Development of a Disposable All-Solid-State Ascorbic Acid Biosensor and Miniaturized Reference Electrode Fabricated on Single Substrate
A low-cost, small-size, and all-solid-state biosensor for the specific determination of L-ascorbic acid was developed. The sensor array was fabricated by screen printing, and the ruthenium oxide (RUO 2 ) thin film was deposited on the working area of each sensor by an RF sputtering method for surfac...
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| Veröffentlicht in: | IEEE sensors journal 2008-09, Vol.8 (9), p.1571-1577 |
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| creator | Jung Chuan Chou Tsai, Y.H. Chien Cheng Chen |
| description | A low-cost, small-size, and all-solid-state biosensor for the specific determination of L-ascorbic acid was developed. The sensor array was fabricated by screen printing, and the ruthenium oxide (RUO 2 ) thin film was deposited on the working area of each sensor by an RF sputtering method for surface modification. A conductive polymer (polypyrrole) miniaturized reference electrode was used in combination with the biosensor. The ascorbate oxidase was immobilized with 3-glycidoxypropyltrimethoxysilane (GPTS) methods in the bioactive membrane layer. In accordance with experiment results, the sensor has the better average sensitivity of 51.85 mV/pH between pH 1 and pH 13. The investigated characteristics in the polypyrrole film modified reference electrode show a very small drift voltage (5 mV/h) and the standard deviation of the reference electrode is 1.093 mV. According to the experiment results, the potential response of the enzyme-based biosensor depends linearly on L-ascorbic acid concentration between 0.02 mM and 1 mM with a linear correlation coefficient of 0.86 and the sensitivity is 13.85 mV/mM. |
| doi_str_mv | 10.1109/JSEN.2008.928923 |
| format | Article |
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The sensor array was fabricated by screen printing, and the ruthenium oxide (RUO 2 ) thin film was deposited on the working area of each sensor by an RF sputtering method for surface modification. A conductive polymer (polypyrrole) miniaturized reference electrode was used in combination with the biosensor. The ascorbate oxidase was immobilized with 3-glycidoxypropyltrimethoxysilane (GPTS) methods in the bioactive membrane layer. In accordance with experiment results, the sensor has the better average sensitivity of 51.85 mV/pH between pH 1 and pH 13. The investigated characteristics in the polypyrrole film modified reference electrode show a very small drift voltage (5 mV/h) and the standard deviation of the reference electrode is 1.093 mV. 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(IEEE) 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-29ce3f84382c3d57b091044698ccea3ac69e0c9186a8b7be4ea12c6eddd8dc623</citedby><cites>FETCH-LOGICAL-c482t-29ce3f84382c3d57b091044698ccea3ac69e0c9186a8b7be4ea12c6eddd8dc623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/4609972$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>314,780,784,796,27924,27925,54758</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/4609972$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc></links><search><creatorcontrib>Jung Chuan Chou</creatorcontrib><creatorcontrib>Tsai, Y.H.</creatorcontrib><creatorcontrib>Chien Cheng Chen</creatorcontrib><title>Development of a Disposable All-Solid-State Ascorbic Acid Biosensor and Miniaturized Reference Electrode Fabricated on Single Substrate</title><title>IEEE sensors journal</title><addtitle>JSEN</addtitle><description>A low-cost, small-size, and all-solid-state biosensor for the specific determination of L-ascorbic acid was developed. The sensor array was fabricated by screen printing, and the ruthenium oxide (RUO 2 ) thin film was deposited on the working area of each sensor by an RF sputtering method for surface modification. A conductive polymer (polypyrrole) miniaturized reference electrode was used in combination with the biosensor. The ascorbate oxidase was immobilized with 3-glycidoxypropyltrimethoxysilane (GPTS) methods in the bioactive membrane layer. In accordance with experiment results, the sensor has the better average sensitivity of 51.85 mV/pH between pH 1 and pH 13. The investigated characteristics in the polypyrrole film modified reference electrode show a very small drift voltage (5 mV/h) and the standard deviation of the reference electrode is 1.093 mV. According to the experiment results, the potential response of the enzyme-based biosensor depends linearly on L-ascorbic acid concentration between 0.02 mM and 1 mM with a linear correlation coefficient of 0.86 and the sensitivity is 13.85 mV/mM.</description><subject>Ascorbic acid</subject><subject>biosensor</subject><subject>Biosensors</subject><subject>Conductive films</subject><subject>Electrodes</subject><subject>Polymer films</subject><subject>polypyrrole</subject><subject>Printing</subject><subject>Radio frequency</subject><subject>ruthenium oxide</subject><subject>screen printed</subject><subject>Screen printing</subject><subject>Sensor arrays</subject><subject>Sputtering</subject><subject>Standard deviation</subject><subject>Studies</subject><subject>Substrates</subject><subject>Thin film sensors</subject><issn>1530-437X</issn><issn>1558-1748</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>RIE</sourceid><recordid>eNqF0kuLFDEQB_BGFFxX74KX4EFPPebVeRzH3VkfrAq2greQTqolSyYZk25Bv4Bf2wwjHjy4p1SKX1Ug_LvuMcEbQrB-8Xbcvd9QjNVGU6Upu9OdkWFQPZFc3T3WDPecyS_3uwe13mBMtBzkWffrEr5DzIc9pAXlGVl0GeohVztFQNsY-zHH4PtxsUu7V5fLFBzauuDRy5ArpJoLssmjdyEFu6wl_ASPPsIMBZIDtIvglpI9oCs7leDaGo9yQmNIX9sL4zrVpbTmw-7ebGOFR3_O8-7z1e7Txev--sOrNxfb695xRZeeagdsVpwp6pgf5IQ1wZwLrZwDy6wTGrDTRAmrJjkBB0uoE-C9V94Jys6756e9h5K_rVAXsw_VQYw2QV6r0ZgJSggXt0olByyJ4KzJZ_-VjHOsNJe3QooFoZyQBp_-A2_yWlL7GKME44oJqRvCJ-RKrrXAbA4l7G35YQg2x0yYYybMMRPmlIk28uQ0EgDgL-cCay0p-w2yFrND</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Jung Chuan Chou</creator><creator>Tsai, Y.H.</creator><creator>Chien Cheng Chen</creator><general>IEEE</general><general>The Institute of Electrical and Electronics Engineers, Inc. (IEEE)</general><scope>97E</scope><scope>RIA</scope><scope>RIE</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7QO</scope><scope>FR3</scope><scope>P64</scope><scope>F28</scope></search><sort><creationdate>20080901</creationdate><title>Development of a Disposable All-Solid-State Ascorbic Acid Biosensor and Miniaturized Reference Electrode Fabricated on Single Substrate</title><author>Jung Chuan Chou ; Tsai, Y.H. ; Chien Cheng Chen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-29ce3f84382c3d57b091044698ccea3ac69e0c9186a8b7be4ea12c6eddd8dc623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Ascorbic acid</topic><topic>biosensor</topic><topic>Biosensors</topic><topic>Conductive films</topic><topic>Electrodes</topic><topic>Polymer films</topic><topic>polypyrrole</topic><topic>Printing</topic><topic>Radio frequency</topic><topic>ruthenium oxide</topic><topic>screen printed</topic><topic>Screen printing</topic><topic>Sensor arrays</topic><topic>Sputtering</topic><topic>Standard deviation</topic><topic>Studies</topic><topic>Substrates</topic><topic>Thin film sensors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jung Chuan Chou</creatorcontrib><creatorcontrib>Tsai, Y.H.</creatorcontrib><creatorcontrib>Chien Cheng Chen</creatorcontrib><collection>IEEE All-Society Periodicals Package (ASPP) 2005-present</collection><collection>IEEE All-Society Periodicals Package (ASPP) 1998-Present</collection><collection>IEEE Electronic Library (IEL)</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology Research Abstracts</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><jtitle>IEEE sensors journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Jung Chuan Chou</au><au>Tsai, Y.H.</au><au>Chien Cheng Chen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a Disposable All-Solid-State Ascorbic Acid Biosensor and Miniaturized Reference Electrode Fabricated on Single Substrate</atitle><jtitle>IEEE sensors journal</jtitle><stitle>JSEN</stitle><date>2008-09-01</date><risdate>2008</risdate><volume>8</volume><issue>9</issue><spage>1571</spage><epage>1577</epage><pages>1571-1577</pages><issn>1530-437X</issn><eissn>1558-1748</eissn><coden>ISJEAZ</coden><abstract>A low-cost, small-size, and all-solid-state biosensor for the specific determination of L-ascorbic acid was developed. The sensor array was fabricated by screen printing, and the ruthenium oxide (RUO 2 ) thin film was deposited on the working area of each sensor by an RF sputtering method for surface modification. A conductive polymer (polypyrrole) miniaturized reference electrode was used in combination with the biosensor. The ascorbate oxidase was immobilized with 3-glycidoxypropyltrimethoxysilane (GPTS) methods in the bioactive membrane layer. In accordance with experiment results, the sensor has the better average sensitivity of 51.85 mV/pH between pH 1 and pH 13. The investigated characteristics in the polypyrrole film modified reference electrode show a very small drift voltage (5 mV/h) and the standard deviation of the reference electrode is 1.093 mV. According to the experiment results, the potential response of the enzyme-based biosensor depends linearly on L-ascorbic acid concentration between 0.02 mM and 1 mM with a linear correlation coefficient of 0.86 and the sensitivity is 13.85 mV/mM.</abstract><cop>New York</cop><pub>IEEE</pub><doi>10.1109/JSEN.2008.928923</doi><tpages>7</tpages></addata></record> |
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| subjects | Ascorbic acid biosensor Biosensors Conductive films Electrodes Polymer films polypyrrole Printing Radio frequency ruthenium oxide screen printed Screen printing Sensor arrays Sputtering Standard deviation Studies Substrates Thin film sensors |
| title | Development of a Disposable All-Solid-State Ascorbic Acid Biosensor and Miniaturized Reference Electrode Fabricated on Single Substrate |
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