Sensitive and cost-effective LC–MS/MS method for quantitation of CVT-6883 in human urine using sodium dodecylbenzenesulfonate additive to eliminate adsorptive losses
CVT-6883, a novel selective A 2B adenosine receptor antagonist currently under clinical development, is highly lipophilic and exhibits high affinity for non-specific binding to container surfaces, resulting in very low recovery in urine assays. Our study showed the use of sodium dodecylbenzenesulfon...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-04, Vol.877 (10), p.943-947 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Chen, Chungwen Bajpai, Lakshmikant Mollova, Nevena Leung, Kwan |
| description | CVT-6883, a novel selective A
2B adenosine receptor antagonist currently under clinical development, is highly lipophilic and exhibits high affinity for non-specific binding to container surfaces, resulting in very low recovery in urine assays. Our study showed the use of sodium dodecylbenzenesulfonate (SDBS), a low-cost additive, eliminated non-specific binding problems in the analysis of CVT-6883 in human urine without compromising sensitivity. A new sensitive and selective LC–MS/MS method for quantitation of CVT-6883 in the range of 0.200–80.0
ng/mL using SDBS additive was therefore developed and validated for the analysis of human urine samples. The recoveries during sample collection, handling and extraction for the analyte and internal standard (
d
5-CVT-6883) were higher than 87%. CVT-6883 was found stable under the following conditions: in extract – at ambient temperature for 3 days, under refrigeration (5
°C) for 6 days; in human urine (containing 4
mM SDBS) – after three freeze/thaw cycles, at ambient temperature for 26
h, under refrigeration (5
°C) for 94
h, and in a freezer set to −20
°C for at least 2 months. The results demonstrated that the validated method is sufficiently sensitive, specific, and cost-effective for the analysis of CVT-6883 in human urine and will provide a powerful tool to support the clinical programs for CVT-6883. |
| doi_str_mv | 10.1016/j.jchromb.2009.02.045 |
| format | Article |
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2B adenosine receptor antagonist currently under clinical development, is highly lipophilic and exhibits high affinity for non-specific binding to container surfaces, resulting in very low recovery in urine assays. Our study showed the use of sodium dodecylbenzenesulfonate (SDBS), a low-cost additive, eliminated non-specific binding problems in the analysis of CVT-6883 in human urine without compromising sensitivity. A new sensitive and selective LC–MS/MS method for quantitation of CVT-6883 in the range of 0.200–80.0
ng/mL using SDBS additive was therefore developed and validated for the analysis of human urine samples. The recoveries during sample collection, handling and extraction for the analyte and internal standard (
d
5-CVT-6883) were higher than 87%. CVT-6883 was found stable under the following conditions: in extract – at ambient temperature for 3 days, under refrigeration (5
°C) for 6 days; in human urine (containing 4
mM SDBS) – after three freeze/thaw cycles, at ambient temperature for 26
h, under refrigeration (5
°C) for 94
h, and in a freezer set to −20
°C for at least 2 months. The results demonstrated that the validated method is sufficiently sensitive, specific, and cost-effective for the analysis of CVT-6883 in human urine and will provide a powerful tool to support the clinical programs for CVT-6883.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2009.02.045</identifier><identifier>PMID: 19282253</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>A 2B adenosin receptor antagonist ; Adsorption ; Adsorptive losses ; Analysis ; Analytical, structural and metabolic biochemistry ; Benzenesulfonates - chemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid - economics ; Chromatography, High Pressure Liquid - methods ; CVT-6883 ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Human urine ; Humans ; LC–MS/MS ; Medical sciences ; Method validation ; Non-specific binding ; Pharmacology. Drug treatments ; Purines - urine ; Pyrazoles - urine ; Reference Standards ; SDBS ; Sodium dodecylbenzenesulfonate ; Tandem Mass Spectrometry - economics ; Tandem Mass Spectrometry - methods</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2009-04, Vol.877 (10), p.943-947</ispartof><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-9bcd67e8e895cd939c3fccdc52b1e822b3c08f9405639f65ec088f027083a2e3</citedby><cites>FETCH-LOGICAL-c485t-9bcd67e8e895cd939c3fccdc52b1e822b3c08f9405639f65ec088f027083a2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2009.02.045$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21336792$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19282253$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Chungwen</creatorcontrib><creatorcontrib>Bajpai, Lakshmikant</creatorcontrib><creatorcontrib>Mollova, Nevena</creatorcontrib><creatorcontrib>Leung, Kwan</creatorcontrib><title>Sensitive and cost-effective LC–MS/MS method for quantitation of CVT-6883 in human urine using sodium dodecylbenzenesulfonate additive to eliminate adsorptive losses</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>CVT-6883, a novel selective A
2B adenosine receptor antagonist currently under clinical development, is highly lipophilic and exhibits high affinity for non-specific binding to container surfaces, resulting in very low recovery in urine assays. Our study showed the use of sodium dodecylbenzenesulfonate (SDBS), a low-cost additive, eliminated non-specific binding problems in the analysis of CVT-6883 in human urine without compromising sensitivity. A new sensitive and selective LC–MS/MS method for quantitation of CVT-6883 in the range of 0.200–80.0
ng/mL using SDBS additive was therefore developed and validated for the analysis of human urine samples. The recoveries during sample collection, handling and extraction for the analyte and internal standard (
d
5-CVT-6883) were higher than 87%. CVT-6883 was found stable under the following conditions: in extract – at ambient temperature for 3 days, under refrigeration (5
°C) for 6 days; in human urine (containing 4
mM SDBS) – after three freeze/thaw cycles, at ambient temperature for 26
h, under refrigeration (5
°C) for 94
h, and in a freezer set to −20
°C for at least 2 months. The results demonstrated that the validated method is sufficiently sensitive, specific, and cost-effective for the analysis of CVT-6883 in human urine and will provide a powerful tool to support the clinical programs for CVT-6883.</description><subject>A 2B adenosin receptor antagonist</subject><subject>Adsorption</subject><subject>Adsorptive losses</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Benzenesulfonates - chemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - economics</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>CVT-6883</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Human urine</subject><subject>Humans</subject><subject>LC–MS/MS</subject><subject>Medical sciences</subject><subject>Method validation</subject><subject>Non-specific binding</subject><subject>Pharmacology. Drug treatments</subject><subject>Purines - urine</subject><subject>Pyrazoles - urine</subject><subject>Reference Standards</subject><subject>SDBS</subject><subject>Sodium dodecylbenzenesulfonate</subject><subject>Tandem Mass Spectrometry - economics</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks2OFCEUhStG44ytj6Bho66qhoKiClbGdPxLeuKiO8YdoeBi06mCHqiaZFz5Dj6E7-WTyExXdOesgJPvcu7lUBTPa1zVuG4vDtVB72MY-4pgLCpMKtywB8V5zTta0q79-jDvWYdLTCg5K56kdMC47nBHHxdntSCcEEbPi19b8MlN7hqQ8gbpkKYSrAV9J23Wv3_8vNxeXG7RCNM-GGRDRFez8pOb1OSCR8Gi9Zdd2XJOkfNoP4_Kozk6D2hOzn9DKRg3j8gEA_pm6MF_Bw9pHmzwasquxpzsp4BgcKNb1BTi8U4fQkqQnhaPrBoSPFvWVbF7_263_lhuPn_4tH67KXXD2VSKXpu2Aw5cMG0EFZparY1mpK8hj9xTjbkVDWYtFbZlkI_cYtJhThUBuipen649xnA1Q5rk6JKGYVAewpykwLRtOp7pVfHqv2Tb4Uawmt4L0qZpCcuprQp2AnXMI0ew8hjdqOKNrLG8zVwe5JK5vM1cYiJz5rnuxWIw9yOYf1VLyBl4uQAqaTXYqLx26S9HcpNtJ0jm3pw4yA987SDKpB14DcbF_CGkCe6eVv4AEhTQsg</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Chen, Chungwen</creator><creator>Bajpai, Lakshmikant</creator><creator>Mollova, Nevena</creator><creator>Leung, Kwan</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>7X8</scope></search><sort><creationdate>20090401</creationdate><title>Sensitive and cost-effective LC–MS/MS method for quantitation of CVT-6883 in human urine using sodium dodecylbenzenesulfonate additive to eliminate adsorptive losses</title><author>Chen, Chungwen ; Bajpai, Lakshmikant ; Mollova, Nevena ; Leung, Kwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-9bcd67e8e895cd939c3fccdc52b1e822b3c08f9405639f65ec088f027083a2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>A 2B adenosin receptor antagonist</topic><topic>Adsorption</topic><topic>Adsorptive losses</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Benzenesulfonates - chemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - economics</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>CVT-6883</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Human urine</topic><topic>Humans</topic><topic>LC–MS/MS</topic><topic>Medical sciences</topic><topic>Method validation</topic><topic>Non-specific binding</topic><topic>Pharmacology. Drug treatments</topic><topic>Purines - urine</topic><topic>Pyrazoles - urine</topic><topic>Reference Standards</topic><topic>SDBS</topic><topic>Sodium dodecylbenzenesulfonate</topic><topic>Tandem Mass Spectrometry - economics</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Chungwen</creatorcontrib><creatorcontrib>Bajpai, Lakshmikant</creatorcontrib><creatorcontrib>Mollova, Nevena</creatorcontrib><creatorcontrib>Leung, Kwan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Chungwen</au><au>Bajpai, Lakshmikant</au><au>Mollova, Nevena</au><au>Leung, Kwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive and cost-effective LC–MS/MS method for quantitation of CVT-6883 in human urine using sodium dodecylbenzenesulfonate additive to eliminate adsorptive losses</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>877</volume><issue>10</issue><spage>943</spage><epage>947</epage><pages>943-947</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>CVT-6883, a novel selective A
2B adenosine receptor antagonist currently under clinical development, is highly lipophilic and exhibits high affinity for non-specific binding to container surfaces, resulting in very low recovery in urine assays. Our study showed the use of sodium dodecylbenzenesulfonate (SDBS), a low-cost additive, eliminated non-specific binding problems in the analysis of CVT-6883 in human urine without compromising sensitivity. A new sensitive and selective LC–MS/MS method for quantitation of CVT-6883 in the range of 0.200–80.0
ng/mL using SDBS additive was therefore developed and validated for the analysis of human urine samples. The recoveries during sample collection, handling and extraction for the analyte and internal standard (
d
5-CVT-6883) were higher than 87%. CVT-6883 was found stable under the following conditions: in extract – at ambient temperature for 3 days, under refrigeration (5
°C) for 6 days; in human urine (containing 4
mM SDBS) – after three freeze/thaw cycles, at ambient temperature for 26
h, under refrigeration (5
°C) for 94
h, and in a freezer set to −20
°C for at least 2 months. The results demonstrated that the validated method is sufficiently sensitive, specific, and cost-effective for the analysis of CVT-6883 in human urine and will provide a powerful tool to support the clinical programs for CVT-6883.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19282253</pmid><doi>10.1016/j.jchromb.2009.02.045</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2009-04, Vol.877 (10), p.943-947 |
| issn | 1570-0232 1873-376X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_903647808 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | A 2B adenosin receptor antagonist Adsorption Adsorptive losses Analysis Analytical, structural and metabolic biochemistry Benzenesulfonates - chemistry Biological and medical sciences Chromatography, High Pressure Liquid - economics Chromatography, High Pressure Liquid - methods CVT-6883 Fundamental and applied biological sciences. Psychology General pharmacology Human urine Humans LC–MS/MS Medical sciences Method validation Non-specific binding Pharmacology. Drug treatments Purines - urine Pyrazoles - urine Reference Standards SDBS Sodium dodecylbenzenesulfonate Tandem Mass Spectrometry - economics Tandem Mass Spectrometry - methods |
| title | Sensitive and cost-effective LC–MS/MS method for quantitation of CVT-6883 in human urine using sodium dodecylbenzenesulfonate additive to eliminate adsorptive losses |
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