Methicillin-susceptible Staphylococcus aureus from clinical and community sources are genetically diverse
Abstract Despite the association of methicillin-susceptible S. aureus (MSSA) with several life-threatening diseases, relatively little is known about their clinical epidemiology in Malaysia. We characterized MSSA isolates ( n = 252) obtained from clinical and community (carriage) sources based on sp...
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| Veröffentlicht in: | International journal of medical microbiology 2011-04, Vol.301 (4), p.347-353 |
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| creator | Ghasemzadeh-Moghaddam, Hamed Ghaznavi-Rad, Ehsanollah Sekawi, Zamberi Yun-Khoon, Liew Aziz, Mohammad Nazri Hamat, Rukman Awang Melles, Damian C van Belkum, Alex Shamsudin, Mariana Nor Neela, Vasanthakumari |
| description | Abstract Despite the association of methicillin-susceptible S. aureus (MSSA) with several life-threatening diseases, relatively little is known about their clinical epidemiology in Malaysia. We characterized MSSA isolates ( n = 252) obtained from clinical and community (carriage) sources based on spa sequencing and multilocus sequence typing (MLST). The prevalence of several important virulence genes was determined to further define the molecular characteristics of MSSA clones circulating in Malaysia. Among the 142 clinical and 110 community-acquired MSSA isolates, 98 different spa types were identified, corresponding to 8 different spa clonal clusters ( spa -CCs). In addition, MLST analysis revealed 22 sequence types (STs) with 5 singletons corresponding to 12 MLST-CCs. Interestingly, spa -CC084/085 (MLST-CC15) ( p = 0.038), spa -non-founder 2 (MLST-ST188) ( p = 0.002), and spa -CC127 (MLST-CC1) ( p = 0.049) were identified significantly more often among clinical isolates. spa -CC3204 (MLST-CC121) ( p = 0.02) and spa -CC015 (MLST-CC45) ( p = 0.0002) were more common among community isolates. Five dominant MLST-CCs (CC8, CC121, CC1, CC45, and CC5) having clear counterparts among the major MRSA clones were also identified in this study. While the MSSA strains are usually genetically heterogeneous, a relatively high frequency (19/7.5%) of ST188 (t189) strains was found, with 57.8% of these strains carrying the Panton–Valentine leukocidin (PVL). Analysis of additional virulence genes showed a frequency of 36.5% and 36.9% for seg and sei and 0.8% and 6.3% for etb and tst genes, respectively. Arginine catabolic mobile element (ACME) was detected in 4 community isolates only. These represent the first isolates harbouring this gene in an Asian region. In conclusion, MSSA from the Malaysian community and their clinical counterparts are genetically diverse, but certain clones occur more often among clinical isolates than among carriage isolates and vice versa. |
| doi_str_mv | 10.1016/j.ijmm.2010.10.004 |
| format | Article |
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We characterized MSSA isolates ( n = 252) obtained from clinical and community (carriage) sources based on spa sequencing and multilocus sequence typing (MLST). The prevalence of several important virulence genes was determined to further define the molecular characteristics of MSSA clones circulating in Malaysia. Among the 142 clinical and 110 community-acquired MSSA isolates, 98 different spa types were identified, corresponding to 8 different spa clonal clusters ( spa -CCs). In addition, MLST analysis revealed 22 sequence types (STs) with 5 singletons corresponding to 12 MLST-CCs. Interestingly, spa -CC084/085 (MLST-CC15) ( p = 0.038), spa -non-founder 2 (MLST-ST188) ( p = 0.002), and spa -CC127 (MLST-CC1) ( p = 0.049) were identified significantly more often among clinical isolates. spa -CC3204 (MLST-CC121) ( p = 0.02) and spa -CC015 (MLST-CC45) ( p = 0.0002) were more common among community isolates. Five dominant MLST-CCs (CC8, CC121, CC1, CC45, and CC5) having clear counterparts among the major MRSA clones were also identified in this study. While the MSSA strains are usually genetically heterogeneous, a relatively high frequency (19/7.5%) of ST188 (t189) strains was found, with 57.8% of these strains carrying the Panton–Valentine leukocidin (PVL). Analysis of additional virulence genes showed a frequency of 36.5% and 36.9% for seg and sei and 0.8% and 6.3% for etb and tst genes, respectively. Arginine catabolic mobile element (ACME) was detected in 4 community isolates only. These represent the first isolates harbouring this gene in an Asian region. In conclusion, MSSA from the Malaysian community and their clinical counterparts are genetically diverse, but certain clones occur more often among clinical isolates than among carriage isolates and vice versa.</description><identifier>ISSN: 1438-4221</identifier><identifier>EISSN: 1618-0607</identifier><identifier>DOI: 10.1016/j.ijmm.2010.10.004</identifier><identifier>PMID: 21193348</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Bacterial Typing Techniques ; Child ; Child, Preschool ; Cluster Analysis ; Community-Acquired Infections - microbiology ; Community-associated MSSA ; Cross Infection - microbiology ; Female ; Genetic Variation ; Genotype ; Humans ; Infant ; Infectious Disease ; Invasive MSSA ; Malaysia ; Male ; Medical Education ; Methicillin-susceptible Staphylococcus aureus ; Middle Aged ; Molecular Typing ; MRSA ; Staphylococcal Infections - microbiology ; Staphylococcus aureus ; Staphylococcus aureus - classification ; Staphylococcus aureus - isolation & purification ; Virulence Factors - genetics ; Virulent profile of MSSA ; Young Adult</subject><ispartof>International journal of medical microbiology, 2011-04, Vol.301 (4), p.347-353</ispartof><rights>Elsevier GmbH</rights><rights>2010 Elsevier GmbH</rights><rights>Copyright © 2010 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c552t-7061800b94ff7f7283a30f8d57048cd80cf3924a0a0c45ff2862296d6ee462e03</citedby><cites>FETCH-LOGICAL-c552t-7061800b94ff7f7283a30f8d57048cd80cf3924a0a0c45ff2862296d6ee462e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijmm.2010.10.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21193348$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ghasemzadeh-Moghaddam, Hamed</creatorcontrib><creatorcontrib>Ghaznavi-Rad, Ehsanollah</creatorcontrib><creatorcontrib>Sekawi, Zamberi</creatorcontrib><creatorcontrib>Yun-Khoon, Liew</creatorcontrib><creatorcontrib>Aziz, Mohammad Nazri</creatorcontrib><creatorcontrib>Hamat, Rukman Awang</creatorcontrib><creatorcontrib>Melles, Damian C</creatorcontrib><creatorcontrib>van Belkum, Alex</creatorcontrib><creatorcontrib>Shamsudin, Mariana Nor</creatorcontrib><creatorcontrib>Neela, Vasanthakumari</creatorcontrib><title>Methicillin-susceptible Staphylococcus aureus from clinical and community sources are genetically diverse</title><title>International journal of medical microbiology</title><addtitle>Int J Med Microbiol</addtitle><description>Abstract Despite the association of methicillin-susceptible S. aureus (MSSA) with several life-threatening diseases, relatively little is known about their clinical epidemiology in Malaysia. We characterized MSSA isolates ( n = 252) obtained from clinical and community (carriage) sources based on spa sequencing and multilocus sequence typing (MLST). The prevalence of several important virulence genes was determined to further define the molecular characteristics of MSSA clones circulating in Malaysia. Among the 142 clinical and 110 community-acquired MSSA isolates, 98 different spa types were identified, corresponding to 8 different spa clonal clusters ( spa -CCs). In addition, MLST analysis revealed 22 sequence types (STs) with 5 singletons corresponding to 12 MLST-CCs. Interestingly, spa -CC084/085 (MLST-CC15) ( p = 0.038), spa -non-founder 2 (MLST-ST188) ( p = 0.002), and spa -CC127 (MLST-CC1) ( p = 0.049) were identified significantly more often among clinical isolates. spa -CC3204 (MLST-CC121) ( p = 0.02) and spa -CC015 (MLST-CC45) ( p = 0.0002) were more common among community isolates. Five dominant MLST-CCs (CC8, CC121, CC1, CC45, and CC5) having clear counterparts among the major MRSA clones were also identified in this study. While the MSSA strains are usually genetically heterogeneous, a relatively high frequency (19/7.5%) of ST188 (t189) strains was found, with 57.8% of these strains carrying the Panton–Valentine leukocidin (PVL). Analysis of additional virulence genes showed a frequency of 36.5% and 36.9% for seg and sei and 0.8% and 6.3% for etb and tst genes, respectively. Arginine catabolic mobile element (ACME) was detected in 4 community isolates only. These represent the first isolates harbouring this gene in an Asian region. In conclusion, MSSA from the Malaysian community and their clinical counterparts are genetically diverse, but certain clones occur more often among clinical isolates than among carriage isolates and vice versa.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Bacterial Typing Techniques</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cluster Analysis</subject><subject>Community-Acquired Infections - microbiology</subject><subject>Community-associated MSSA</subject><subject>Cross Infection - microbiology</subject><subject>Female</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Humans</subject><subject>Infant</subject><subject>Infectious Disease</subject><subject>Invasive MSSA</subject><subject>Malaysia</subject><subject>Male</subject><subject>Medical Education</subject><subject>Methicillin-susceptible Staphylococcus aureus</subject><subject>Middle Aged</subject><subject>Molecular Typing</subject><subject>MRSA</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - classification</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Virulence Factors - genetics</subject><subject>Virulent profile of MSSA</subject><subject>Young Adult</subject><issn>1438-4221</issn><issn>1618-0607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkktv1TAQhS0EoqXwB1ig7FjlMn7EcSSEhCpeUhGLtmvL1xlTBye-2Eml_HscbsuCRbua0eibI82cQ8hrCjsKVL4bdn4Yxx2Dv4MdgHhCTqmkqgYJ7dPSC65qwRg9IS9yHgCAdVw-JyeM0o5zoU6J_47zjbc-BD_VeckWD7PfB6wuZ3O4WUO00dolV2ZJWIpLcaxsYb01oTJTX9k4jsvk57XKcUkWC5qw-okTzhsT1qr3t5gyviTPnAkZX93VM3L9-dPV-df64seXb-cfL2rbNGyuWygHAOw74VzrWqa44eBU37QglO0VWMc7JgwYsKJxjinJWCd7iSgkQ-Bn5O1R95Di7wXzrEdfzgrBTBiXrDsQQlFF6aOkahRTLQVWSHYkbYo5J3T6kPxo0qop6M0LPejNC715sc2KF2XpzZ38sh-x_7dy__wCvD8CWN5x6zHpbD1OFnuf0M66j_5h_Q__rd8b8wtXzEOxYyqP1lRnpkFfbmnYwkBLDqhgkv8BIVywjw</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Ghasemzadeh-Moghaddam, Hamed</creator><creator>Ghaznavi-Rad, Ehsanollah</creator><creator>Sekawi, Zamberi</creator><creator>Yun-Khoon, Liew</creator><creator>Aziz, Mohammad Nazri</creator><creator>Hamat, Rukman Awang</creator><creator>Melles, Damian C</creator><creator>van Belkum, Alex</creator><creator>Shamsudin, Mariana Nor</creator><creator>Neela, Vasanthakumari</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20110401</creationdate><title>Methicillin-susceptible Staphylococcus aureus from clinical and community sources are genetically diverse</title><author>Ghasemzadeh-Moghaddam, Hamed ; 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We characterized MSSA isolates ( n = 252) obtained from clinical and community (carriage) sources based on spa sequencing and multilocus sequence typing (MLST). The prevalence of several important virulence genes was determined to further define the molecular characteristics of MSSA clones circulating in Malaysia. Among the 142 clinical and 110 community-acquired MSSA isolates, 98 different spa types were identified, corresponding to 8 different spa clonal clusters ( spa -CCs). In addition, MLST analysis revealed 22 sequence types (STs) with 5 singletons corresponding to 12 MLST-CCs. Interestingly, spa -CC084/085 (MLST-CC15) ( p = 0.038), spa -non-founder 2 (MLST-ST188) ( p = 0.002), and spa -CC127 (MLST-CC1) ( p = 0.049) were identified significantly more often among clinical isolates. spa -CC3204 (MLST-CC121) ( p = 0.02) and spa -CC015 (MLST-CC45) ( p = 0.0002) were more common among community isolates. Five dominant MLST-CCs (CC8, CC121, CC1, CC45, and CC5) having clear counterparts among the major MRSA clones were also identified in this study. While the MSSA strains are usually genetically heterogeneous, a relatively high frequency (19/7.5%) of ST188 (t189) strains was found, with 57.8% of these strains carrying the Panton–Valentine leukocidin (PVL). Analysis of additional virulence genes showed a frequency of 36.5% and 36.9% for seg and sei and 0.8% and 6.3% for etb and tst genes, respectively. Arginine catabolic mobile element (ACME) was detected in 4 community isolates only. These represent the first isolates harbouring this gene in an Asian region. In conclusion, MSSA from the Malaysian community and their clinical counterparts are genetically diverse, but certain clones occur more often among clinical isolates than among carriage isolates and vice versa.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>21193348</pmid><doi>10.1016/j.ijmm.2010.10.004</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Aged Aged, 80 and over Bacterial Typing Techniques Child Child, Preschool Cluster Analysis Community-Acquired Infections - microbiology Community-associated MSSA Cross Infection - microbiology Female Genetic Variation Genotype Humans Infant Infectious Disease Invasive MSSA Malaysia Male Medical Education Methicillin-susceptible Staphylococcus aureus Middle Aged Molecular Typing MRSA Staphylococcal Infections - microbiology Staphylococcus aureus Staphylococcus aureus - classification Staphylococcus aureus - isolation & purification Virulence Factors - genetics Virulent profile of MSSA Young Adult |
| title | Methicillin-susceptible Staphylococcus aureus from clinical and community sources are genetically diverse |
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