Bioanalysis of recombinant proteins and antibodies by mass spectrometry
In recent years, biotechnologically-derived drugs have been a major focus of research and development in the pharmaceutical industry. Their pharmacokinetics and pharmacokinetic/pharmacodynamic relationships impact every stage of the development process and require their assessment in the circulation...
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| Veröffentlicht in: | Analyst (London) 2009-05, Vol.134 (5), p.825-834 |
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| container_title | Analyst (London) |
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| creator | EZAN, Eric DUBOIS, Mathieu BECHER, François |
| description | In recent years, biotechnologically-derived drugs have been a major focus of research and development in the pharmaceutical industry. Their pharmacokinetics and pharmacokinetic/pharmacodynamic relationships impact every stage of the development process and require their assessment in the circulation in preclinical species and in humans. To this end, immunoassays are a reference, but standardisation remains an issue owing to the restricted pattern of antibody specificity and interference with endogenous components. As an alternative, we report here analytical strategies involving liquid chromatography coupled to mass spectrometry (LC-MS) for the accurate quantification of therapeutic proteins and antibodies in biological fluids. |
| doi_str_mv | 10.1039/b819706g |
| format | Article |
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| ispartof | Analyst (London), 2009-05, Vol.134 (5), p.825-834 |
| issn | 0003-2654 1364-5528 |
| language | eng |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Analytical chemistry Antibodies - analysis Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Liquid - methods Exact sciences and technology Immunoassay Mass Spectrometry - methods Miscellaneous Other chromatographic methods Recombinant Proteins - analysis Sensitivity and Specificity Spectrometric and optical methods |
| title | Bioanalysis of recombinant proteins and antibodies by mass spectrometry |
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