Reconstruction of Alveolar Bone Defects Using Bone Morphogenetic Protein 2 Mediated Rabbit Dental Pulp Stem Cells Seeded on Nano-Hydroxyapatite/Collagen/Poly(L-lactide)
The objective of the present study was to evaluate the capacity of a tissue-engineered bone complex of recombinant human bone morphogenetic protein 2 (rhBMP-2) - mediated dental pulp stem cells (DPSCs) and nano-hydroxyapatite/collagen/poly(L-lactide) (nHAC/PLA) to reconstruct critical-size alveolar...
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| creator | Liu, Hong-Chen E, Ling-Ling Wang, Dong-Sheng Su, Fang Wu, Xia Shi, Zhan-Ping Lv, Yan Wang, Jia-Zhu |
| description | The objective of the present study was to evaluate the capacity of a tissue-engineered bone complex of recombinant human bone morphogenetic protein 2 (rhBMP-2)
-
mediated dental pulp stem cells (DPSCs) and nano-hydroxyapatite/collagen/poly(L-lactide) (nHAC/PLA) to reconstruct critical-size alveolar bone defects in New Zealand rabbit. Autologous DPSCs were isolated from rabbit dental pulp tissue and expanded
ex vivo
to enrich DPSCs numbers, and then their attachment and differentiation capability were evaluated when cultured on the culture plate or nHAC/PLA. The alveolar bone defects were treated with nHAC/PLA, nHAC/PLA+rhBMP-2, nHAC/PLA+DPSCs, nHAC/PLA+DPSCs+rhBMP-2, and autogenous bone (AB) obtained from iliac bone or were left untreated as a control. X-ray and a polychrome sequential fluorescent labeling were performed postoperatively and the animals were sacrificed 12 weeks after operation for histological observation and histomorphometric analysis. Our results showed that DPSCs expressed STRO-1 and vementin, and favored osteogenesis and adipogenesis in conditioned media. DPSCs attached and spread well, and retained their osteogenic phenotypes on nHAC/PLA. The rhBMP-2 could significantly increase protein content, alkaline phosphatase activity/protein, osteocalcin content, and mineral formation of DPSCs cultured on nHAC/PLA. The X-ray graph, the fluorescent, histological observation, and histomorphometric analysis showed that the nHAC/PLA+DPSCs+rhBMP-2 tissue-engineered bone complex had an earlier mineralization and more bone formation inside the scaffold than nHAC/PLA, nHAC/PLA+rhBMP-2, and nHAC/PLA+DPSCs, or even autologous bone. Implanted DPSCs' contribution to new bone was detected through transfected eGFP genes. Our findings indicated that stem cells existed in adult rabbit dental pulp tissue. The rhBMP-2 promoted osteogenic capability of DPSCs as a potential cell source for periodontal bone regeneration. The nHAC/PLA could serve as a good scaffold for autologous DPSC seeding, proliferation, and differentiation. The tissue-engineered bone complex with nHAC/PLA, rhBMP-2, and autologous DPSCs might be a better alternative to autologous bone for the clinical reconstruction of periodontal bone defects. |
| doi_str_mv | 10.1089/ten.tea.2010.0620 |
| format | Article |
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-
mediated dental pulp stem cells (DPSCs) and nano-hydroxyapatite/collagen/poly(L-lactide) (nHAC/PLA) to reconstruct critical-size alveolar bone defects in New Zealand rabbit. Autologous DPSCs were isolated from rabbit dental pulp tissue and expanded
ex vivo
to enrich DPSCs numbers, and then their attachment and differentiation capability were evaluated when cultured on the culture plate or nHAC/PLA. The alveolar bone defects were treated with nHAC/PLA, nHAC/PLA+rhBMP-2, nHAC/PLA+DPSCs, nHAC/PLA+DPSCs+rhBMP-2, and autogenous bone (AB) obtained from iliac bone or were left untreated as a control. X-ray and a polychrome sequential fluorescent labeling were performed postoperatively and the animals were sacrificed 12 weeks after operation for histological observation and histomorphometric analysis. Our results showed that DPSCs expressed STRO-1 and vementin, and favored osteogenesis and adipogenesis in conditioned media. DPSCs attached and spread well, and retained their osteogenic phenotypes on nHAC/PLA. The rhBMP-2 could significantly increase protein content, alkaline phosphatase activity/protein, osteocalcin content, and mineral formation of DPSCs cultured on nHAC/PLA. The X-ray graph, the fluorescent, histological observation, and histomorphometric analysis showed that the nHAC/PLA+DPSCs+rhBMP-2 tissue-engineered bone complex had an earlier mineralization and more bone formation inside the scaffold than nHAC/PLA, nHAC/PLA+rhBMP-2, and nHAC/PLA+DPSCs, or even autologous bone. Implanted DPSCs' contribution to new bone was detected through transfected eGFP genes. Our findings indicated that stem cells existed in adult rabbit dental pulp tissue. The rhBMP-2 promoted osteogenic capability of DPSCs as a potential cell source for periodontal bone regeneration. The nHAC/PLA could serve as a good scaffold for autologous DPSC seeding, proliferation, and differentiation. The tissue-engineered bone complex with nHAC/PLA, rhBMP-2, and autologous DPSCs might be a better alternative to autologous bone for the clinical reconstruction of periodontal bone defects.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2010.0620</identifier><identifier>PMID: 21563858</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Adipogenesis - drug effects ; Alkaline Phosphatase - metabolism ; Alveolar Process - drug effects ; Alveolar Process - pathology ; Alveolar Process - surgery ; Animals ; Bone Morphogenetic Protein 2 - pharmacology ; Bone morphogenetic proteins ; Bones ; Cell Separation ; Cells, Cultured ; Collagen - pharmacology ; Colony-Forming Units Assay ; Dental pulp ; Dental Pulp - cytology ; Dental Pulp - drug effects ; Dental Pulp - ultrastructure ; Durapatite - chemistry ; Female ; Gene Expression Regulation - drug effects ; Genotype & phenotype ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; Humans ; Morphology ; Nanoparticles - chemistry ; Original Articles ; Osteocalcin - metabolism ; Osteogenesis - drug effects ; Physiological aspects ; Polyesters - pharmacology ; Prosthesis Implantation ; Proteins ; Rabbits ; Recombinant Proteins - pharmacology ; Reconstructive Surgical Procedures - methods ; Stem Cell Transplantation ; Stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Stem Cells - ultrastructure ; Tissue engineering ; Transforming Growth Factor beta - pharmacology</subject><ispartof>Tissue engineering. Part A, 2011-10, Vol.17 (19-20), p.2417-2433</ispartof><rights>2011, Mary Ann Liebert, Inc.</rights><rights>Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2011 Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2011, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c588t-71cb6fce8e85b2dcc97a5988eba2f7d46009c17ebfd9ef875dc6bdba63bec6a23</citedby><cites>FETCH-LOGICAL-c588t-71cb6fce8e85b2dcc97a5988eba2f7d46009c17ebfd9ef875dc6bdba63bec6a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21563858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Hong-Chen</creatorcontrib><creatorcontrib>E, Ling-Ling</creatorcontrib><creatorcontrib>Wang, Dong-Sheng</creatorcontrib><creatorcontrib>Su, Fang</creatorcontrib><creatorcontrib>Wu, Xia</creatorcontrib><creatorcontrib>Shi, Zhan-Ping</creatorcontrib><creatorcontrib>Lv, Yan</creatorcontrib><creatorcontrib>Wang, Jia-Zhu</creatorcontrib><title>Reconstruction of Alveolar Bone Defects Using Bone Morphogenetic Protein 2 Mediated Rabbit Dental Pulp Stem Cells Seeded on Nano-Hydroxyapatite/Collagen/Poly(L-lactide)</title><title>Tissue engineering. Part A</title><addtitle>Tissue Eng Part A</addtitle><description>The objective of the present study was to evaluate the capacity of a tissue-engineered bone complex of recombinant human bone morphogenetic protein 2 (rhBMP-2)
-
mediated dental pulp stem cells (DPSCs) and nano-hydroxyapatite/collagen/poly(L-lactide) (nHAC/PLA) to reconstruct critical-size alveolar bone defects in New Zealand rabbit. Autologous DPSCs were isolated from rabbit dental pulp tissue and expanded
ex vivo
to enrich DPSCs numbers, and then their attachment and differentiation capability were evaluated when cultured on the culture plate or nHAC/PLA. The alveolar bone defects were treated with nHAC/PLA, nHAC/PLA+rhBMP-2, nHAC/PLA+DPSCs, nHAC/PLA+DPSCs+rhBMP-2, and autogenous bone (AB) obtained from iliac bone or were left untreated as a control. X-ray and a polychrome sequential fluorescent labeling were performed postoperatively and the animals were sacrificed 12 weeks after operation for histological observation and histomorphometric analysis. Our results showed that DPSCs expressed STRO-1 and vementin, and favored osteogenesis and adipogenesis in conditioned media. DPSCs attached and spread well, and retained their osteogenic phenotypes on nHAC/PLA. The rhBMP-2 could significantly increase protein content, alkaline phosphatase activity/protein, osteocalcin content, and mineral formation of DPSCs cultured on nHAC/PLA. The X-ray graph, the fluorescent, histological observation, and histomorphometric analysis showed that the nHAC/PLA+DPSCs+rhBMP-2 tissue-engineered bone complex had an earlier mineralization and more bone formation inside the scaffold than nHAC/PLA, nHAC/PLA+rhBMP-2, and nHAC/PLA+DPSCs, or even autologous bone. Implanted DPSCs' contribution to new bone was detected through transfected eGFP genes. Our findings indicated that stem cells existed in adult rabbit dental pulp tissue. The rhBMP-2 promoted osteogenic capability of DPSCs as a potential cell source for periodontal bone regeneration. The nHAC/PLA could serve as a good scaffold for autologous DPSC seeding, proliferation, and differentiation. The tissue-engineered bone complex with nHAC/PLA, rhBMP-2, and autologous DPSCs might be a better alternative to autologous bone for the clinical reconstruction of periodontal bone defects.</description><subject>Adipogenesis - drug effects</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Alveolar Process - drug effects</subject><subject>Alveolar Process - pathology</subject><subject>Alveolar Process - surgery</subject><subject>Animals</subject><subject>Bone Morphogenetic Protein 2 - pharmacology</subject><subject>Bone morphogenetic proteins</subject><subject>Bones</subject><subject>Cell Separation</subject><subject>Cells, Cultured</subject><subject>Collagen - pharmacology</subject><subject>Colony-Forming Units Assay</subject><subject>Dental pulp</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - drug effects</subject><subject>Dental Pulp - ultrastructure</subject><subject>Durapatite - chemistry</subject><subject>Female</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genotype & phenotype</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Humans</subject><subject>Morphology</subject><subject>Nanoparticles - chemistry</subject><subject>Original Articles</subject><subject>Osteocalcin - metabolism</subject><subject>Osteogenesis - drug effects</subject><subject>Physiological aspects</subject><subject>Polyesters - pharmacology</subject><subject>Prosthesis Implantation</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Reconstructive Surgical Procedures - methods</subject><subject>Stem Cell Transplantation</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - ultrastructure</subject><subject>Tissue engineering</subject><subject>Transforming Growth Factor beta - pharmacology</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkl2LEzEUhgdR3LX6A7yRoBfqxbTJfCSZy1o_Vuhq2XXBuyEfZ2qWNKlJRuw_8meaoeuCIighJBye980J5y2KxwTPCebdIoGbJxDzCucKphW-U5ySrmZlXbef797eG3JSPIjxGmOKKWP3i5OKtLTmLT8tflyA8i6mMKpkvEN-QEv7DbwVAb3yDtBrGECliK6icdtj6dyH_Re_BQfJKLQJPoFxqELnoI1IoNGFkNKkLHVJWLQZ7R5dJtihFVgb0SWAzlB-7INwvjw76OC_H8ReJJNgsfLWiuy92Hh7eLEurciNaXj5sLg3CBvh0c05K67evvm0OivXH9-9Xy3XpWo5TyUjStJBAQfeykor1THRdpyDFNXAdEMx7hRhIAfdwcBZqxWVWgpaS1BUVPWseH703Qf_dYSY-p2JKjcuHPgx9h1uGsZo0_yT5F3dccwbksmnf5DXfgwuf2OCmqoidZ2hZ0doKyz0xg0-BaEmy35ZsablLc3znBXzv1B5adiZPEkYTK7_JiBHgQo-xgBDvw9mJ8KhJ7ifUtTnFOUt-ilF_ZSirHly0-8od6BvFb9ikwF2BKaycM4akBDSf1j_BL952HA</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Liu, Hong-Chen</creator><creator>E, Ling-Ling</creator><creator>Wang, Dong-Sheng</creator><creator>Su, Fang</creator><creator>Wu, Xia</creator><creator>Shi, Zhan-Ping</creator><creator>Lv, Yan</creator><creator>Wang, Jia-Zhu</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20111001</creationdate><title>Reconstruction of Alveolar Bone Defects Using Bone Morphogenetic Protein 2 Mediated Rabbit Dental Pulp Stem Cells Seeded on Nano-Hydroxyapatite/Collagen/Poly(L-lactide)</title><author>Liu, Hong-Chen ; E, Ling-Ling ; Wang, Dong-Sheng ; Su, Fang ; Wu, Xia ; Shi, Zhan-Ping ; Lv, Yan ; Wang, Jia-Zhu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c588t-71cb6fce8e85b2dcc97a5988eba2f7d46009c17ebfd9ef875dc6bdba63bec6a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adipogenesis - drug effects</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Alveolar Process - drug effects</topic><topic>Alveolar Process - pathology</topic><topic>Alveolar Process - surgery</topic><topic>Animals</topic><topic>Bone Morphogenetic Protein 2 - pharmacology</topic><topic>Bone morphogenetic proteins</topic><topic>Bones</topic><topic>Cell Separation</topic><topic>Cells, Cultured</topic><topic>Collagen - pharmacology</topic><topic>Colony-Forming Units Assay</topic><topic>Dental pulp</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - drug effects</topic><topic>Dental Pulp - ultrastructure</topic><topic>Durapatite - chemistry</topic><topic>Female</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genotype & phenotype</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Humans</topic><topic>Morphology</topic><topic>Nanoparticles - chemistry</topic><topic>Original Articles</topic><topic>Osteocalcin - metabolism</topic><topic>Osteogenesis - drug effects</topic><topic>Physiological aspects</topic><topic>Polyesters - pharmacology</topic><topic>Prosthesis Implantation</topic><topic>Proteins</topic><topic>Rabbits</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Reconstructive Surgical Procedures - methods</topic><topic>Stem Cell Transplantation</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - ultrastructure</topic><topic>Tissue engineering</topic><topic>Transforming Growth Factor beta - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Hong-Chen</creatorcontrib><creatorcontrib>E, Ling-Ling</creatorcontrib><creatorcontrib>Wang, Dong-Sheng</creatorcontrib><creatorcontrib>Su, Fang</creatorcontrib><creatorcontrib>Wu, Xia</creatorcontrib><creatorcontrib>Shi, Zhan-Ping</creatorcontrib><creatorcontrib>Lv, Yan</creatorcontrib><creatorcontrib>Wang, Jia-Zhu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - 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Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Hong-Chen</au><au>E, Ling-Ling</au><au>Wang, Dong-Sheng</au><au>Su, Fang</au><au>Wu, Xia</au><au>Shi, Zhan-Ping</au><au>Lv, Yan</au><au>Wang, Jia-Zhu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reconstruction of Alveolar Bone Defects Using Bone Morphogenetic Protein 2 Mediated Rabbit Dental Pulp Stem Cells Seeded on Nano-Hydroxyapatite/Collagen/Poly(L-lactide)</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>17</volume><issue>19-20</issue><spage>2417</spage><epage>2433</epage><pages>2417-2433</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>The objective of the present study was to evaluate the capacity of a tissue-engineered bone complex of recombinant human bone morphogenetic protein 2 (rhBMP-2)
-
mediated dental pulp stem cells (DPSCs) and nano-hydroxyapatite/collagen/poly(L-lactide) (nHAC/PLA) to reconstruct critical-size alveolar bone defects in New Zealand rabbit. Autologous DPSCs were isolated from rabbit dental pulp tissue and expanded
ex vivo
to enrich DPSCs numbers, and then their attachment and differentiation capability were evaluated when cultured on the culture plate or nHAC/PLA. The alveolar bone defects were treated with nHAC/PLA, nHAC/PLA+rhBMP-2, nHAC/PLA+DPSCs, nHAC/PLA+DPSCs+rhBMP-2, and autogenous bone (AB) obtained from iliac bone or were left untreated as a control. X-ray and a polychrome sequential fluorescent labeling were performed postoperatively and the animals were sacrificed 12 weeks after operation for histological observation and histomorphometric analysis. Our results showed that DPSCs expressed STRO-1 and vementin, and favored osteogenesis and adipogenesis in conditioned media. DPSCs attached and spread well, and retained their osteogenic phenotypes on nHAC/PLA. The rhBMP-2 could significantly increase protein content, alkaline phosphatase activity/protein, osteocalcin content, and mineral formation of DPSCs cultured on nHAC/PLA. The X-ray graph, the fluorescent, histological observation, and histomorphometric analysis showed that the nHAC/PLA+DPSCs+rhBMP-2 tissue-engineered bone complex had an earlier mineralization and more bone formation inside the scaffold than nHAC/PLA, nHAC/PLA+rhBMP-2, and nHAC/PLA+DPSCs, or even autologous bone. Implanted DPSCs' contribution to new bone was detected through transfected eGFP genes. Our findings indicated that stem cells existed in adult rabbit dental pulp tissue. The rhBMP-2 promoted osteogenic capability of DPSCs as a potential cell source for periodontal bone regeneration. The nHAC/PLA could serve as a good scaffold for autologous DPSC seeding, proliferation, and differentiation. The tissue-engineered bone complex with nHAC/PLA, rhBMP-2, and autologous DPSCs might be a better alternative to autologous bone for the clinical reconstruction of periodontal bone defects.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>21563858</pmid><doi>10.1089/ten.tea.2010.0620</doi><tpages>17</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1937-3341 |
| ispartof | Tissue engineering. Part A, 2011-10, Vol.17 (19-20), p.2417-2433 |
| issn | 1937-3341 1937-335X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_904477644 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adipogenesis - drug effects Alkaline Phosphatase - metabolism Alveolar Process - drug effects Alveolar Process - pathology Alveolar Process - surgery Animals Bone Morphogenetic Protein 2 - pharmacology Bone morphogenetic proteins Bones Cell Separation Cells, Cultured Collagen - pharmacology Colony-Forming Units Assay Dental pulp Dental Pulp - cytology Dental Pulp - drug effects Dental Pulp - ultrastructure Durapatite - chemistry Female Gene Expression Regulation - drug effects Genotype & phenotype Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Humans Morphology Nanoparticles - chemistry Original Articles Osteocalcin - metabolism Osteogenesis - drug effects Physiological aspects Polyesters - pharmacology Prosthesis Implantation Proteins Rabbits Recombinant Proteins - pharmacology Reconstructive Surgical Procedures - methods Stem Cell Transplantation Stem cells Stem Cells - cytology Stem Cells - drug effects Stem Cells - ultrastructure Tissue engineering Transforming Growth Factor beta - pharmacology |
| title | Reconstruction of Alveolar Bone Defects Using Bone Morphogenetic Protein 2 Mediated Rabbit Dental Pulp Stem Cells Seeded on Nano-Hydroxyapatite/Collagen/Poly(L-lactide) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T20%3A47%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Reconstruction%20of%20Alveolar%20Bone%20Defects%20Using%20Bone%20Morphogenetic%20Protein%202%20Mediated%20Rabbit%20Dental%20Pulp%20Stem%20Cells%20Seeded%20on%20Nano-Hydroxyapatite/Collagen/Poly(L-lactide)&rft.jtitle=Tissue%20engineering.%20Part%20A&rft.au=Liu,%20Hong-Chen&rft.date=2011-10-01&rft.volume=17&rft.issue=19-20&rft.spage=2417&rft.epage=2433&rft.pages=2417-2433&rft.issn=1937-3341&rft.eissn=1937-335X&rft_id=info:doi/10.1089/ten.tea.2010.0620&rft_dat=%3Cgale_proqu%3EA274585619%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=893422133&rft_id=info:pmid/21563858&rft_galeid=A274585619&rfr_iscdi=true |