Small-animal PET of tumor damage induced by photothermal ablation with 64Cu-bis-DOTA-hypericin
The purpose of this study was to investigate the potential application of small-molecular-weight (64)Cu-labeled bis-DOTA-hypericin in the noninvasive assessment of response to photothermal ablation therapy. Bis-DOTA-hypericin was labeled with (64)Cu with high efficiency (>95% without purification...
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Veröffentlicht in: | The Journal of nuclear medicine (1978) 2011-05, Vol.52 (5), p.792-799 |
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creator | Song, Shaoli Xiong, Chiyi Zhou, Min Lu, Wei Huang, Qian Ku, Geng Zhao, Jun Flores, Jr, Leo G Ni, Yicheng Li, Chun |
description | The purpose of this study was to investigate the potential application of small-molecular-weight (64)Cu-labeled bis-DOTA-hypericin in the noninvasive assessment of response to photothermal ablation therapy.
Bis-DOTA-hypericin was labeled with (64)Cu with high efficiency (>95% without purification). Nine mice bearing subcutaneous human mammary BT474 tumors were used. Five mice were injected intratumorally with semiconductor CuS nanoparticles, followed by near-infrared laser irradiation 24 h later (12 W/cm(2) for 3 min), and 4 mice were not treated (control group). All mice were intravenously injected with (64)Cu-bis-DOTA-hypericin (24 h after laser treatment in treated mice). Small-animal PET images were acquired at 2, 6, and 24 h after radiotracer injection. All mice were killed immediately after the imaging session for biodistribution and histology study. In vitro cell uptake and surface plasmon resonance studies were performed to validate the small-animal PET results.
(64)Cu-bis-DOTA-hypericin uptake was significantly higher in the treatment group than in the control group. The percentage injected dose per gram of tissue in the treated and control groups was 1.72 ± 0.43 and 0.76 ± 0.19, respectively (P = 0.017), at 24 h after injection. Autoradiography and histology results were consistent with selective uptake of the radiotracer in the necrotic zone of the tumor induced by photothermal ablation therapy. In vitro results showed that treated BT474 cells had a higher uptake of (64)Cu-bis-DOTA-hypericin than nontreated cells. Surface plasmon resonance study showed that bis-DOTA-hypericin had higher binding affinity to phosphatidylserine and phosphatidylethanolamine than to phosphatidylcholine.
(64)Cu-bis-DOTA-hypericin has a potential to image thermal therapy-induced tumor cell damage. The affinity of (64)Cu-bis-DOTA-hypericin for injured tissues may be attributed to the breakdown of the cell membrane and exposure of phosphatidylserine or phosphatidylethanolamine to the radiotracer, which binds selectively to these phospholipids. |
doi_str_mv | 10.2967/jnumed.110.086116 |
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Bis-DOTA-hypericin was labeled with (64)Cu with high efficiency (>95% without purification). Nine mice bearing subcutaneous human mammary BT474 tumors were used. Five mice were injected intratumorally with semiconductor CuS nanoparticles, followed by near-infrared laser irradiation 24 h later (12 W/cm(2) for 3 min), and 4 mice were not treated (control group). All mice were intravenously injected with (64)Cu-bis-DOTA-hypericin (24 h after laser treatment in treated mice). Small-animal PET images were acquired at 2, 6, and 24 h after radiotracer injection. All mice were killed immediately after the imaging session for biodistribution and histology study. In vitro cell uptake and surface plasmon resonance studies were performed to validate the small-animal PET results.
(64)Cu-bis-DOTA-hypericin uptake was significantly higher in the treatment group than in the control group. The percentage injected dose per gram of tissue in the treated and control groups was 1.72 ± 0.43 and 0.76 ± 0.19, respectively (P = 0.017), at 24 h after injection. Autoradiography and histology results were consistent with selective uptake of the radiotracer in the necrotic zone of the tumor induced by photothermal ablation therapy. In vitro results showed that treated BT474 cells had a higher uptake of (64)Cu-bis-DOTA-hypericin than nontreated cells. Surface plasmon resonance study showed that bis-DOTA-hypericin had higher binding affinity to phosphatidylserine and phosphatidylethanolamine than to phosphatidylcholine.
(64)Cu-bis-DOTA-hypericin has a potential to image thermal therapy-induced tumor cell damage. The affinity of (64)Cu-bis-DOTA-hypericin for injured tissues may be attributed to the breakdown of the cell membrane and exposure of phosphatidylserine or phosphatidylethanolamine to the radiotracer, which binds selectively to these phospholipids.</description><identifier>ISSN: 0161-5505</identifier><identifier>EISSN: 1535-5667</identifier><identifier>DOI: 10.2967/jnumed.110.086116</identifier><identifier>PMID: 21498539</identifier><language>eng</language><publisher>United States</publisher><subject>Ablation Techniques ; Animals ; Biological Transport - drug effects ; Breast Neoplasms - diagnostic imaging ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Breast Neoplasms - surgery ; Cell Line, Tumor ; Cell Transformation, Neoplastic ; Female ; Humans ; Mice ; Mice, Nude ; Nanoparticles - chemistry ; Organometallic Compounds - chemical synthesis ; Organometallic Compounds - metabolism ; Organometallic Compounds - pharmacokinetics ; Peptides - metabolism ; Perylene - analogs & derivatives ; Perylene - chemical synthesis ; Perylene - metabolism ; Perylene - pharmacokinetics ; Phosphatidylserines - metabolism ; Positron-Emission Tomography - methods ; Sulfides - chemistry ; Sulfides - pharmacology ; Surface Plasmon Resonance ; Treatment Outcome</subject><ispartof>The Journal of nuclear medicine (1978), 2011-05, Vol.52 (5), p.792-799</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21498539$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Shaoli</creatorcontrib><creatorcontrib>Xiong, Chiyi</creatorcontrib><creatorcontrib>Zhou, Min</creatorcontrib><creatorcontrib>Lu, Wei</creatorcontrib><creatorcontrib>Huang, Qian</creatorcontrib><creatorcontrib>Ku, Geng</creatorcontrib><creatorcontrib>Zhao, Jun</creatorcontrib><creatorcontrib>Flores, Jr, Leo G</creatorcontrib><creatorcontrib>Ni, Yicheng</creatorcontrib><creatorcontrib>Li, Chun</creatorcontrib><title>Small-animal PET of tumor damage induced by photothermal ablation with 64Cu-bis-DOTA-hypericin</title><title>The Journal of nuclear medicine (1978)</title><addtitle>J Nucl Med</addtitle><description>The purpose of this study was to investigate the potential application of small-molecular-weight (64)Cu-labeled bis-DOTA-hypericin in the noninvasive assessment of response to photothermal ablation therapy.
Bis-DOTA-hypericin was labeled with (64)Cu with high efficiency (>95% without purification). Nine mice bearing subcutaneous human mammary BT474 tumors were used. Five mice were injected intratumorally with semiconductor CuS nanoparticles, followed by near-infrared laser irradiation 24 h later (12 W/cm(2) for 3 min), and 4 mice were not treated (control group). All mice were intravenously injected with (64)Cu-bis-DOTA-hypericin (24 h after laser treatment in treated mice). Small-animal PET images were acquired at 2, 6, and 24 h after radiotracer injection. All mice were killed immediately after the imaging session for biodistribution and histology study. In vitro cell uptake and surface plasmon resonance studies were performed to validate the small-animal PET results.
(64)Cu-bis-DOTA-hypericin uptake was significantly higher in the treatment group than in the control group. The percentage injected dose per gram of tissue in the treated and control groups was 1.72 ± 0.43 and 0.76 ± 0.19, respectively (P = 0.017), at 24 h after injection. Autoradiography and histology results were consistent with selective uptake of the radiotracer in the necrotic zone of the tumor induced by photothermal ablation therapy. In vitro results showed that treated BT474 cells had a higher uptake of (64)Cu-bis-DOTA-hypericin than nontreated cells. Surface plasmon resonance study showed that bis-DOTA-hypericin had higher binding affinity to phosphatidylserine and phosphatidylethanolamine than to phosphatidylcholine.
(64)Cu-bis-DOTA-hypericin has a potential to image thermal therapy-induced tumor cell damage. The affinity of (64)Cu-bis-DOTA-hypericin for injured tissues may be attributed to the breakdown of the cell membrane and exposure of phosphatidylserine or phosphatidylethanolamine to the radiotracer, which binds selectively to these phospholipids.</description><subject>Ablation Techniques</subject><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Breast Neoplasms - diagnostic imaging</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Breast Neoplasms - surgery</subject><subject>Cell Line, Tumor</subject><subject>Cell Transformation, Neoplastic</subject><subject>Female</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Nanoparticles - chemistry</subject><subject>Organometallic Compounds - chemical synthesis</subject><subject>Organometallic Compounds - metabolism</subject><subject>Organometallic Compounds - pharmacokinetics</subject><subject>Peptides - metabolism</subject><subject>Perylene - analogs & derivatives</subject><subject>Perylene - chemical synthesis</subject><subject>Perylene - metabolism</subject><subject>Perylene - pharmacokinetics</subject><subject>Phosphatidylserines - metabolism</subject><subject>Positron-Emission Tomography - methods</subject><subject>Sulfides - chemistry</subject><subject>Sulfides - pharmacology</subject><subject>Surface Plasmon Resonance</subject><subject>Treatment Outcome</subject><issn>0161-5505</issn><issn>1535-5667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKAzEYRoMotlYfwI1k5yr1z2RyW5ZaL1CoYN06JJnEpszNuSB9e0esa1eHDw7f4iB0TWGeaCHv9tVQ-nxOxw1KUCpO0JRyxgkXQp6iKVBBCefAJ-ii6_YAIJRS52iS0FQrzvQUvb-WpiiIqeJI_LLa4jrgfijrFuemNB8exyofnM-xPeBmV_d1v_Ptj2tsYfpYV_gr9jss0uVAbOzI_Wa7ILtD49voYnWJzoIpOn915Ay9Pay2yyey3jw-Lxdr0lAGggTnQFinfG6okT4oFaxKgPo8OG5pMIYJyYJONDiQwjBQlnLNFPXAAFI2Q7e_v01bfw6-67Myds4Xhal8PXSZhjSVoBn911QilYrLNBnNm6M52DFz1rRjo_aQ_cVj32sjcYo</recordid><startdate>201105</startdate><enddate>201105</enddate><creator>Song, Shaoli</creator><creator>Xiong, Chiyi</creator><creator>Zhou, Min</creator><creator>Lu, Wei</creator><creator>Huang, Qian</creator><creator>Ku, Geng</creator><creator>Zhao, Jun</creator><creator>Flores, Jr, Leo G</creator><creator>Ni, Yicheng</creator><creator>Li, Chun</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201105</creationdate><title>Small-animal PET of tumor damage induced by photothermal ablation with 64Cu-bis-DOTA-hypericin</title><author>Song, Shaoli ; Xiong, Chiyi ; Zhou, Min ; Lu, Wei ; Huang, Qian ; Ku, Geng ; Zhao, Jun ; Flores, Jr, Leo G ; Ni, Yicheng ; Li, Chun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1306-fcc06bc8eda1a7ef88fb8201edfc5b1faa3673f9290c076a308b159381e030043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Ablation Techniques</topic><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Breast Neoplasms - diagnostic imaging</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Breast Neoplasms - surgery</topic><topic>Cell Line, Tumor</topic><topic>Cell Transformation, Neoplastic</topic><topic>Female</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Nanoparticles - chemistry</topic><topic>Organometallic Compounds - chemical synthesis</topic><topic>Organometallic Compounds - metabolism</topic><topic>Organometallic Compounds - pharmacokinetics</topic><topic>Peptides - metabolism</topic><topic>Perylene - analogs & derivatives</topic><topic>Perylene - chemical synthesis</topic><topic>Perylene - metabolism</topic><topic>Perylene - pharmacokinetics</topic><topic>Phosphatidylserines - metabolism</topic><topic>Positron-Emission Tomography - methods</topic><topic>Sulfides - chemistry</topic><topic>Sulfides - pharmacology</topic><topic>Surface Plasmon Resonance</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Shaoli</creatorcontrib><creatorcontrib>Xiong, Chiyi</creatorcontrib><creatorcontrib>Zhou, Min</creatorcontrib><creatorcontrib>Lu, Wei</creatorcontrib><creatorcontrib>Huang, Qian</creatorcontrib><creatorcontrib>Ku, Geng</creatorcontrib><creatorcontrib>Zhao, Jun</creatorcontrib><creatorcontrib>Flores, Jr, Leo G</creatorcontrib><creatorcontrib>Ni, Yicheng</creatorcontrib><creatorcontrib>Li, Chun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>The Journal of nuclear medicine (1978)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Shaoli</au><au>Xiong, Chiyi</au><au>Zhou, Min</au><au>Lu, Wei</au><au>Huang, Qian</au><au>Ku, Geng</au><au>Zhao, Jun</au><au>Flores, Jr, Leo G</au><au>Ni, Yicheng</au><au>Li, Chun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Small-animal PET of tumor damage induced by photothermal ablation with 64Cu-bis-DOTA-hypericin</atitle><jtitle>The Journal of nuclear medicine (1978)</jtitle><addtitle>J Nucl Med</addtitle><date>2011-05</date><risdate>2011</risdate><volume>52</volume><issue>5</issue><spage>792</spage><epage>799</epage><pages>792-799</pages><issn>0161-5505</issn><eissn>1535-5667</eissn><abstract>The purpose of this study was to investigate the potential application of small-molecular-weight (64)Cu-labeled bis-DOTA-hypericin in the noninvasive assessment of response to photothermal ablation therapy.
Bis-DOTA-hypericin was labeled with (64)Cu with high efficiency (>95% without purification). Nine mice bearing subcutaneous human mammary BT474 tumors were used. Five mice were injected intratumorally with semiconductor CuS nanoparticles, followed by near-infrared laser irradiation 24 h later (12 W/cm(2) for 3 min), and 4 mice were not treated (control group). All mice were intravenously injected with (64)Cu-bis-DOTA-hypericin (24 h after laser treatment in treated mice). Small-animal PET images were acquired at 2, 6, and 24 h after radiotracer injection. All mice were killed immediately after the imaging session for biodistribution and histology study. In vitro cell uptake and surface plasmon resonance studies were performed to validate the small-animal PET results.
(64)Cu-bis-DOTA-hypericin uptake was significantly higher in the treatment group than in the control group. The percentage injected dose per gram of tissue in the treated and control groups was 1.72 ± 0.43 and 0.76 ± 0.19, respectively (P = 0.017), at 24 h after injection. Autoradiography and histology results were consistent with selective uptake of the radiotracer in the necrotic zone of the tumor induced by photothermal ablation therapy. In vitro results showed that treated BT474 cells had a higher uptake of (64)Cu-bis-DOTA-hypericin than nontreated cells. Surface plasmon resonance study showed that bis-DOTA-hypericin had higher binding affinity to phosphatidylserine and phosphatidylethanolamine than to phosphatidylcholine.
(64)Cu-bis-DOTA-hypericin has a potential to image thermal therapy-induced tumor cell damage. The affinity of (64)Cu-bis-DOTA-hypericin for injured tissues may be attributed to the breakdown of the cell membrane and exposure of phosphatidylserine or phosphatidylethanolamine to the radiotracer, which binds selectively to these phospholipids.</abstract><cop>United States</cop><pmid>21498539</pmid><doi>10.2967/jnumed.110.086116</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Ablation Techniques Animals Biological Transport - drug effects Breast Neoplasms - diagnostic imaging Breast Neoplasms - metabolism Breast Neoplasms - pathology Breast Neoplasms - surgery Cell Line, Tumor Cell Transformation, Neoplastic Female Humans Mice Mice, Nude Nanoparticles - chemistry Organometallic Compounds - chemical synthesis Organometallic Compounds - metabolism Organometallic Compounds - pharmacokinetics Peptides - metabolism Perylene - analogs & derivatives Perylene - chemical synthesis Perylene - metabolism Perylene - pharmacokinetics Phosphatidylserines - metabolism Positron-Emission Tomography - methods Sulfides - chemistry Sulfides - pharmacology Surface Plasmon Resonance Treatment Outcome |
title | Small-animal PET of tumor damage induced by photothermal ablation with 64Cu-bis-DOTA-hypericin |
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