Monocytes from HTLV-1–infected patients are unable to fully mature into dendritic cells

Human T-cell lymphotropic virus type 1 (HTLV-1) is a causative agent of adult T-cell leukemia and HTLV-1–associated myelopathy/tropical spastic paraparesis. HTLV-1–associated myelopathy/tropical spastic paraparesis is a chronic inflammatory disease characterized by loss of motor movement in response...

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Veröffentlicht in:Blood 2011-01, Vol.117 (2), p.489-499
Hauptverfasser: Nascimento, Clarissa Rodrigues, Lima, Marco Antonio, de Andrada Serpa, Maria José, Espindola, Otávio, Leite, Ana Claudia Celestino, Echevarria-Lima, Juliana
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container_end_page 499
container_issue 2
container_start_page 489
container_title Blood
container_volume 117
creator Nascimento, Clarissa Rodrigues
Lima, Marco Antonio
de Andrada Serpa, Maria José
Espindola, Otávio
Leite, Ana Claudia Celestino
Echevarria-Lima, Juliana
description Human T-cell lymphotropic virus type 1 (HTLV-1) is a causative agent of adult T-cell leukemia and HTLV-1–associated myelopathy/tropical spastic paraparesis. HTLV-1–associated myelopathy/tropical spastic paraparesis is a chronic inflammatory disease characterized by loss of motor movement in response to spinal marrow cell destruction by T lymphocytes. To perform their cellular function, T cells need to be activated by antigen-presenting cells, such as dendritic cells (DCs). The aim of this work was to analyze DC differentiation and activation from monocytes of HTLV-1–infected individuals. We demonstrated that monocytes from HTLV-1–infected patients who had been stimulated to differentiate had an impaired loss of CD14 expression, expressed low levels of CD1a, and maintained secretion of tumor necrosis factor-α compared with monocytes from noninfected donors. We further evaluated DC activation by tumor necrosis factor-α. We observed that in response to activation, DCs that were derived from noninfected donors had an increase in the percentage of CD83+, CD86+, and human leukocyte antigen-DR+ cells, whereas in DCs derived from HTLV-1–infected patients, the percentage of CD83+, CD86+, and human leukocyte antigen-DR+ cells remained similar to that of nonactivated cells. Moreover, these cells had an impaired capacity to stimulate allogeneic T lymphocytes. We demonstrated that DC maturation was altered in HTLV-1–infected patients, which could contribute to the development of HTLV-1–associated diseases.
doi_str_mv 10.1182/blood-2010-03-272690
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subjects Adult
Aged
Biological and medical sciences
Cell Differentiation
Cell Separation
Cytokines - biosynthesis
Deltaretrovirus Infections - immunology
Dendritic Cells - cytology
Dendritic Cells - immunology
Female
Flow Cytometry
Hematologic and hematopoietic diseases
Human T-lymphotropic virus
Human T-lymphotropic virus 1
Humans
Lymphocyte Culture Test, Mixed
Male
Medical sciences
Middle Aged
Monocytes - cytology
Monocytes - immunology
Polymerase Chain Reaction
Young Adult
title Monocytes from HTLV-1–infected patients are unable to fully mature into dendritic cells
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