Production of Recombinant Serpins in Escherichia coli

Serpins represent a diverse family of proteins that are found in a wide range of organisms and cellular locations. In order to study them, most need to be produced recombinantly, as isolation from their source is not always possible. Due to their relatively uncomplicated structure (single domain, fe...

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Bibliographische Detailangaben
Hauptverfasser:	Pearce, Mary C., Cabrita, Lisa D.
Format:	Buchkapitel
Sprache:	eng
Schlagworte:	alpha 1-Antitrypsin - biosynthesis alpha 1-Antitrypsin - genetics alpha 1-Antitrypsin - isolation & purification Antitrypsin Carbon Radioisotopes - metabolism Chromatography, Affinity - methods Cloning, Molecular - methods Culture Media Electrophoresis, Polyacrylamide Gel Enzymology Escherichia coli - classification Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Humans Inclusion Bodies - chemistry Inclusion Bodies - metabolism Molecular Biology - methods Plasmids - chemistry Plasmids - genetics Promoter Regions, Genetic Protein expression Protein Folding Protein purification Protein Refolding Proteins Recombinant protein Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Serpin Solubility Staining and Labeling - methods Therapy & therapeutics Transformation, Bacterial
Online-Zugang:	Volltext
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