Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation
Maf1 protein is a global negative regulator of RNA polymerase (Pol) III transcription conserved from yeast to man. We report that phosphorylation of Maf1 by casein kinase II (CK2), a highly evolutionarily conserved eukaryotic kinase, is required for efficient Pol III transcription. Both recombinant...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2011-03, Vol.108 (12), p.4926-4931 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Graczyk, Damian DÄbski, Janusz MuszyÅska, Grażyna Bretner, Maria Lefebvre, Olivier Boguta, Magdalena |
| description | Maf1 protein is a global negative regulator of RNA polymerase (Pol) III transcription conserved from yeast to man. We report that phosphorylation of Maf1 by casein kinase II (CK2), a highly evolutionarily conserved eukaryotic kinase, is required for efficient Pol III transcription. Both recombinant human and yeast CK2 were able to phosphorylate purified human or yeast Maf1, indicating that Maf1 can be a direct substrate of CK2. Upon transfer of Saccharomyces cerevisiae from repressive to favorable growth conditions, CK2 activity is required for the release of Maf1 from Pol III bound to a tRNA gene and for subsequent activation of tRNA transcription. In a yeast strain lacking Maf1, CK2 inhibition showed no effect on tRNA synthesis, confirming that CK2 activates Pol III via Maf1. Additionally, CK2 was found to associate with tRNA genes, and this association is enhanced in absence of Maf1, especially under repressive conditions. These results corroborate the previously reported TFIIIB-CK2 interaction and indicate an important role of CK2-mediated Maf1 phosphorylation in triggering Pol III activation. |
| doi_str_mv | 10.1073/pnas.1010010108 |
| format | Article |
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We report that phosphorylation of Maf1 by casein kinase II (CK2), a highly evolutionarily conserved eukaryotic kinase, is required for efficient Pol III transcription. Both recombinant human and yeast CK2 were able to phosphorylate purified human or yeast Maf1, indicating that Maf1 can be a direct substrate of CK2. Upon transfer of Saccharomyces cerevisiae from repressive to favorable growth conditions, CK2 activity is required for the release of Maf1 from Pol III bound to a tRNA gene and for subsequent activation of tRNA transcription. In a yeast strain lacking Maf1, CK2 inhibition showed no effect on tRNA synthesis, confirming that CK2 activates Pol III via Maf1. Additionally, CK2 was found to associate with tRNA genes, and this association is enhanced in absence of Maf1, especially under repressive conditions. These results corroborate the previously reported TFIIIB-CK2 interaction and indicate an important role of CK2-mediated Maf1 phosphorylation in triggering Pol III activation.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1010010108</identifier><identifier>PMID: 21383183</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Biological Sciences ; Casein ; Casein kinase II ; Casein Kinase II - genetics ; Casein Kinase II - metabolism ; Cell cycle ; DNA-directed RNA polymerase ; Enzyme Activation ; Evolution ; Gene expression regulation ; Genes ; Growth conditions ; Humans ; MAF1 protein ; non-specific serine/threonine protein kinase ; Phosphorylation ; Proteins ; Repression ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Repressors ; RNA ; RNA polymerase ; RNA Polymerase III - genetics ; RNA Polymerase III - metabolism ; RNA, Transfer - biosynthesis ; RNA, Transfer - genetics ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Transcription ; Transcription Factor TFIIIB - genetics ; Transcription Factor TFIIIB - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic - physiology ; Transcriptional regulatory elements ; Transfer RNA ; tRNA ; Yeast ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2011-03, Vol.108 (12), p.4926-4931</ispartof><rights>copyright © 1993-2008 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Mar 22, 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-3824de8ca0050473bcdf17554203e3ed01114683bdfc9072dfeebb3f09d00e573</citedby><cites>FETCH-LOGICAL-c521t-3824de8ca0050473bcdf17554203e3ed01114683bdfc9072dfeebb3f09d00e573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/108/12.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41125246$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41125246$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21383183$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Graczyk, Damian</creatorcontrib><creatorcontrib>DÄbski, Janusz</creatorcontrib><creatorcontrib>MuszyÅska, Grażyna</creatorcontrib><creatorcontrib>Bretner, Maria</creatorcontrib><creatorcontrib>Lefebvre, Olivier</creatorcontrib><creatorcontrib>Boguta, Magdalena</creatorcontrib><title>Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Maf1 protein is a global negative regulator of RNA polymerase (Pol) III transcription conserved from yeast to man. We report that phosphorylation of Maf1 by casein kinase II (CK2), a highly evolutionarily conserved eukaryotic kinase, is required for efficient Pol III transcription. Both recombinant human and yeast CK2 were able to phosphorylate purified human or yeast Maf1, indicating that Maf1 can be a direct substrate of CK2. Upon transfer of Saccharomyces cerevisiae from repressive to favorable growth conditions, CK2 activity is required for the release of Maf1 from Pol III bound to a tRNA gene and for subsequent activation of tRNA transcription. In a yeast strain lacking Maf1, CK2 inhibition showed no effect on tRNA synthesis, confirming that CK2 activates Pol III via Maf1. Additionally, CK2 was found to associate with tRNA genes, and this association is enhanced in absence of Maf1, especially under repressive conditions. These results corroborate the previously reported TFIIIB-CK2 interaction and indicate an important role of CK2-mediated Maf1 phosphorylation in triggering Pol III activation.</description><subject>Biological Sciences</subject><subject>Casein</subject><subject>Casein kinase II</subject><subject>Casein Kinase II - genetics</subject><subject>Casein Kinase II - metabolism</subject><subject>Cell cycle</subject><subject>DNA-directed RNA polymerase</subject><subject>Enzyme Activation</subject><subject>Evolution</subject><subject>Gene expression regulation</subject><subject>Genes</subject><subject>Growth conditions</subject><subject>Humans</subject><subject>MAF1 protein</subject><subject>non-specific serine/threonine protein kinase</subject><subject>Phosphorylation</subject><subject>Proteins</subject><subject>Repression</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Repressors</subject><subject>RNA</subject><subject>RNA polymerase</subject><subject>RNA Polymerase III - genetics</subject><subject>RNA Polymerase III - metabolism</subject><subject>RNA, Transfer - biosynthesis</subject><subject>RNA, Transfer - genetics</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Transcription</subject><subject>Transcription Factor TFIIIB - genetics</subject><subject>Transcription Factor TFIIIB - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic - physiology</subject><subject>Transcriptional regulatory elements</subject><subject>Transfer RNA</subject><subject>tRNA</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P0zAQhi0EYsvCmRNgceEUdvyRxL4grSoWKi0gAXu2nGTSdUnjrJ2u1H-_Tlta4MLBH5KfefSOh5CXDN4zKMXF0NuYbgxg2tQjMmOgWVZIDY_JDICXmZJcnpFnMa4AQOcKnpIzzoQSTIkZWc1tRNfTXy6ZkC4W2RobZ0ds6HDrY1ph29nR-Z76li6xx2A7GnAIGKMP9IttGR2DWy4xRPr96yUdfLddJ2pnW1Bbj-5-J3hOnrS2i_jicJ6Tm6uPP-efs-tvnxbzy-uszjkbM6G4bFDVFiAHWYqqblpW5rnkIFBgA4wxWShRNW2toeRNi1hVogXdAGBeinPyYe8dNlVqpsZ-TJnNENzahq3x1pm_X3p3a5b-3ggopJCQBO8OguDvNhhHs3axxq6zPfpNNBpkCqBL_V9S5UqysuCT8-0_5MpvQp_-YQflpcpFgi72UB18jAHbY2gGZpq3meZtTvNOFa__7PXI_x5wAt4cgKnypFOGcSM1LxLxak-s4ujDEZGM8ZzL4mRorTd2GVw0Nz84sCJl0Fyn2A_mM8Qa</recordid><startdate>20110322</startdate><enddate>20110322</enddate><creator>Graczyk, Damian</creator><creator>DÄbski, Janusz</creator><creator>MuszyÅska, Grażyna</creator><creator>Bretner, Maria</creator><creator>Lefebvre, Olivier</creator><creator>Boguta, Magdalena</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110322</creationdate><title>Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation</title><author>Graczyk, Damian ; DÄbski, Janusz ; MuszyÅska, Grażyna ; Bretner, Maria ; Lefebvre, Olivier ; Boguta, Magdalena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-3824de8ca0050473bcdf17554203e3ed01114683bdfc9072dfeebb3f09d00e573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biological Sciences</topic><topic>Casein</topic><topic>Casein kinase II</topic><topic>Casein Kinase II - genetics</topic><topic>Casein Kinase II - metabolism</topic><topic>Cell cycle</topic><topic>DNA-directed RNA polymerase</topic><topic>Enzyme Activation</topic><topic>Evolution</topic><topic>Gene expression regulation</topic><topic>Genes</topic><topic>Growth conditions</topic><topic>Humans</topic><topic>MAF1 protein</topic><topic>non-specific serine/threonine protein kinase</topic><topic>Phosphorylation</topic><topic>Proteins</topic><topic>Repression</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Repressors</topic><topic>RNA</topic><topic>RNA polymerase</topic><topic>RNA Polymerase III - genetics</topic><topic>RNA Polymerase III - metabolism</topic><topic>RNA, Transfer - biosynthesis</topic><topic>RNA, Transfer - genetics</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Transcription</topic><topic>Transcription Factor TFIIIB - genetics</topic><topic>Transcription Factor TFIIIB - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic - physiology</topic><topic>Transcriptional regulatory elements</topic><topic>Transfer RNA</topic><topic>tRNA</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Graczyk, Damian</creatorcontrib><creatorcontrib>DÄbski, Janusz</creatorcontrib><creatorcontrib>MuszyÅska, Grażyna</creatorcontrib><creatorcontrib>Bretner, Maria</creatorcontrib><creatorcontrib>Lefebvre, Olivier</creatorcontrib><creatorcontrib>Boguta, Magdalena</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Graczyk, Damian</au><au>DÄbski, Janusz</au><au>MuszyÅska, Grażyna</au><au>Bretner, Maria</au><au>Lefebvre, Olivier</au><au>Boguta, Magdalena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2011-03-22</date><risdate>2011</risdate><volume>108</volume><issue>12</issue><spage>4926</spage><epage>4931</epage><pages>4926-4931</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Maf1 protein is a global negative regulator of RNA polymerase (Pol) III transcription conserved from yeast to man. We report that phosphorylation of Maf1 by casein kinase II (CK2), a highly evolutionarily conserved eukaryotic kinase, is required for efficient Pol III transcription. Both recombinant human and yeast CK2 were able to phosphorylate purified human or yeast Maf1, indicating that Maf1 can be a direct substrate of CK2. Upon transfer of Saccharomyces cerevisiae from repressive to favorable growth conditions, CK2 activity is required for the release of Maf1 from Pol III bound to a tRNA gene and for subsequent activation of tRNA transcription. In a yeast strain lacking Maf1, CK2 inhibition showed no effect on tRNA synthesis, confirming that CK2 activates Pol III via Maf1. Additionally, CK2 was found to associate with tRNA genes, and this association is enhanced in absence of Maf1, especially under repressive conditions. These results corroborate the previously reported TFIIIB-CK2 interaction and indicate an important role of CK2-mediated Maf1 phosphorylation in triggering Pol III activation.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>21383183</pmid><doi>10.1073/pnas.1010010108</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological Sciences Casein Casein kinase II Casein Kinase II - genetics Casein Kinase II - metabolism Cell cycle DNA-directed RNA polymerase Enzyme Activation Evolution Gene expression regulation Genes Growth conditions Humans MAF1 protein non-specific serine/threonine protein kinase Phosphorylation Proteins Repression Repressor Proteins - genetics Repressor Proteins - metabolism Repressors RNA RNA polymerase RNA Polymerase III - genetics RNA Polymerase III - metabolism RNA, Transfer - biosynthesis RNA, Transfer - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Transcription Transcription Factor TFIIIB - genetics Transcription Factor TFIIIB - metabolism Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic - physiology Transcriptional regulatory elements Transfer RNA tRNA Yeast Yeasts |
| title | Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation |
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