A biodegradable in situ injectable hydrogel based on chitosan and oxidized hyaluronic acid for tissue engineering applications
An “in situ” biodegradable gel consisting of chitosan, glycerol phosphate (GP) and oxidized hyaluronic acid (HDA) were synthesised and characterized .This is a two component hydrogel system where chitosan neutralized with GP resulted in instantaneous gelling when combined with HDA. The gels are cyto...
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| Veröffentlicht in: | Carbohydrate polymers 2011-07, Vol.85 (4), p.838-844 |
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| container_title | Carbohydrate polymers |
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| creator | Nair, Sandhya Remya, N.S. Remya, S. Nair, Prabha D. |
| description | An “in situ” biodegradable gel consisting of chitosan, glycerol phosphate (GP) and oxidized hyaluronic acid (HDA) were synthesised and characterized .This is a two component hydrogel system where chitosan neutralized with GP resulted in instantaneous gelling when combined with HDA. The gels are cytocompatible and could be freeze dried to form porous scaffolds. The percentage porosity of the freeze-dried chitosan hyaluronic acid dialdehyde gels (CHDA) increased with increasing oxidation. Fibroblast cells seeded onto CHDA porous scaffolds adhered, proliferated and produced ECM components on the scaffold. Chondrocytes encapsulated in CHDA gels retained their viability and specific phenotypic characteristics. The gel material is hence proposed as a scaffold and encapsulating material for tissue engineering applications. |
| doi_str_mv | 10.1016/j.carbpol.2011.04.004 |
| format | Article |
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The gels are cytocompatible and could be freeze dried to form porous scaffolds. The percentage porosity of the freeze-dried chitosan hyaluronic acid dialdehyde gels (CHDA) increased with increasing oxidation. Fibroblast cells seeded onto CHDA porous scaffolds adhered, proliferated and produced ECM components on the scaffold. Chondrocytes encapsulated in CHDA gels retained their viability and specific phenotypic characteristics. The gel material is hence proposed as a scaffold and encapsulating material for tissue engineering applications.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2011.04.004</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Applied sciences ; Biodegradability ; Biological and medical sciences ; Carbohydrates ; Chitosan ; Chondrocytes ; encapsulation ; Exact sciences and technology ; Extracellular matrix ; Fibroblasts ; freeze drying ; gelation ; gels ; Glycerol ; Hyaluronic acid ; hydrocolloids ; Hydrogels ; Medical sciences ; Natural polymers ; Oxidation ; Phosphate ; phosphates ; Physicochemistry of polymers ; Porosity ; scaffolds ; Starch and polysaccharides ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. 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The gels are cytocompatible and could be freeze dried to form porous scaffolds. The percentage porosity of the freeze-dried chitosan hyaluronic acid dialdehyde gels (CHDA) increased with increasing oxidation. Fibroblast cells seeded onto CHDA porous scaffolds adhered, proliferated and produced ECM components on the scaffold. Chondrocytes encapsulated in CHDA gels retained their viability and specific phenotypic characteristics. The gel material is hence proposed as a scaffold and encapsulating material for tissue engineering applications.</description><subject>Applied sciences</subject><subject>Biodegradability</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates</subject><subject>Chitosan</subject><subject>Chondrocytes</subject><subject>encapsulation</subject><subject>Exact sciences and technology</subject><subject>Extracellular matrix</subject><subject>Fibroblasts</subject><subject>freeze drying</subject><subject>gelation</subject><subject>gels</subject><subject>Glycerol</subject><subject>Hyaluronic acid</subject><subject>hydrocolloids</subject><subject>Hydrogels</subject><subject>Medical sciences</subject><subject>Natural polymers</subject><subject>Oxidation</subject><subject>Phosphate</subject><subject>phosphates</subject><subject>Physicochemistry of polymers</subject><subject>Porosity</subject><subject>scaffolds</subject><subject>Starch and polysaccharides</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. 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Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Tissue engineering</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nair, Sandhya</creatorcontrib><creatorcontrib>Remya, N.S.</creatorcontrib><creatorcontrib>Remya, S.</creatorcontrib><creatorcontrib>Nair, Prabha D.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nair, Sandhya</au><au>Remya, N.S.</au><au>Remya, S.</au><au>Nair, Prabha D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A biodegradable in situ injectable hydrogel based on chitosan and oxidized hyaluronic acid for tissue engineering applications</atitle><jtitle>Carbohydrate polymers</jtitle><date>2011-07-01</date><risdate>2011</risdate><volume>85</volume><issue>4</issue><spage>838</spage><epage>844</epage><pages>838-844</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>An “in situ” biodegradable gel consisting of chitosan, glycerol phosphate (GP) and oxidized hyaluronic acid (HDA) were synthesised and characterized .This is a two component hydrogel system where chitosan neutralized with GP resulted in instantaneous gelling when combined with HDA. The gels are cytocompatible and could be freeze dried to form porous scaffolds. The percentage porosity of the freeze-dried chitosan hyaluronic acid dialdehyde gels (CHDA) increased with increasing oxidation. Fibroblast cells seeded onto CHDA porous scaffolds adhered, proliferated and produced ECM components on the scaffold. Chondrocytes encapsulated in CHDA gels retained their viability and specific phenotypic characteristics. The gel material is hence proposed as a scaffold and encapsulating material for tissue engineering applications.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.carbpol.2011.04.004</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0144-8617 |
| ispartof | Carbohydrate polymers, 2011-07, Vol.85 (4), p.838-844 |
| issn | 0144-8617 1879-1344 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Applied sciences Biodegradability Biological and medical sciences Carbohydrates Chitosan Chondrocytes encapsulation Exact sciences and technology Extracellular matrix Fibroblasts freeze drying gelation gels Glycerol Hyaluronic acid hydrocolloids Hydrogels Medical sciences Natural polymers Oxidation Phosphate phosphates Physicochemistry of polymers Porosity scaffolds Starch and polysaccharides Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Tissue engineering viability |
| title | A biodegradable in situ injectable hydrogel based on chitosan and oxidized hyaluronic acid for tissue engineering applications |
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