A new method for producing biologically active nanocomplexes by a noncovalent conjugation of proteins with viral particles
A new method for noncovalent immobilization of a peptide epitope on the virion surface was developed to simplify and standardize the procedures for producing viral nanocomplexes. The efficacy of this approach was demonstrated by the example of a model system comprising the tobacco mosaic virus, synt...
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| Veröffentlicht in: | Russian journal of bioorganic chemistry 2011-07, Vol.37 (4), p.441-447 |
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| container_end_page | 447 |
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| container_issue | 4 |
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| container_title | Russian journal of bioorganic chemistry |
| container_volume | 37 |
| creator | Yaroslavov, A. A. Kaplan, I. B. Erokhina, T. N. Morozov, S. Yu Solovyev, A. G. Leshchiner, A. D. Rakhnyanskaya, A. A. Malinin, A. S. Stepanova, L. A. Kiselev, O. I. Atabekov, J. G. |
| description | A new method for noncovalent immobilization of a peptide epitope on the virion surface was developed to simplify and standardize the procedures for producing viral nanocomplexes. The efficacy of this approach was demonstrated by the example of a model system comprising the tobacco mosaic virus, synthetic cationic polymer poly-
N
-ethyl-4-vinylpyridinium bromide, and a model polypeptide. The principle of sequential adsorption, underlying production of the triple system virion-polycation-protein, was used for electrostatic immobilization of a recombinant hydrophilic fragment of the influenza virus hemagglutinin (Flu1-3) on the virion surface. The method provided for a significant increase in the immunogenic activity of this potential artificial vaccine protein. |
| doi_str_mv | 10.1134/S1068162011040169 |
| format | Article |
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N
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N
-ethyl-4-vinylpyridinium bromide, and a model polypeptide. The principle of sequential adsorption, underlying production of the triple system virion-polycation-protein, was used for electrostatic immobilization of a recombinant hydrophilic fragment of the influenza virus hemagglutinin (Flu1-3) on the virion surface. The method provided for a significant increase in the immunogenic activity of this potential artificial vaccine protein.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Influenza virus</subject><subject>Life Sciences</subject><subject>Organic Chemistry</subject><subject>Tobacco mosaic virus</subject><issn>1068-1620</issn><issn>1608-330X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9UDtPwzAQjhBIlMIPYPPGFLDjxLXHquIlVWKgA1vkuOfUlWMX22kpv55EZUNiON3pvofuviy7JfieEFo-vBPMOGEFJgSXmDBxlk0IwzynFH-cD_MA5yN-mV3FuMWYYFzxSfY9Rw4OqIO08WukfUC74Ne9Mq5FjfHWt0ZJa49IqmT2gJx0XvluZ-ELImqGPXLeKb-XFlxCyrtt38pkvENej14JjIvoYNIG7U2QFu1kSEZZiNfZhZY2ws1vn2arp8fV4iVfvj2_LubLXNECp7wAKRqhOS0KphreaMrXutSglaCCUc1nvOGMlCVRY0nGMDSVEFqrGS8bOs3uTrbDMZ89xFR3JiqwVjrwfawFLktRUVINTHJiquBjDKDrXTCdDMea4HpMuf6T8qApTpo4cF0Lod76Prjhn39EP0YZgaA</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Yaroslavov, A. A.</creator><creator>Kaplan, I. B.</creator><creator>Erokhina, T. N.</creator><creator>Morozov, S. Yu</creator><creator>Solovyev, A. G.</creator><creator>Leshchiner, A. D.</creator><creator>Rakhnyanskaya, A. A.</creator><creator>Malinin, A. S.</creator><creator>Stepanova, L. A.</creator><creator>Kiselev, O. I.</creator><creator>Atabekov, J. G.</creator><general>SP MAIK Nauka/Interperiodica</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20110701</creationdate><title>A new method for producing biologically active nanocomplexes by a noncovalent conjugation of proteins with viral particles</title><author>Yaroslavov, A. A. ; Kaplan, I. B. ; Erokhina, T. N. ; Morozov, S. Yu ; Solovyev, A. G. ; Leshchiner, A. D. ; Rakhnyanskaya, A. A. ; Malinin, A. S. ; Stepanova, L. A. ; Kiselev, O. I. ; Atabekov, J. G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-2ea9b9f83226cb8bf38df4fefc93963f878b861441c441ca660eb599ffc784b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Influenza virus</topic><topic>Life Sciences</topic><topic>Organic Chemistry</topic><topic>Tobacco mosaic virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yaroslavov, A. A.</creatorcontrib><creatorcontrib>Kaplan, I. B.</creatorcontrib><creatorcontrib>Erokhina, T. N.</creatorcontrib><creatorcontrib>Morozov, S. Yu</creatorcontrib><creatorcontrib>Solovyev, A. G.</creatorcontrib><creatorcontrib>Leshchiner, A. D.</creatorcontrib><creatorcontrib>Rakhnyanskaya, A. A.</creatorcontrib><creatorcontrib>Malinin, A. S.</creatorcontrib><creatorcontrib>Stepanova, L. A.</creatorcontrib><creatorcontrib>Kiselev, O. I.</creatorcontrib><creatorcontrib>Atabekov, J. G.</creatorcontrib><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Russian journal of bioorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yaroslavov, A. A.</au><au>Kaplan, I. B.</au><au>Erokhina, T. N.</au><au>Morozov, S. Yu</au><au>Solovyev, A. G.</au><au>Leshchiner, A. D.</au><au>Rakhnyanskaya, A. A.</au><au>Malinin, A. S.</au><au>Stepanova, L. A.</au><au>Kiselev, O. I.</au><au>Atabekov, J. G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new method for producing biologically active nanocomplexes by a noncovalent conjugation of proteins with viral particles</atitle><jtitle>Russian journal of bioorganic chemistry</jtitle><stitle>Russ J Bioorg Chem</stitle><date>2011-07-01</date><risdate>2011</risdate><volume>37</volume><issue>4</issue><spage>441</spage><epage>447</epage><pages>441-447</pages><issn>1068-1620</issn><eissn>1608-330X</eissn><abstract>A new method for noncovalent immobilization of a peptide epitope on the virion surface was developed to simplify and standardize the procedures for producing viral nanocomplexes. The efficacy of this approach was demonstrated by the example of a model system comprising the tobacco mosaic virus, synthetic cationic polymer poly-
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| ispartof | Russian journal of bioorganic chemistry, 2011-07, Vol.37 (4), p.441-447 |
| issn | 1068-1620 1608-330X |
| language | eng |
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| subjects | Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Influenza virus Life Sciences Organic Chemistry Tobacco mosaic virus |
| title | A new method for producing biologically active nanocomplexes by a noncovalent conjugation of proteins with viral particles |
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