Mechanism and evolution of DNA primases
DNA primase synthesizes short RNA primers that replicative polymerases further elongate in order to initiate the synthesis of all new DNA strands. Thus, primase owes its existence to the inability of DNA polymerases to initiate DNA synthesis starting with 2 dNTPs. Here, we discuss the evolutionary r...
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| Veröffentlicht in: | Biochimica et biophysica acta 2010-05, Vol.1804 (5), p.1180-1189 |
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| container_title | Biochimica et biophysica acta |
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| creator | Kuchta, Robert D. Stengel, Gudrun |
| description | DNA primase synthesizes short RNA primers that replicative polymerases further elongate in order to initiate the synthesis of all new DNA strands. Thus, primase owes its existence to the inability of DNA polymerases to initiate DNA synthesis starting with 2 dNTPs. Here, we discuss the evolutionary relationships between the different families of primases (viral, eubacterial, archael, and eukaryotic) and the catalytic mechanisms of these enzymes. This includes how they choose an initiation site, elongate the growing primer, and then only synthesize primers of defined length via an inherent ability to count. Finally, the low fidelity of primases along with the development of primase inhibitors is described. |
| doi_str_mv | 10.1016/j.bbapap.2009.06.011 |
| format | Article |
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Thus, primase owes its existence to the inability of DNA polymerases to initiate DNA synthesis starting with 2 dNTPs. Here, we discuss the evolutionary relationships between the different families of primases (viral, eubacterial, archael, and eukaryotic) and the catalytic mechanisms of these enzymes. This includes how they choose an initiation site, elongate the growing primer, and then only synthesize primers of defined length via an inherent ability to count. 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Finally, the low fidelity of primases along with the development of primase inhibitors is described.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Counting</subject><subject>DNA Primase - genetics</subject><subject>DNA Primers - genetics</subject><subject>DNA Primers - metabolism</subject><subject>dNTP</subject><subject>Evolution, Molecular</subject><subject>Fidelity</subject><subject>Humans</subject><subject>Inhibition</subject><subject>Initiation</subject><subject>Mechanism</subject><subject>Misincorporation</subject><subject>Molecular Sequence Data</subject><subject>NTP</subject><subject>Polymerase</subject><subject>Primase</subject><subject>Sequence Homology, Amino Acid</subject><issn>1570-9639</issn><issn>0006-3002</issn><issn>1878-1454</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1P3DAQtSqqAlv-QVXlxilhHI_t-FIJ8dVKUC70bDnOBLzKxkucXYl_T5ZdAb3AaUaa997MvMfYDw4FB65O5kVdu6VbFiWAKUAVwPkXdsArXeUcJe5NvdSQGyXMPjtMaQ5QgtbyG9vnRiIYhAN2fEP-wfUhLTLXNxmtY7caQ-yz2Gbnf0-z5RAWLlH6zr62rkt0tKsz9u_y4u7sd359e_Xn7PQ692hwzJVrXCOh0ejQAbWSnNGoXQOVUQgoSvQEhpcCZelF1Rpde2qxrpTmCCBm7NdWd7mqF9R46sfBdfbljOHJRhfs_5M-PNj7uLZlhaoUG4HjncAQH1eURrsIyVPXuZ7iKlkDiFqqsvoUqYWQgkvFJyRukX6IKQ3Uvt7DwW7CsHO7DcNuwrCg7BTGRPv5_pc30s79t2dpcnQdaLDJB-o9NWEgP9omho83PAOlbJtm</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Kuchta, Robert D.</creator><creator>Stengel, Gudrun</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>5PM</scope></search><sort><creationdate>20100501</creationdate><title>Mechanism and evolution of DNA primases</title><author>Kuchta, Robert D. ; Stengel, Gudrun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-6adad50d74a4a0ef5ea9747ad0896404324ce09123452c38f97bcef4b86714003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Counting</topic><topic>DNA Primase - genetics</topic><topic>DNA Primers - genetics</topic><topic>DNA Primers - metabolism</topic><topic>dNTP</topic><topic>Evolution, Molecular</topic><topic>Fidelity</topic><topic>Humans</topic><topic>Inhibition</topic><topic>Initiation</topic><topic>Mechanism</topic><topic>Misincorporation</topic><topic>Molecular Sequence Data</topic><topic>NTP</topic><topic>Polymerase</topic><topic>Primase</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuchta, Robert D.</creatorcontrib><creatorcontrib>Stengel, Gudrun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochimica et biophysica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuchta, Robert D.</au><au>Stengel, Gudrun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism and evolution of DNA primases</atitle><jtitle>Biochimica et biophysica acta</jtitle><addtitle>Biochim Biophys Acta</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>1804</volume><issue>5</issue><spage>1180</spage><epage>1189</epage><pages>1180-1189</pages><issn>1570-9639</issn><issn>0006-3002</issn><eissn>1878-1454</eissn><abstract>DNA primase synthesizes short RNA primers that replicative polymerases further elongate in order to initiate the synthesis of all new DNA strands. 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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Counting DNA Primase - genetics DNA Primers - genetics DNA Primers - metabolism dNTP Evolution, Molecular Fidelity Humans Inhibition Initiation Mechanism Misincorporation Molecular Sequence Data NTP Polymerase Primase Sequence Homology, Amino Acid |
| title | Mechanism and evolution of DNA primases |
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