Sonication-assisted Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill] embryogenic suspension culture tissue
Successful transformation of plant tissue using Agrobacterium relies on several factors including bacterial infection, host recognition, and transformation competency of the target tissue. Although soybean [Glycine max (L.) Merrill] embryogenic suspension cultures have been transformed via particle...
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| Veröffentlicht in: | Plant cell reports 1998-04, Vol.17 (6/7), p.482-488 |
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| description | Successful transformation of plant tissue using Agrobacterium relies on several factors including bacterial infection, host recognition, and transformation competency of the target tissue. Although soybean [Glycine max (L.) Merrill] embryogenic suspension cultures have been transformed via particle bombardment, Agrobacterium-mediated transformation of this tissue has not been demonstrated. We report here transformation of embryogenic suspension cultures of soybean using "Sonication-Assisted Agrobacterium-mediated Transformation" (SAAT). For SAAT of suspension culture tissue, 10-20 embryogenic clumps (2-4 mm in diameter) were inoculated with 1 ml of diluted (OD600nm 0.1-0.5) log phase Agrobacterium and sonicated for 0-300 s. After 2 days of co-culture in a maintenance medium containing 100 micromolar acetosyringone, the medium was removed and replaced with fresh maintenance medium containing 400 mg/1 Timentin. Two weeks after SAAT, the tissue was placed in maintenance medium containing 20 mg/1 hygromycin and 400 mg/1 Timentin, and the medium was replenished every week thereafter. Transgenic clones were observed and isolated 6-8 weeks following SAAT. When SAAT was not used, hygromycin-resistant clones were not obtained. Southern hybridization analyses of transformed embryogenic tissue confirmed T-DNA integration. |
| doi_str_mv | 10.1007/s002990050429 |
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After 2 days of co-culture in a maintenance medium containing 100 micromolar acetosyringone, the medium was removed and replaced with fresh maintenance medium containing 400 mg/1 Timentin. Two weeks after SAAT, the tissue was placed in maintenance medium containing 20 mg/1 hygromycin and 400 mg/1 Timentin, and the medium was replenished every week thereafter. Transgenic clones were observed and isolated 6-8 weeks following SAAT. When SAAT was not used, hygromycin-resistant clones were not obtained. Southern hybridization analyses of transformed embryogenic tissue confirmed T-DNA integration.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s002990050429</identifier><identifier>PMID: 30736623</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Agrobacterium ; Agrobacterium tumefaciens ; Bacterial diseases ; Biological and medical sciences ; Biotechnology ; cell suspension culture ; Cloning ; developmental stages ; Fundamental and applied biological sciences. Psychology ; gene expression ; gene transfer ; Genetic engineering ; Genetic technics ; genetic transformation ; Glycine max ; methodology ; Methods. Procedures. Technologies ; Miscellaneous ; plant anatomy ; Plant tissues ; Soybeans ; Synthetic digonucleotides and genes. 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Merrill] embryogenic suspension culture tissue</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>Successful transformation of plant tissue using Agrobacterium relies on several factors including bacterial infection, host recognition, and transformation competency of the target tissue. Although soybean [Glycine max (L.) Merrill] embryogenic suspension cultures have been transformed via particle bombardment, Agrobacterium-mediated transformation of this tissue has not been demonstrated. We report here transformation of embryogenic suspension cultures of soybean using "Sonication-Assisted Agrobacterium-mediated Transformation" (SAAT). For SAAT of suspension culture tissue, 10-20 embryogenic clumps (2-4 mm in diameter) were inoculated with 1 ml of diluted (OD600nm 0.1-0.5) log phase Agrobacterium and sonicated for 0-300 s. After 2 days of co-culture in a maintenance medium containing 100 micromolar acetosyringone, the medium was removed and replaced with fresh maintenance medium containing 400 mg/1 Timentin. Two weeks after SAAT, the tissue was placed in maintenance medium containing 20 mg/1 hygromycin and 400 mg/1 Timentin, and the medium was replenished every week thereafter. Transgenic clones were observed and isolated 6-8 weeks following SAAT. When SAAT was not used, hygromycin-resistant clones were not obtained. Southern hybridization analyses of transformed embryogenic tissue confirmed T-DNA integration.</description><subject>Agrobacterium</subject><subject>Agrobacterium tumefaciens</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cell suspension culture</subject><subject>Cloning</subject><subject>developmental stages</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>gene transfer</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>genetic transformation</subject><subject>Glycine max</subject><subject>methodology</subject><subject>Methods. Procedures. Technologies</subject><subject>Miscellaneous</subject><subject>plant anatomy</subject><subject>Plant tissues</subject><subject>Soybeans</subject><subject>Synthetic digonucleotides and genes. 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Merrill] embryogenic suspension culture tissue</title><author>Trick, H.N ; Finer, J.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-e20fa485d3ef2b2e07d5633d254e49a0e4444daa34b104c7a4622cbec3f8810e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Agrobacterium</topic><topic>Agrobacterium tumefaciens</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cell suspension culture</topic><topic>Cloning</topic><topic>developmental stages</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>gene transfer</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>genetic transformation</topic><topic>Glycine max</topic><topic>methodology</topic><topic>Methods. Procedures. Technologies</topic><topic>Miscellaneous</topic><topic>plant anatomy</topic><topic>Plant tissues</topic><topic>Soybeans</topic><topic>Synthetic digonucleotides and genes. Sequencing</topic><topic>ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trick, H.N</creatorcontrib><creatorcontrib>Finer, J.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Trick, H.N</au><au>Finer, J.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sonication-assisted Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill] embryogenic suspension culture tissue</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>1998-04-01</date><risdate>1998</risdate><volume>17</volume><issue>6/7</issue><spage>482</spage><epage>488</epage><pages>482-488</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Successful transformation of plant tissue using Agrobacterium relies on several factors including bacterial infection, host recognition, and transformation competency of the target tissue. Although soybean [Glycine max (L.) Merrill] embryogenic suspension cultures have been transformed via particle bombardment, Agrobacterium-mediated transformation of this tissue has not been demonstrated. We report here transformation of embryogenic suspension cultures of soybean using "Sonication-Assisted Agrobacterium-mediated Transformation" (SAAT). For SAAT of suspension culture tissue, 10-20 embryogenic clumps (2-4 mm in diameter) were inoculated with 1 ml of diluted (OD600nm 0.1-0.5) log phase Agrobacterium and sonicated for 0-300 s. After 2 days of co-culture in a maintenance medium containing 100 micromolar acetosyringone, the medium was removed and replaced with fresh maintenance medium containing 400 mg/1 Timentin. Two weeks after SAAT, the tissue was placed in maintenance medium containing 20 mg/1 hygromycin and 400 mg/1 Timentin, and the medium was replenished every week thereafter. Transgenic clones were observed and isolated 6-8 weeks following SAAT. When SAAT was not used, hygromycin-resistant clones were not obtained. Southern hybridization analyses of transformed embryogenic tissue confirmed T-DNA integration.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>30736623</pmid><doi>10.1007/s002990050429</doi><tpages>7</tpages></addata></record> |
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| ispartof | Plant cell reports, 1998-04, Vol.17 (6/7), p.482-488 |
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| subjects | Agrobacterium Agrobacterium tumefaciens Bacterial diseases Biological and medical sciences Biotechnology cell suspension culture Cloning developmental stages Fundamental and applied biological sciences. Psychology gene expression gene transfer Genetic engineering Genetic technics genetic transformation Glycine max methodology Methods. Procedures. Technologies Miscellaneous plant anatomy Plant tissues Soybeans Synthetic digonucleotides and genes. Sequencing ultrastructure |
| title | Sonication-assisted Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill] embryogenic suspension culture tissue |
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