Peptides specific for Mycobacterium avium subspecies paratuberculosis infection: diagnostic potential
Mycobacterium avium subspecies paratuberculosis (Map) is the causative agent of Johne's disease (JD). Current serological diagnostic tests for JD are limited by their sensitivity when used in sub-clinical stages of the disease. Our objective was to identify peptides that mimic diagnostically im...
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| Veröffentlicht in: | Protein engineering, design and selection design and selection, 2011-08, Vol.24 (8), p.589-596 |
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| creator | Casey, J.L. Sanalla, A.M. Tamvakis, D. Thalmann, C. Carroll, E.L. Parisi, K. Coley, A.M. Stewart, D.J. Vaughan, J.A. Michalski, W.P. Luke, R. Foley, M. |
| description | Mycobacterium avium subspecies paratuberculosis (Map) is the causative agent of Johne's disease (JD). Current serological diagnostic tests for JD are limited by their sensitivity when used in sub-clinical stages of the disease. Our objective was to identify peptides that mimic diagnostically important Map epitopes that might be incorporated into a new-generation JD diagnostic. Four peptides were isolated from a phage-displayed random peptide library by screening on antibodies derived from Map-infected goats. The peptides were recognised by antibodies from Map-infected goats but not by antibodies from uninfected goats. The peptides elicited immune responses in rabbits, which reacted strongly with bona fide Map antigens proving the peptides were true epitope mimics. To assess the diagnostic value a panel of goat sera was screened for reactivity's with peptides. The peptides were recognised by antibodies from a proportion of goats infected with Map compared with control animals with a diagnostic specificity of 100% and the sensitivity ranged from 50 to 75%. Combinations of any two peptides improved sensitivity 62.5–87.5% and 100% sensitivity was achieved with three of the four peptides in combination. These data suggest peptides representing diagnostically important Map epitopes could be incorporated into a sensitive diagnostic test. |
| doi_str_mv | 10.1093/protein/gzr026 |
| format | Article |
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Current serological diagnostic tests for JD are limited by their sensitivity when used in sub-clinical stages of the disease. Our objective was to identify peptides that mimic diagnostically important Map epitopes that might be incorporated into a new-generation JD diagnostic. Four peptides were isolated from a phage-displayed random peptide library by screening on antibodies derived from Map-infected goats. The peptides were recognised by antibodies from Map-infected goats but not by antibodies from uninfected goats. The peptides elicited immune responses in rabbits, which reacted strongly with bona fide Map antigens proving the peptides were true epitope mimics. To assess the diagnostic value a panel of goat sera was screened for reactivity's with peptides. The peptides were recognised by antibodies from a proportion of goats infected with Map compared with control animals with a diagnostic specificity of 100% and the sensitivity ranged from 50 to 75%. Combinations of any two peptides improved sensitivity 62.5–87.5% and 100% sensitivity was achieved with three of the four peptides in combination. These data suggest peptides representing diagnostically important Map epitopes could be incorporated into a sensitive diagnostic test.</description><identifier>ISSN: 1741-0126</identifier><identifier>EISSN: 1741-0134</identifier><identifier>DOI: 10.1093/protein/gzr026</identifier><identifier>PMID: 21669956</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Bacterial - blood ; Antigens, Bacterial - metabolism ; Bacterial Proteins - metabolism ; Bacterial Typing Techniques - methods ; Biomarkers - blood ; Epitopes ; Goats ; Molecular Sequence Data ; Mycobacterium avium ; Mycobacterium avium subsp. paratuberculosis - isolation & purification ; Mycobacterium avium subsp. paratuberculosis - metabolism ; Paratuberculosis - diagnosis ; Paratuberculosis - microbiology ; Peptide Library ; Peptides - metabolism ; Predictive Value of Tests ; Rabbits ; Serologic Tests - methods</subject><ispartof>Protein engineering, design and selection, 2011-08, Vol.24 (8), p.589-596</ispartof><rights>The Author 2011. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-34ed94b2020ce7c1701b4e8308e0658a60fb8fea41ccffaa5338a88852eb0b6a3</citedby><cites>FETCH-LOGICAL-c360t-34ed94b2020ce7c1701b4e8308e0658a60fb8fea41ccffaa5338a88852eb0b6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21669956$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Casey, J.L.</creatorcontrib><creatorcontrib>Sanalla, A.M.</creatorcontrib><creatorcontrib>Tamvakis, D.</creatorcontrib><creatorcontrib>Thalmann, C.</creatorcontrib><creatorcontrib>Carroll, E.L.</creatorcontrib><creatorcontrib>Parisi, K.</creatorcontrib><creatorcontrib>Coley, A.M.</creatorcontrib><creatorcontrib>Stewart, D.J.</creatorcontrib><creatorcontrib>Vaughan, J.A.</creatorcontrib><creatorcontrib>Michalski, W.P.</creatorcontrib><creatorcontrib>Luke, R.</creatorcontrib><creatorcontrib>Foley, M.</creatorcontrib><title>Peptides specific for Mycobacterium avium subspecies paratuberculosis infection: diagnostic potential</title><title>Protein engineering, design and selection</title><addtitle>Protein Eng Des Sel</addtitle><description>Mycobacterium avium subspecies paratuberculosis (Map) is the causative agent of Johne's disease (JD). Current serological diagnostic tests for JD are limited by their sensitivity when used in sub-clinical stages of the disease. Our objective was to identify peptides that mimic diagnostically important Map epitopes that might be incorporated into a new-generation JD diagnostic. Four peptides were isolated from a phage-displayed random peptide library by screening on antibodies derived from Map-infected goats. The peptides were recognised by antibodies from Map-infected goats but not by antibodies from uninfected goats. The peptides elicited immune responses in rabbits, which reacted strongly with bona fide Map antigens proving the peptides were true epitope mimics. To assess the diagnostic value a panel of goat sera was screened for reactivity's with peptides. The peptides were recognised by antibodies from a proportion of goats infected with Map compared with control animals with a diagnostic specificity of 100% and the sensitivity ranged from 50 to 75%. Combinations of any two peptides improved sensitivity 62.5–87.5% and 100% sensitivity was achieved with three of the four peptides in combination. These data suggest peptides representing diagnostically important Map epitopes could be incorporated into a sensitive diagnostic test.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial - metabolism</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Biomarkers - blood</subject><subject>Epitopes</subject><subject>Goats</subject><subject>Molecular Sequence Data</subject><subject>Mycobacterium avium</subject><subject>Mycobacterium avium subsp. paratuberculosis - isolation & purification</subject><subject>Mycobacterium avium subsp. paratuberculosis - metabolism</subject><subject>Paratuberculosis - diagnosis</subject><subject>Paratuberculosis - microbiology</subject><subject>Peptide Library</subject><subject>Peptides - metabolism</subject><subject>Predictive Value of Tests</subject><subject>Rabbits</subject><subject>Serologic Tests - methods</subject><issn>1741-0126</issn><issn>1741-0134</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c1PwyAYBnBiNG5Orx5Nb8ZDN6Atpd7M4lcyowc9N0BfFkxbKlCT-dfb2bnrLsDh9z4h74PQJcFzgotk0TkbwLSL9Y_DlB2hKclTEmOSpMf7N2UTdOb9Jx5ETsgpmlDCWFFkbIrgDbpgKvCR70AZbVSkrYteNspKoQI40zeR-N6evpd_ZrCdcCL0Epzqa-uNj0yrQQVj29uoMmLdWh-GpG74WxuMqM_RiRa1h4vdPUMfD_fvy6d49fr4vLxbxSphOMRJClWRSoopVpArkmMiU-AJ5oBZxgXDWnINIiVKaS1EliRccM4zChJLJpIZuh5zh7V89eBD2RivoK5FC7b3ZYHTlDNO6UHJc0Z4nlM-yPkolbPeO9Bl50wj3KYkuNx2UO46KMcOhoGrXXQvG6j2_H_pA7gZge27Q2G_leGWPQ</recordid><startdate>201108</startdate><enddate>201108</enddate><creator>Casey, J.L.</creator><creator>Sanalla, A.M.</creator><creator>Tamvakis, D.</creator><creator>Thalmann, C.</creator><creator>Carroll, E.L.</creator><creator>Parisi, K.</creator><creator>Coley, A.M.</creator><creator>Stewart, D.J.</creator><creator>Vaughan, J.A.</creator><creator>Michalski, W.P.</creator><creator>Luke, R.</creator><creator>Foley, M.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201108</creationdate><title>Peptides specific for Mycobacterium avium subspecies paratuberculosis infection: diagnostic potential</title><author>Casey, J.L. ; Sanalla, A.M. ; Tamvakis, D. ; Thalmann, C. ; Carroll, E.L. ; Parisi, K. ; Coley, A.M. ; Stewart, D.J. ; Vaughan, J.A. ; Michalski, W.P. ; Luke, R. ; Foley, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-34ed94b2020ce7c1701b4e8308e0658a60fb8fea41ccffaa5338a88852eb0b6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antigens, Bacterial - metabolism</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Typing Techniques - methods</topic><topic>Biomarkers - blood</topic><topic>Epitopes</topic><topic>Goats</topic><topic>Molecular Sequence Data</topic><topic>Mycobacterium avium</topic><topic>Mycobacterium avium subsp. paratuberculosis - isolation & purification</topic><topic>Mycobacterium avium subsp. paratuberculosis - metabolism</topic><topic>Paratuberculosis - diagnosis</topic><topic>Paratuberculosis - microbiology</topic><topic>Peptide Library</topic><topic>Peptides - metabolism</topic><topic>Predictive Value of Tests</topic><topic>Rabbits</topic><topic>Serologic Tests - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casey, J.L.</creatorcontrib><creatorcontrib>Sanalla, A.M.</creatorcontrib><creatorcontrib>Tamvakis, D.</creatorcontrib><creatorcontrib>Thalmann, C.</creatorcontrib><creatorcontrib>Carroll, E.L.</creatorcontrib><creatorcontrib>Parisi, K.</creatorcontrib><creatorcontrib>Coley, A.M.</creatorcontrib><creatorcontrib>Stewart, D.J.</creatorcontrib><creatorcontrib>Vaughan, J.A.</creatorcontrib><creatorcontrib>Michalski, W.P.</creatorcontrib><creatorcontrib>Luke, R.</creatorcontrib><creatorcontrib>Foley, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Protein engineering, design and selection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casey, J.L.</au><au>Sanalla, A.M.</au><au>Tamvakis, D.</au><au>Thalmann, C.</au><au>Carroll, E.L.</au><au>Parisi, K.</au><au>Coley, A.M.</au><au>Stewart, D.J.</au><au>Vaughan, J.A.</au><au>Michalski, W.P.</au><au>Luke, R.</au><au>Foley, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peptides specific for Mycobacterium avium subspecies paratuberculosis infection: diagnostic potential</atitle><jtitle>Protein engineering, design and selection</jtitle><addtitle>Protein Eng Des Sel</addtitle><date>2011-08</date><risdate>2011</risdate><volume>24</volume><issue>8</issue><spage>589</spage><epage>596</epage><pages>589-596</pages><issn>1741-0126</issn><eissn>1741-0134</eissn><abstract>Mycobacterium avium subspecies paratuberculosis (Map) is the causative agent of Johne's disease (JD). Current serological diagnostic tests for JD are limited by their sensitivity when used in sub-clinical stages of the disease. Our objective was to identify peptides that mimic diagnostically important Map epitopes that might be incorporated into a new-generation JD diagnostic. Four peptides were isolated from a phage-displayed random peptide library by screening on antibodies derived from Map-infected goats. The peptides were recognised by antibodies from Map-infected goats but not by antibodies from uninfected goats. The peptides elicited immune responses in rabbits, which reacted strongly with bona fide Map antigens proving the peptides were true epitope mimics. To assess the diagnostic value a panel of goat sera was screened for reactivity's with peptides. The peptides were recognised by antibodies from a proportion of goats infected with Map compared with control animals with a diagnostic specificity of 100% and the sensitivity ranged from 50 to 75%. Combinations of any two peptides improved sensitivity 62.5–87.5% and 100% sensitivity was achieved with three of the four peptides in combination. These data suggest peptides representing diagnostically important Map epitopes could be incorporated into a sensitive diagnostic test.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>21669956</pmid><doi>10.1093/protein/gzr026</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Antibodies, Bacterial - blood Antigens, Bacterial - metabolism Bacterial Proteins - metabolism Bacterial Typing Techniques - methods Biomarkers - blood Epitopes Goats Molecular Sequence Data Mycobacterium avium Mycobacterium avium subsp. paratuberculosis - isolation & purification Mycobacterium avium subsp. paratuberculosis - metabolism Paratuberculosis - diagnosis Paratuberculosis - microbiology Peptide Library Peptides - metabolism Predictive Value of Tests Rabbits Serologic Tests - methods |
| title | Peptides specific for Mycobacterium avium subspecies paratuberculosis infection: diagnostic potential |
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