Expression of the Tick-borne Encephalitis Virus NS1 Gene in Gram-Negative Bacteria from the Mouse Nasopharynx
Bacteria were isolated from the nasopharynx of BALB/c mice and electroporated with pUR290(NS1)^sub 2^ containing two copies of tick-borne encephalitis virus (TBEV, strain Sofjin) NS1 under the control of the lac promoter. The plasmid persisted in transformants for at least ten passages. The NS1 gene...
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| Veröffentlicht in: | Molecular biology (New York) 2001-01, Vol.35 (1), p.139-143 |
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| creator | Morozova, O V Maksimova, T G Popova, R V Martsinkevich, O N Bakhvalova, V N |
| description | Bacteria were isolated from the nasopharynx of BALB/c mice and electroporated with pUR290(NS1)^sub 2^ containing two copies of tick-borne encephalitis virus (TBEV, strain Sofjin) NS1 under the control of the lac promoter. The plasmid persisted in transformants for at least ten passages. The NS1 gene expression was detected in Gram-negative enterobacteria by immunoblotting with monoclonal antibodies against the TBEV nonstructural glycoprotein NS1. Recombinant NS1 was detected in bacterial cells and in the culture medium. Intranasal immunization with recombinant bacteria caused antibodies against NS1 to appear in the serum of BALB/c mice. However, the humoral immune response to NS1 failed to protect mice from a TBEV challenge.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1023/A:1004875406855 |
| format | Article |
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The plasmid persisted in transformants for at least ten passages. The NS1 gene expression was detected in Gram-negative enterobacteria by immunoblotting with monoclonal antibodies against the TBEV nonstructural glycoprotein NS1. Recombinant NS1 was detected in bacterial cells and in the culture medium. Intranasal immunization with recombinant bacteria caused antibodies against NS1 to appear in the serum of BALB/c mice. However, the humoral immune response to NS1 failed to protect mice from a TBEV challenge.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0026-8933</identifier><identifier>EISSN: 1608-3245</identifier><identifier>DOI: 10.1023/A:1004875406855</identifier><language>eng</language><publisher>New York: Springer Nature B.V</publisher><subject>Bacteria ; Cell culture ; Glycoproteins ; Gram-negative bacteria ; Immune response (humoral) ; Immunization ; Immunoblotting ; Microbiology ; Monoclonal antibodies ; Nasopharynx ; NS1 protein ; Plasmids ; Promoters ; Tick-borne encephalitis ; Tick-borne encephalitis virus</subject><ispartof>Molecular biology (New York), 2001-01, Vol.35 (1), p.139-143</ispartof><rights>MAIK "Nauka/Interperiodica" 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Morozova, O V</creatorcontrib><creatorcontrib>Maksimova, T G</creatorcontrib><creatorcontrib>Popova, R V</creatorcontrib><creatorcontrib>Martsinkevich, O N</creatorcontrib><creatorcontrib>Bakhvalova, V N</creatorcontrib><title>Expression of the Tick-borne Encephalitis Virus NS1 Gene in Gram-Negative Bacteria from the Mouse Nasopharynx</title><title>Molecular biology (New York)</title><description>Bacteria were isolated from the nasopharynx of BALB/c mice and electroporated with pUR290(NS1)^sub 2^ containing two copies of tick-borne encephalitis virus (TBEV, strain Sofjin) NS1 under the control of the lac promoter. 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| subjects | Bacteria Cell culture Glycoproteins Gram-negative bacteria Immune response (humoral) Immunization Immunoblotting Microbiology Monoclonal antibodies Nasopharynx NS1 protein Plasmids Promoters Tick-borne encephalitis Tick-borne encephalitis virus |
| title | Expression of the Tick-borne Encephalitis Virus NS1 Gene in Gram-Negative Bacteria from the Mouse Nasopharynx |
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