8-Methoxypsoralen-DNA Adducts in Patients Treated with 8-Methoxypsoralen and Ultraviolet A Light
The combination of 8-methoxypsoralen (8-MOP) plus ultraviolet A light (320–400nm), termed PUVA, is used in the treatment of psoriasis, a hyperproliferative disease of the skin. This treatment results in the formation of specific 8-MOP adducts with cellular DNA. We have previously developed monoclona...
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| Veröffentlicht in: | Journal of investigative dermatology 1989-01, Vol.92 (1), p.59-63 |
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| container_title | Journal of investigative dermatology |
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| creator | Yang, Xiao Yan Gasparro, Francis P. DeLeo, Vincent A. Santella, Regina M. |
| description | The combination of 8-methoxypsoralen (8-MOP) plus ultraviolet A light (320–400nm), termed PUVA, is used in the treatment of psoriasis, a hyperproliferative disease of the skin. This treatment results in the formation of specific 8-MOP adducts with cellular DNA. We have previously developed monoclonal antibodies which recognize these 8-MOP photoadducts. We now report the use of these antibodies in an indirect immunofluorescence technique to study human skin biopsies. Nuclei in 3 of 5 skin biopsies from psoriasis patients undergoing PUVA therapy were positive for adducts. The presence of adducts by immunofluorescence did not correlate with plasma levels of 8-MOP. Enzyme-linked immunosorbent assays, used to determine whether 8-MOP photoadducts could be detected in DNA isolated from the lymphocytes of psoriasis patients after PUVA therapy, were negative. |
| doi_str_mv | 10.1111/1523-1747.ep13071224 |
| format | Article |
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This treatment results in the formation of specific 8-MOP adducts with cellular DNA. We have previously developed monoclonal antibodies which recognize these 8-MOP photoadducts. We now report the use of these antibodies in an indirect immunofluorescence technique to study human skin biopsies. Nuclei in 3 of 5 skin biopsies from psoriasis patients undergoing PUVA therapy were positive for adducts. The presence of adducts by immunofluorescence did not correlate with plasma levels of 8-MOP. Enzyme-linked immunosorbent assays, used to determine whether 8-MOP photoadducts could be detected in DNA isolated from the lymphocytes of psoriasis patients after PUVA therapy, were negative.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep13071224</identifier><identifier>PMID: 2642513</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>Antibodies, Monoclonal ; Biological and medical sciences ; Biopsy ; Chromatography, High Pressure Liquid ; Dermatology ; DNA Damage ; Enzyme-Linked Immunosorbent Assay ; Fluorescent Antibody Technique ; Humans ; Medical sciences ; Methoxsalen - metabolism ; Psoriasis - drug therapy ; Psoriasis - metabolism ; Psoriasis. Parapsoriasis. Lichen ; PUVA Therapy - adverse effects ; Skin - metabolism</subject><ispartof>Journal of investigative dermatology, 1989-01, Vol.92 (1), p.59-63</ispartof><rights>1989 The Society for Investigative Dermatology, Inc</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-37cf3ae22c8e104e1b8478bed1f9063041a4ae65b2794249727b681a4c6541e93</citedby><cites>FETCH-LOGICAL-c440t-37cf3ae22c8e104e1b8478bed1f9063041a4ae65b2794249727b681a4c6541e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,4012,27906,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7080732$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2642513$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Xiao Yan</creatorcontrib><creatorcontrib>Gasparro, Francis P.</creatorcontrib><creatorcontrib>DeLeo, Vincent A.</creatorcontrib><creatorcontrib>Santella, Regina M.</creatorcontrib><title>8-Methoxypsoralen-DNA Adducts in Patients Treated with 8-Methoxypsoralen and Ultraviolet A Light</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>The combination of 8-methoxypsoralen (8-MOP) plus ultraviolet A light (320–400nm), termed PUVA, is used in the treatment of psoriasis, a hyperproliferative disease of the skin. This treatment results in the formation of specific 8-MOP adducts with cellular DNA. We have previously developed monoclonal antibodies which recognize these 8-MOP photoadducts. We now report the use of these antibodies in an indirect immunofluorescence technique to study human skin biopsies. Nuclei in 3 of 5 skin biopsies from psoriasis patients undergoing PUVA therapy were positive for adducts. The presence of adducts by immunofluorescence did not correlate with plasma levels of 8-MOP. Enzyme-linked immunosorbent assays, used to determine whether 8-MOP photoadducts could be detected in DNA isolated from the lymphocytes of psoriasis patients after PUVA therapy, were negative.</description><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dermatology</subject><subject>DNA Damage</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Methoxsalen - metabolism</subject><subject>Psoriasis - drug therapy</subject><subject>Psoriasis - metabolism</subject><subject>Psoriasis. Parapsoriasis. Lichen</subject><subject>PUVA Therapy - adverse effects</subject><subject>Skin - metabolism</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctu1DAUtRCoTAt_AJIXqF2l-HETOxukUR-ANLQsWomdcZwbxiiTTG2ntH9fj2Y0XSD1bnx1z0PWOYR84OyU5_nMSyELrkCd4ppLprgQ8IrM9ufXZMaYEIVg4tdbchjjX8Z4BaU-IAeiAlFyOSO_dfED03J8eFzHMdgeh-L8ak7nbTu5FKkf6E-bPA55vwloE7b0n09L-p-M2qGlt30K9t6PPSY6pwv_Z5nekTed7SO-371H5Pby4ubsW7G4_vr9bL4oHABLhVSukxaFcBo5A-SNBqUbbHlXs0oy4BYsVmUjVA0CaiVUU-l8dFUJHGt5RE62vusw3k0Yk1n56LDv7YDjFE3NAFStQWfm8YtMpTWwut4QYUt0YYwxYGfWwa9seDScmU0FZpO12WRtnivIso87_6lZYbsX7TLP-KcdbqOzfRfs4Hzc0xTTTEnxbDPYNAXc42XFJSiW8S9bHHOq9x6DiS4X5bD1AV0y7ehf_ucTvHiq2A</recordid><startdate>198901</startdate><enddate>198901</enddate><creator>Yang, Xiao Yan</creator><creator>Gasparro, Francis P.</creator><creator>DeLeo, Vincent A.</creator><creator>Santella, Regina M.</creator><general>Elsevier Inc</general><general>Nature Publishing</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>198901</creationdate><title>8-Methoxypsoralen-DNA Adducts in Patients Treated with 8-Methoxypsoralen and Ultraviolet A Light</title><author>Yang, Xiao Yan ; Gasparro, Francis P. ; DeLeo, Vincent A. ; Santella, Regina M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-37cf3ae22c8e104e1b8478bed1f9063041a4ae65b2794249727b681a4c6541e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Biopsy</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dermatology</topic><topic>DNA Damage</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Methoxsalen - metabolism</topic><topic>Psoriasis - drug therapy</topic><topic>Psoriasis - metabolism</topic><topic>Psoriasis. Parapsoriasis. Lichen</topic><topic>PUVA Therapy - adverse effects</topic><topic>Skin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Xiao Yan</creatorcontrib><creatorcontrib>Gasparro, Francis P.</creatorcontrib><creatorcontrib>DeLeo, Vincent A.</creatorcontrib><creatorcontrib>Santella, Regina M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Xiao Yan</au><au>Gasparro, Francis P.</au><au>DeLeo, Vincent A.</au><au>Santella, Regina M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>8-Methoxypsoralen-DNA Adducts in Patients Treated with 8-Methoxypsoralen and Ultraviolet A Light</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1989-01</date><risdate>1989</risdate><volume>92</volume><issue>1</issue><spage>59</spage><epage>63</epage><pages>59-63</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>The combination of 8-methoxypsoralen (8-MOP) plus ultraviolet A light (320–400nm), termed PUVA, is used in the treatment of psoriasis, a hyperproliferative disease of the skin. This treatment results in the formation of specific 8-MOP adducts with cellular DNA. We have previously developed monoclonal antibodies which recognize these 8-MOP photoadducts. We now report the use of these antibodies in an indirect immunofluorescence technique to study human skin biopsies. Nuclei in 3 of 5 skin biopsies from psoriasis patients undergoing PUVA therapy were positive for adducts. The presence of adducts by immunofluorescence did not correlate with plasma levels of 8-MOP. Enzyme-linked immunosorbent assays, used to determine whether 8-MOP photoadducts could be detected in DNA isolated from the lymphocytes of psoriasis patients after PUVA therapy, were negative.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>2642513</pmid><doi>10.1111/1523-1747.ep13071224</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of investigative dermatology, 1989-01, Vol.92 (1), p.59-63 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Antibodies, Monoclonal Biological and medical sciences Biopsy Chromatography, High Pressure Liquid Dermatology DNA Damage Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Humans Medical sciences Methoxsalen - metabolism Psoriasis - drug therapy Psoriasis - metabolism Psoriasis. Parapsoriasis. Lichen PUVA Therapy - adverse effects Skin - metabolism |
| title | 8-Methoxypsoralen-DNA Adducts in Patients Treated with 8-Methoxypsoralen and Ultraviolet A Light |
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