Electrochemical immunosensor detection of antigliadin antibodies from real human serum
► Robust electrochemical immunosensor for detection of human coeliac disease related antibodies. ► Good performace in real human serum media with little non-specific binding. ► Semi-quantitative results matching commercial ELISA kits. The determination of antigliadin antibodies from human serum samp...
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| Veröffentlicht in: | Biosensors & bioelectronics 2011-07, Vol.26 (11), p.4471-4476 |
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| creator | Rosales-Rivera, L.C. Acero-Sánchez, J.L. Lozano-Sánchez, P. Katakis, I. O'Sullivan, C.K. |
| description | ► Robust electrochemical immunosensor for detection of human coeliac disease related antibodies. ► Good performace in real human serum media with little non-specific binding. ► Semi-quantitative results matching commercial ELISA kits.
The determination of antigliadin antibodies from human serum samples is of vital importance for the diagnosis of an autoimmune disease such as celiac disease. An electrochemical immunosensor that mimics traditional ELISA type architecture has been constructed for the detection of antigliadin antibodies with control over the orientation and packing of gliadin antigen molecules on the surface of gold electrodes. The orientation of the antigen on the surface has been achieved using a carboxylic-ended bipodal alkanethiol that is covalently linked with amino groups of the antigen protein. The bipodal thiol presents a long poly(ethyleneglycol)-modified chain that acts as an excellent non-specific adsorption barrier. The bipodal nature of the thiol ensured a good spacing and hence good diffusion properties of electroactive species through the self-assembled monolayer, which is vital for the efficiency of the constructed electrochemical immunosensor.
The electrochemical immunosensor was characterized using surface plasmon resonance as well as electrochemical impedance spectroscopy. Amperometric evaluation of the sensor with polyclonal antigliadin antibodies showed stable and reproducible low limits of detection (46
ng/mL; % RSD
=
8.2,
n
=
5). The behaviour and performance of the electrochemical immunosensor with more complex matrixes such as reference serum solutions and real patient samples was evaluated and compared with commercial ELISA kits demonstrating an excellent degree of correlation in thirty minutes total assay time; the electrochemical immunosensor not only delivers a positive or negative result, it allows the estimation of semi-quantitative antibody contents based on the comparison against clinical reference solutions. |
| doi_str_mv | 10.1016/j.bios.2011.05.004 |
| format | Article |
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The determination of antigliadin antibodies from human serum samples is of vital importance for the diagnosis of an autoimmune disease such as celiac disease. An electrochemical immunosensor that mimics traditional ELISA type architecture has been constructed for the detection of antigliadin antibodies with control over the orientation and packing of gliadin antigen molecules on the surface of gold electrodes. The orientation of the antigen on the surface has been achieved using a carboxylic-ended bipodal alkanethiol that is covalently linked with amino groups of the antigen protein. The bipodal thiol presents a long poly(ethyleneglycol)-modified chain that acts as an excellent non-specific adsorption barrier. The bipodal nature of the thiol ensured a good spacing and hence good diffusion properties of electroactive species through the self-assembled monolayer, which is vital for the efficiency of the constructed electrochemical immunosensor.
The electrochemical immunosensor was characterized using surface plasmon resonance as well as electrochemical impedance spectroscopy. Amperometric evaluation of the sensor with polyclonal antigliadin antibodies showed stable and reproducible low limits of detection (46
ng/mL; % RSD
=
8.2,
n
=
5). The behaviour and performance of the electrochemical immunosensor with more complex matrixes such as reference serum solutions and real patient samples was evaluated and compared with commercial ELISA kits demonstrating an excellent degree of correlation in thirty minutes total assay time; the electrochemical immunosensor not only delivers a positive or negative result, it allows the estimation of semi-quantitative antibody contents based on the comparison against clinical reference solutions.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2011.05.004</identifier><identifier>PMID: 21664117</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Antigliadin antibodies ; Autoantibodies - blood ; Autoantigens ; Biological and medical sciences ; Biosensing Techniques - methods ; Biosensing Techniques - statistics & numerical data ; Biosensors ; Biotechnology ; Celiac disease ; Celiac Disease - diagnosis ; Celiac Disease - immunology ; Dielectric Spectroscopy ; Electrochemical immunosensors ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Gliadin - immunology ; Humans ; Immunoassay - methods ; Limit of Detection ; Methods. Procedures. Technologies ; Self assembled monolayer (SAM) ; Surface Plasmon Resonance ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2011-07, Vol.26 (11), p.4471-4476</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-352a9858b317ac65f500b2f57d12637e5d260bcd608b263e99c659f94e006cd23</citedby><cites>FETCH-LOGICAL-c417t-352a9858b317ac65f500b2f57d12637e5d260bcd608b263e99c659f94e006cd23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2011.05.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24295148$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21664117$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rosales-Rivera, L.C.</creatorcontrib><creatorcontrib>Acero-Sánchez, J.L.</creatorcontrib><creatorcontrib>Lozano-Sánchez, P.</creatorcontrib><creatorcontrib>Katakis, I.</creatorcontrib><creatorcontrib>O'Sullivan, C.K.</creatorcontrib><title>Electrochemical immunosensor detection of antigliadin antibodies from real human serum</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>► Robust electrochemical immunosensor for detection of human coeliac disease related antibodies. ► Good performace in real human serum media with little non-specific binding. ► Semi-quantitative results matching commercial ELISA kits.
The determination of antigliadin antibodies from human serum samples is of vital importance for the diagnosis of an autoimmune disease such as celiac disease. An electrochemical immunosensor that mimics traditional ELISA type architecture has been constructed for the detection of antigliadin antibodies with control over the orientation and packing of gliadin antigen molecules on the surface of gold electrodes. The orientation of the antigen on the surface has been achieved using a carboxylic-ended bipodal alkanethiol that is covalently linked with amino groups of the antigen protein. The bipodal thiol presents a long poly(ethyleneglycol)-modified chain that acts as an excellent non-specific adsorption barrier. The bipodal nature of the thiol ensured a good spacing and hence good diffusion properties of electroactive species through the self-assembled monolayer, which is vital for the efficiency of the constructed electrochemical immunosensor.
The electrochemical immunosensor was characterized using surface plasmon resonance as well as electrochemical impedance spectroscopy. Amperometric evaluation of the sensor with polyclonal antigliadin antibodies showed stable and reproducible low limits of detection (46
ng/mL; % RSD
=
8.2,
n
=
5). The behaviour and performance of the electrochemical immunosensor with more complex matrixes such as reference serum solutions and real patient samples was evaluated and compared with commercial ELISA kits demonstrating an excellent degree of correlation in thirty minutes total assay time; the electrochemical immunosensor not only delivers a positive or negative result, it allows the estimation of semi-quantitative antibody contents based on the comparison against clinical reference solutions.</description><subject>Antigliadin antibodies</subject><subject>Autoantibodies - blood</subject><subject>Autoantigens</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensing Techniques - statistics & numerical data</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Celiac disease</subject><subject>Celiac Disease - diagnosis</subject><subject>Celiac Disease - immunology</subject><subject>Dielectric Spectroscopy</subject><subject>Electrochemical immunosensors</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gliadin - immunology</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Limit of Detection</subject><subject>Methods. Procedures. Technologies</subject><subject>Self assembled monolayer (SAM)</subject><subject>Surface Plasmon Resonance</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vFSEUhomxsdfqH3BhZmNczfTAADMkbkxTP5Im3VS3hIEzlpsBKsyY9N_L9V51pysI53nfnDwQ8opCR4HKy303-VQ6BpR2IDoA_oTs6Dj0LWe9eEp2oIRshZT9OXleyh4ABqrgGTlnVEpO6bAjX68XtGtO9h6Dt2ZpfAhbTAVjSblxuNapT7FJc2Pi6r8t3jgff92n5DyWZs4pNBlr9H4LJjYF8xZekLPZLAVfns4L8uXD9d3Vp_bm9uPnq_c3reV0WNteMKNGMU49HYyVYhYAE5vF4CiT_YDCMQmTdRLGqT6gUhVSs-IIIK1j_QV5e-x9yOn7hmXVwReLy2Iipq1oBZxXIeL_ZKW44qOklWRH0uZUSsZZP2QfTH7UFPRBvN7rg3h9EK9B6Cq-hl6f6rcpoPsT-W26Am9OgCnV85xNtL785ThTgvKxcu-OHFZtPzxmXazHaNH5XP9Cu-T_tcdPHfahNw</recordid><startdate>20110715</startdate><enddate>20110715</enddate><creator>Rosales-Rivera, L.C.</creator><creator>Acero-Sánchez, J.L.</creator><creator>Lozano-Sánchez, P.</creator><creator>Katakis, I.</creator><creator>O'Sullivan, C.K.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110715</creationdate><title>Electrochemical immunosensor detection of antigliadin antibodies from real human serum</title><author>Rosales-Rivera, L.C. ; Acero-Sánchez, J.L. ; Lozano-Sánchez, P. ; Katakis, I. ; O'Sullivan, C.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-352a9858b317ac65f500b2f57d12637e5d260bcd608b263e99c659f94e006cd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antigliadin antibodies</topic><topic>Autoantibodies - blood</topic><topic>Autoantigens</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensing Techniques - statistics & numerical data</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Celiac disease</topic><topic>Celiac Disease - diagnosis</topic><topic>Celiac Disease - immunology</topic><topic>Dielectric Spectroscopy</topic><topic>Electrochemical immunosensors</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gliadin - immunology</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Limit of Detection</topic><topic>Methods. Procedures. Technologies</topic><topic>Self assembled monolayer (SAM)</topic><topic>Surface Plasmon Resonance</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosales-Rivera, L.C.</creatorcontrib><creatorcontrib>Acero-Sánchez, J.L.</creatorcontrib><creatorcontrib>Lozano-Sánchez, P.</creatorcontrib><creatorcontrib>Katakis, I.</creatorcontrib><creatorcontrib>O'Sullivan, C.K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosales-Rivera, L.C.</au><au>Acero-Sánchez, J.L.</au><au>Lozano-Sánchez, P.</au><au>Katakis, I.</au><au>O'Sullivan, C.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical immunosensor detection of antigliadin antibodies from real human serum</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2011-07-15</date><risdate>2011</risdate><volume>26</volume><issue>11</issue><spage>4471</spage><epage>4476</epage><pages>4471-4476</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>► Robust electrochemical immunosensor for detection of human coeliac disease related antibodies. ► Good performace in real human serum media with little non-specific binding. ► Semi-quantitative results matching commercial ELISA kits.
The determination of antigliadin antibodies from human serum samples is of vital importance for the diagnosis of an autoimmune disease such as celiac disease. An electrochemical immunosensor that mimics traditional ELISA type architecture has been constructed for the detection of antigliadin antibodies with control over the orientation and packing of gliadin antigen molecules on the surface of gold electrodes. The orientation of the antigen on the surface has been achieved using a carboxylic-ended bipodal alkanethiol that is covalently linked with amino groups of the antigen protein. The bipodal thiol presents a long poly(ethyleneglycol)-modified chain that acts as an excellent non-specific adsorption barrier. The bipodal nature of the thiol ensured a good spacing and hence good diffusion properties of electroactive species through the self-assembled monolayer, which is vital for the efficiency of the constructed electrochemical immunosensor.
The electrochemical immunosensor was characterized using surface plasmon resonance as well as electrochemical impedance spectroscopy. Amperometric evaluation of the sensor with polyclonal antigliadin antibodies showed stable and reproducible low limits of detection (46
ng/mL; % RSD
=
8.2,
n
=
5). The behaviour and performance of the electrochemical immunosensor with more complex matrixes such as reference serum solutions and real patient samples was evaluated and compared with commercial ELISA kits demonstrating an excellent degree of correlation in thirty minutes total assay time; the electrochemical immunosensor not only delivers a positive or negative result, it allows the estimation of semi-quantitative antibody contents based on the comparison against clinical reference solutions.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>21664117</pmid><doi>10.1016/j.bios.2011.05.004</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0956-5663 |
| ispartof | Biosensors & bioelectronics, 2011-07, Vol.26 (11), p.4471-4476 |
| issn | 0956-5663 1873-4235 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Antigliadin antibodies Autoantibodies - blood Autoantigens Biological and medical sciences Biosensing Techniques - methods Biosensing Techniques - statistics & numerical data Biosensors Biotechnology Celiac disease Celiac Disease - diagnosis Celiac Disease - immunology Dielectric Spectroscopy Electrochemical immunosensors Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Gliadin - immunology Humans Immunoassay - methods Limit of Detection Methods. Procedures. Technologies Self assembled monolayer (SAM) Surface Plasmon Resonance Various methods and equipments |
| title | Electrochemical immunosensor detection of antigliadin antibodies from real human serum |
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