Placental Glutathione-S-Transferase-Pi mRNA Is Abundantly Expressed in Human Skin
Four overlapping cDNA clones were isolated from a lambda gt11 human placenta cDNA library using purified human IgG antibody, from a patient with bullous pemphigoid. The sequence was homologous to human placenta glutathione-S- transferase-pi (GST-pi). Using the placenta clone, epidermal cDNA clones w...
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| Veröffentlicht in: | Journal of investigative dermatology 1990-08, Vol.95 (2), p.119-126 |
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| container_title | Journal of investigative dermatology |
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| creator | Konohana, Akira Konohana, Izumi Schroeder, Wanda T O'Brien, Wanda R Amagai, Masayuke Greer, Jeannette Shimizu, Noboyoshi Gammon, W Ray Siciliano, Michael J Duvic, Madeleine |
| description | Four overlapping cDNA clones were isolated from a lambda gt11 human placenta cDNA library using purified human IgG antibody, from a patient with bullous pemphigoid. The sequence was homologous to human placenta glutathione-S- transferase-pi (GST-pi). Using the placenta clone, epidermal cDNA clones were isolated from a human keratinocyte library. Expression of GST-pi mRNA in human skin, cultured keratinocytes and fibroblasts, and disorders of squamous hyperplasia was demonstrated by Northern blotting and in situ hybridization. Human epidermal and placental cDNA clones hybridized to the same genomic DNA fragments. Hybridization of placental cDNA to interspecific somatic cell hybrids showed retention of chromosome 11, confirming the assignment of GST 3 to the long arm of chromosome 11 by molecular means. Anti-GST-pi antibody did not give a basement membrane zone pattern, although some normal and BP sera contained antibodies to GST-pi. Human skin expresses glutathione-S-transferase-pi, which belongs to an enzyme family important for detoxification and carcinogenesis. |
| doi_str_mv | 10.1111/1523-1747.ep12477830 |
| format | Article |
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The sequence was homologous to human placenta glutathione-S- transferase-pi (GST-pi). Using the placenta clone, epidermal cDNA clones were isolated from a human keratinocyte library. Expression of GST-pi mRNA in human skin, cultured keratinocytes and fibroblasts, and disorders of squamous hyperplasia was demonstrated by Northern blotting and in situ hybridization. Human epidermal and placental cDNA clones hybridized to the same genomic DNA fragments. Hybridization of placental cDNA to interspecific somatic cell hybrids showed retention of chromosome 11, confirming the assignment of GST 3 to the long arm of chromosome 11 by molecular means. Anti-GST-pi antibody did not give a basement membrane zone pattern, although some normal and BP sera contained antibodies to GST-pi. 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The sequence was homologous to human placenta glutathione-S- transferase-pi (GST-pi). Using the placenta clone, epidermal cDNA clones were isolated from a human keratinocyte library. Expression of GST-pi mRNA in human skin, cultured keratinocytes and fibroblasts, and disorders of squamous hyperplasia was demonstrated by Northern blotting and in situ hybridization. Human epidermal and placental cDNA clones hybridized to the same genomic DNA fragments. Hybridization of placental cDNA to interspecific somatic cell hybrids showed retention of chromosome 11, confirming the assignment of GST 3 to the long arm of chromosome 11 by molecular means. Anti-GST-pi antibody did not give a basement membrane zone pattern, although some normal and BP sera contained antibodies to GST-pi. Human skin expresses glutathione-S-transferase-pi, which belongs to an enzyme family important for detoxification and carcinogenesis.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Squamous Cell - enzymology</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Library</subject><subject>Glutathione Transferase - genetics</subject><subject>Humans</subject><subject>Isoenzymes - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Placenta - enzymology</subject><subject>Pregnancy</subject><subject>RNA, Messenger - genetics</subject><subject>Skin - enzymology</subject><subject>Skin Neoplasms - enzymology</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1rFDEUhoModdv6DxTmQvBqaj4nyY2wlH5B0Wpb8C5kkxOMzmTWZKa0_94su-zemVwE8rznnORB6D3BZ6Suz0RQ1hLJ5RmsCeVSKoZfocX--jVaYExpSzH9-RYdl_IbY9JxoY7QEWUKC8kW6Ptdbx2kyfbNVT9PdvoVxwTtffuQbSoBsi3Q3sVm-PF12dyUZrmak7dp6l-ai-d1hlLANzE11_NgU3P_J6ZT9CbYvsC73XmCHi8vHs6v29tvVzfny9vWMSGm1lrlufKMCgEd0WqlGdPa6g7CStCgLSZUac1l8EorTroAGILvOgsOexnYCfq07bvO498ZymSGWBz0vU0wzsVozLmkGKua5Nuky2MpGYJZ5zjY_GIINhuVZuPMbJyZg8pa9mE3YF4N4PdFO3eVf9xxW5ztQxXmYtnHBOWk7kObZKc5w4F39YNSVv5ly6HKeoqQTXERkgMfM7jJ-DH-_53_AIS7mLY</recordid><startdate>199008</startdate><enddate>199008</enddate><creator>Konohana, Akira</creator><creator>Konohana, Izumi</creator><creator>Schroeder, Wanda T</creator><creator>O'Brien, Wanda R</creator><creator>Amagai, Masayuke</creator><creator>Greer, Jeannette</creator><creator>Shimizu, Noboyoshi</creator><creator>Gammon, W Ray</creator><creator>Siciliano, Michael J</creator><creator>Duvic, Madeleine</creator><general>Elsevier Inc</general><general>Nature Publishing</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>199008</creationdate><title>Placental Glutathione-S-Transferase-Pi mRNA Is Abundantly Expressed in Human Skin</title><author>Konohana, Akira ; Konohana, Izumi ; Schroeder, Wanda T ; O'Brien, Wanda R ; Amagai, Masayuke ; Greer, Jeannette ; Shimizu, Noboyoshi ; Gammon, W Ray ; Siciliano, Michael J ; Duvic, Madeleine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-aa8d48d3255e6198b93399a96efb52f9a01289947fd898416fe0efd66aec0d7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Squamous Cell - enzymology</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Library</topic><topic>Glutathione Transferase - genetics</topic><topic>Humans</topic><topic>Isoenzymes - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Placenta - enzymology</topic><topic>Pregnancy</topic><topic>RNA, Messenger - genetics</topic><topic>Skin - enzymology</topic><topic>Skin Neoplasms - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Konohana, Akira</creatorcontrib><creatorcontrib>Konohana, Izumi</creatorcontrib><creatorcontrib>Schroeder, Wanda T</creatorcontrib><creatorcontrib>O'Brien, Wanda R</creatorcontrib><creatorcontrib>Amagai, Masayuke</creatorcontrib><creatorcontrib>Greer, Jeannette</creatorcontrib><creatorcontrib>Shimizu, Noboyoshi</creatorcontrib><creatorcontrib>Gammon, W Ray</creatorcontrib><creatorcontrib>Siciliano, Michael J</creatorcontrib><creatorcontrib>Duvic, Madeleine</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Konohana, Akira</au><au>Konohana, Izumi</au><au>Schroeder, Wanda T</au><au>O'Brien, Wanda R</au><au>Amagai, Masayuke</au><au>Greer, Jeannette</au><au>Shimizu, Noboyoshi</au><au>Gammon, W Ray</au><au>Siciliano, Michael J</au><au>Duvic, Madeleine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Placental Glutathione-S-Transferase-Pi mRNA Is Abundantly Expressed in Human Skin</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1990-08</date><risdate>1990</risdate><volume>95</volume><issue>2</issue><spage>119</spage><epage>126</epage><pages>119-126</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>Four overlapping cDNA clones were isolated from a lambda gt11 human placenta cDNA library using purified human IgG antibody, from a patient with bullous pemphigoid. The sequence was homologous to human placenta glutathione-S- transferase-pi (GST-pi). Using the placenta clone, epidermal cDNA clones were isolated from a human keratinocyte library. Expression of GST-pi mRNA in human skin, cultured keratinocytes and fibroblasts, and disorders of squamous hyperplasia was demonstrated by Northern blotting and in situ hybridization. Human epidermal and placental cDNA clones hybridized to the same genomic DNA fragments. Hybridization of placental cDNA to interspecific somatic cell hybrids showed retention of chromosome 11, confirming the assignment of GST 3 to the long arm of chromosome 11 by molecular means. Anti-GST-pi antibody did not give a basement membrane zone pattern, although some normal and BP sera contained antibodies to GST-pi. 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| ispartof | Journal of investigative dermatology, 1990-08, Vol.95 (2), p.119-126 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Carcinoma, Squamous Cell - enzymology Chromosome Mapping Chromosomes, Human Cloning, Molecular DNA - genetics Female Fundamental and applied biological sciences. Psychology Gene Expression Gene Library Glutathione Transferase - genetics Humans Isoenzymes - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Hybridization Placenta - enzymology Pregnancy RNA, Messenger - genetics Skin - enzymology Skin Neoplasms - enzymology |
| title | Placental Glutathione-S-Transferase-Pi mRNA Is Abundantly Expressed in Human Skin |
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