt_bOBpbIIVtRuZwfPM25rVDq0mmbCMku00xh4xMLVRMuoWCfEwor2AD5Pah-2BRvW5booMCtoT4sL_cATrvNygstXz9BIdKyHmDfGSsKWUC9COUu79EnzdPvJkyvDj3y-bb-3dfrz-2N18-fLq-umktZ0NphRyMIB4MoXaUzAkCHJynbGROjZ70rBrhQDKlBO_BKsvAiZH5XnmJjWGXzevT3jXF7_WuoueQLUyTWSBuWQ-Y80EOiv-X7Ac6cCGVqCQ_kTbFnBN4vaYwm3SvCdbHTPVenzLVx0w1lrpmWsdenQW2cQb3Z-h3iBV4ewKgGnIIkHSuOSwWXEjVXO1i-LfCL8B-uhQ</recordid><startdate>20110915</startdate><enddate>20110915</enddate><creator>Souza, Giliane S.</creator><creator>Rodrigues, Ana Bárbara F.</creator><creator>Gioffré, Andrea</creator><creator>Romano, Maria I.</creator><creator>Carvalho, Eulógio C.Q.</creator><creator>Ventura, Thatiana L.B.</creator><creator>Lasunskaia, Elena B.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>20110915</creationdate><title>Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis</title><author>Souza, Giliane S. ; Rodrigues, Ana Bárbara F. ; Gioffré, Andrea ; Romano, Maria I. ; Carvalho, Eulógio C.Q. ; Ventura, Thatiana L.B. ; Lasunskaia, Elena B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-569a51fea12cb63d51e4edf23b3d7bf183988de6377548ec7c3ed5b3f87f60aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial</topic><topic>Antibodies, Monoclonal</topic><topic>Antigen Apa</topic><topic>Antigens, Bacterial - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - immunology</topic><topic>Cattle</topic><topic>Cattle Diseases - diagnosis</topic><topic>Cattle Diseases - microbiology</topic><topic>Fluorescent Antibody Technique</topic><topic>Genes, Bacterial</topic><topic>Immunodetection</topic><topic>M. avium subsp. paratuberculosis</topic><topic>Macrophages - microbiology</topic><topic>Mice</topic><topic>Monoclonal antibodies</topic><topic>Mycobacterium avium</topic><topic>Mycobacterium avium subsp. paratuberculosis - genetics</topic><topic>Mycobacterium avium subsp. paratuberculosis - immunology</topic><topic>Paratuberculosis</topic><topic>Paratuberculosis - diagnosis</topic><topic>Paratuberculosis - microbiology</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Souza, Giliane S.</creatorcontrib><creatorcontrib>Rodrigues, Ana Bárbara F.</creatorcontrib><creatorcontrib>Gioffré, Andrea</creatorcontrib><creatorcontrib>Romano, Maria I.</creatorcontrib><creatorcontrib>Carvalho, Eulógio C.Q.</creatorcontrib><creatorcontrib>Ventura, Thatiana L.B.</creatorcontrib><creatorcontrib>Lasunskaia, Elena B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Souza, Giliane S.</au><au>Rodrigues, Ana Bárbara F.</au><au>Gioffré, Andrea</au><au>Romano, Maria I.</au><au>Carvalho, Eulógio C.Q.</au><au>Ventura, Thatiana L.B.</au><au>Lasunskaia, Elena B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2011-09-15</date><risdate>2011</risdate><volume>143</volume><issue>1</issue><spage>75</spage><epage>82</epage><pages>75-82</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>Comparative genomics of
Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present significant variability in primary sequence length and composition between members of
M. avium and
M. tuberculosis complexes. In this study, the recombinant Apa protein encoded by the MAP1569/
ModD gene of
M. avium subsp.
paratuberculosis (Map) was used to generate a panel of monoclonal antibodies which were shown to recognize the most important veterinary pathogens of the
M. avium complex, specifically Map and
M. avium subsp.
hominissuis, and which did not cross-react with
M. bovis or
M. tuberculosis. The produced antibodies were demonstrated to be a useful tool for the species-specific immunofluorescence or immunohistochemical detection of Map in experimentally infected cell cultures or intestinal tissues from cattle with bovine paratuberculosis and, additionally, they may be employed for the discrimination of pathogenic
M. avium subspecies via Western blotting.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21752474</pmid><doi>10.1016/j.vetimm.2011.06.026</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary immunology and immunopathology, 2011-09, Vol.143 (1), p.75-82 |
| issn | 0165-2427 1873-2534 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_904496974 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Bacterial Antibodies, Monoclonal Antigen Apa Antigens, Bacterial - genetics Bacterial Proteins - genetics Bacterial Proteins - immunology Cattle Cattle Diseases - diagnosis Cattle Diseases - microbiology Fluorescent Antibody Technique Genes, Bacterial Immunodetection M. avium subsp. paratuberculosis Macrophages - microbiology Mice Monoclonal antibodies Mycobacterium avium Mycobacterium avium subsp. paratuberculosis - genetics Mycobacterium avium subsp. paratuberculosis - immunology Paratuberculosis Paratuberculosis - diagnosis Paratuberculosis - microbiology Species Specificity |
| title | Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis |
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