Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis
African trypanosomiasis (AT), also known as sleeping sickness in humans and Nagana in animals, is a disease caused by the protozoan parasite Trypanosoma brucei. AT is an extremely debilitating disease in human, cattle, and wild animals, and the treatment is difficult with frequent relapses. This wor...
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| Veröffentlicht in: | Parasitology research (1987) 2009, Vol.105 (5), p.1223-1229, Article 1223 |
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| creator | Silva, Marcelo Sousa Prazeres, Duarte Miguel F Lança, Andreia Atouguia, Jorge Monteiro, Gabriel Amaro |
| description | African trypanosomiasis (AT), also known as sleeping sickness in humans and Nagana in animals, is a disease caused by the protozoan parasite Trypanosoma brucei. AT is an extremely debilitating disease in human, cattle, and wild animals, and the treatment is difficult with frequent relapses. This work shows that BALB-c mice immunized intramuscularly with a single dose (100 μg) of a plasmid DNA encoding the 5'-terminal region of the trans-sialidase (nTSA) gene of T. brucei brucei are able to produce IgG antibodies that bind to the bloodstream form of T. brucei-protein extract and recognize the recombinant nTSA protein, expressed in Escherichia coli. Furthermore, this DNA vaccination process was able to protect 60% of mice submitted to a challenge assay with the infective form of T. brucei brucei parasites. These results demonstrate that a DNA vaccine coding for trans-sialidase from T. brucei is potentially useful in the prophylaxis of AT. |
| doi_str_mv | 10.1007/s00436-009-1542-6 |
| format | Article |
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AT is an extremely debilitating disease in human, cattle, and wild animals, and the treatment is difficult with frequent relapses. This work shows that BALB-c mice immunized intramuscularly with a single dose (100 μg) of a plasmid DNA encoding the 5'-terminal region of the trans-sialidase (nTSA) gene of T. brucei brucei are able to produce IgG antibodies that bind to the bloodstream form of T. brucei-protein extract and recognize the recombinant nTSA protein, expressed in Escherichia coli. Furthermore, this DNA vaccination process was able to protect 60% of mice submitted to a challenge assay with the infective form of T. brucei brucei parasites. These results demonstrate that a DNA vaccine coding for trans-sialidase from T. brucei is potentially useful in the prophylaxis of AT.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-009-1542-6</identifier><identifier>PMID: 19582478</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>African trypanosomiasis ; Animals ; Antibodies, Protozoan - blood ; Antigens, Protozoan - genetics ; Antigens, Protozoan - immunology ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Deoxyribonucleic acid ; DNA ; DNA vaccines ; Escherichia coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Glycoproteins - genetics ; Glycoproteins - immunology ; Immunoglobulin G ; Immunoglobulin G - blood ; Immunology ; Injections, Intramuscular ; Invertebrates ; Medical Microbiology ; Mice ; Mice, Inbred BALB C ; Microbiology ; Neuraminidase - genetics ; Neuraminidase - immunology ; Original Paper ; Plasmids ; Prophylaxis ; Protein Binding ; Protozoa ; Protozoan Vaccines - administration & dosage ; Protozoan Vaccines - genetics ; Protozoan Vaccines - immunology ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Survival Analysis ; trans-Sialidase ; Trypanosoma brucei ; Trypanosoma brucei brucei - enzymology ; Trypanosomiasis, African - prevention & control ; Vaccination ; Vaccine development ; Vaccines ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - genetics ; Vaccines, DNA - immunology</subject><ispartof>Parasitology research (1987), 2009, Vol.105 (5), p.1223-1229, Article 1223</ispartof><rights>Springer-Verlag 2009</rights><rights>2009 INIST-CNRS</rights><rights>Springer-Verlag 2009.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-2e3b60f7650612785433af9e04e5daadf21728241026d422e5b69b22d15a3da83</citedby><cites>FETCH-LOGICAL-c499t-2e3b60f7650612785433af9e04e5daadf21728241026d422e5b69b22d15a3da83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-009-1542-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-009-1542-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21984387$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19582478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silva, Marcelo Sousa</creatorcontrib><creatorcontrib>Prazeres, Duarte Miguel F</creatorcontrib><creatorcontrib>Lança, Andreia</creatorcontrib><creatorcontrib>Atouguia, Jorge</creatorcontrib><creatorcontrib>Monteiro, Gabriel Amaro</creatorcontrib><title>Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>African trypanosomiasis (AT), also known as sleeping sickness in humans and Nagana in animals, is a disease caused by the protozoan parasite Trypanosoma brucei. AT is an extremely debilitating disease in human, cattle, and wild animals, and the treatment is difficult with frequent relapses. This work shows that BALB-c mice immunized intramuscularly with a single dose (100 μg) of a plasmid DNA encoding the 5'-terminal region of the trans-sialidase (nTSA) gene of T. brucei brucei are able to produce IgG antibodies that bind to the bloodstream form of T. brucei-protein extract and recognize the recombinant nTSA protein, expressed in Escherichia coli. Furthermore, this DNA vaccination process was able to protect 60% of mice submitted to a challenge assay with the infective form of T. brucei brucei parasites. These results demonstrate that a DNA vaccine coding for trans-sialidase from T. brucei is potentially useful in the prophylaxis of AT.</description><subject>African trypanosomiasis</subject><subject>Animals</subject><subject>Antibodies, Protozoan - blood</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA vaccines</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - immunology</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>Immunology</subject><subject>Injections, Intramuscular</subject><subject>Invertebrates</subject><subject>Medical Microbiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Neuraminidase - genetics</subject><subject>Neuraminidase - immunology</subject><subject>Original Paper</subject><subject>Plasmids</subject><subject>Prophylaxis</subject><subject>Protein Binding</subject><subject>Protozoa</subject><subject>Protozoan Vaccines - administration & dosage</subject><subject>Protozoan Vaccines - genetics</subject><subject>Protozoan Vaccines - immunology</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Survival Analysis</subject><subject>trans-Sialidase</subject><subject>Trypanosoma brucei</subject><subject>Trypanosoma brucei brucei - enzymology</subject><subject>Trypanosomiasis, African - prevention & control</subject><subject>Vaccination</subject><subject>Vaccine development</subject><subject>Vaccines</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - genetics</subject><subject>Vaccines, DNA - immunology</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUhS0EokPhB7ABSwhYBa6fiZej8pQqWDBdWzeJPXKVxIOdVJp_j0cZtRKLrmzpfuf4Hh9CXjP4xADqzxlACl0BmIopySv9hGyYFLxiRqmnZAOm3IExcUFe5HwLwGot5XNyUeYNl3WzIcddwilXOeAQesyO-hRHukvHA04xxxFpm5bOBYqZIj3E2U1zYemMae9m6mOiX35t6R12XZgc7d2dG-JhLBTFPYYpz3TrU-hwovO9acAc8kvyzOOQ3avzeUluvn3dXf2orn9__3m1va46acxccSdaDb7WCjTjdaOkEOiNA-lUj9h7zmpewjDgupecO9Vq03LeM4Wix0Zcko-r7yHFv4vLsx1D7tww4OTikq0BKRvBFBTyw6OkLkvUAlgB3_0H3sYlTSWF5RIEO32_KBRbqS7FnJPz9pDCiOloGdhTf3btz5b-7Kk_q4vmzdl5aUfXPyjOhRXg_RnA3OHgS3tdyPccZ6aRoqkLx1cul9G0d-lhxcdef7uKPEaL-1SMb_7wEhiYbpgyXPwDF3m7cQ</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Silva, Marcelo Sousa</creator><creator>Prazeres, Duarte Miguel F</creator><creator>Lança, Andreia</creator><creator>Atouguia, Jorge</creator><creator>Monteiro, Gabriel Amaro</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7TM</scope><scope>H94</scope><scope>M7N</scope></search><sort><creationdate>2009</creationdate><title>Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis</title><author>Silva, Marcelo Sousa ; Prazeres, Duarte Miguel F ; Lança, Andreia ; Atouguia, Jorge ; Monteiro, Gabriel Amaro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-2e3b60f7650612785433af9e04e5daadf21728241026d422e5b69b22d15a3da83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>African trypanosomiasis</topic><topic>Animals</topic><topic>Antibodies, Protozoan - blood</topic><topic>Antigens, Protozoan - genetics</topic><topic>Antigens, Protozoan - immunology</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA vaccines</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - immunology</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - blood</topic><topic>Immunology</topic><topic>Injections, Intramuscular</topic><topic>Invertebrates</topic><topic>Medical Microbiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Neuraminidase - genetics</topic><topic>Neuraminidase - immunology</topic><topic>Original Paper</topic><topic>Plasmids</topic><topic>Prophylaxis</topic><topic>Protein Binding</topic><topic>Protozoa</topic><topic>Protozoan Vaccines - administration & dosage</topic><topic>Protozoan Vaccines - genetics</topic><topic>Protozoan Vaccines - immunology</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Survival Analysis</topic><topic>trans-Sialidase</topic><topic>Trypanosoma brucei</topic><topic>Trypanosoma brucei brucei - enzymology</topic><topic>Trypanosomiasis, African - prevention & control</topic><topic>Vaccination</topic><topic>Vaccine development</topic><topic>Vaccines</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - genetics</topic><topic>Vaccines, DNA - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silva, Marcelo Sousa</creatorcontrib><creatorcontrib>Prazeres, Duarte Miguel F</creatorcontrib><creatorcontrib>Lança, Andreia</creatorcontrib><creatorcontrib>Atouguia, Jorge</creatorcontrib><creatorcontrib>Monteiro, Gabriel Amaro</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silva, Marcelo Sousa</au><au>Prazeres, Duarte Miguel F</au><au>Lança, Andreia</au><au>Atouguia, Jorge</au><au>Monteiro, Gabriel Amaro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2009</date><risdate>2009</risdate><volume>105</volume><issue>5</issue><spage>1223</spage><epage>1229</epage><pages>1223-1229</pages><artnum>1223</artnum><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>African trypanosomiasis (AT), also known as sleeping sickness in humans and Nagana in animals, is a disease caused by the protozoan parasite Trypanosoma brucei. AT is an extremely debilitating disease in human, cattle, and wild animals, and the treatment is difficult with frequent relapses. This work shows that BALB-c mice immunized intramuscularly with a single dose (100 μg) of a plasmid DNA encoding the 5'-terminal region of the trans-sialidase (nTSA) gene of T. brucei brucei are able to produce IgG antibodies that bind to the bloodstream form of T. brucei-protein extract and recognize the recombinant nTSA protein, expressed in Escherichia coli. Furthermore, this DNA vaccination process was able to protect 60% of mice submitted to a challenge assay with the infective form of T. brucei brucei parasites. These results demonstrate that a DNA vaccine coding for trans-sialidase from T. brucei is potentially useful in the prophylaxis of AT.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19582478</pmid><doi>10.1007/s00436-009-1542-6</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Parasitology research (1987), 2009, Vol.105 (5), p.1223-1229, Article 1223 |
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| subjects | African trypanosomiasis Animals Antibodies, Protozoan - blood Antigens, Protozoan - genetics Antigens, Protozoan - immunology Biological and medical sciences Biomedical and Life Sciences Biomedicine Deoxyribonucleic acid DNA DNA vaccines Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Glycoproteins - genetics Glycoproteins - immunology Immunoglobulin G Immunoglobulin G - blood Immunology Injections, Intramuscular Invertebrates Medical Microbiology Mice Mice, Inbred BALB C Microbiology Neuraminidase - genetics Neuraminidase - immunology Original Paper Plasmids Prophylaxis Protein Binding Protozoa Protozoan Vaccines - administration & dosage Protozoan Vaccines - genetics Protozoan Vaccines - immunology Recombinant Proteins - genetics Recombinant Proteins - immunology Survival Analysis trans-Sialidase Trypanosoma brucei Trypanosoma brucei brucei - enzymology Trypanosomiasis, African - prevention & control Vaccination Vaccine development Vaccines Vaccines, DNA - administration & dosage Vaccines, DNA - genetics Vaccines, DNA - immunology |
| title | Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis |
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