Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo
Osmotic stress associated with drought or salinity is a major factor that limits plant productivity. Protein kinases in the SNF1-related protein kinase 2 (SnRK2) family are activated by osmotic stress, suggesting that the kinases are involved in osmotic stress signaling. However, due to functional r...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2011-01, Vol.108 (4), p.1717-1722 |
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| creator | Fujii, Hiroaki Verslues, Paul E Zhu, Jian-Kang |
| description | Osmotic stress associated with drought or salinity is a major factor that limits plant productivity. Protein kinases in the SNF1-related protein kinase 2 (SnRK2) family are activated by osmotic stress, suggesting that the kinases are involved in osmotic stress signaling. However, due to functional redundancy, their contribution to osmotic stress responses remained unclear. In this report, we constructed an Arabidopsis line carrying mutations in all 10 members of the SnRK2 family. The decuple mutant snrk2.1/2/3/4/5/6/7/8/9/10 grew poorly under hyperosmotic stress conditions but was similar to the wild type in culture media in the absence of osmotic stress. The mutant was also defective in gene regulation and the accumulation of abscisic acid (ABA), proline, and inositol 1,4,5-trisphosphate under osmotic stress. In addition, analysis of mutants defective in the ABA-activated SnRK2s (snrk2.2/3/6) and mutants defective in the rest of the SnRK2s (snrk2.1/4/5/7/8/9/10) revealed that SnRK2s are a merging point of ABA-dependent and -independent pathways for osmotic stress responses. These results demonstrate critical functions of the SnRK2s in mediating osmotic stress signaling and tolerance. |
| doi_str_mv | 10.1073/pnas.1018367108 |
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Protein kinases in the SNF1-related protein kinase 2 (SnRK2) family are activated by osmotic stress, suggesting that the kinases are involved in osmotic stress signaling. However, due to functional redundancy, their contribution to osmotic stress responses remained unclear. In this report, we constructed an Arabidopsis line carrying mutations in all 10 members of the SnRK2 family. The decuple mutant snrk2.1/2/3/4/5/6/7/8/9/10 grew poorly under hyperosmotic stress conditions but was similar to the wild type in culture media in the absence of osmotic stress. The mutant was also defective in gene regulation and the accumulation of abscisic acid (ABA), proline, and inositol 1,4,5-trisphosphate under osmotic stress. In addition, analysis of mutants defective in the ABA-activated SnRK2s (snrk2.2/3/6) and mutants defective in the rest of the SnRK2s (snrk2.1/4/5/7/8/9/10) revealed that SnRK2s are a merging point of ABA-dependent and -independent pathways for osmotic stress responses. These results demonstrate critical functions of the SnRK2s in mediating osmotic stress signaling and tolerance.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1018367108</identifier><identifier>PMID: 21220313</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Abscisic Acid - pharmacology ; Amino Acid Sequence ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Biological Sciences ; Dehydration ; Drought ; Droughts ; Enzyme Activation - drug effects ; Flowers & plants ; Gene expression ; Gene Expression Profiling ; Gene expression regulation ; Genetic mutation ; Inositol 1,4,5-Trisphosphate - metabolism ; Kinases ; Liquids ; Molecular Sequence Data ; Mutants ; Mutation ; Oligonucleotide Array Sequence Analysis ; Osmotic Pressure - physiology ; Oxidative stress ; Plant cells ; Plant Growth Regulators - pharmacology ; Plants ; Proline - metabolism ; Protein Kinases - genetics ; Protein Kinases - metabolism ; Protein-Serine-Threonine Kinases - genetics ; Protein-Serine-Threonine Kinases - metabolism ; Proteins ; Reverse Transcriptase Polymerase Chain Reaction ; Salinity ; Seedlings ; Sequence Homology, Amino Acid ; Signal Transduction - physiology ; Stress</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2011-01, Vol.108 (4), p.1717-1722</ispartof><rights>Copyright © 1993-2008 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Jan 25, 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-6b8a8b7c8e3806c77629071460bb486ab07e8d8a09408e523fa0d4fca9eb60f73</citedby><cites>FETCH-LOGICAL-c520t-6b8a8b7c8e3806c77629071460bb486ab07e8d8a09408e523fa0d4fca9eb60f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/108/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41001926$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41001926$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21220313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujii, Hiroaki</creatorcontrib><creatorcontrib>Verslues, Paul E</creatorcontrib><creatorcontrib>Zhu, Jian-Kang</creatorcontrib><title>Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Osmotic stress associated with drought or salinity is a major factor that limits plant productivity. Protein kinases in the SNF1-related protein kinase 2 (SnRK2) family are activated by osmotic stress, suggesting that the kinases are involved in osmotic stress signaling. However, due to functional redundancy, their contribution to osmotic stress responses remained unclear. In this report, we constructed an Arabidopsis line carrying mutations in all 10 members of the SnRK2 family. The decuple mutant snrk2.1/2/3/4/5/6/7/8/9/10 grew poorly under hyperosmotic stress conditions but was similar to the wild type in culture media in the absence of osmotic stress. The mutant was also defective in gene regulation and the accumulation of abscisic acid (ABA), proline, and inositol 1,4,5-trisphosphate under osmotic stress. In addition, analysis of mutants defective in the ABA-activated SnRK2s (snrk2.2/3/6) and mutants defective in the rest of the SnRK2s (snrk2.1/4/5/7/8/9/10) revealed that SnRK2s are a merging point of ABA-dependent and -independent pathways for osmotic stress responses. These results demonstrate critical functions of the SnRK2s in mediating osmotic stress signaling and tolerance.</description><subject>Abscisic Acid - pharmacology</subject><subject>Amino Acid Sequence</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biological Sciences</subject><subject>Dehydration</subject><subject>Drought</subject><subject>Droughts</subject><subject>Enzyme Activation - drug effects</subject><subject>Flowers & plants</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene expression regulation</subject><subject>Genetic mutation</subject><subject>Inositol 1,4,5-Trisphosphate - metabolism</subject><subject>Kinases</subject><subject>Liquids</subject><subject>Molecular Sequence Data</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Osmotic Pressure - physiology</subject><subject>Oxidative stress</subject><subject>Plant cells</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plants</subject><subject>Proline - metabolism</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>Protein-Serine-Threonine Kinases - genetics</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Proteins</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Salinity</subject><subject>Seedlings</subject><subject>Sequence Homology, Amino Acid</subject><subject>Signal Transduction - physiology</subject><subject>Stress</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EokNhzQqw2LAKvX7Ejw1SVfESlZAoXVuO47QeJnGwk5H49zjKMC1sWNny-e651_cg9JzAWwKSnY2DzeVGFBOSgHqANgQ0qQTX8BBtAKisFKf8BD3JeQsAulbwGJ1QQikwwjaoO0-2CW0cc8i49W4edx7382SHCSe_93aX8XTrcejHmMqr8zh2-Gr49oXiH6F09xmHAcfcxyk4nKfkcy6VeYzDQduHfXyKHnXFyj87nKfo-sP77xefqsuvHz9fnF9WrqYwVaJRVjXSKc8UCCeloBok4QKahithG5BetcqC5qB8TVlnoeWds9o3AjrJTtG71Xecm963zg9TsjszptDb9MtEG8zfyhBuzU3cGwZUSyGKwZuDQYo_Z58n04fs_G5nBx_nbDRwLnjN_08qLmtda7qQr_8ht3FOQ9lDgRQQUrACna2QSzHn5Lvj0ATMkrVZsjZ3WZeKl_f_euT_hHsPWCrv7JThhkiybOvFCmzzFNOR4ASArIO_WvXORmNvUsjm-ooCYUVmvAZgvwHxBMMI</recordid><startdate>20110125</startdate><enddate>20110125</enddate><creator>Fujii, Hiroaki</creator><creator>Verslues, Paul E</creator><creator>Zhu, Jian-Kang</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7ST</scope><scope>7U6</scope><scope>5PM</scope></search><sort><creationdate>20110125</creationdate><title>Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo</title><author>Fujii, Hiroaki ; Verslues, Paul E ; Zhu, Jian-Kang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-6b8a8b7c8e3806c77629071460bb486ab07e8d8a09408e523fa0d4fca9eb60f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Abscisic Acid - pharmacology</topic><topic>Amino Acid Sequence</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biological Sciences</topic><topic>Dehydration</topic><topic>Drought</topic><topic>Droughts</topic><topic>Enzyme Activation - drug effects</topic><topic>Flowers & plants</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene expression regulation</topic><topic>Genetic mutation</topic><topic>Inositol 1,4,5-Trisphosphate - metabolism</topic><topic>Kinases</topic><topic>Liquids</topic><topic>Molecular Sequence Data</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Osmotic Pressure - physiology</topic><topic>Oxidative stress</topic><topic>Plant cells</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plants</topic><topic>Proline - metabolism</topic><topic>Protein Kinases - genetics</topic><topic>Protein Kinases - metabolism</topic><topic>Protein-Serine-Threonine Kinases - genetics</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Proteins</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Salinity</topic><topic>Seedlings</topic><topic>Sequence Homology, Amino Acid</topic><topic>Signal Transduction - physiology</topic><topic>Stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujii, Hiroaki</creatorcontrib><creatorcontrib>Verslues, Paul E</creatorcontrib><creatorcontrib>Zhu, Jian-Kang</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujii, Hiroaki</au><au>Verslues, Paul E</au><au>Zhu, Jian-Kang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2011-01-25</date><risdate>2011</risdate><volume>108</volume><issue>4</issue><spage>1717</spage><epage>1722</epage><pages>1717-1722</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Osmotic stress associated with drought or salinity is a major factor that limits plant productivity. Protein kinases in the SNF1-related protein kinase 2 (SnRK2) family are activated by osmotic stress, suggesting that the kinases are involved in osmotic stress signaling. However, due to functional redundancy, their contribution to osmotic stress responses remained unclear. In this report, we constructed an Arabidopsis line carrying mutations in all 10 members of the SnRK2 family. The decuple mutant snrk2.1/2/3/4/5/6/7/8/9/10 grew poorly under hyperosmotic stress conditions but was similar to the wild type in culture media in the absence of osmotic stress. The mutant was also defective in gene regulation and the accumulation of abscisic acid (ABA), proline, and inositol 1,4,5-trisphosphate under osmotic stress. In addition, analysis of mutants defective in the ABA-activated SnRK2s (snrk2.2/3/6) and mutants defective in the rest of the SnRK2s (snrk2.1/4/5/7/8/9/10) revealed that SnRK2s are a merging point of ABA-dependent and -independent pathways for osmotic stress responses. These results demonstrate critical functions of the SnRK2s in mediating osmotic stress signaling and tolerance.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>21220313</pmid><doi>10.1073/pnas.1018367108</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Abscisic Acid - pharmacology Amino Acid Sequence Arabidopsis Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Biological Sciences Dehydration Drought Droughts Enzyme Activation - drug effects Flowers & plants Gene expression Gene Expression Profiling Gene expression regulation Genetic mutation Inositol 1,4,5-Trisphosphate - metabolism Kinases Liquids Molecular Sequence Data Mutants Mutation Oligonucleotide Array Sequence Analysis Osmotic Pressure - physiology Oxidative stress Plant cells Plant Growth Regulators - pharmacology Plants Proline - metabolism Protein Kinases - genetics Protein Kinases - metabolism Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - metabolism Proteins Reverse Transcriptase Polymerase Chain Reaction Salinity Seedlings Sequence Homology, Amino Acid Signal Transduction - physiology Stress |
| title | Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo |
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