Natural antioxidants and vascular calcification: a possible benefit
Several studies have demonstrated the impact of vascular calcification on morbidity and mortality both in the general and chronic kidney disease populations. The process of vascular calcification involves complex mechanisms including the overexpression of genes and proteins associated with mineraliz...
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| Veröffentlicht in: | Journal of nephrology 2011-11, Vol.24 (6), p.669-672 |
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| container_title | Journal of nephrology |
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| creator | Roman-Garcia, Pablo Barrio-Vazquez, Sara Fernandez-Martin, Jose Luis Ruiz-Torres, Maria Piedad Cannata-Andia, Jorge B |
| description | Several studies have demonstrated the impact of vascular calcification on morbidity and mortality both in the general and chronic kidney disease populations. The process of vascular calcification involves complex mechanisms including the overexpression of genes and proteins associated with mineralization and increments of reactive oxygen species (ROS). Taking into account previous findings, we decided to analyze in vitro the likely inhibitory effect of natural antioxidants in the process of vascular calcification.
Primary vascular smooth muscle cells (VSMCs) were cultured with either normal medium or normal medium supplemented with calcium and phosphorus (P + Ca) in combination with several antioxidants. Mineralization, intracellular reactive oxygen species levels and the protein expression of Cbfa1/RUNX2 and Mn-superoxide dismutase-2 (SOD-2) were investigated.
Curcumin and silybin were the more effective, inhibiting both ROS increase and VSMC mineralization. Curcumin was able to prevent the increase in Cbfa1/RUNX2 expression, but did not modify SOD-2 expression in the VSMCs cultured with the P + Ca medium.
These findings support the importance of performing further studies in this field, as some antioxidants might have potential benefits in the management of vascular calcification. |
| doi_str_mv | 10.5301/jn.5000029 |
| format | Article |
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Primary vascular smooth muscle cells (VSMCs) were cultured with either normal medium or normal medium supplemented with calcium and phosphorus (P + Ca) in combination with several antioxidants. Mineralization, intracellular reactive oxygen species levels and the protein expression of Cbfa1/RUNX2 and Mn-superoxide dismutase-2 (SOD-2) were investigated.
Curcumin and silybin were the more effective, inhibiting both ROS increase and VSMC mineralization. Curcumin was able to prevent the increase in Cbfa1/RUNX2 expression, but did not modify SOD-2 expression in the VSMCs cultured with the P + Ca medium.
These findings support the importance of performing further studies in this field, as some antioxidants might have potential benefits in the management of vascular calcification.</description><identifier>ISSN: 1121-8428</identifier><identifier>EISSN: 1724-6059</identifier><identifier>DOI: 10.5301/jn.5000029</identifier><identifier>PMID: 21928237</identifier><language>eng</language><publisher>Italy</publisher><subject>Animals ; Antioxidants - pharmacology ; Antioxidants - therapeutic use ; Calcium - pharmacology ; Cells, Cultured ; Core Binding Factor Alpha 1 Subunit - metabolism ; Curcumin - pharmacology ; Curcumin - therapeutic use ; In Vitro Techniques ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - metabolism ; Phosphorus - pharmacology ; Rats ; Rats, Wistar ; Reactive Oxygen Species - metabolism ; Silymarin - pharmacology ; Silymarin - therapeutic use ; Superoxide Dismutase - metabolism ; Vascular Calcification - metabolism</subject><ispartof>Journal of nephrology, 2011-11, Vol.24 (6), p.669-672</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c286t-22eb71cb916738edf7da09dc21018a45c44ab7462d0ed88f5571ce0e2849a1ea3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21928237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roman-Garcia, Pablo</creatorcontrib><creatorcontrib>Barrio-Vazquez, Sara</creatorcontrib><creatorcontrib>Fernandez-Martin, Jose Luis</creatorcontrib><creatorcontrib>Ruiz-Torres, Maria Piedad</creatorcontrib><creatorcontrib>Cannata-Andia, Jorge B</creatorcontrib><title>Natural antioxidants and vascular calcification: a possible benefit</title><title>Journal of nephrology</title><addtitle>J Nephrol</addtitle><description>Several studies have demonstrated the impact of vascular calcification on morbidity and mortality both in the general and chronic kidney disease populations. The process of vascular calcification involves complex mechanisms including the overexpression of genes and proteins associated with mineralization and increments of reactive oxygen species (ROS). Taking into account previous findings, we decided to analyze in vitro the likely inhibitory effect of natural antioxidants in the process of vascular calcification.
Primary vascular smooth muscle cells (VSMCs) were cultured with either normal medium or normal medium supplemented with calcium and phosphorus (P + Ca) in combination with several antioxidants. Mineralization, intracellular reactive oxygen species levels and the protein expression of Cbfa1/RUNX2 and Mn-superoxide dismutase-2 (SOD-2) were investigated.
Curcumin and silybin were the more effective, inhibiting both ROS increase and VSMC mineralization. Curcumin was able to prevent the increase in Cbfa1/RUNX2 expression, but did not modify SOD-2 expression in the VSMCs cultured with the P + Ca medium.
These findings support the importance of performing further studies in this field, as some antioxidants might have potential benefits in the management of vascular calcification.</description><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Antioxidants - therapeutic use</subject><subject>Calcium - pharmacology</subject><subject>Cells, Cultured</subject><subject>Core Binding Factor Alpha 1 Subunit - metabolism</subject><subject>Curcumin - pharmacology</subject><subject>Curcumin - therapeutic use</subject><subject>In Vitro Techniques</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Phosphorus - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Silymarin - pharmacology</subject><subject>Silymarin - therapeutic use</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Vascular Calcification - metabolism</subject><issn>1121-8428</issn><issn>1724-6059</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMorq5e_AHSmyB0TaZJm3qTxS9Y9KLnMk2mkCXbrkkr-u-N7Opc3hl4eBkexi4EX6iCi5t1v1A8DdQH7ERUIPOSq_ow7QJEriXoGTuNcZ0IpUAesxmIGjQU1QlbvuA4BfQZ9qMbvpxNGdNhs0-MZvIYMoPeuM4ZTEB_m2G2HWJ0raespZ46N56xow59pPN9ztn7w_3b8ilfvT4-L-9WuQFdjjkAtZUwbS3KqtBku8oir60BwYVGqYyU2FayBMvJat0plWjiBFrWKAiLObva9W7D8DFRHJuNi4a8x56GKTY1h9QMUibyekeakH4N1DXb4DYYvhvBm19nzbpv9s4SfLmvndoN2X_0T1LxAznRZtA</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Roman-Garcia, Pablo</creator><creator>Barrio-Vazquez, Sara</creator><creator>Fernandez-Martin, Jose Luis</creator><creator>Ruiz-Torres, Maria Piedad</creator><creator>Cannata-Andia, Jorge B</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111101</creationdate><title>Natural antioxidants and vascular calcification: a possible benefit</title><author>Roman-Garcia, Pablo ; Barrio-Vazquez, Sara ; Fernandez-Martin, Jose Luis ; Ruiz-Torres, Maria Piedad ; Cannata-Andia, Jorge B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c286t-22eb71cb916738edf7da09dc21018a45c44ab7462d0ed88f5571ce0e2849a1ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Antioxidants - therapeutic use</topic><topic>Calcium - pharmacology</topic><topic>Cells, Cultured</topic><topic>Core Binding Factor Alpha 1 Subunit - metabolism</topic><topic>Curcumin - pharmacology</topic><topic>Curcumin - therapeutic use</topic><topic>In Vitro Techniques</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Phosphorus - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Silymarin - pharmacology</topic><topic>Silymarin - therapeutic use</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Vascular Calcification - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roman-Garcia, Pablo</creatorcontrib><creatorcontrib>Barrio-Vazquez, Sara</creatorcontrib><creatorcontrib>Fernandez-Martin, Jose Luis</creatorcontrib><creatorcontrib>Ruiz-Torres, Maria Piedad</creatorcontrib><creatorcontrib>Cannata-Andia, Jorge B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of nephrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roman-Garcia, Pablo</au><au>Barrio-Vazquez, Sara</au><au>Fernandez-Martin, Jose Luis</au><au>Ruiz-Torres, Maria Piedad</au><au>Cannata-Andia, Jorge B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Natural antioxidants and vascular calcification: a possible benefit</atitle><jtitle>Journal of nephrology</jtitle><addtitle>J Nephrol</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>24</volume><issue>6</issue><spage>669</spage><epage>672</epage><pages>669-672</pages><issn>1121-8428</issn><eissn>1724-6059</eissn><abstract>Several studies have demonstrated the impact of vascular calcification on morbidity and mortality both in the general and chronic kidney disease populations. The process of vascular calcification involves complex mechanisms including the overexpression of genes and proteins associated with mineralization and increments of reactive oxygen species (ROS). Taking into account previous findings, we decided to analyze in vitro the likely inhibitory effect of natural antioxidants in the process of vascular calcification.
Primary vascular smooth muscle cells (VSMCs) were cultured with either normal medium or normal medium supplemented with calcium and phosphorus (P + Ca) in combination with several antioxidants. Mineralization, intracellular reactive oxygen species levels and the protein expression of Cbfa1/RUNX2 and Mn-superoxide dismutase-2 (SOD-2) were investigated.
Curcumin and silybin were the more effective, inhibiting both ROS increase and VSMC mineralization. Curcumin was able to prevent the increase in Cbfa1/RUNX2 expression, but did not modify SOD-2 expression in the VSMCs cultured with the P + Ca medium.
These findings support the importance of performing further studies in this field, as some antioxidants might have potential benefits in the management of vascular calcification.</abstract><cop>Italy</cop><pmid>21928237</pmid><doi>10.5301/jn.5000029</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antioxidants - pharmacology Antioxidants - therapeutic use Calcium - pharmacology Cells, Cultured Core Binding Factor Alpha 1 Subunit - metabolism Curcumin - pharmacology Curcumin - therapeutic use In Vitro Techniques Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Phosphorus - pharmacology Rats Rats, Wistar Reactive Oxygen Species - metabolism Silymarin - pharmacology Silymarin - therapeutic use Superoxide Dismutase - metabolism Vascular Calcification - metabolism |
| title | Natural antioxidants and vascular calcification: a possible benefit |
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