Dihydroartemisinin shift the immune response towards Th1, inhibit the tumor growth in vitro and in vivo

► DHA reduces tumor cell growth in RIN cell line, and also inhibits the growth of tumor tissue in vivo. ► Administration of DHA to tumor-bearing mice increases the level of IFN-γ and decreases the level of IL-4. ► Administration of DHA to tumor-bearing mice decreases the level of splenic CD4+CD25+ F...

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Veröffentlicht in:Cellular immunology 2011, Vol.271 (1), p.67-72
Hauptverfasser: Noori, Shokoofe, Hassan, Zuhair M.
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description ► DHA reduces tumor cell growth in RIN cell line, and also inhibits the growth of tumor tissue in vivo. ► Administration of DHA to tumor-bearing mice increases the level of IFN-γ and decreases the level of IL-4. ► Administration of DHA to tumor-bearing mice decreases the level of splenic CD4+CD25+ FOXP3+ regulatory T cells. ► By using an appropriate dose of DHA, new therapeutic aspects of this medicine in cancer therapy will be elucidated. Some investigators have been found that Artemisinin and its derivates have inhibitory effect on growth of cancer cells. Among these derivatives, Dihydroartemisinin (DHA) is well known as a semi-synthetic one. In addition, T cells are proved to be essential for the destruction of cancer cells. In this research, we assessed the effects of DHA on tumor cell growth inhibition in vitro by MTT assay and in vivo by intra tumor injection of DHA against breast cancer. The results showed that the IC50 values of DHA for RIN pancreatic tumor cell line were 30μM and significant decrease in the tumor size in vivo. Also we evaluate the effect of DHA on the modulation of immune response in tumor bearing animals; these include the splenocyte proliferation using the BrdU kit; measurement of cytokine profile by ELISA, and evaluate the percentage of T regulatory cells in the spleen by flowcytometry. Our results demonstrated that a significant decrease in the level of IL-4 in the animals treated with DHA and significant decreased in the level of splenic CD4+CD25+ Foxp3+ T regulatory cells.
doi_str_mv 10.1016/j.cellimm.2011.06.008
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subjects Animals
Antimalarials - administration & dosage
Antimalarials - pharmacology
Artemisinins - administration & dosage
Artemisinins - pharmacology
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
Cell Survival - immunology
Dihydroartemisinin
Dose-Response Relationship, Drug
Female
Hindlimb - drug effects
Hindlimb - immunology
Hypersensitivity, Delayed - immunology
IFN-γ
IL-4
Immuno-modulator
Inhibitory Concentration 50
Injections, Intraperitoneal
Interferon-gamma - immunology
Interferon-gamma - metabolism
Interleukin-4 - immunology
Interleukin-4 - metabolism
Mammary Neoplasms, Experimental - immunology
Mammary Neoplasms, Experimental - pathology
Mammary Neoplasms, Experimental - prevention & control
Mice
Mice, Inbred BALB C
Sheep
T regulatory cell (Treg cell)
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
T-Lymphocytes, Regulatory - drug effects
T-Lymphocytes, Regulatory - immunology
T-Lymphocytes, Regulatory - metabolism
Th1 Cells - drug effects
Th1 Cells - immunology
Th1 Cells - metabolism
Tumor Burden - drug effects
Tumor Burden - immunology
title Dihydroartemisinin shift the immune response towards Th1, inhibit the tumor growth in vitro and in vivo
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