Novel application of κ-carrageenan: As a gelling agent in microbiological media to study biodiversity of extreme alkaliphiles
In the present investigation a novel application of κ-carrageenan, as a gelling agent for isolation, purification and identification of extreme alkaliphilic bacteria has been explored. The potassium salt of κ-carrageenan was an appropriate substitute of agar for preparation of solid medium, particul...
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| Veröffentlicht in: | Carbohydrate polymers 2011-05, Vol.85 (2), p.465-468 |
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| container_title | Carbohydrate polymers |
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| creator | Datta, Sumitra Mody, Kalpana Gopalsamy, Gnanasekaran Jha, Bhavanath |
| description | In the present investigation a novel application of κ-carrageenan, as a gelling agent for isolation, purification and identification of extreme alkaliphilic bacteria has been explored. The potassium salt of κ-carrageenan was an appropriate substitute of agar for preparation of solid medium, particularly for isolation of extreme alkaliphiles, as agar de-polymerizes at pH
>
12.5. The purpose of formulating a new, stable, highly alkaline, solid medium was to study the diversity of extreme alkaliphilic bacteria by means of conventional cultivation techniques allied with molecular tools. The medium having pH in the range of 8.0–13.5 was used to isolate extreme alkaliphiles in pure form. |
| doi_str_mv | 10.1016/j.carbpol.2011.02.036 |
| format | Article |
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>
12.5. The purpose of formulating a new, stable, highly alkaline, solid medium was to study the diversity of extreme alkaliphilic bacteria by means of conventional cultivation techniques allied with molecular tools. The medium having pH in the range of 8.0–13.5 was used to isolate extreme alkaliphiles in pure form.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2011.02.036</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Alkaliphiles ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Gelling agent ; Kappaphycus alvarezii ; Microbiological media ; Microbiology ; κ-Carrageenan</subject><ispartof>Carbohydrate polymers, 2011-05, Vol.85 (2), p.465-468</ispartof><rights>2011 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-60273f8352410f315615d45eb04363f810f07c7f148cd5708707ff241e61ecdd3</citedby><cites>FETCH-LOGICAL-c371t-60273f8352410f315615d45eb04363f810f07c7f148cd5708707ff241e61ecdd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carbpol.2011.02.036$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24122929$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Datta, Sumitra</creatorcontrib><creatorcontrib>Mody, Kalpana</creatorcontrib><creatorcontrib>Gopalsamy, Gnanasekaran</creatorcontrib><creatorcontrib>Jha, Bhavanath</creatorcontrib><title>Novel application of κ-carrageenan: As a gelling agent in microbiological media to study biodiversity of extreme alkaliphiles</title><title>Carbohydrate polymers</title><description>In the present investigation a novel application of κ-carrageenan, as a gelling agent for isolation, purification and identification of extreme alkaliphilic bacteria has been explored. The potassium salt of κ-carrageenan was an appropriate substitute of agar for preparation of solid medium, particularly for isolation of extreme alkaliphiles, as agar de-polymerizes at pH
>
12.5. The purpose of formulating a new, stable, highly alkaline, solid medium was to study the diversity of extreme alkaliphilic bacteria by means of conventional cultivation techniques allied with molecular tools. The medium having pH in the range of 8.0–13.5 was used to isolate extreme alkaliphiles in pure form.</description><subject>Alkaliphiles</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gelling agent</subject><subject>Kappaphycus alvarezii</subject><subject>Microbiological media</subject><subject>Microbiology</subject><subject>κ-Carrageenan</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkM2u0zAQhS0EEuXCIyB5g1gl2IljJ2zQ1RV_0hVsYG25zri4OHaw3YpueDAegmdiqlZs8cbSzDkzcz5CnnPWcsblq31rTd6uKbQd47xlXct6-YBs-KimhvdCPCQbxoVoRsnVY_KklD3DJznbkF-f0hECNesavDXVp0iTo39-Nzgymx1ANPE1vS3U0B2E4OOOYjVW6iNdvM1p61NIO_QGusDsDa2JlnqYTxQ7sz9CLr6ezkPhZ82wADXhuwl-_eYDlKfkkTOhwLPrf0O-vnv75e5Dc__5_ce72_vG9orXRrJO9W7sh05w5no-SD7MYoAtE73EBhaZsspxMdp5UGxUTDmHYpAc7Dz3N-TlZe6a048DlKoXXywGMhHSoeiJCSFHOXFUDhclZislg9Nr9ovJJ82ZPuPWe33Frc-4Nes04kbfi-sGUxCGyyZaX_6Z8Zaum7oJdW8uOsC4Rw9ZF-shWmSXwVY9J_-fTX8BhciaOA</recordid><startdate>20110506</startdate><enddate>20110506</enddate><creator>Datta, Sumitra</creator><creator>Mody, Kalpana</creator><creator>Gopalsamy, Gnanasekaran</creator><creator>Jha, Bhavanath</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110506</creationdate><title>Novel application of κ-carrageenan: As a gelling agent in microbiological media to study biodiversity of extreme alkaliphiles</title><author>Datta, Sumitra ; Mody, Kalpana ; Gopalsamy, Gnanasekaran ; Jha, Bhavanath</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-60273f8352410f315615d45eb04363f810f07c7f148cd5708707ff241e61ecdd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Alkaliphiles</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gelling agent</topic><topic>Kappaphycus alvarezii</topic><topic>Microbiological media</topic><topic>Microbiology</topic><topic>κ-Carrageenan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Datta, Sumitra</creatorcontrib><creatorcontrib>Mody, Kalpana</creatorcontrib><creatorcontrib>Gopalsamy, Gnanasekaran</creatorcontrib><creatorcontrib>Jha, Bhavanath</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Datta, Sumitra</au><au>Mody, Kalpana</au><au>Gopalsamy, Gnanasekaran</au><au>Jha, Bhavanath</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel application of κ-carrageenan: As a gelling agent in microbiological media to study biodiversity of extreme alkaliphiles</atitle><jtitle>Carbohydrate polymers</jtitle><date>2011-05-06</date><risdate>2011</risdate><volume>85</volume><issue>2</issue><spage>465</spage><epage>468</epage><pages>465-468</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>In the present investigation a novel application of κ-carrageenan, as a gelling agent for isolation, purification and identification of extreme alkaliphilic bacteria has been explored. The potassium salt of κ-carrageenan was an appropriate substitute of agar for preparation of solid medium, particularly for isolation of extreme alkaliphiles, as agar de-polymerizes at pH
>
12.5. The purpose of formulating a new, stable, highly alkaline, solid medium was to study the diversity of extreme alkaliphilic bacteria by means of conventional cultivation techniques allied with molecular tools. The medium having pH in the range of 8.0–13.5 was used to isolate extreme alkaliphiles in pure form.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.carbpol.2011.02.036</doi><tpages>4</tpages></addata></record> |
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| ispartof | Carbohydrate polymers, 2011-05, Vol.85 (2), p.465-468 |
| issn | 0144-8617 1879-1344 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings |
| subjects | Alkaliphiles Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Fundamental and applied biological sciences. Psychology Gelling agent Kappaphycus alvarezii Microbiological media Microbiology κ-Carrageenan |
| title | Novel application of κ-carrageenan: As a gelling agent in microbiological media to study biodiversity of extreme alkaliphiles |
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