Non-enzymatic aqueous peroxyoxalate chemiluminescence immune detection using a CCD camera and a CMOS device
A new method for non‐enzymatic aqueous peroxyoxalate chemiluminescence (POCL) biomolecular detection using imaging chip‐based devices has been developed. A water‐soluble amide of oxalic acid was synthesized and used in the investigation and characterization of POCL immunodetection in an aqueous envi...
Gespeichert in:
Veröffentlicht in: | Luminescence 2008-10, Vol.23 (5), p.296-302 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 302 |
---|---|
container_issue | 5 |
container_start_page | 296 |
container_title | Luminescence |
container_volume | 23 |
creator | Filanoski, Brian Rastogi, Shiva K. Cameron, Adam Cameron, Eric Mishra, Nirankar N. Maki, Wusi Maki, Gary |
description | A new method for non‐enzymatic aqueous peroxyoxalate chemiluminescence (POCL) biomolecular detection using imaging chip‐based devices has been developed. A water‐soluble amide of oxalic acid was synthesized and used in the investigation and characterization of POCL immunodetection in an aqueous environment. Six fluorescent dyes commonly used in biological detection were tested, and the intensity of light generated from the aqueous POCL reactions was characterized in the liquid phase. Direct detection sensitivity comparisons between a standard fluorescent method and this POCL method were performed in both liquid and solid phases. Results showed that detection sensitivity using the POCL method is comparable to that of the fluorescent method. POCL biomolecular detection on a nitrocellulose membrane was also investigated using a charge‐coupled device (CCD) camera. Again, POCL detection sensitivity proved to be comparable to that using the fluorescent detection method. In an application of aqueous POCL biomolecular detection, Staphylococcus aureus enterotoxin B (SEB) and its antibody were used to demonstrate immuno‐ and affinity detection. For further applications, such as DNA and protein arrays, simultaneous detection of biomolecules labelled with different fluorescent labels was investigated, using a complementary metal oxide semiconductor (CMOS) colour imaging chip. Copyright © 2008 John Wiley & Sons, Ltd. |
doi_str_mv | 10.1002/bio.1033 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_903635441</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20746569</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4823-662d1ceb72cd69071ae2605546d97489b27deb5da324690864646b6b0d80767c3</originalsourceid><addsrcrecordid>eNqN0ctu1DAUBuAIUdFSkHgC5BWwCfXd8ZIZoFRqZ5C4LS3HPgXTOJnGCczw9DiaqF3RYi98ZH_65aNTFM8Ifk0wpid16HLB2IPiiAhKS0U5e3hTM3FYPE7pJ8ZYSqkfFYek4oISjo-Kq1XXltD-2UU7BIfs9QjdmNAG-m6767a2sQMg9wNiaMYYWkgOWgcoxDi2gDwM4IbQtWhMof2OLFou3yJnI_QW2dZPFxfrT9n9Cg6eFAeXtknwdD6Piy_v331efijP16dnyzfnpeMVZaWU1BMHtaLOS40VsUAlFoJLrxWvdE2Vh1p4yyjP75Xkedeyxr7CSirHjouX-9xN3-V-0mBiyP9uGttOzRmNmWSCc5Llizul1EJrosS9kHFdEcGq_4DTovheSLHiUkid4as9dH2XUg-XZtOHaPudIdhM0zd5-maafqbP58yxjuBv4TzuDMo9-B0a2P0zyCzO1nPg7EMaYHvjbX9lpGJKmG-rU3OhFgv2cfXVSPYXrw7FzQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20746569</pqid></control><display><type>article</type><title>Non-enzymatic aqueous peroxyoxalate chemiluminescence immune detection using a CCD camera and a CMOS device</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Filanoski, Brian ; Rastogi, Shiva K. ; Cameron, Adam ; Cameron, Eric ; Mishra, Nirankar N. ; Maki, Wusi ; Maki, Gary</creator><creatorcontrib>Filanoski, Brian ; Rastogi, Shiva K. ; Cameron, Adam ; Cameron, Eric ; Mishra, Nirankar N. ; Maki, Wusi ; Maki, Gary</creatorcontrib><description>A new method for non‐enzymatic aqueous peroxyoxalate chemiluminescence (POCL) biomolecular detection using imaging chip‐based devices has been developed. A water‐soluble amide of oxalic acid was synthesized and used in the investigation and characterization of POCL immunodetection in an aqueous environment. Six fluorescent dyes commonly used in biological detection were tested, and the intensity of light generated from the aqueous POCL reactions was characterized in the liquid phase. Direct detection sensitivity comparisons between a standard fluorescent method and this POCL method were performed in both liquid and solid phases. Results showed that detection sensitivity using the POCL method is comparable to that of the fluorescent method. POCL biomolecular detection on a nitrocellulose membrane was also investigated using a charge‐coupled device (CCD) camera. Again, POCL detection sensitivity proved to be comparable to that using the fluorescent detection method. In an application of aqueous POCL biomolecular detection, Staphylococcus aureus enterotoxin B (SEB) and its antibody were used to demonstrate immuno‐ and affinity detection. For further applications, such as DNA and protein arrays, simultaneous detection of biomolecules labelled with different fluorescent labels was investigated, using a complementary metal oxide semiconductor (CMOS) colour imaging chip. Copyright © 2008 John Wiley & Sons, Ltd.</description><identifier>ISSN: 1522-7235</identifier><identifier>EISSN: 1522-7243</identifier><identifier>EISSN: 1099-1271</identifier><identifier>DOI: 10.1002/bio.1033</identifier><identifier>PMID: 18452140</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>aqueous ; CMOS device ; Equipment Design ; Fluorescent Dyes ; immunoassay ; Immunoassay - methods ; Immunoassay - standards ; Luminescence ; Luminescent Measurements - instrumentation ; Luminescent Measurements - methods ; Oxalates - chemistry ; peroxyoxalate chemiluminescence ; Staphylococcus aureus ; Staphylococcus aureus enterotoxin B</subject><ispartof>Luminescence, 2008-10, Vol.23 (5), p.296-302</ispartof><rights>Copyright © 2008 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4823-662d1ceb72cd69071ae2605546d97489b27deb5da324690864646b6b0d80767c3</citedby><cites>FETCH-LOGICAL-c4823-662d1ceb72cd69071ae2605546d97489b27deb5da324690864646b6b0d80767c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbio.1033$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbio.1033$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18452140$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Filanoski, Brian</creatorcontrib><creatorcontrib>Rastogi, Shiva K.</creatorcontrib><creatorcontrib>Cameron, Adam</creatorcontrib><creatorcontrib>Cameron, Eric</creatorcontrib><creatorcontrib>Mishra, Nirankar N.</creatorcontrib><creatorcontrib>Maki, Wusi</creatorcontrib><creatorcontrib>Maki, Gary</creatorcontrib><title>Non-enzymatic aqueous peroxyoxalate chemiluminescence immune detection using a CCD camera and a CMOS device</title><title>Luminescence</title><addtitle>Luminescence</addtitle><description>A new method for non‐enzymatic aqueous peroxyoxalate chemiluminescence (POCL) biomolecular detection using imaging chip‐based devices has been developed. A water‐soluble amide of oxalic acid was synthesized and used in the investigation and characterization of POCL immunodetection in an aqueous environment. Six fluorescent dyes commonly used in biological detection were tested, and the intensity of light generated from the aqueous POCL reactions was characterized in the liquid phase. Direct detection sensitivity comparisons between a standard fluorescent method and this POCL method were performed in both liquid and solid phases. Results showed that detection sensitivity using the POCL method is comparable to that of the fluorescent method. POCL biomolecular detection on a nitrocellulose membrane was also investigated using a charge‐coupled device (CCD) camera. Again, POCL detection sensitivity proved to be comparable to that using the fluorescent detection method. In an application of aqueous POCL biomolecular detection, Staphylococcus aureus enterotoxin B (SEB) and its antibody were used to demonstrate immuno‐ and affinity detection. For further applications, such as DNA and protein arrays, simultaneous detection of biomolecules labelled with different fluorescent labels was investigated, using a complementary metal oxide semiconductor (CMOS) colour imaging chip. Copyright © 2008 John Wiley & Sons, Ltd.</description><subject>aqueous</subject><subject>CMOS device</subject><subject>Equipment Design</subject><subject>Fluorescent Dyes</subject><subject>immunoassay</subject><subject>Immunoassay - methods</subject><subject>Immunoassay - standards</subject><subject>Luminescence</subject><subject>Luminescent Measurements - instrumentation</subject><subject>Luminescent Measurements - methods</subject><subject>Oxalates - chemistry</subject><subject>peroxyoxalate chemiluminescence</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus enterotoxin B</subject><issn>1522-7235</issn><issn>1522-7243</issn><issn>1099-1271</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0ctu1DAUBuAIUdFSkHgC5BWwCfXd8ZIZoFRqZ5C4LS3HPgXTOJnGCczw9DiaqF3RYi98ZH_65aNTFM8Ifk0wpid16HLB2IPiiAhKS0U5e3hTM3FYPE7pJ8ZYSqkfFYek4oISjo-Kq1XXltD-2UU7BIfs9QjdmNAG-m6767a2sQMg9wNiaMYYWkgOWgcoxDi2gDwM4IbQtWhMof2OLFou3yJnI_QW2dZPFxfrT9n9Cg6eFAeXtknwdD6Piy_v331efijP16dnyzfnpeMVZaWU1BMHtaLOS40VsUAlFoJLrxWvdE2Vh1p4yyjP75Xkedeyxr7CSirHjouX-9xN3-V-0mBiyP9uGttOzRmNmWSCc5Llizul1EJrosS9kHFdEcGq_4DTovheSLHiUkid4as9dH2XUg-XZtOHaPudIdhM0zd5-maafqbP58yxjuBv4TzuDMo9-B0a2P0zyCzO1nPg7EMaYHvjbX9lpGJKmG-rU3OhFgv2cfXVSPYXrw7FzQ</recordid><startdate>200810</startdate><enddate>200810</enddate><creator>Filanoski, Brian</creator><creator>Rastogi, Shiva K.</creator><creator>Cameron, Adam</creator><creator>Cameron, Eric</creator><creator>Mishra, Nirankar N.</creator><creator>Maki, Wusi</creator><creator>Maki, Gary</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>200810</creationdate><title>Non-enzymatic aqueous peroxyoxalate chemiluminescence immune detection using a CCD camera and a CMOS device</title><author>Filanoski, Brian ; Rastogi, Shiva K. ; Cameron, Adam ; Cameron, Eric ; Mishra, Nirankar N. ; Maki, Wusi ; Maki, Gary</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4823-662d1ceb72cd69071ae2605546d97489b27deb5da324690864646b6b0d80767c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>aqueous</topic><topic>CMOS device</topic><topic>Equipment Design</topic><topic>Fluorescent Dyes</topic><topic>immunoassay</topic><topic>Immunoassay - methods</topic><topic>Immunoassay - standards</topic><topic>Luminescence</topic><topic>Luminescent Measurements - instrumentation</topic><topic>Luminescent Measurements - methods</topic><topic>Oxalates - chemistry</topic><topic>peroxyoxalate chemiluminescence</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus enterotoxin B</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Filanoski, Brian</creatorcontrib><creatorcontrib>Rastogi, Shiva K.</creatorcontrib><creatorcontrib>Cameron, Adam</creatorcontrib><creatorcontrib>Cameron, Eric</creatorcontrib><creatorcontrib>Mishra, Nirankar N.</creatorcontrib><creatorcontrib>Maki, Wusi</creatorcontrib><creatorcontrib>Maki, Gary</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Luminescence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Filanoski, Brian</au><au>Rastogi, Shiva K.</au><au>Cameron, Adam</au><au>Cameron, Eric</au><au>Mishra, Nirankar N.</au><au>Maki, Wusi</au><au>Maki, Gary</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Non-enzymatic aqueous peroxyoxalate chemiluminescence immune detection using a CCD camera and a CMOS device</atitle><jtitle>Luminescence</jtitle><addtitle>Luminescence</addtitle><date>2008-10</date><risdate>2008</risdate><volume>23</volume><issue>5</issue><spage>296</spage><epage>302</epage><pages>296-302</pages><issn>1522-7235</issn><eissn>1522-7243</eissn><eissn>1099-1271</eissn><abstract>A new method for non‐enzymatic aqueous peroxyoxalate chemiluminescence (POCL) biomolecular detection using imaging chip‐based devices has been developed. A water‐soluble amide of oxalic acid was synthesized and used in the investigation and characterization of POCL immunodetection in an aqueous environment. Six fluorescent dyes commonly used in biological detection were tested, and the intensity of light generated from the aqueous POCL reactions was characterized in the liquid phase. Direct detection sensitivity comparisons between a standard fluorescent method and this POCL method were performed in both liquid and solid phases. Results showed that detection sensitivity using the POCL method is comparable to that of the fluorescent method. POCL biomolecular detection on a nitrocellulose membrane was also investigated using a charge‐coupled device (CCD) camera. Again, POCL detection sensitivity proved to be comparable to that using the fluorescent detection method. In an application of aqueous POCL biomolecular detection, Staphylococcus aureus enterotoxin B (SEB) and its antibody were used to demonstrate immuno‐ and affinity detection. For further applications, such as DNA and protein arrays, simultaneous detection of biomolecules labelled with different fluorescent labels was investigated, using a complementary metal oxide semiconductor (CMOS) colour imaging chip. Copyright © 2008 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>18452140</pmid><doi>10.1002/bio.1033</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1522-7235 |
ispartof | Luminescence, 2008-10, Vol.23 (5), p.296-302 |
issn | 1522-7235 1522-7243 1099-1271 |
language | eng |
recordid | cdi_proquest_miscellaneous_903635441 |
source | MEDLINE; Wiley Online Library All Journals |
subjects | aqueous CMOS device Equipment Design Fluorescent Dyes immunoassay Immunoassay - methods Immunoassay - standards Luminescence Luminescent Measurements - instrumentation Luminescent Measurements - methods Oxalates - chemistry peroxyoxalate chemiluminescence Staphylococcus aureus Staphylococcus aureus enterotoxin B |
title | Non-enzymatic aqueous peroxyoxalate chemiluminescence immune detection using a CCD camera and a CMOS device |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T16%3A37%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Non-enzymatic%20aqueous%20peroxyoxalate%20chemiluminescence%20immune%20detection%20using%20a%20CCD%20camera%20and%20a%20CMOS%20device&rft.jtitle=Luminescence&rft.au=Filanoski,%20Brian&rft.date=2008-10&rft.volume=23&rft.issue=5&rft.spage=296&rft.epage=302&rft.pages=296-302&rft.issn=1522-7235&rft.eissn=1522-7243&rft_id=info:doi/10.1002/bio.1033&rft_dat=%3Cproquest_cross%3E20746569%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20746569&rft_id=info:pmid/18452140&rfr_iscdi=true |