Molecular characterization of nucleotide binding and oligomerization domain (NOD)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu ( Labeo rohita)

► Full length cDNA of NOD-2 gene was cloned and characterized in rohu ( Labeo rohita). ► NOD-2 gene expression in embryonic developmental stages was analysed showing its constitutive expression in ontogenesis. ► In healthy rohu, NOD-2 gene was widely expressed in various organs suggesting its role i...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Developmental and comparative immunology 2012, Vol.36 (1), p.93-103
Hauptverfasser: Swain, B., Basu, M., Sahoo, B.R., Maiti, N.K., Routray, P., Eknath, A.E., Samanta, M.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 103
container_issue 1
container_start_page 93
container_title Developmental and comparative immunology
container_volume 36
creator Swain, B.
Basu, M.
Sahoo, B.R.
Maiti, N.K.
Routray, P.
Eknath, A.E.
Samanta, M.
description ► Full length cDNA of NOD-2 gene was cloned and characterized in rohu ( Labeo rohita). ► NOD-2 gene expression in embryonic developmental stages was analysed showing its constitutive expression in ontogenesis. ► In healthy rohu, NOD-2 gene was widely expressed in various organs suggesting its role in immunosurveillance. ► NOD-2 expression in various tissues was modulated by ligands exposure, bacterial infections and poly I:C treatment. ► In vitro and in vivo NOD-2 induction resulted in enhanced expression of RICK and IFN-γ. Nucleotide-binding and oligomerization domain (NOD)-2 is a cytoplasmic pattern recognition receptor (PRR) and is a member of NOD like receptor (NLR) family. It senses a wide range of bacteria and viruses or their products and is involved in innate immune responses. In this report, NOD-2 gene was cloned and characterized from rohu ( Labeo rohita) which is highly commercially important fish species in the Indian subcontinent. The full length rohu NOD-2 (rNOD-2) cDNA comprised of 3176 bp with a single open reading frame (ORF) of 2949 bp encoding a polypeptide of 982 amino acids (aa) with an estimated molecular mass of 109.65 kDa. The rNOD-2 comprised two N-terminal CARD domains (at 4–91 aa and 111–200 aa), one NACHT domain (at 271–441 aa) and seven C-terminal leucine rich repeat (LRR) regions. Phylogenetically, rNOD-2 was closely related to grass carp NOD-2 (gcNOD2) and exhibited significant similarity (94.2%) and identity (88.6%) in their amino acids. Ontogeny analysis of rNOD-2 showed its constitutive expression across the developmental stages, and highlighted the embryonic innate defense system in fish. Tissue specific analysis of rNOD-2 by quantitative real-time PCR (qRT-PCR) revealed its wide distribution; highest expression was in liver followed by blood. In response to PGN and LTA stimulation, Aeromonas hydrophila and Edwardsiella tarda infection, and poly I:C treatment, expression of rNOD-2 and its associated downstream molecules RICK and IFN-γ were significantly enhanced in the treated fish compared to control. These findings suggested the key role of NOD-2 in augmenting innate immunity in fish in response to bacterial and viral infection. This study may be helpful for the development of preventive measures against infectious diseases in fish.
doi_str_mv 10.1016/j.dci.2011.06.018
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_904487466</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0145305X1100187X</els_id><sourcerecordid>904487466</sourcerecordid><originalsourceid>FETCH-LOGICAL-c450t-b2c75f11437b4cd872a59e7c88e9518ed3b800a97f436c86d47050700a72066b3</originalsourceid><addsrcrecordid>eNqNkc9u1DAQxiMEokvhAbgg32glsowTx3bECZW_0kIvIHGzHHuy9cqJt3bSUh6WZ8HpFrghfLHH-n3fjOYriqcU1hQof7lbW-PWFVC6Br4GKu8VKypFWwLI9n6xAsqasobm21HxKKUd5CMpPCyOKiq4aDhbFT8_BY9m9joSc6GjNhNG90NPLowk9GScjccwOYukc6N145bo0ZLg3TYMf0kbBu1GcvL5_M1pWb3IjPY3yaXFwk2JZOlsJneFBL_vI6a0iBYjG67HMk0R9UCS22bZ0qIP3ofr5ZX7ZCwtspDmiLei7jCl9tm3R3M7Qe4ew8VMTshGdxiWwk369HHxoNc-4ZO7-7j4-u7tl7MP5eb8_cez15vSsAamsquMaHpKWS06ZqwUlW5aFEZKbBsq0dadBNCt6FnNjeSWCWhA5C9RAeddfVw8P_juY7icMU1qcMmg93rEMCfVAmNSMM7_gwReN6KtMkkPpIkhpYi92kc36HijKKglf7VTOX-15K-Aq5x_1jy7c5-7Ae0fxe_AM_DqAGDexpXDqJJxOBq0LuZVKhvcP-x_Aa3dxNI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>900635792</pqid></control><display><type>article</type><title>Molecular characterization of nucleotide binding and oligomerization domain (NOD)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu ( Labeo rohita)</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Swain, B. ; Basu, M. ; Sahoo, B.R. ; Maiti, N.K. ; Routray, P. ; Eknath, A.E. ; Samanta, M.</creator><creatorcontrib>Swain, B. ; Basu, M. ; Sahoo, B.R. ; Maiti, N.K. ; Routray, P. ; Eknath, A.E. ; Samanta, M.</creatorcontrib><description>► Full length cDNA of NOD-2 gene was cloned and characterized in rohu ( Labeo rohita). ► NOD-2 gene expression in embryonic developmental stages was analysed showing its constitutive expression in ontogenesis. ► In healthy rohu, NOD-2 gene was widely expressed in various organs suggesting its role in immunosurveillance. ► NOD-2 expression in various tissues was modulated by ligands exposure, bacterial infections and poly I:C treatment. ► In vitro and in vivo NOD-2 induction resulted in enhanced expression of RICK and IFN-γ. Nucleotide-binding and oligomerization domain (NOD)-2 is a cytoplasmic pattern recognition receptor (PRR) and is a member of NOD like receptor (NLR) family. It senses a wide range of bacteria and viruses or their products and is involved in innate immune responses. In this report, NOD-2 gene was cloned and characterized from rohu ( Labeo rohita) which is highly commercially important fish species in the Indian subcontinent. The full length rohu NOD-2 (rNOD-2) cDNA comprised of 3176 bp with a single open reading frame (ORF) of 2949 bp encoding a polypeptide of 982 amino acids (aa) with an estimated molecular mass of 109.65 kDa. The rNOD-2 comprised two N-terminal CARD domains (at 4–91 aa and 111–200 aa), one NACHT domain (at 271–441 aa) and seven C-terminal leucine rich repeat (LRR) regions. Phylogenetically, rNOD-2 was closely related to grass carp NOD-2 (gcNOD2) and exhibited significant similarity (94.2%) and identity (88.6%) in their amino acids. Ontogeny analysis of rNOD-2 showed its constitutive expression across the developmental stages, and highlighted the embryonic innate defense system in fish. Tissue specific analysis of rNOD-2 by quantitative real-time PCR (qRT-PCR) revealed its wide distribution; highest expression was in liver followed by blood. In response to PGN and LTA stimulation, Aeromonas hydrophila and Edwardsiella tarda infection, and poly I:C treatment, expression of rNOD-2 and its associated downstream molecules RICK and IFN-γ were significantly enhanced in the treated fish compared to control. These findings suggested the key role of NOD-2 in augmenting innate immunity in fish in response to bacterial and viral infection. This study may be helpful for the development of preventive measures against infectious diseases in fish.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2011.06.018</identifier><identifier>PMID: 21767564</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Aeromonas hydrophila ; Aeromonas hydrophila - immunology ; Aeromonas hydrophila - pathogenicity ; Amino Acid Sequence ; Animals ; Bacteria ; Carps ; Cells, Cultured ; Cloning, Molecular ; Ctenopharyngodon idella ; Edwardsiella tarda ; Edwardsiella tarda - immunology ; Edwardsiella tarda - pathogenicity ; Evolution, Molecular ; Fish Proteins - genetics ; Fish Proteins - immunology ; Fish Proteins - metabolism ; Gene Expression Profiling ; Gene Expression Regulation - immunology ; Gram-Negative Bacterial Infections - immunology ; IFN-γ ; Immunity, Innate ; Indian major carp ; Labeo rohita ; Ligands ; Molecular Sequence Data ; NOD-2 ; Nod2 Signaling Adaptor Protein - genetics ; Nod2 Signaling Adaptor Protein - immunology ; Nod2 Signaling Adaptor Protein - metabolism ; Phylogeny ; RICK ; Signal Transduction - immunology</subject><ispartof>Developmental and comparative immunology, 2012, Vol.36 (1), p.93-103</ispartof><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-b2c75f11437b4cd872a59e7c88e9518ed3b800a97f436c86d47050700a72066b3</citedby><cites>FETCH-LOGICAL-c450t-b2c75f11437b4cd872a59e7c88e9518ed3b800a97f436c86d47050700a72066b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0145305X1100187X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21767564$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Swain, B.</creatorcontrib><creatorcontrib>Basu, M.</creatorcontrib><creatorcontrib>Sahoo, B.R.</creatorcontrib><creatorcontrib>Maiti, N.K.</creatorcontrib><creatorcontrib>Routray, P.</creatorcontrib><creatorcontrib>Eknath, A.E.</creatorcontrib><creatorcontrib>Samanta, M.</creatorcontrib><title>Molecular characterization of nucleotide binding and oligomerization domain (NOD)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu ( Labeo rohita)</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>► Full length cDNA of NOD-2 gene was cloned and characterized in rohu ( Labeo rohita). ► NOD-2 gene expression in embryonic developmental stages was analysed showing its constitutive expression in ontogenesis. ► In healthy rohu, NOD-2 gene was widely expressed in various organs suggesting its role in immunosurveillance. ► NOD-2 expression in various tissues was modulated by ligands exposure, bacterial infections and poly I:C treatment. ► In vitro and in vivo NOD-2 induction resulted in enhanced expression of RICK and IFN-γ. Nucleotide-binding and oligomerization domain (NOD)-2 is a cytoplasmic pattern recognition receptor (PRR) and is a member of NOD like receptor (NLR) family. It senses a wide range of bacteria and viruses or their products and is involved in innate immune responses. In this report, NOD-2 gene was cloned and characterized from rohu ( Labeo rohita) which is highly commercially important fish species in the Indian subcontinent. The full length rohu NOD-2 (rNOD-2) cDNA comprised of 3176 bp with a single open reading frame (ORF) of 2949 bp encoding a polypeptide of 982 amino acids (aa) with an estimated molecular mass of 109.65 kDa. The rNOD-2 comprised two N-terminal CARD domains (at 4–91 aa and 111–200 aa), one NACHT domain (at 271–441 aa) and seven C-terminal leucine rich repeat (LRR) regions. Phylogenetically, rNOD-2 was closely related to grass carp NOD-2 (gcNOD2) and exhibited significant similarity (94.2%) and identity (88.6%) in their amino acids. Ontogeny analysis of rNOD-2 showed its constitutive expression across the developmental stages, and highlighted the embryonic innate defense system in fish. Tissue specific analysis of rNOD-2 by quantitative real-time PCR (qRT-PCR) revealed its wide distribution; highest expression was in liver followed by blood. In response to PGN and LTA stimulation, Aeromonas hydrophila and Edwardsiella tarda infection, and poly I:C treatment, expression of rNOD-2 and its associated downstream molecules RICK and IFN-γ were significantly enhanced in the treated fish compared to control. These findings suggested the key role of NOD-2 in augmenting innate immunity in fish in response to bacterial and viral infection. This study may be helpful for the development of preventive measures against infectious diseases in fish.</description><subject>Aeromonas hydrophila</subject><subject>Aeromonas hydrophila - immunology</subject><subject>Aeromonas hydrophila - pathogenicity</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Carps</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>Ctenopharyngodon idella</subject><subject>Edwardsiella tarda</subject><subject>Edwardsiella tarda - immunology</subject><subject>Edwardsiella tarda - pathogenicity</subject><subject>Evolution, Molecular</subject><subject>Fish Proteins - genetics</subject><subject>Fish Proteins - immunology</subject><subject>Fish Proteins - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation - immunology</subject><subject>Gram-Negative Bacterial Infections - immunology</subject><subject>IFN-γ</subject><subject>Immunity, Innate</subject><subject>Indian major carp</subject><subject>Labeo rohita</subject><subject>Ligands</subject><subject>Molecular Sequence Data</subject><subject>NOD-2</subject><subject>Nod2 Signaling Adaptor Protein - genetics</subject><subject>Nod2 Signaling Adaptor Protein - immunology</subject><subject>Nod2 Signaling Adaptor Protein - metabolism</subject><subject>Phylogeny</subject><subject>RICK</subject><subject>Signal Transduction - immunology</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQxiMEokvhAbgg32glsowTx3bECZW_0kIvIHGzHHuy9cqJt3bSUh6WZ8HpFrghfLHH-n3fjOYriqcU1hQof7lbW-PWFVC6Br4GKu8VKypFWwLI9n6xAsqasobm21HxKKUd5CMpPCyOKiq4aDhbFT8_BY9m9joSc6GjNhNG90NPLowk9GScjccwOYukc6N145bo0ZLg3TYMf0kbBu1GcvL5_M1pWb3IjPY3yaXFwk2JZOlsJneFBL_vI6a0iBYjG67HMk0R9UCS22bZ0qIP3ofr5ZX7ZCwtspDmiLei7jCl9tm3R3M7Qe4ew8VMTshGdxiWwk369HHxoNc-4ZO7-7j4-u7tl7MP5eb8_cez15vSsAamsquMaHpKWS06ZqwUlW5aFEZKbBsq0dadBNCt6FnNjeSWCWhA5C9RAeddfVw8P_juY7icMU1qcMmg93rEMCfVAmNSMM7_gwReN6KtMkkPpIkhpYi92kc36HijKKglf7VTOX-15K-Aq5x_1jy7c5-7Ae0fxe_AM_DqAGDexpXDqJJxOBq0LuZVKhvcP-x_Aa3dxNI</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Swain, B.</creator><creator>Basu, M.</creator><creator>Sahoo, B.R.</creator><creator>Maiti, N.K.</creator><creator>Routray, P.</creator><creator>Eknath, A.E.</creator><creator>Samanta, M.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TN</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>2012</creationdate><title>Molecular characterization of nucleotide binding and oligomerization domain (NOD)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu ( Labeo rohita)</title><author>Swain, B. ; Basu, M. ; Sahoo, B.R. ; Maiti, N.K. ; Routray, P. ; Eknath, A.E. ; Samanta, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-b2c75f11437b4cd872a59e7c88e9518ed3b800a97f436c86d47050700a72066b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aeromonas hydrophila</topic><topic>Aeromonas hydrophila - immunology</topic><topic>Aeromonas hydrophila - pathogenicity</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Carps</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>Ctenopharyngodon idella</topic><topic>Edwardsiella tarda</topic><topic>Edwardsiella tarda - immunology</topic><topic>Edwardsiella tarda - pathogenicity</topic><topic>Evolution, Molecular</topic><topic>Fish Proteins - genetics</topic><topic>Fish Proteins - immunology</topic><topic>Fish Proteins - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation - immunology</topic><topic>Gram-Negative Bacterial Infections - immunology</topic><topic>IFN-γ</topic><topic>Immunity, Innate</topic><topic>Indian major carp</topic><topic>Labeo rohita</topic><topic>Ligands</topic><topic>Molecular Sequence Data</topic><topic>NOD-2</topic><topic>Nod2 Signaling Adaptor Protein - genetics</topic><topic>Nod2 Signaling Adaptor Protein - immunology</topic><topic>Nod2 Signaling Adaptor Protein - metabolism</topic><topic>Phylogeny</topic><topic>RICK</topic><topic>Signal Transduction - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Swain, B.</creatorcontrib><creatorcontrib>Basu, M.</creatorcontrib><creatorcontrib>Sahoo, B.R.</creatorcontrib><creatorcontrib>Maiti, N.K.</creatorcontrib><creatorcontrib>Routray, P.</creatorcontrib><creatorcontrib>Eknath, A.E.</creatorcontrib><creatorcontrib>Samanta, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Swain, B.</au><au>Basu, M.</au><au>Sahoo, B.R.</au><au>Maiti, N.K.</au><au>Routray, P.</au><au>Eknath, A.E.</au><au>Samanta, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of nucleotide binding and oligomerization domain (NOD)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu ( Labeo rohita)</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2012</date><risdate>2012</risdate><volume>36</volume><issue>1</issue><spage>93</spage><epage>103</epage><pages>93-103</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>► Full length cDNA of NOD-2 gene was cloned and characterized in rohu ( Labeo rohita). ► NOD-2 gene expression in embryonic developmental stages was analysed showing its constitutive expression in ontogenesis. ► In healthy rohu, NOD-2 gene was widely expressed in various organs suggesting its role in immunosurveillance. ► NOD-2 expression in various tissues was modulated by ligands exposure, bacterial infections and poly I:C treatment. ► In vitro and in vivo NOD-2 induction resulted in enhanced expression of RICK and IFN-γ. Nucleotide-binding and oligomerization domain (NOD)-2 is a cytoplasmic pattern recognition receptor (PRR) and is a member of NOD like receptor (NLR) family. It senses a wide range of bacteria and viruses or their products and is involved in innate immune responses. In this report, NOD-2 gene was cloned and characterized from rohu ( Labeo rohita) which is highly commercially important fish species in the Indian subcontinent. The full length rohu NOD-2 (rNOD-2) cDNA comprised of 3176 bp with a single open reading frame (ORF) of 2949 bp encoding a polypeptide of 982 amino acids (aa) with an estimated molecular mass of 109.65 kDa. The rNOD-2 comprised two N-terminal CARD domains (at 4–91 aa and 111–200 aa), one NACHT domain (at 271–441 aa) and seven C-terminal leucine rich repeat (LRR) regions. Phylogenetically, rNOD-2 was closely related to grass carp NOD-2 (gcNOD2) and exhibited significant similarity (94.2%) and identity (88.6%) in their amino acids. Ontogeny analysis of rNOD-2 showed its constitutive expression across the developmental stages, and highlighted the embryonic innate defense system in fish. Tissue specific analysis of rNOD-2 by quantitative real-time PCR (qRT-PCR) revealed its wide distribution; highest expression was in liver followed by blood. In response to PGN and LTA stimulation, Aeromonas hydrophila and Edwardsiella tarda infection, and poly I:C treatment, expression of rNOD-2 and its associated downstream molecules RICK and IFN-γ were significantly enhanced in the treated fish compared to control. These findings suggested the key role of NOD-2 in augmenting innate immunity in fish in response to bacterial and viral infection. This study may be helpful for the development of preventive measures against infectious diseases in fish.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>21767564</pmid><doi>10.1016/j.dci.2011.06.018</doi><tpages>11</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0145-305X
ispartof Developmental and comparative immunology, 2012, Vol.36 (1), p.93-103
issn 0145-305X
1879-0089
language eng
recordid cdi_proquest_miscellaneous_904487466
source MEDLINE; Elsevier ScienceDirect Journals
subjects Aeromonas hydrophila
Aeromonas hydrophila - immunology
Aeromonas hydrophila - pathogenicity
Amino Acid Sequence
Animals
Bacteria
Carps
Cells, Cultured
Cloning, Molecular
Ctenopharyngodon idella
Edwardsiella tarda
Edwardsiella tarda - immunology
Edwardsiella tarda - pathogenicity
Evolution, Molecular
Fish Proteins - genetics
Fish Proteins - immunology
Fish Proteins - metabolism
Gene Expression Profiling
Gene Expression Regulation - immunology
Gram-Negative Bacterial Infections - immunology
IFN-γ
Immunity, Innate
Indian major carp
Labeo rohita
Ligands
Molecular Sequence Data
NOD-2
Nod2 Signaling Adaptor Protein - genetics
Nod2 Signaling Adaptor Protein - immunology
Nod2 Signaling Adaptor Protein - metabolism
Phylogeny
RICK
Signal Transduction - immunology
title Molecular characterization of nucleotide binding and oligomerization domain (NOD)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu ( Labeo rohita)
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T00%3A07%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20characterization%20of%20nucleotide%20binding%20and%20oligomerization%20domain%20(NOD)-2,%20analysis%20of%20its%20inductive%20expression%20and%20down-stream%20signaling%20following%20ligands%20exposure%20and%20bacterial%20infection%20in%20rohu%20(%20Labeo%20rohita)&rft.jtitle=Developmental%20and%20comparative%20immunology&rft.au=Swain,%20B.&rft.date=2012&rft.volume=36&rft.issue=1&rft.spage=93&rft.epage=103&rft.pages=93-103&rft.issn=0145-305X&rft.eissn=1879-0089&rft_id=info:doi/10.1016/j.dci.2011.06.018&rft_dat=%3Cproquest_cross%3E904487466%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=900635792&rft_id=info:pmid/21767564&rft_els_id=S0145305X1100187X&rfr_iscdi=true