Quantitative determination of the 1,3-butadiene urinary metabolite 1,2-dihydroxybutyl mercapturic acid by high-performance liquid chromatography/tandem mass spectrometry using polynomial calibration curves
1,3-Butadiene is used in the production of synthetic rubber and is also a widespread environmental pollutant, produced by car exhaust, heating and cigarette smoke. According to IARC it is probably carcinogenic to humans. A method was developed and validated for the quantification in human urine of 1...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-05, Vol.877 (13), p.1388-1393 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Carrieri, Mariella Bartolucci, Giovanni Battista Livieri, Monica Paci, Enrico Pigini, Daniela Sisto, Renata Corsetti, Federica Tranfo, Giovanna |
| description | 1,3-Butadiene is used in the production of synthetic rubber and is also a widespread environmental pollutant, produced by car exhaust, heating and cigarette smoke. According to IARC it is probably carcinogenic to humans. A method was developed and validated for the quantification in human urine of 1,2-dihydroxybutyl mercapturic acid, a butadiene metabolite for which the American Conference of Governmental Hygienists suggests a biological exposure index of 2500
μg/L. Solid phase extraction was used for analyte extraction and HPLC–MS/MS for detection. The calibration range from 20 to 2500
μg/L required the use of polynomial calibration curves, and the performance of the analytical method was tested according to an international validation guideline. Accuracy was never less than 85%, precision always higher than 15% and the LOD 3.6
μg/L. The method was applied to 33 non-smokers, non-occupationally exposed to butadiene, and gave urinary concentrations between 16 and 599
μg/L. |
| doi_str_mv | 10.1016/j.jchromb.2009.02.005 |
| format | Article |
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μg/L. Solid phase extraction was used for analyte extraction and HPLC–MS/MS for detection. The calibration range from 20 to 2500
μg/L required the use of polynomial calibration curves, and the performance of the analytical method was tested according to an international validation guideline. Accuracy was never less than 85%, precision always higher than 15% and the LOD 3.6
μg/L. The method was applied to 33 non-smokers, non-occupationally exposed to butadiene, and gave urinary concentrations between 16 and 599
μg/L.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2009.02.005</identifier><identifier>PMID: 19233748</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>1,3-Butadiene ; Acetylcysteine - analogs & derivatives ; Acetylcysteine - urine ; Biological monitoring ; Butadienes - urine ; Calibration ; Chromatography, High Pressure Liquid - methods ; DHBMA ; HPLC–MS/MS ; Humans ; Method validation ; Occupational exposure ; Polynomial regression ; Quality Control ; Reproducibility of Results ; Sensitivity and Specificity ; Tandem Mass Spectrometry - methods ; Uncertainty</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2009-05, Vol.877 (13), p.1388-1393</ispartof><rights>2009 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-90af8d1b6b33033f2f3ce09d31d21fa9040da1a261c11db954b3209b3c5393ef3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2009.02.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19233748$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carrieri, Mariella</creatorcontrib><creatorcontrib>Bartolucci, Giovanni Battista</creatorcontrib><creatorcontrib>Livieri, Monica</creatorcontrib><creatorcontrib>Paci, Enrico</creatorcontrib><creatorcontrib>Pigini, Daniela</creatorcontrib><creatorcontrib>Sisto, Renata</creatorcontrib><creatorcontrib>Corsetti, Federica</creatorcontrib><creatorcontrib>Tranfo, Giovanna</creatorcontrib><title>Quantitative determination of the 1,3-butadiene urinary metabolite 1,2-dihydroxybutyl mercapturic acid by high-performance liquid chromatography/tandem mass spectrometry using polynomial calibration curves</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>1,3-Butadiene is used in the production of synthetic rubber and is also a widespread environmental pollutant, produced by car exhaust, heating and cigarette smoke. According to IARC it is probably carcinogenic to humans. A method was developed and validated for the quantification in human urine of 1,2-dihydroxybutyl mercapturic acid, a butadiene metabolite for which the American Conference of Governmental Hygienists suggests a biological exposure index of 2500
μg/L. Solid phase extraction was used for analyte extraction and HPLC–MS/MS for detection. The calibration range from 20 to 2500
μg/L required the use of polynomial calibration curves, and the performance of the analytical method was tested according to an international validation guideline. Accuracy was never less than 85%, precision always higher than 15% and the LOD 3.6
μg/L. The method was applied to 33 non-smokers, non-occupationally exposed to butadiene, and gave urinary concentrations between 16 and 599
μg/L.</description><subject>1,3-Butadiene</subject><subject>Acetylcysteine - analogs & derivatives</subject><subject>Acetylcysteine - urine</subject><subject>Biological monitoring</subject><subject>Butadienes - urine</subject><subject>Calibration</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>DHBMA</subject><subject>HPLC–MS/MS</subject><subject>Humans</subject><subject>Method validation</subject><subject>Occupational exposure</subject><subject>Polynomial regression</subject><subject>Quality Control</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Uncertainty</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhQtRnIf-BCUr3Uz13CT16KxkGHzBgAgK7kIet7rSVFVqklRj_Uj_k2m7wZ2uknC-m5OcUxSvKGwo0OZ2v9mbPvhRbxiA2ADbANRPiku6bXnJ2-bH07yvWyiBcXZRXMW4B6AttPx5cUEF47yttpfFr6-LmpJLKrkDEosJw-imfPIT8R1JPRJ6w0u9JGUdTkiWkOWwkhGT0n5w6Qiw0rp-tcH_XDO5DlkNRs0pw4Yo4yzRK-ndri9nDJ0Po5oMksE9Lln68w2V_C6ouV9vk5osjmRUMZI4o0lZxZQdl-imHZn9sE5-dGogRg1Oh9NbzRIOGF8Uzzo1RHx5Xq-L7x_ef7v_VD58-fj5_u6hNFVdp1KA6raW6kZzDpx3rOMGQVhOLaOdElCBVVSxhhpKrRZ1pTkDobmpueDY8evi7eneOfjHBWOSo4sGh0FN6JcoBfCmAspZJt_8k-RVBYKKKoP1CTTBxxiwk3NwY05aUpDHxuVenhuXx8YlMJkbz3OvzwaLHtH-nTpXnIF3JwBzIAeHQUaTmzRoXcjpSuvdfyx-A4urxj4</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Carrieri, Mariella</creator><creator>Bartolucci, Giovanni Battista</creator><creator>Livieri, Monica</creator><creator>Paci, Enrico</creator><creator>Pigini, Daniela</creator><creator>Sisto, Renata</creator><creator>Corsetti, Federica</creator><creator>Tranfo, Giovanna</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope></search><sort><creationdate>20090501</creationdate><title>Quantitative determination of the 1,3-butadiene urinary metabolite 1,2-dihydroxybutyl mercapturic acid by high-performance liquid chromatography/tandem mass spectrometry using polynomial calibration curves</title><author>Carrieri, Mariella ; Bartolucci, Giovanni Battista ; Livieri, Monica ; Paci, Enrico ; Pigini, Daniela ; Sisto, Renata ; Corsetti, Federica ; Tranfo, Giovanna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-90af8d1b6b33033f2f3ce09d31d21fa9040da1a261c11db954b3209b3c5393ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>1,3-Butadiene</topic><topic>Acetylcysteine - analogs & derivatives</topic><topic>Acetylcysteine - urine</topic><topic>Biological monitoring</topic><topic>Butadienes - urine</topic><topic>Calibration</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>DHBMA</topic><topic>HPLC–MS/MS</topic><topic>Humans</topic><topic>Method validation</topic><topic>Occupational exposure</topic><topic>Polynomial regression</topic><topic>Quality Control</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Uncertainty</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carrieri, Mariella</creatorcontrib><creatorcontrib>Bartolucci, Giovanni Battista</creatorcontrib><creatorcontrib>Livieri, Monica</creatorcontrib><creatorcontrib>Paci, Enrico</creatorcontrib><creatorcontrib>Pigini, Daniela</creatorcontrib><creatorcontrib>Sisto, Renata</creatorcontrib><creatorcontrib>Corsetti, Federica</creatorcontrib><creatorcontrib>Tranfo, Giovanna</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><jtitle>Journal of chromatography. 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μg/L. Solid phase extraction was used for analyte extraction and HPLC–MS/MS for detection. The calibration range from 20 to 2500
μg/L required the use of polynomial calibration curves, and the performance of the analytical method was tested according to an international validation guideline. Accuracy was never less than 85%, precision always higher than 15% and the LOD 3.6
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| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2009-05, Vol.877 (13), p.1388-1393 |
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| subjects | 1,3-Butadiene Acetylcysteine - analogs & derivatives Acetylcysteine - urine Biological monitoring Butadienes - urine Calibration Chromatography, High Pressure Liquid - methods DHBMA HPLC–MS/MS Humans Method validation Occupational exposure Polynomial regression Quality Control Reproducibility of Results Sensitivity and Specificity Tandem Mass Spectrometry - methods Uncertainty |
| title | Quantitative determination of the 1,3-butadiene urinary metabolite 1,2-dihydroxybutyl mercapturic acid by high-performance liquid chromatography/tandem mass spectrometry using polynomial calibration curves |
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