Improvement of skin-graft survival after autologous transplantation of adipose-derived stem cells in rats
Summary Background Skin grafts are frequently used for a variety of indications in plastic and reconstructive surgery. Their necrosis is a common complication, while different therapies have been proposed. Currently, adipose-derived stem cells (ASCs) hold great promise for their angiogenic potential...
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| Veröffentlicht in: | Journal of plastic, reconstructive & aesthetic surgery reconstructive & aesthetic surgery, 2011-12, Vol.64 (12), p.1647-1656 |
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| creator | Zografou, A Tsigris, C Papadopoulos, O Kavantzas, N Patsouris, E Donta, I Perrea, D |
| description | Summary Background Skin grafts are frequently used for a variety of indications in plastic and reconstructive surgery. Their necrosis is a common complication, while different therapies have been proposed. Currently, adipose-derived stem cells (ASCs) hold great promise for their angiogenic potential and role during tissue repair. In this study, autologous transplantation of ASCs was used in skin grafts in rats to determine if it increases angiogenesis, skin-graft survival and wound healing. Methods ASCs were isolated, cultured, labelled with fluorescent dye and injected under full-thickness skin grafts in 10 rats (group 1), while 10 others served as controls (group 2). Skin grafts were analysed after 1 week. Collagen’s framework was assessed with Masson’s trichrome stain and angiogenesis with von Willebrand factor (vWF) immunohistochemistry. In addition, immunohistochemical staining intensity of vascular endothelial growth factor (VEGF) and transforming growth factor b3 (TGFb3) was assessed in all grafts. Results Mean area of graft necrosis was significantly less in group 1 than in group 2 (6.12% vs. 32.62%, p |
| doi_str_mv | 10.1016/j.bjps.2011.07.009 |
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Their necrosis is a common complication, while different therapies have been proposed. Currently, adipose-derived stem cells (ASCs) hold great promise for their angiogenic potential and role during tissue repair. In this study, autologous transplantation of ASCs was used in skin grafts in rats to determine if it increases angiogenesis, skin-graft survival and wound healing. Methods ASCs were isolated, cultured, labelled with fluorescent dye and injected under full-thickness skin grafts in 10 rats (group 1), while 10 others served as controls (group 2). Skin grafts were analysed after 1 week. Collagen’s framework was assessed with Masson’s trichrome stain and angiogenesis with von Willebrand factor (vWF) immunohistochemistry. In addition, immunohistochemical staining intensity of vascular endothelial growth factor (VEGF) and transforming growth factor b3 (TGFb3) was assessed in all grafts. Results Mean area of graft necrosis was significantly less in group 1 than in group 2 (6.12% vs. 32.62%, p < 0.01). Statistically significant increase of microvessel density, collagen density, VEGF and TGFb3 expression was noted in group 1 compared with group 2 (all: p < 0.01). Conclusions These findings suggest that autologous ASCs transplantation increases full-thickness skin-graft survival and shows promise for use in skin-graft surgery. This might be both due to in situ differentiation of ASCs into endothelial cells and increased secretion by ASCs of growth factors, such as VEGF and TGFb3 that enhance angiogenesis and wound healing.</description><identifier>ISSN: 1748-6815</identifier><identifier>EISSN: 1878-0539</identifier><identifier>DOI: 10.1016/j.bjps.2011.07.009</identifier><identifier>PMID: 21839697</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Adipose Tissue - cytology ; Adipose-derived stem cells ; Angiogenesis ; Animals ; Biological and medical sciences ; Cell Differentiation ; Cells, Cultured ; Flow Cytometry ; Graft Survival - physiology ; Immunohistochemistry ; Immunophenotyping ; Male ; Medical sciences ; Plastic Surgery ; Rats ; Rats, Sprague-Dawley ; Skin Transplantation - physiology ; Skin-graft ; Stem Cell Transplantation ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; TGFb3 ; Transplantation, Autologous ; Vascular Endothelial Growth Factor A - metabolism ; VEGF ; Wound Healing - physiology</subject><ispartof>Journal of plastic, reconstructive & aesthetic surgery, 2011-12, Vol.64 (12), p.1647-1656</ispartof><rights>British Association of Plastic, Reconstructive and Aesthetic Surgeons</rights><rights>2011 British Association of Plastic, Reconstructive and Aesthetic Surgeons</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-19ad81e56e9b62d3270151ab785e58ffeade23fcd289ec2281ea4aa1fbe1a12f3</citedby><cites>FETCH-LOGICAL-c440t-19ad81e56e9b62d3270151ab785e58ffeade23fcd289ec2281ea4aa1fbe1a12f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bjps.2011.07.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25245916$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21839697$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zografou, A</creatorcontrib><creatorcontrib>Tsigris, C</creatorcontrib><creatorcontrib>Papadopoulos, O</creatorcontrib><creatorcontrib>Kavantzas, N</creatorcontrib><creatorcontrib>Patsouris, E</creatorcontrib><creatorcontrib>Donta, I</creatorcontrib><creatorcontrib>Perrea, D</creatorcontrib><title>Improvement of skin-graft survival after autologous transplantation of adipose-derived stem cells in rats</title><title>Journal of plastic, reconstructive & aesthetic surgery</title><addtitle>J Plast Reconstr Aesthet Surg</addtitle><description>Summary Background Skin grafts are frequently used for a variety of indications in plastic and reconstructive surgery. Their necrosis is a common complication, while different therapies have been proposed. Currently, adipose-derived stem cells (ASCs) hold great promise for their angiogenic potential and role during tissue repair. In this study, autologous transplantation of ASCs was used in skin grafts in rats to determine if it increases angiogenesis, skin-graft survival and wound healing. Methods ASCs were isolated, cultured, labelled with fluorescent dye and injected under full-thickness skin grafts in 10 rats (group 1), while 10 others served as controls (group 2). Skin grafts were analysed after 1 week. Collagen’s framework was assessed with Masson’s trichrome stain and angiogenesis with von Willebrand factor (vWF) immunohistochemistry. In addition, immunohistochemical staining intensity of vascular endothelial growth factor (VEGF) and transforming growth factor b3 (TGFb3) was assessed in all grafts. Results Mean area of graft necrosis was significantly less in group 1 than in group 2 (6.12% vs. 32.62%, p < 0.01). Statistically significant increase of microvessel density, collagen density, VEGF and TGFb3 expression was noted in group 1 compared with group 2 (all: p < 0.01). Conclusions These findings suggest that autologous ASCs transplantation increases full-thickness skin-graft survival and shows promise for use in skin-graft surgery. This might be both due to in situ differentiation of ASCs into endothelial cells and increased secretion by ASCs of growth factors, such as VEGF and TGFb3 that enhance angiogenesis and wound healing.</description><subject>Adipose Tissue - cytology</subject><subject>Adipose-derived stem cells</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Flow Cytometry</subject><subject>Graft Survival - physiology</subject><subject>Immunohistochemistry</subject><subject>Immunophenotyping</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Plastic Surgery</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Skin Transplantation - physiology</subject><subject>Skin-graft</subject><subject>Stem Cell Transplantation</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>TGFb3</subject><subject>Transplantation, Autologous</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>VEGF</subject><subject>Wound Healing - physiology</subject><issn>1748-6815</issn><issn>1878-0539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kl2L1TAQhoso7nr0D3ghuRGvWjPpN4iwLH4sLHihgnchTSZLum16zKSF_femnKOCF14lgeedzDxMlr0EXgCH5u1YDOORCsEBCt4WnPePskvo2i7nddk_Tve26vKmg_oie0Y0cl6VUNVPswsBXdk3fXuZuZv5GJYNZ_SRLZbRvfP5XVA2MlrD5jY1sfTAwNQal2m5W1ZiMShPx0n5qKJb_J5Txh0XwtxgcBsaRhFnpnGaiDnPgor0PHti1UT44nwesu8fP3y7_pzffvl0c311m-uq4jGHXpkOsG6wHxphStFyqEENbVdj3VmLyqAorTai61ELkVhVKQV2QFAgbHnI3pzqprl-rkhRzo72TpTH1LzseZVM8aTokIkTqcNCFNDKY3CzCg8SuNwNy1HuhuVuWPJWplwKvTqXX4cZzZ_Ib6UJeH0GFGk12eRKO_rL1aKqe2gS9-7EYZKxOQyStEOv0biAOkqzuP_38f6fuJ6cd-nHe3xAGpc1-KRZgiQhufy678K-CgCcl13zo_wFMGyxfQ</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Zografou, A</creator><creator>Tsigris, C</creator><creator>Papadopoulos, O</creator><creator>Kavantzas, N</creator><creator>Patsouris, E</creator><creator>Donta, I</creator><creator>Perrea, D</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111201</creationdate><title>Improvement of skin-graft survival after autologous transplantation of adipose-derived stem cells in rats</title><author>Zografou, A ; Tsigris, C ; Papadopoulos, O ; Kavantzas, N ; Patsouris, E ; Donta, I ; Perrea, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-19ad81e56e9b62d3270151ab785e58ffeade23fcd289ec2281ea4aa1fbe1a12f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adipose Tissue - cytology</topic><topic>Adipose-derived stem cells</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Flow Cytometry</topic><topic>Graft Survival - physiology</topic><topic>Immunohistochemistry</topic><topic>Immunophenotyping</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Plastic Surgery</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Skin Transplantation - physiology</topic><topic>Skin-graft</topic><topic>Stem Cell Transplantation</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>TGFb3</topic><topic>Transplantation, Autologous</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>VEGF</topic><topic>Wound Healing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zografou, A</creatorcontrib><creatorcontrib>Tsigris, C</creatorcontrib><creatorcontrib>Papadopoulos, O</creatorcontrib><creatorcontrib>Kavantzas, N</creatorcontrib><creatorcontrib>Patsouris, E</creatorcontrib><creatorcontrib>Donta, I</creatorcontrib><creatorcontrib>Perrea, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of plastic, reconstructive & aesthetic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zografou, A</au><au>Tsigris, C</au><au>Papadopoulos, O</au><au>Kavantzas, N</au><au>Patsouris, E</au><au>Donta, I</au><au>Perrea, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improvement of skin-graft survival after autologous transplantation of adipose-derived stem cells in rats</atitle><jtitle>Journal of plastic, reconstructive & aesthetic surgery</jtitle><addtitle>J Plast Reconstr Aesthet Surg</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>64</volume><issue>12</issue><spage>1647</spage><epage>1656</epage><pages>1647-1656</pages><issn>1748-6815</issn><eissn>1878-0539</eissn><abstract>Summary Background Skin grafts are frequently used for a variety of indications in plastic and reconstructive surgery. Their necrosis is a common complication, while different therapies have been proposed. Currently, adipose-derived stem cells (ASCs) hold great promise for their angiogenic potential and role during tissue repair. In this study, autologous transplantation of ASCs was used in skin grafts in rats to determine if it increases angiogenesis, skin-graft survival and wound healing. Methods ASCs were isolated, cultured, labelled with fluorescent dye and injected under full-thickness skin grafts in 10 rats (group 1), while 10 others served as controls (group 2). Skin grafts were analysed after 1 week. Collagen’s framework was assessed with Masson’s trichrome stain and angiogenesis with von Willebrand factor (vWF) immunohistochemistry. In addition, immunohistochemical staining intensity of vascular endothelial growth factor (VEGF) and transforming growth factor b3 (TGFb3) was assessed in all grafts. Results Mean area of graft necrosis was significantly less in group 1 than in group 2 (6.12% vs. 32.62%, p < 0.01). Statistically significant increase of microvessel density, collagen density, VEGF and TGFb3 expression was noted in group 1 compared with group 2 (all: p < 0.01). Conclusions These findings suggest that autologous ASCs transplantation increases full-thickness skin-graft survival and shows promise for use in skin-graft surgery. This might be both due to in situ differentiation of ASCs into endothelial cells and increased secretion by ASCs of growth factors, such as VEGF and TGFb3 that enhance angiogenesis and wound healing.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21839697</pmid><doi>10.1016/j.bjps.2011.07.009</doi><tpages>10</tpages></addata></record> |
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| ispartof | Journal of plastic, reconstructive & aesthetic surgery, 2011-12, Vol.64 (12), p.1647-1656 |
| issn | 1748-6815 1878-0539 |
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| subjects | Adipose Tissue - cytology Adipose-derived stem cells Angiogenesis Animals Biological and medical sciences Cell Differentiation Cells, Cultured Flow Cytometry Graft Survival - physiology Immunohistochemistry Immunophenotyping Male Medical sciences Plastic Surgery Rats Rats, Sprague-Dawley Skin Transplantation - physiology Skin-graft Stem Cell Transplantation Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases TGFb3 Transplantation, Autologous Vascular Endothelial Growth Factor A - metabolism VEGF Wound Healing - physiology |
| title | Improvement of skin-graft survival after autologous transplantation of adipose-derived stem cells in rats |
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