Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm
Although interspecies/intergeneric somatic cell nuclear transfer (iSCNT) has been proposed as a tool to produce offspring of endangered species, conflict between donor nucleus and recipient cytoplasm in iSCNT embryos has been identified as an impediment to implementation for agricultural production....
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| Veröffentlicht in: | Animal science journal 2011-04, Vol.82 (2), p.236-243 |
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| creator | Srirattana, Kanokwan Matsukawa, Kazutsugu Akagi, Satoshi Tasai, Mariko Tagami, Takahiro Nirasawa, Keijiro Nagai, Takashi Kanai, Yukio Parnpai, Rangsun Takeda, Kumiko |
| description | Although interspecies/intergeneric somatic cell nuclear transfer (iSCNT) has been proposed as a tool to produce offspring of endangered species, conflict between donor nucleus and recipient cytoplasm in iSCNT embryos has been identified as an impediment to implementation for agricultural production. To investigate the nuclear-mitochondrial interactions on the developmental potential of iSCNT embryos, we analyzed the mtDNA copy numbers in iSCNT embryos reconstructed with water buffalo (swamp type) fibroblasts and bovine enucleated oocytes (buffalo iSCNT). As controls, SCNT embryos were derived from bovine fibroblasts (bovine SCNT). Buffalo iSCNT and bovine SCNT embryos showed similar rates of cleavage and development to the 8-cell stage (P > 0.05). However, buffalo iSCNT embryos did not develop beyond the 16-cell stage. Both bovine and buffalo mtDNA content in buffalo iSCNT embryos was stable throughout the nuclear transfer process, and arrested at the 8- to 16-cell stage (P > 0.05). In bovine SCNT embryos that developed to the blastocyst stage, mtDNA copy number was increased (P < 0.05). In conclusion, both the donor cell and recipient cytoplast mtDNAs of buffalo iSCNT embryos were identified and maintained through the iSCNT process until the 8-16-cell stage. In addition, the copy number of mtDNA per embryo was a useful monitor to investigate nuclear-mitochondrial interactions. |
| doi_str_mv | 10.1111/j.1740-0929.2010.00827.x |
| format | Article |
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To investigate the nuclear-mitochondrial interactions on the developmental potential of iSCNT embryos, we analyzed the mtDNA copy numbers in iSCNT embryos reconstructed with water buffalo (swamp type) fibroblasts and bovine enucleated oocytes (buffalo iSCNT). As controls, SCNT embryos were derived from bovine fibroblasts (bovine SCNT). Buffalo iSCNT and bovine SCNT embryos showed similar rates of cleavage and development to the 8-cell stage (P > 0.05). However, buffalo iSCNT embryos did not develop beyond the 16-cell stage. Both bovine and buffalo mtDNA content in buffalo iSCNT embryos was stable throughout the nuclear transfer process, and arrested at the 8- to 16-cell stage (P > 0.05). In bovine SCNT embryos that developed to the blastocyst stage, mtDNA copy number was increased (P < 0.05). In conclusion, both the donor cell and recipient cytoplast mtDNAs of buffalo iSCNT embryos were identified and maintained through the iSCNT process until the 8-16-cell stage. In addition, the copy number of mtDNA per embryo was a useful monitor to investigate nuclear-mitochondrial interactions.</description><identifier>ISSN: 1344-3941</identifier><identifier>EISSN: 1740-0929</identifier><identifier>DOI: 10.1111/j.1740-0929.2010.00827.x</identifier><identifier>PMID: 21729201</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Animal reproduction ; Animal sciences ; Animals ; blastocyst ; blastocysts ; bovine ; buffaloes ; Buffaloes - genetics ; cattle ; Cattle - genetics ; Cell Nucleus - physiology ; Cloning, Organism ; copy number ; Cytoplasm ; DNA, Intergenic ; DNA, Mitochondrial - analysis ; Embryo, Mammalian - chemistry ; Embryo, Mammalian - cytology ; Embryology ; Embryonic Development ; Embryos ; Endangered species ; Fibroblasts ; Fibroblasts - physiology ; intergeneric somatic cell nuclear transfer ; Livestock ; Mitochondrial DNA ; nuclear transfer ; Nuclear Transfer Techniques ; nuclear-cytoplasmic interactions ; Nuclei ; Oocytes ; Progeny ; somatic cell nuclear transfer ; somatic cells ; swamp buffalo ; Swamps</subject><ispartof>Animal science journal, 2011-04, Vol.82 (2), p.236-243</ispartof><rights>2011 The Authors. Journal compilation © 2011 Japanese Society of Animal Science</rights><rights>2011 The Authors. Journal compilation © 2011 Japanese Society of Animal Science.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6577-c5a39fcbad3c6cd2e4aeffb6bc0e72a1e90971fcc37a3df0499ccc8d202285b23</citedby><cites>FETCH-LOGICAL-c6577-c5a39fcbad3c6cd2e4aeffb6bc0e72a1e90971fcc37a3df0499ccc8d202285b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1740-0929.2010.00827.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1740-0929.2010.00827.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21729201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Srirattana, Kanokwan</creatorcontrib><creatorcontrib>Matsukawa, Kazutsugu</creatorcontrib><creatorcontrib>Akagi, Satoshi</creatorcontrib><creatorcontrib>Tasai, Mariko</creatorcontrib><creatorcontrib>Tagami, Takahiro</creatorcontrib><creatorcontrib>Nirasawa, Keijiro</creatorcontrib><creatorcontrib>Nagai, Takashi</creatorcontrib><creatorcontrib>Kanai, Yukio</creatorcontrib><creatorcontrib>Parnpai, Rangsun</creatorcontrib><creatorcontrib>Takeda, Kumiko</creatorcontrib><title>Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm</title><title>Animal science journal</title><addtitle>Anim Sci J</addtitle><description>Although interspecies/intergeneric somatic cell nuclear transfer (iSCNT) has been proposed as a tool to produce offspring of endangered species, conflict between donor nucleus and recipient cytoplasm in iSCNT embryos has been identified as an impediment to implementation for agricultural production. To investigate the nuclear-mitochondrial interactions on the developmental potential of iSCNT embryos, we analyzed the mtDNA copy numbers in iSCNT embryos reconstructed with water buffalo (swamp type) fibroblasts and bovine enucleated oocytes (buffalo iSCNT). As controls, SCNT embryos were derived from bovine fibroblasts (bovine SCNT). Buffalo iSCNT and bovine SCNT embryos showed similar rates of cleavage and development to the 8-cell stage (P > 0.05). However, buffalo iSCNT embryos did not develop beyond the 16-cell stage. Both bovine and buffalo mtDNA content in buffalo iSCNT embryos was stable throughout the nuclear transfer process, and arrested at the 8- to 16-cell stage (P > 0.05). In bovine SCNT embryos that developed to the blastocyst stage, mtDNA copy number was increased (P < 0.05). In conclusion, both the donor cell and recipient cytoplast mtDNAs of buffalo iSCNT embryos were identified and maintained through the iSCNT process until the 8-16-cell stage. In addition, the copy number of mtDNA per embryo was a useful monitor to investigate nuclear-mitochondrial interactions.</description><subject>Animal reproduction</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>blastocyst</subject><subject>blastocysts</subject><subject>bovine</subject><subject>buffaloes</subject><subject>Buffaloes - genetics</subject><subject>cattle</subject><subject>Cattle - genetics</subject><subject>Cell Nucleus - physiology</subject><subject>Cloning, Organism</subject><subject>copy number</subject><subject>Cytoplasm</subject><subject>DNA, Intergenic</subject><subject>DNA, Mitochondrial - analysis</subject><subject>Embryo, Mammalian - chemistry</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryology</subject><subject>Embryonic Development</subject><subject>Embryos</subject><subject>Endangered species</subject><subject>Fibroblasts</subject><subject>Fibroblasts - physiology</subject><subject>intergeneric somatic cell nuclear transfer</subject><subject>Livestock</subject><subject>Mitochondrial DNA</subject><subject>nuclear transfer</subject><subject>Nuclear Transfer Techniques</subject><subject>nuclear-cytoplasmic interactions</subject><subject>Nuclei</subject><subject>Oocytes</subject><subject>Progeny</subject><subject>somatic cell nuclear transfer</subject><subject>somatic cells</subject><subject>swamp buffalo</subject><subject>Swamps</subject><issn>1344-3941</issn><issn>1740-0929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EomXgFcBiwyqD_xLHEpthgBY0KpVKhcTGchy7eEjswU7ozCPw1jikzIINWFfy1b3fOdLVAQBitMT5vdwuMWeoQIKIJUF5ilBN-HJ_D5weF_dzTxkrqGD4BDxKaYsQ5gKVD8EJwZyILDwFP9fBp0H5AQ5R-dS7lFzwMFjYuyHor8G30akOvrlYQedzDSbeGG-i01B3wZsWmr6Jh5BgNHryiqMe8tTG0MN0q_odbEZrVRegdU0MTafSkKDyLWzCD-cNDGGXZ_1j8CBTyTy5-xfg-t3bT-vzYvPx7P16tSl0VXJe6FJRYXWjWqor3RLDlLG2qRqNDCcKG4EEx1ZryhVtLWJCaK3rliBC6rIhdAFezL67GL6PJg0yH61N1ylvwpikQIzxiiH-T7LmJROY5lqA53-R2zBGn8-QdVlTgsuKZqieIR1DStFYuYuuV_EgMZJTrHIrp_TklJ6cYpW_Y5X7LH165z82vWmPwj85ZuDVDNy6zhz-21iurj7kJsuLWe7SYPZHuYrfZMUpL-XnizOJX38Rl5vzS7nJ_LOZtypIdRNdktdX2ZghhATLF9NfPdHMeQ</recordid><startdate>201104</startdate><enddate>201104</enddate><creator>Srirattana, Kanokwan</creator><creator>Matsukawa, Kazutsugu</creator><creator>Akagi, Satoshi</creator><creator>Tasai, Mariko</creator><creator>Tagami, Takahiro</creator><creator>Nirasawa, Keijiro</creator><creator>Nagai, Takashi</creator><creator>Kanai, Yukio</creator><creator>Parnpai, Rangsun</creator><creator>Takeda, Kumiko</creator><general>Blackwell Publishing Asia</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>201104</creationdate><title>Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm</title><author>Srirattana, Kanokwan ; 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To investigate the nuclear-mitochondrial interactions on the developmental potential of iSCNT embryos, we analyzed the mtDNA copy numbers in iSCNT embryos reconstructed with water buffalo (swamp type) fibroblasts and bovine enucleated oocytes (buffalo iSCNT). As controls, SCNT embryos were derived from bovine fibroblasts (bovine SCNT). Buffalo iSCNT and bovine SCNT embryos showed similar rates of cleavage and development to the 8-cell stage (P > 0.05). However, buffalo iSCNT embryos did not develop beyond the 16-cell stage. Both bovine and buffalo mtDNA content in buffalo iSCNT embryos was stable throughout the nuclear transfer process, and arrested at the 8- to 16-cell stage (P > 0.05). In bovine SCNT embryos that developed to the blastocyst stage, mtDNA copy number was increased (P < 0.05). In conclusion, both the donor cell and recipient cytoplast mtDNAs of buffalo iSCNT embryos were identified and maintained through the iSCNT process until the 8-16-cell stage. 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| ispartof | Animal science journal, 2011-04, Vol.82 (2), p.236-243 |
| issn | 1344-3941 1740-0929 |
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| subjects | Animal reproduction Animal sciences Animals blastocyst blastocysts bovine buffaloes Buffaloes - genetics cattle Cattle - genetics Cell Nucleus - physiology Cloning, Organism copy number Cytoplasm DNA, Intergenic DNA, Mitochondrial - analysis Embryo, Mammalian - chemistry Embryo, Mammalian - cytology Embryology Embryonic Development Embryos Endangered species Fibroblasts Fibroblasts - physiology intergeneric somatic cell nuclear transfer Livestock Mitochondrial DNA nuclear transfer Nuclear Transfer Techniques nuclear-cytoplasmic interactions Nuclei Oocytes Progeny somatic cell nuclear transfer somatic cells swamp buffalo Swamps |
| title | Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm |
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