In vitro biofilm formation and bactericidal activities of methicillin-resistant Staphylococcus aureus clones prevalent in Korea
Abstract The purpose of this study was to quantify the biofilm formations of various methicillin-resistant Staphylococcus aureus (MRSA) clones (ST1, ST5, ST72, ST89, ST239, and ST254) and to evaluate the in vitro activities of antibiotics. Of 86 MRSA isolates tested, 44 isolates (51.2 %) formed biof...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2011-05, Vol.70 (1), p.112-118 |
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| creator | Cha, Jeong-Ok Park, Yong-Keun Lee, Yeong Seon Chung, Gyung Tae |
| description | Abstract The purpose of this study was to quantify the biofilm formations of various methicillin-resistant Staphylococcus aureus (MRSA) clones (ST1, ST5, ST72, ST89, ST239, and ST254) and to evaluate the in vitro activities of antibiotics. Of 86 MRSA isolates tested, 44 isolates (51.2 %) formed biofilms, including 8 strong biofilm producers (7 isolates of ST72 and 1 isolate of ST89). The planktonic MICs, minimal bactericidal concentrations, and minimum biofilm eradication concentrations (MBECs) of 6 antibiotics (clindamycin, gentamicin, linezolid, vancomycin, daptomycin, and tigecycline) were measured for 8 MRSA isolates. The planktonic isolates were susceptible to all agents, with daptomycin being the most effective, followed by gentamicin and vancomycin. However, clindamycin, linezolid, and tigecycline showed bacteriostatic activity against most of the isolates. The biofilms were resistant to high concentrations of most drugs. However, the MBEC values of daptomycin and tigecycline were lower overall than that of other antibiotics. Microscopy studies verified that daptomycin was the most effective drug used in adhesive biofilms, while gentamicin and tigecycline exhibited some bactericidal efficacy. Our results demonstrated that ST72 and ST89 form strong biofilms that may cause problems in hospital setting, and daptomycin, gentamicin, and tigecycline may be choice therapeutics against biofilm-mediated S. aureus infections. |
| doi_str_mv | 10.1016/j.diagmicrobio.2010.11.018 |
| format | Article |
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Of 86 MRSA isolates tested, 44 isolates (51.2 %) formed biofilms, including 8 strong biofilm producers (7 isolates of ST72 and 1 isolate of ST89). The planktonic MICs, minimal bactericidal concentrations, and minimum biofilm eradication concentrations (MBECs) of 6 antibiotics (clindamycin, gentamicin, linezolid, vancomycin, daptomycin, and tigecycline) were measured for 8 MRSA isolates. The planktonic isolates were susceptible to all agents, with daptomycin being the most effective, followed by gentamicin and vancomycin. However, clindamycin, linezolid, and tigecycline showed bacteriostatic activity against most of the isolates. The biofilms were resistant to high concentrations of most drugs. However, the MBEC values of daptomycin and tigecycline were lower overall than that of other antibiotics. Microscopy studies verified that daptomycin was the most effective drug used in adhesive biofilms, while gentamicin and tigecycline exhibited some bactericidal efficacy. Our results demonstrated that ST72 and ST89 form strong biofilms that may cause problems in hospital setting, and daptomycin, gentamicin, and tigecycline may be choice therapeutics against biofilm-mediated S. aureus infections.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2010.11.018</identifier><identifier>PMID: 21398072</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Anti-Bacterial Agents - pharmacology ; Bactericidal activity ; Bacteriology ; Biofilms - growth & development ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Humans ; Infectious Disease ; Infectious diseases ; Internal Medicine ; MBC ; Medical sciences ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - growth & development ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; MIC ; Microbial Sensitivity Tests ; Microbial Viability - drug effects ; Microbiology ; Miscellaneous ; MRSA ; Republic of Korea ; Staphylococcal Infections - microbiology ; Staphylococcus aureus</subject><ispartof>Diagnostic microbiology and infectious disease, 2011-05, Vol.70 (1), p.112-118</ispartof><rights>2011</rights><rights>2015 INIST-CNRS</rights><rights>Crown Copyright © 2011. Published by Elsevier Inc. 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Of 86 MRSA isolates tested, 44 isolates (51.2 %) formed biofilms, including 8 strong biofilm producers (7 isolates of ST72 and 1 isolate of ST89). The planktonic MICs, minimal bactericidal concentrations, and minimum biofilm eradication concentrations (MBECs) of 6 antibiotics (clindamycin, gentamicin, linezolid, vancomycin, daptomycin, and tigecycline) were measured for 8 MRSA isolates. The planktonic isolates were susceptible to all agents, with daptomycin being the most effective, followed by gentamicin and vancomycin. However, clindamycin, linezolid, and tigecycline showed bacteriostatic activity against most of the isolates. The biofilms were resistant to high concentrations of most drugs. However, the MBEC values of daptomycin and tigecycline were lower overall than that of other antibiotics. Microscopy studies verified that daptomycin was the most effective drug used in adhesive biofilms, while gentamicin and tigecycline exhibited some bactericidal efficacy. Our results demonstrated that ST72 and ST89 form strong biofilms that may cause problems in hospital setting, and daptomycin, gentamicin, and tigecycline may be choice therapeutics against biofilm-mediated S. aureus infections.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bactericidal activity</subject><subject>Bacteriology</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>MBC</subject><subject>Medical sciences</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - growth & development</subject><subject>Methicillin-Resistant Staphylococcus aureus - isolation & purification</subject><subject>MIC</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbial Viability - drug effects</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>MRSA</subject><subject>Republic of Korea</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus aureus</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2LFDEQhhtR3NnVvyBBEE89ptIfSXsQZF11ccHD7j1k0tVujelkTLoH5uRfN82MH3jRUwL11FtFnhTFc-Br4NC-2q57Ml9GsjFsKKwFXwqw5qAeFCtQsis5l_xhseKyEqVSXXVWnKe05RxEV_PHxZmAqlNcilXx_dqzPU0xsBw1kBvZEOJoJgqeGd-zjbETRrLUG8fynTJMmFgY2IjTfS44R76MmChNxk_sdjK7-4MLNlg7J2bmiPmwLvjctYu4Nw4zRp59ChHNk-LRYFzCp6fzorh7f3V3-bG8-fzh-vLtTWmbVkxlrTZWNSBkgx2qukdZtS1HLnplN6Bq2RgDA0hQvZBKmLoDRFQd1NACtNVF8fIYu4vh24xp0iMli84Zj2FOuuN1LVUF8p-kaqta5OAl8_WRzB5SijjoXaTRxIMGrhdReqv_FKUXURpAZ1G5-dlpzLwZsf_V-tNMBl6cAJOscUM03lL6zdVQSdEu-747cphfb08YdbKE3mJPEe2k-0D_t8-bv2Js9kp58lc8YNqGOfrsR4NOQnN9u3yt5WcB57xpqqb6AXuazrw</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Cha, Jeong-Ok</creator><creator>Park, Yong-Keun</creator><creator>Lee, Yeong Seon</creator><creator>Chung, Gyung Tae</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20110501</creationdate><title>In vitro biofilm formation and bactericidal activities of methicillin-resistant Staphylococcus aureus clones prevalent in Korea</title><author>Cha, Jeong-Ok ; Park, Yong-Keun ; Lee, Yeong Seon ; Chung, Gyung Tae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-48bc851275e9e84de73660e02d8cb18475aa1f1718d2782a491eee8914161163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bactericidal activity</topic><topic>Bacteriology</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Internal Medicine</topic><topic>MBC</topic><topic>Medical sciences</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - growth & development</topic><topic>Methicillin-Resistant Staphylococcus aureus - isolation & purification</topic><topic>MIC</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbial Viability - drug effects</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>MRSA</topic><topic>Republic of Korea</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus aureus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cha, Jeong-Ok</creatorcontrib><creatorcontrib>Park, Yong-Keun</creatorcontrib><creatorcontrib>Lee, Yeong Seon</creatorcontrib><creatorcontrib>Chung, Gyung Tae</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cha, Jeong-Ok</au><au>Park, Yong-Keun</au><au>Lee, Yeong Seon</au><au>Chung, Gyung Tae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro biofilm formation and bactericidal activities of methicillin-resistant Staphylococcus aureus clones prevalent in Korea</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>70</volume><issue>1</issue><spage>112</spage><epage>118</epage><pages>112-118</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>Abstract The purpose of this study was to quantify the biofilm formations of various methicillin-resistant Staphylococcus aureus (MRSA) clones (ST1, ST5, ST72, ST89, ST239, and ST254) and to evaluate the in vitro activities of antibiotics. Of 86 MRSA isolates tested, 44 isolates (51.2 %) formed biofilms, including 8 strong biofilm producers (7 isolates of ST72 and 1 isolate of ST89). The planktonic MICs, minimal bactericidal concentrations, and minimum biofilm eradication concentrations (MBECs) of 6 antibiotics (clindamycin, gentamicin, linezolid, vancomycin, daptomycin, and tigecycline) were measured for 8 MRSA isolates. The planktonic isolates were susceptible to all agents, with daptomycin being the most effective, followed by gentamicin and vancomycin. However, clindamycin, linezolid, and tigecycline showed bacteriostatic activity against most of the isolates. The biofilms were resistant to high concentrations of most drugs. However, the MBEC values of daptomycin and tigecycline were lower overall than that of other antibiotics. Microscopy studies verified that daptomycin was the most effective drug used in adhesive biofilms, while gentamicin and tigecycline exhibited some bactericidal efficacy. 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| ispartof | Diagnostic microbiology and infectious disease, 2011-05, Vol.70 (1), p.112-118 |
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| subjects | Anti-Bacterial Agents - pharmacology Bactericidal activity Bacteriology Biofilms - growth & development Biological and medical sciences Fundamental and applied biological sciences. Psychology Humans Infectious Disease Infectious diseases Internal Medicine MBC Medical sciences Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - growth & development Methicillin-Resistant Staphylococcus aureus - isolation & purification MIC Microbial Sensitivity Tests Microbial Viability - drug effects Microbiology Miscellaneous MRSA Republic of Korea Staphylococcal Infections - microbiology Staphylococcus aureus |
| title | In vitro biofilm formation and bactericidal activities of methicillin-resistant Staphylococcus aureus clones prevalent in Korea |
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