Deficiency of CX3CR1 delays burn wound healing and is associated with reduced myeloid cell recruitment and decreased sub-dermal angiogenesis
Abstract The development of a good blood supply is a key step in burn wound healing and appears to be regulated in part by myeloid cells. CX3CR1 positive cells have recently been identified as myeloid cells with a potential role in angiogenesis. The role of functional CX3CR1 system in burn wound hea...
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| Veröffentlicht in: | Burns 2011-12, Vol.37 (8), p.1386-1393 |
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| creator | Clover, Anthony J.P Kumar, Arun H.S Caplice, Noel M |
| description | Abstract The development of a good blood supply is a key step in burn wound healing and appears to be regulated in part by myeloid cells. CX3CR1 positive cells have recently been identified as myeloid cells with a potential role in angiogenesis. The role of functional CX3CR1 system in burn wound healing is not previously investigated. A 2% contact burn was induced in CX3CR1+/gfp and CX3CR1gfp/gfp mice. These transgenic mice facilitate the tracking of CX3CR1 cells (CX3CR1+/gfp ) and allow evaluation of the consequence of CX3CR1 functional knockout (CX3CR1gfp/gfp ) on burn wound healing. The progression of wound healing was monitored before tissue was harvested and analyzed at day 6 and day 12 for migration of CX3CR1 cells into burn wound. Deficiency of a functional CX3CR1 system resulted in decreased recruitment of CX3CR1 positive cells into the burn wound associated with decreased myeloid cell recruitment ( p < 0.001) and reduced maintenance of new vessels ( p < 0.001). Burn wound healing was prolonged ( p < 0.05). Our study is the first to establish a role for CX3CR1 in burn wound healing which is associated with sub-dermal angiogenesis. This chemokine receptor pathway may be attractive for therapeutic manipulation as it could increase sub dermal angiogenesis and thereby improve time to healing. |
| doi_str_mv | 10.1016/j.burns.2011.08.001 |
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CX3CR1 positive cells have recently been identified as myeloid cells with a potential role in angiogenesis. The role of functional CX3CR1 system in burn wound healing is not previously investigated. A 2% contact burn was induced in CX3CR1+/gfp and CX3CR1gfp/gfp mice. These transgenic mice facilitate the tracking of CX3CR1 cells (CX3CR1+/gfp ) and allow evaluation of the consequence of CX3CR1 functional knockout (CX3CR1gfp/gfp ) on burn wound healing. The progression of wound healing was monitored before tissue was harvested and analyzed at day 6 and day 12 for migration of CX3CR1 cells into burn wound. Deficiency of a functional CX3CR1 system resulted in decreased recruitment of CX3CR1 positive cells into the burn wound associated with decreased myeloid cell recruitment ( p < 0.001) and reduced maintenance of new vessels ( p < 0.001). Burn wound healing was prolonged ( p < 0.05). Our study is the first to establish a role for CX3CR1 in burn wound healing which is associated with sub-dermal angiogenesis. This chemokine receptor pathway may be attractive for therapeutic manipulation as it could increase sub dermal angiogenesis and thereby improve time to healing.</description><identifier>ISSN: 0305-4179</identifier><identifier>EISSN: 1879-1409</identifier><identifier>DOI: 10.1016/j.burns.2011.08.001</identifier><identifier>PMID: 21924836</identifier><identifier>CODEN: BURND8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Animals ; Biological and medical sciences ; Burn healing ; Burns ; Burns - metabolism ; Burns - pathology ; Burns - physiopathology ; Chemokine CX3CL1 - metabolism ; Critical Care ; CX3C Chemokine Receptor 1 ; CX3CR1 ; Endothelial Cells - pathology ; Immunohistochemistry ; Macrophages ; Macrophages - pathology ; Medical sciences ; Mice ; Mice, Transgenic ; Models, Animal ; Monocytes ; Myeloid Cells - pathology ; Neovascularization, Physiologic - physiology ; Receptors, Chemokine - deficiency ; Skin - blood supply ; Skin - cytology ; Traumas. Diseases due to physical agents ; Wound healing ; Wound Healing - physiology</subject><ispartof>Burns, 2011-12, Vol.37 (8), p.1386-1393</ispartof><rights>Elsevier Ltd and ISBI</rights><rights>2011 Elsevier Ltd and ISBI</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Ltd and ISBI. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-9d3e08325585d5d8cb74044f66a8e2d0671fc6e70c2478290c28cc330d91198a3</citedby><cites>FETCH-LOGICAL-c443t-9d3e08325585d5d8cb74044f66a8e2d0671fc6e70c2478290c28cc330d91198a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0305417911002397$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24781650$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21924836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clover, Anthony J.P</creatorcontrib><creatorcontrib>Kumar, Arun H.S</creatorcontrib><creatorcontrib>Caplice, Noel M</creatorcontrib><title>Deficiency of CX3CR1 delays burn wound healing and is associated with reduced myeloid cell recruitment and decreased sub-dermal angiogenesis</title><title>Burns</title><addtitle>Burns</addtitle><description>Abstract The development of a good blood supply is a key step in burn wound healing and appears to be regulated in part by myeloid cells. CX3CR1 positive cells have recently been identified as myeloid cells with a potential role in angiogenesis. The role of functional CX3CR1 system in burn wound healing is not previously investigated. A 2% contact burn was induced in CX3CR1+/gfp and CX3CR1gfp/gfp mice. These transgenic mice facilitate the tracking of CX3CR1 cells (CX3CR1+/gfp ) and allow evaluation of the consequence of CX3CR1 functional knockout (CX3CR1gfp/gfp ) on burn wound healing. The progression of wound healing was monitored before tissue was harvested and analyzed at day 6 and day 12 for migration of CX3CR1 cells into burn wound. Deficiency of a functional CX3CR1 system resulted in decreased recruitment of CX3CR1 positive cells into the burn wound associated with decreased myeloid cell recruitment ( p < 0.001) and reduced maintenance of new vessels ( p < 0.001). Burn wound healing was prolonged ( p < 0.05). Our study is the first to establish a role for CX3CR1 in burn wound healing which is associated with sub-dermal angiogenesis. This chemokine receptor pathway may be attractive for therapeutic manipulation as it could increase sub dermal angiogenesis and thereby improve time to healing.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Burn healing</subject><subject>Burns</subject><subject>Burns - metabolism</subject><subject>Burns - pathology</subject><subject>Burns - physiopathology</subject><subject>Chemokine CX3CL1 - metabolism</subject><subject>Critical Care</subject><subject>CX3C Chemokine Receptor 1</subject><subject>CX3CR1</subject><subject>Endothelial Cells - pathology</subject><subject>Immunohistochemistry</subject><subject>Macrophages</subject><subject>Macrophages - pathology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Models, Animal</subject><subject>Monocytes</subject><subject>Myeloid Cells - pathology</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Receptors, Chemokine - deficiency</subject><subject>Skin - blood supply</subject><subject>Skin - cytology</subject><subject>Traumas. Diseases due to physical agents</subject><subject>Wound healing</subject><subject>Wound Healing - physiology</subject><issn>0305-4179</issn><issn>1879-1409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFktuKFDEQhhtR3NnVJxAkN-JVj5VOH9IXCjKuB1gQPIB3IZNUz2bsw26q26XfwYe2emZU8Mbc5PT9leL_kyRPJKwlyPLFfr2dYk_rDKRcg14DyHvJSuqqTmUO9f1kBQqKNJdVfZacE-2BR6HhYXKWyTrLtSpXyc832AQXsHezGBqx-aY2n6Tw2NqZxFJf3A1T78U12jb0O2F5HUhYosEFO6IXd2G8FhH95HjTzdgOwQuHbcuHLk5h7LAfDzrPe7TEGE3b1GPsbMsXuzDssEcK9Ch50NiW8PFpvki-vr38snmfXn1892Hz-ip1ea7GtPYKQausKHThC6_dtsohz5uytBozD2UlG1diBS7LK53VPGvnlAJfS1lrqy6S58e6N3G4nZBG0wVaWrY9DhOZGnL2tJIVk-pIujgQRWzMTQydjbORYJYUzN4cUjBLCga04RRY9fRUf9p26P9oftvOwLMTYMnZtom2d4H-cty2LAtg7uWRQ3bjR8Bo6JAV-sDmjsYP4T-NvPpH7zjFwE9-xxlpP7CCjTbSUGbAfF4-zPJfpATIVF2pXwcOvD8</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Clover, Anthony J.P</creator><creator>Kumar, Arun H.S</creator><creator>Caplice, Noel M</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111201</creationdate><title>Deficiency of CX3CR1 delays burn wound healing and is associated with reduced myeloid cell recruitment and decreased sub-dermal angiogenesis</title><author>Clover, Anthony J.P ; Kumar, Arun H.S ; Caplice, Noel M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-9d3e08325585d5d8cb74044f66a8e2d0671fc6e70c2478290c28cc330d91198a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Burn healing</topic><topic>Burns</topic><topic>Burns - metabolism</topic><topic>Burns - pathology</topic><topic>Burns - physiopathology</topic><topic>Chemokine CX3CL1 - metabolism</topic><topic>Critical Care</topic><topic>CX3C Chemokine Receptor 1</topic><topic>CX3CR1</topic><topic>Endothelial Cells - pathology</topic><topic>Immunohistochemistry</topic><topic>Macrophages</topic><topic>Macrophages - pathology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Models, Animal</topic><topic>Monocytes</topic><topic>Myeloid Cells - pathology</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Receptors, Chemokine - deficiency</topic><topic>Skin - blood supply</topic><topic>Skin - cytology</topic><topic>Traumas. Diseases due to physical agents</topic><topic>Wound healing</topic><topic>Wound Healing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clover, Anthony J.P</creatorcontrib><creatorcontrib>Kumar, Arun H.S</creatorcontrib><creatorcontrib>Caplice, Noel M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Burns</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clover, Anthony J.P</au><au>Kumar, Arun H.S</au><au>Caplice, Noel M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deficiency of CX3CR1 delays burn wound healing and is associated with reduced myeloid cell recruitment and decreased sub-dermal angiogenesis</atitle><jtitle>Burns</jtitle><addtitle>Burns</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>37</volume><issue>8</issue><spage>1386</spage><epage>1393</epage><pages>1386-1393</pages><issn>0305-4179</issn><eissn>1879-1409</eissn><coden>BURND8</coden><abstract>Abstract The development of a good blood supply is a key step in burn wound healing and appears to be regulated in part by myeloid cells. CX3CR1 positive cells have recently been identified as myeloid cells with a potential role in angiogenesis. The role of functional CX3CR1 system in burn wound healing is not previously investigated. A 2% contact burn was induced in CX3CR1+/gfp and CX3CR1gfp/gfp mice. These transgenic mice facilitate the tracking of CX3CR1 cells (CX3CR1+/gfp ) and allow evaluation of the consequence of CX3CR1 functional knockout (CX3CR1gfp/gfp ) on burn wound healing. The progression of wound healing was monitored before tissue was harvested and analyzed at day 6 and day 12 for migration of CX3CR1 cells into burn wound. Deficiency of a functional CX3CR1 system resulted in decreased recruitment of CX3CR1 positive cells into the burn wound associated with decreased myeloid cell recruitment ( p < 0.001) and reduced maintenance of new vessels ( p < 0.001). Burn wound healing was prolonged ( p < 0.05). Our study is the first to establish a role for CX3CR1 in burn wound healing which is associated with sub-dermal angiogenesis. This chemokine receptor pathway may be attractive for therapeutic manipulation as it could increase sub dermal angiogenesis and thereby improve time to healing.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21924836</pmid><doi>10.1016/j.burns.2011.08.001</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Burn healing Burns Burns - metabolism Burns - pathology Burns - physiopathology Chemokine CX3CL1 - metabolism Critical Care CX3C Chemokine Receptor 1 CX3CR1 Endothelial Cells - pathology Immunohistochemistry Macrophages Macrophages - pathology Medical sciences Mice Mice, Transgenic Models, Animal Monocytes Myeloid Cells - pathology Neovascularization, Physiologic - physiology Receptors, Chemokine - deficiency Skin - blood supply Skin - cytology Traumas. Diseases due to physical agents Wound healing Wound Healing - physiology |
| title | Deficiency of CX3CR1 delays burn wound healing and is associated with reduced myeloid cell recruitment and decreased sub-dermal angiogenesis |
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