Light-Regulated mRNA Condensation by a Photosensitive Surfactant Works as a Series Photoswitch of Translation Activity in the Presence of Small RNAs
AzoTAB, a photosensitive azobenzene cationic surfactant, which phototriggers translation activity through light-regulated condensation of mRNA, is added to a translation solution containing several mRNAs, which can be selectively silenced by specific small RNAs. We find that gene silencing by small...
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| Veröffentlicht in: | Biomacromolecules 2011-11, Vol.12 (11), p.3945-3951 |
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| container_title | Biomacromolecules |
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| creator | Rudiuk, Sergii Saito, Hirohide Hara, Tomoaki Inoue, Tan Yoshikawa, Kenichi Baigl, Damien |
| description | AzoTAB, a photosensitive azobenzene cationic surfactant, which phototriggers translation activity through light-regulated condensation of mRNA, is added to a translation solution containing several mRNAs, which can be selectively silenced by specific small RNAs. We find that gene silencing by small RNAs remains functional regardless of AzoTAB concentration and UV illumination. In the absence of UV, the translation of all genes present in the medium is partially to fully inhibited depending on AzoTAB concentration. In contrast, the application of a short UV stimulus (365 nm for 1.5 min) results in the selective photoactivation of genes that are not silenced by small RNA. These results show that light-regulated condensation by AzoTAB works as a sequence-independent series photoswitch added to parallel sequence-specific regulation by small RNAs. |
| doi_str_mv | 10.1021/bm200962s |
| format | Article |
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Psychology</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Molecular and cellular biology</subject><subject>Nucleic Acid Conformation</subject><subject>Protein Biosynthesis</subject><subject>RNA Interference</subject><subject>RNA, Messenger</subject><subject>RNA, Small Interfering - chemistry</subject><subject>Surface-Active Agents - chemistry</subject><subject>Surface-Active Agents - radiation effects</subject><subject>Ultraviolet Rays</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90c1u1DAQB_AIUdEPOPACyBdEOQRsx3bi42pFodIKqm4Rx8h2Jl2XJC4eh2rfgwfGZZfCASFZsmX9_B95piieM_qGUc7e2pFTqhXHR8URk1yVQlH--NdZlnWt68PiGPGGZlQJ-aQ45EzLhuvqqPix8tebVF7C9TyYBB0ZLz8uyDJMHUxokg8TsVtiyMUmpID5zif_Hch6jr1xyUyJfAnxKxKTF1lD9IB7e-eT25DQk6toJhx2WQuXn_u0JX4iaQPkIkIOdXDv1qMZBpLL49PioDcDwrP9flJ8Pnt3tfxQrj69P18uVqURokolMNYw0Ep0RlBLtW2gUrQBK1Wtmz5_tutopVjNLPSKOWEYSCpqa62UvOurk-LVLvc2hm8zYGpHjw6GwUwQZmw1FZRxrVSWp_-VudOi0g1jItPXO-piQIzQt7fRjyZuW0bb-3G1D-PK9sU-drYjdA_y93wyeLkHBp0Z-txK5_GPE7USWv7ljMP2Jsxxyn37R8GfNdWpfQ</recordid><startdate>20111114</startdate><enddate>20111114</enddate><creator>Rudiuk, Sergii</creator><creator>Saito, Hirohide</creator><creator>Hara, Tomoaki</creator><creator>Inoue, Tan</creator><creator>Yoshikawa, Kenichi</creator><creator>Baigl, Damien</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20111114</creationdate><title>Light-Regulated mRNA Condensation by a Photosensitive Surfactant Works as a Series Photoswitch of Translation Activity in the Presence of Small RNAs</title><author>Rudiuk, Sergii ; Saito, Hirohide ; Hara, Tomoaki ; Inoue, Tan ; Yoshikawa, Kenichi ; Baigl, Damien</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a443t-e1181e964da40b09b8e3608eb56798f093dd036171bef61c4a1e5047bbb552df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Azo Compounds - chemistry</topic><topic>Azo Compounds - radiation effects</topic><topic>Biological and medical sciences</topic><topic>Diverse techniques</topic><topic>Fundamental and applied biological sciences. 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| subjects | Azo Compounds - chemistry Azo Compounds - radiation effects Biological and medical sciences Diverse techniques Fundamental and applied biological sciences. Psychology Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Molecular and cellular biology Nucleic Acid Conformation Protein Biosynthesis RNA Interference RNA, Messenger RNA, Small Interfering - chemistry Surface-Active Agents - chemistry Surface-Active Agents - radiation effects Ultraviolet Rays |
| title | Light-Regulated mRNA Condensation by a Photosensitive Surfactant Works as a Series Photoswitch of Translation Activity in the Presence of Small RNAs |
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