Discrimination of Entamoeba moshkovskii in Patients with Gastrointestinal Disorders by Single-Round PCR
Entamoeba moshkovskii and Entamoeba dispar are impossible to differentiate microscopically from the pathogenic species Entamoeba histolytica. There are limited data on the prevalence of these commensal parasites in Iran. We utilized a single-round PCR assay to determine the prevalence of E. moshkovs...
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| Veröffentlicht in: | Japanese Journal of Infectious Diseases 2010/03/31, Vol.63(2), pp.136-138 |
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| creator | Mojarad, Ehsan Nazemalhosseini Nochi, Zahra Sahebekhtiari, Navid Nejad, Mohammad Rostami Dabiri, Hossein Zali, Mohammad Reza Kazemi, Bahram Haghighi, Ali |
| description | Entamoeba moshkovskii and Entamoeba dispar are impossible to differentiate microscopically from the pathogenic species Entamoeba histolytica. There are limited data on the prevalence of these commensal parasites in Iran. We utilized a single-round PCR assay to determine the prevalence of E. moshkovskii, E. dispar, and E. histolytica in stool samples from Iranian patients infected with gastrointestinal disorders. After culturing of microscopy-positive isolates and extraction of DNA, PCR was carried out to differentiate the Entamoeba isolates. Out of 3,825 stool samples examined by microscopy, 58 specimens (1.52%) were infected with E. histolytica, E. dispar, or E. moshkovskii. By PCR, 2 E. histolytica (3.45%), 53 E. dispar (91.37%), 2 E. moshkovskii (3.45%), and one mixed E. dispar/E. moshkovskii infection (1.73%) were detected. In view of the reporting of E. moshkovskii in this study in Iran and the difficulty in discriminating this ameba from two similar Entamoeba spp. by microscopy, we recommend the single-round PCR assay as an alternative tool in routine diagnosis and in epidemiological studies of amebiasis. |
| doi_str_mv | 10.7883/yoken.63.136 |
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There are limited data on the prevalence of these commensal parasites in Iran. We utilized a single-round PCR assay to determine the prevalence of E. moshkovskii, E. dispar, and E. histolytica in stool samples from Iranian patients infected with gastrointestinal disorders. After culturing of microscopy-positive isolates and extraction of DNA, PCR was carried out to differentiate the Entamoeba isolates. Out of 3,825 stool samples examined by microscopy, 58 specimens (1.52%) were infected with E. histolytica, E. dispar, or E. moshkovskii. By PCR, 2 E. histolytica (3.45%), 53 E. dispar (91.37%), 2 E. moshkovskii (3.45%), and one mixed E. dispar/E. moshkovskii infection (1.73%) were detected. In view of the reporting of E. moshkovskii in this study in Iran and the difficulty in discriminating this ameba from two similar Entamoeba spp. by microscopy, we recommend the single-round PCR assay as an alternative tool in routine diagnosis and in epidemiological studies of amebiasis.</description><identifier>ISSN: 1344-6304</identifier><identifier>EISSN: 1884-2836</identifier><identifier>DOI: 10.7883/yoken.63.136</identifier><identifier>PMID: 20332579</identifier><language>eng</language><publisher>Japan: National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</publisher><subject>Entamoeba - classification ; Entamoeba - genetics ; Entamoeba - isolation & purification ; Entamoeba dispar ; Entamoeba histolytica ; Entamoeba moshkovskii ; Entamoebiasis - diagnosis ; Entamoebiasis - parasitology ; Feces - parasitology ; Gastrointestinal Diseases - diagnosis ; Gastrointestinal Diseases - parasitology ; Humans ; Iran ; Parasitology - methods ; Polymerase Chain Reaction - methods ; Sensitivity and Specificity</subject><ispartof>Japanese Journal of Infectious Diseases, 2010/03/31, Vol.63(2), pp.136-138</ispartof><rights>2022 Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-646117a111a87f9414df01335595385d6136a41f0004063f8f016c1455793efc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20332579$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mojarad, Ehsan Nazemalhosseini</creatorcontrib><creatorcontrib>Nochi, Zahra</creatorcontrib><creatorcontrib>Sahebekhtiari, Navid</creatorcontrib><creatorcontrib>Nejad, Mohammad Rostami</creatorcontrib><creatorcontrib>Dabiri, Hossein</creatorcontrib><creatorcontrib>Zali, Mohammad Reza</creatorcontrib><creatorcontrib>Kazemi, Bahram</creatorcontrib><creatorcontrib>Haghighi, Ali</creatorcontrib><title>Discrimination of Entamoeba moshkovskii in Patients with Gastrointestinal Disorders by Single-Round PCR</title><title>Japanese Journal of Infectious Diseases</title><addtitle>Jpn J Infect Dis</addtitle><description>Entamoeba moshkovskii and Entamoeba dispar are impossible to differentiate microscopically from the pathogenic species Entamoeba histolytica. There are limited data on the prevalence of these commensal parasites in Iran. We utilized a single-round PCR assay to determine the prevalence of E. moshkovskii, E. dispar, and E. histolytica in stool samples from Iranian patients infected with gastrointestinal disorders. After culturing of microscopy-positive isolates and extraction of DNA, PCR was carried out to differentiate the Entamoeba isolates. Out of 3,825 stool samples examined by microscopy, 58 specimens (1.52%) were infected with E. histolytica, E. dispar, or E. moshkovskii. By PCR, 2 E. histolytica (3.45%), 53 E. dispar (91.37%), 2 E. moshkovskii (3.45%), and one mixed E. dispar/E. moshkovskii infection (1.73%) were detected. In view of the reporting of E. moshkovskii in this study in Iran and the difficulty in discriminating this ameba from two similar Entamoeba spp. by microscopy, we recommend the single-round PCR assay as an alternative tool in routine diagnosis and in epidemiological studies of amebiasis.</description><subject>Entamoeba - classification</subject><subject>Entamoeba - genetics</subject><subject>Entamoeba - isolation & purification</subject><subject>Entamoeba dispar</subject><subject>Entamoeba histolytica</subject><subject>Entamoeba moshkovskii</subject><subject>Entamoebiasis - diagnosis</subject><subject>Entamoebiasis - parasitology</subject><subject>Feces - parasitology</subject><subject>Gastrointestinal Diseases - diagnosis</subject><subject>Gastrointestinal Diseases - parasitology</subject><subject>Humans</subject><subject>Iran</subject><subject>Parasitology - methods</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sensitivity and Specificity</subject><issn>1344-6304</issn><issn>1884-2836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1PGzEQhi1UBIH2xrnyrZdusHdsr_fYpnxJkUDQni1n15s42bVT2wHl32MIhCMX29I8fjTvDEJnlIwrKeF861fGjQWMKYgDNKJSsqKUIL7kNzBWCCDsGJ3EuCSk5JySI3RcEoCSV_UIzf_Y2AQ7WKeT9Q77Dl-4pAdvZhoPPi5W_jGurMXW4buMGJcifrJpga90TMFbl0xM-XePs8mH1oSIZ1v8YN28N8W937gW303uv6LDTvfRfHu7T9G_y4u_k-tient1M_k1LRpO6lQIJiitNKVUy6qrGWVtRygA5zUHyVuRQ2pGO0IIIwI6mauioYznMGC6Bk7Rj513Hfz_TW5NDTmg6XvtjN9EVRPGaioE-5SsACiXQpaZ_Lkjm-BjDKZT6zwxHbaKEvWyA_W6AyVA5fYy_v1NvJkNpt3D70PPwO8dsIxJz80e0CHZpjcftvLl2Fn3xWahgzIOngGrV5pQ</recordid><startdate>20100331</startdate><enddate>20100331</enddate><creator>Mojarad, Ehsan Nazemalhosseini</creator><creator>Nochi, Zahra</creator><creator>Sahebekhtiari, Navid</creator><creator>Nejad, Mohammad Rostami</creator><creator>Dabiri, Hossein</creator><creator>Zali, Mohammad Reza</creator><creator>Kazemi, Bahram</creator><creator>Haghighi, Ali</creator><general>National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope></search><sort><creationdate>20100331</creationdate><title>Discrimination of Entamoeba moshkovskii in Patients with Gastrointestinal Disorders by Single-Round PCR</title><author>Mojarad, Ehsan Nazemalhosseini ; 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There are limited data on the prevalence of these commensal parasites in Iran. We utilized a single-round PCR assay to determine the prevalence of E. moshkovskii, E. dispar, and E. histolytica in stool samples from Iranian patients infected with gastrointestinal disorders. After culturing of microscopy-positive isolates and extraction of DNA, PCR was carried out to differentiate the Entamoeba isolates. Out of 3,825 stool samples examined by microscopy, 58 specimens (1.52%) were infected with E. histolytica, E. dispar, or E. moshkovskii. By PCR, 2 E. histolytica (3.45%), 53 E. dispar (91.37%), 2 E. moshkovskii (3.45%), and one mixed E. dispar/E. moshkovskii infection (1.73%) were detected. 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| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; EZB-FREE-00999 freely available EZB journals |
| subjects | Entamoeba - classification Entamoeba - genetics Entamoeba - isolation & purification Entamoeba dispar Entamoeba histolytica Entamoeba moshkovskii Entamoebiasis - diagnosis Entamoebiasis - parasitology Feces - parasitology Gastrointestinal Diseases - diagnosis Gastrointestinal Diseases - parasitology Humans Iran Parasitology - methods Polymerase Chain Reaction - methods Sensitivity and Specificity |
| title | Discrimination of Entamoeba moshkovskii in Patients with Gastrointestinal Disorders by Single-Round PCR |
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