Viable calves produced by somatic cell nuclear transfer using meiotic-blocked oocytes
Somatic cell nuclear transfer (SCNT) has had an enormous impact on our understanding of biology and remains a unique tool for multiplying valuable laboratory and domestic animals. However, the complexity of the procedure and its poor efficiency are factors that limit a wider application of SCNT. In...
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| Veröffentlicht in: | Cellular reprogramming 2011-10, Vol.13 (5), p.419-429 |
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| creator | De Bem, Tiago H C Chiaratti, Marcos R Rochetti, Raquel Bressan, Fabiana F Sangalli, Juliano R Miranda, Moysés S Pires, Pedro R L Schwartz, Kátia R L Sampaio, Rafael V Fantinato-Neto, Paulo Pimentel, José R V Perecin, Felipe Smith, Lawrence C Meirelles, Flávio V Adona, Paulo R Leal, Cláudia L V |
| description | Somatic cell nuclear transfer (SCNT) has had an enormous impact on our understanding of biology and remains a unique tool for multiplying valuable laboratory and domestic animals. However, the complexity of the procedure and its poor efficiency are factors that limit a wider application of SCNT. In this context, oocyte meiotic arrest is an important option to make SCNT more flexible and increase the number of cloned embryos produced. Herein, we show that the use of butyrolactone I in association with brain-derived neurotrophic factor (BDNF) to arrest the meiotic division for 24 h prior to in vitro maturation provides bovine (Bos indicus) oocytes capable of supporting development of blastocysts and full-term cloned calves at least as efficiently as nonarrested oocytes. Furthermore, the procedure resulted in cloned blastocysts with an 1.5- and twofold increase of POU5F1 and IFNT2 expression, respectively, which are well-known markers of embryonic viability. Mitochondrial DNA (mtDNA) copy number was diminished by prematuration in immature oocytes (718,585±34,775 vs. 595,579±31,922, respectively, control and treated groups) but was unchanged in mature oocytes (522,179±45,617 vs. 498,771±33,231) and blastocysts (816,627±40,235 vs. 765,332±51,104). To our knowledge, this is the first report of cloned offspring born to prematured oocytes, indicating that meiotic arrest could have significant implications for laboratories working with SCNT and in vitro embryo production. |
| doi_str_mv | 10.1089/cell.2011.0010 |
| format | Article |
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However, the complexity of the procedure and its poor efficiency are factors that limit a wider application of SCNT. In this context, oocyte meiotic arrest is an important option to make SCNT more flexible and increase the number of cloned embryos produced. Herein, we show that the use of butyrolactone I in association with brain-derived neurotrophic factor (BDNF) to arrest the meiotic division for 24 h prior to in vitro maturation provides bovine (Bos indicus) oocytes capable of supporting development of blastocysts and full-term cloned calves at least as efficiently as nonarrested oocytes. Furthermore, the procedure resulted in cloned blastocysts with an 1.5- and twofold increase of POU5F1 and IFNT2 expression, respectively, which are well-known markers of embryonic viability. Mitochondrial DNA (mtDNA) copy number was diminished by prematuration in immature oocytes (718,585±34,775 vs. 595,579±31,922, respectively, control and treated groups) but was unchanged in mature oocytes (522,179±45,617 vs. 498,771±33,231) and blastocysts (816,627±40,235 vs. 765,332±51,104). To our knowledge, this is the first report of cloned offspring born to prematured oocytes, indicating that meiotic arrest could have significant implications for laboratories working with SCNT and in vitro embryo production.</description><identifier>ISSN: 2152-4971</identifier><identifier>EISSN: 2152-4998</identifier><identifier>DOI: 10.1089/cell.2011.0010</identifier><identifier>PMID: 21740268</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>4-Butyrolactone - analogs & derivatives ; 4-Butyrolactone - pharmacology ; Animal behavior ; Animals ; Blastocyst - cytology ; Blastocyst - metabolism ; blastocysts ; Bos indicus ; Brain-derived neurotrophic factor ; Brain-Derived Neurotrophic Factor - pharmacology ; Butyrolactone ; Cattle ; Cloning, Organism - methods ; copy number ; Domestic animals ; Embryos ; Female ; Gene Expression Regulation, Developmental - drug effects ; Health aspects ; Interferon Type I - biosynthesis ; Meiosis ; Meiosis - drug effects ; Mitochondrial DNA ; Nuclear Transfer Techniques ; Oct-4 protein ; Octamer Transcription Factor-3 - biosynthesis ; Oocytes ; Oocytes - cytology ; Oocytes - metabolism ; Physiological aspects ; Pregnancy ; Pregnancy Proteins - biosynthesis ; Progeny ; Protein Kinase Inhibitors - pharmacology ; somatic cell nuclear transfer</subject><ispartof>Cellular reprogramming, 2011-10, Vol.13 (5), p.419-429</ispartof><rights>COPYRIGHT 2011 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-10424900041470f00b3d81a6578bb30a429ef7bd20dac91682813621cdc3c13b3</citedby><cites>FETCH-LOGICAL-c393t-10424900041470f00b3d81a6578bb30a429ef7bd20dac91682813621cdc3c13b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21740268$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Bem, Tiago H C</creatorcontrib><creatorcontrib>Chiaratti, Marcos R</creatorcontrib><creatorcontrib>Rochetti, Raquel</creatorcontrib><creatorcontrib>Bressan, Fabiana F</creatorcontrib><creatorcontrib>Sangalli, Juliano R</creatorcontrib><creatorcontrib>Miranda, Moysés S</creatorcontrib><creatorcontrib>Pires, Pedro R L</creatorcontrib><creatorcontrib>Schwartz, Kátia R L</creatorcontrib><creatorcontrib>Sampaio, Rafael V</creatorcontrib><creatorcontrib>Fantinato-Neto, Paulo</creatorcontrib><creatorcontrib>Pimentel, José R V</creatorcontrib><creatorcontrib>Perecin, Felipe</creatorcontrib><creatorcontrib>Smith, Lawrence C</creatorcontrib><creatorcontrib>Meirelles, Flávio V</creatorcontrib><creatorcontrib>Adona, Paulo R</creatorcontrib><creatorcontrib>Leal, Cláudia L V</creatorcontrib><title>Viable calves produced by somatic cell nuclear transfer using meiotic-blocked oocytes</title><title>Cellular reprogramming</title><addtitle>Cell Reprogram</addtitle><description>Somatic cell nuclear transfer (SCNT) has had an enormous impact on our understanding of biology and remains a unique tool for multiplying valuable laboratory and domestic animals. However, the complexity of the procedure and its poor efficiency are factors that limit a wider application of SCNT. In this context, oocyte meiotic arrest is an important option to make SCNT more flexible and increase the number of cloned embryos produced. Herein, we show that the use of butyrolactone I in association with brain-derived neurotrophic factor (BDNF) to arrest the meiotic division for 24 h prior to in vitro maturation provides bovine (Bos indicus) oocytes capable of supporting development of blastocysts and full-term cloned calves at least as efficiently as nonarrested oocytes. Furthermore, the procedure resulted in cloned blastocysts with an 1.5- and twofold increase of POU5F1 and IFNT2 expression, respectively, which are well-known markers of embryonic viability. Mitochondrial DNA (mtDNA) copy number was diminished by prematuration in immature oocytes (718,585±34,775 vs. 595,579±31,922, respectively, control and treated groups) but was unchanged in mature oocytes (522,179±45,617 vs. 498,771±33,231) and blastocysts (816,627±40,235 vs. 765,332±51,104). To our knowledge, this is the first report of cloned offspring born to prematured oocytes, indicating that meiotic arrest could have significant implications for laboratories working with SCNT and in vitro embryo production.</description><subject>4-Butyrolactone - analogs & derivatives</subject><subject>4-Butyrolactone - pharmacology</subject><subject>Animal behavior</subject><subject>Animals</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - metabolism</subject><subject>blastocysts</subject><subject>Bos indicus</subject><subject>Brain-derived neurotrophic factor</subject><subject>Brain-Derived Neurotrophic Factor - pharmacology</subject><subject>Butyrolactone</subject><subject>Cattle</subject><subject>Cloning, Organism - methods</subject><subject>copy number</subject><subject>Domestic animals</subject><subject>Embryos</subject><subject>Female</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Health aspects</subject><subject>Interferon Type I - biosynthesis</subject><subject>Meiosis</subject><subject>Meiosis - drug effects</subject><subject>Mitochondrial DNA</subject><subject>Nuclear Transfer Techniques</subject><subject>Oct-4 protein</subject><subject>Octamer Transcription Factor-3 - biosynthesis</subject><subject>Oocytes</subject><subject>Oocytes - cytology</subject><subject>Oocytes - metabolism</subject><subject>Physiological aspects</subject><subject>Pregnancy</subject><subject>Pregnancy Proteins - biosynthesis</subject><subject>Progeny</subject><subject>Protein Kinase Inhibitors - pharmacology</subject><subject>somatic cell nuclear transfer</subject><issn>2152-4971</issn><issn>2152-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkTtLxTAYhoMoKnpWRwk4OPX45dI2GUW8geCiriFJvx6ibaNNK5x_b4qXyWRICM-bPOEl5ITBmoHSFx67bs2BsTUAgx1yyFnJC6m12v3b1-yArFJ6hTyEyLFqnxxwVkvglTokzy_Bug6pt90nJvo-xmb22FC3pSn2dgqeLq_QYfYd2pFOox1SiyOdUxg2tMcQM1O4Lvq3HIvRbydMx2SvtV3C1c96RJ5vrp-u7oqHx9v7q8uHwgstpoKB5FJnMclkDS2AE41itipr5ZwAK7nGtnYNh8Z6zSrFFRMVZ77xwjPhxBE5_743e3_MmCbTh7T42gHjnIwGKVVZ6jKTZ9_kxnZowtDG_BO_0OaS1yArrpTK1PofKs8G--DjgG3I5_8F_BhTGrE172Po7bg1DMzSkVlszNKRWTrKgdMf49n12Pzhv42IL1uGirk</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>De Bem, Tiago H C</creator><creator>Chiaratti, Marcos R</creator><creator>Rochetti, Raquel</creator><creator>Bressan, Fabiana F</creator><creator>Sangalli, Juliano R</creator><creator>Miranda, Moysés S</creator><creator>Pires, Pedro R L</creator><creator>Schwartz, Kátia R L</creator><creator>Sampaio, Rafael V</creator><creator>Fantinato-Neto, Paulo</creator><creator>Pimentel, José R V</creator><creator>Perecin, Felipe</creator><creator>Smith, Lawrence C</creator><creator>Meirelles, Flávio V</creator><creator>Adona, Paulo R</creator><creator>Leal, Cláudia L V</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20111001</creationdate><title>Viable calves produced by somatic cell nuclear transfer using meiotic-blocked oocytes</title><author>De Bem, Tiago H C ; Chiaratti, Marcos R ; Rochetti, Raquel ; Bressan, Fabiana F ; Sangalli, Juliano R ; Miranda, Moysés S ; Pires, Pedro R L ; Schwartz, Kátia R L ; Sampaio, Rafael V ; Fantinato-Neto, Paulo ; Pimentel, José R V ; Perecin, Felipe ; Smith, Lawrence C ; Meirelles, Flávio V ; Adona, Paulo R ; Leal, Cláudia L V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-10424900041470f00b3d81a6578bb30a429ef7bd20dac91682813621cdc3c13b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>4-Butyrolactone - analogs & derivatives</topic><topic>4-Butyrolactone - pharmacology</topic><topic>Animal behavior</topic><topic>Animals</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - metabolism</topic><topic>blastocysts</topic><topic>Bos indicus</topic><topic>Brain-derived neurotrophic factor</topic><topic>Brain-Derived Neurotrophic Factor - pharmacology</topic><topic>Butyrolactone</topic><topic>Cattle</topic><topic>Cloning, Organism - methods</topic><topic>copy number</topic><topic>Domestic animals</topic><topic>Embryos</topic><topic>Female</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Health aspects</topic><topic>Interferon Type I - biosynthesis</topic><topic>Meiosis</topic><topic>Meiosis - drug effects</topic><topic>Mitochondrial DNA</topic><topic>Nuclear Transfer Techniques</topic><topic>Oct-4 protein</topic><topic>Octamer Transcription Factor-3 - biosynthesis</topic><topic>Oocytes</topic><topic>Oocytes - cytology</topic><topic>Oocytes - metabolism</topic><topic>Physiological aspects</topic><topic>Pregnancy</topic><topic>Pregnancy Proteins - biosynthesis</topic><topic>Progeny</topic><topic>Protein Kinase Inhibitors - pharmacology</topic><topic>somatic cell nuclear transfer</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Bem, Tiago H C</creatorcontrib><creatorcontrib>Chiaratti, Marcos R</creatorcontrib><creatorcontrib>Rochetti, Raquel</creatorcontrib><creatorcontrib>Bressan, Fabiana F</creatorcontrib><creatorcontrib>Sangalli, Juliano R</creatorcontrib><creatorcontrib>Miranda, Moysés S</creatorcontrib><creatorcontrib>Pires, Pedro R L</creatorcontrib><creatorcontrib>Schwartz, Kátia R L</creatorcontrib><creatorcontrib>Sampaio, Rafael V</creatorcontrib><creatorcontrib>Fantinato-Neto, Paulo</creatorcontrib><creatorcontrib>Pimentel, José R V</creatorcontrib><creatorcontrib>Perecin, Felipe</creatorcontrib><creatorcontrib>Smith, Lawrence C</creatorcontrib><creatorcontrib>Meirelles, Flávio V</creatorcontrib><creatorcontrib>Adona, Paulo R</creatorcontrib><creatorcontrib>Leal, Cláudia L V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Cellular reprogramming</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Bem, Tiago H C</au><au>Chiaratti, Marcos R</au><au>Rochetti, Raquel</au><au>Bressan, Fabiana F</au><au>Sangalli, Juliano R</au><au>Miranda, Moysés S</au><au>Pires, Pedro R L</au><au>Schwartz, Kátia R L</au><au>Sampaio, Rafael V</au><au>Fantinato-Neto, Paulo</au><au>Pimentel, José R V</au><au>Perecin, Felipe</au><au>Smith, Lawrence C</au><au>Meirelles, Flávio V</au><au>Adona, Paulo R</au><au>Leal, Cláudia L V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Viable calves produced by somatic cell nuclear transfer using meiotic-blocked oocytes</atitle><jtitle>Cellular reprogramming</jtitle><addtitle>Cell Reprogram</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>13</volume><issue>5</issue><spage>419</spage><epage>429</epage><pages>419-429</pages><issn>2152-4971</issn><eissn>2152-4998</eissn><abstract>Somatic cell nuclear transfer (SCNT) has had an enormous impact on our understanding of biology and remains a unique tool for multiplying valuable laboratory and domestic animals. However, the complexity of the procedure and its poor efficiency are factors that limit a wider application of SCNT. In this context, oocyte meiotic arrest is an important option to make SCNT more flexible and increase the number of cloned embryos produced. Herein, we show that the use of butyrolactone I in association with brain-derived neurotrophic factor (BDNF) to arrest the meiotic division for 24 h prior to in vitro maturation provides bovine (Bos indicus) oocytes capable of supporting development of blastocysts and full-term cloned calves at least as efficiently as nonarrested oocytes. Furthermore, the procedure resulted in cloned blastocysts with an 1.5- and twofold increase of POU5F1 and IFNT2 expression, respectively, which are well-known markers of embryonic viability. Mitochondrial DNA (mtDNA) copy number was diminished by prematuration in immature oocytes (718,585±34,775 vs. 595,579±31,922, respectively, control and treated groups) but was unchanged in mature oocytes (522,179±45,617 vs. 498,771±33,231) and blastocysts (816,627±40,235 vs. 765,332±51,104). To our knowledge, this is the first report of cloned offspring born to prematured oocytes, indicating that meiotic arrest could have significant implications for laboratories working with SCNT and in vitro embryo production.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>21740268</pmid><doi>10.1089/cell.2011.0010</doi><tpages>11</tpages></addata></record> |
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| subjects | 4-Butyrolactone - analogs & derivatives 4-Butyrolactone - pharmacology Animal behavior Animals Blastocyst - cytology Blastocyst - metabolism blastocysts Bos indicus Brain-derived neurotrophic factor Brain-Derived Neurotrophic Factor - pharmacology Butyrolactone Cattle Cloning, Organism - methods copy number Domestic animals Embryos Female Gene Expression Regulation, Developmental - drug effects Health aspects Interferon Type I - biosynthesis Meiosis Meiosis - drug effects Mitochondrial DNA Nuclear Transfer Techniques Oct-4 protein Octamer Transcription Factor-3 - biosynthesis Oocytes Oocytes - cytology Oocytes - metabolism Physiological aspects Pregnancy Pregnancy Proteins - biosynthesis Progeny Protein Kinase Inhibitors - pharmacology somatic cell nuclear transfer |
| title | Viable calves produced by somatic cell nuclear transfer using meiotic-blocked oocytes |
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